Surface-enhanced Raman Scattering Sensor Research Articles

Gold Nanopolyhedron-Based Superlattice Sheets as Flexible Surface-Enhanced Raman Scattering Sensors for Detection of 4-Aminothiophenol

Gold Nanopolyhedron-Based Superlattice Sheets as Flexible Surface-Enhanced Raman Scattering Sensors for Detection of 4-Aminothiophenol

Sub-Part-Per-Billion Level Sensing of Fentanyl Residues from Wastewater Using Portable Surface-Enhanced Raman Scattering Sensing.

Detection of illicit drug residues from wastewater provides a new route toward community-level assessment of drug abuse that is critical to public health. However, traditional chemistry analytical tools such as high-performance liquid chromatography in tandem with mass spectrometry (HPLC-MS) cannot meet the large-scale testing requirement in terms of cost, promptness, and convenience of use. In this article, we demonstrated ultra-sensitive and portable surface-enhanced Raman scattering sensing (SERS) of fentanyl, a synthetic opioid, from sewage water and achieved quantitative analysis through principal component analysis and partial least-squares regression. The SERS substrates adopted in this application were synthesized by in situ growth of silver nanoparticles on diatomaceous earth films, which show ultra-high sensitivity down to 10 parts per trillion in artificially contaminated tap water in the lab using a commercial portable Raman spectrometer. Based on training data from artificially contaminated tap water, we predicted the fentanyl concentration in the sewage water from a wastewater treatment plant to be 0.8 parts per billion (ppb). As a comparison, the HPLC-MS confirmed the fentanyl concentration was below 1 ppb but failed to provide a specific value of the concentration since the concentration was too low. In addition, we further proved the validity of our SERS sensing technique by comparing SERS results from multiple sewage water treatment plants, and the results are consistent with the public health data from our local health authority. Such SERS sensing technique with ultra-high sensitivity down to sub-ppb level proved its feasibility for point-of-care detection of illicit drugs from sewage water, which is crucial to assess public health.

An ultrasensitive surface-enhanced Raman scattering sensor for the detection of hydrazine via the Schiff base reaction

The development of convenient assays for the determination of hydrazine (N2H4) has drawn significant attention due to the high toxicity of this substance. Herein, we developed a concise, rapid and ultrasensitive surface-enhanced Raman scattering (SERS) sensor for N2H4 detection based on alpha-cyclodextrin-silver nanoparticles (α-CD-AgNPs) modified by 4-mercaptobenzaldehyde (4-MBA). The 4-MBA molecules can specifically capture the N2H4 molecules and undergo a Schiff base reaction. As a result, this induces the aggregation of nanoparticles and generates a new characteristic peak at 1529 cm–1 that is attributed to CN and CC vibrations. Compared with noble metal nanoparticles, 4-MBA not only formed AgS bonds but could also be fixed in the cavity of cyclodextrin to produce a more stable and stronger SERS signal. The SERS intensity at 1529 cm–1 and the logarithm of the concentration of N2H4 presented a good linear relationship from 10–9 to 10–7 M with an unprecedented limit of detection (LOD) of 38 pM. The proposed SERS sensor exhibited satisfactory selectivity and reproducibility and was applied to detect N2H4 in real and complex water samples. We expect this assay to be a promising alternative tool for the on-site detection of N2H4.

In Situ Monitoring of Hydrogen Peroxide Released from Living Cells Using a ZIF-8-Based Surface-Enhanced Raman Scattering Sensor.

Hydrogen peroxide (H2O2) widely involves in intracellular and intercellular redox signaling pathways, playing a vital role in regulating various physiological events. Nevertheless, current analytical methods for the H2O2 assay are often hindered by relatively long response time, low sensitivity, or self-interference. Herein, a zeolitic imidazolate framework-8 (ZIF-8)-based surface-enhanced Raman scattering (SERS) sensor has been developed to detect H2O2 released from living cells by depositing ZIF-8 over SERS active gold nanoparticles (AuNPs) grafted with H2O2-responsive probe molecules, 2-mercaptohydroquinone. Combining the superior fingerprint identification of SERS and the highly efficient enrichment and selective response of H2O2 by ZIF, the ZIF-8-based SERS sensor exhibits a high anti-interference ability for H2O2 detection, with a limit of detection as low as 0.357 nM. Satisfyingly, owing to the enhanced catalytic activity derived from the successful integration of AuNPs and ZIF, the response time as short as 1 min can be obtained, demonstrating the effectiveness of the SERS sensor for rapid H2O2 detection. Furthermore, the developed SERS sensor enables real-time detection of H2O2 secreted from living cells under phorbol myristate acetate stimulation, as cells can be cultured on-chip. This study will pave the way toward the development of a metal-organic framework-based SERS platform for application in the fields of biosensing and early disease diagnosis associated with H2O2 secretion, thus exhibiting promising potential for future therapies.

Recent Advances Towards Point-Of-Care Applications of Surface-Enhanced Raman Scattering Sensing.

The ability to accurately diagnose at the point of care is crucial in many pathologies. However, current standard diagnostic practices can only be performed in specialized health or laboratory settings. To move diagnostic methods from a specialized lab to the point of care many alternate methods have been developed and proposed. Among them surface-enhanced Raman scattering (SERS) sensing offers advantageous features, such as simultaneous detection of multiple biotargets and increased accuracy. Many groups have been working towards the translation of SERS sensing methods from the lab to the point of need. In this mini review, we discuss interesting and recent developments in this effort, focusing on how different sensing mechanism can be used in point-of-care testing applications of SERS.

An all-in-one magnetic SERS nanosensor for ratiometric detection of Escherichia coli in foods.

An all-in-one nanosensor was developed for the magnetic enrichment and ratiometric surface-enhanced Raman scattering (SERS) detection of Escherichia coli (E. coli). The all-in-one nanosensor was constructed through the chemical integration of four components into a single nanoparticle, which include a manganese ferrite nanoparticle serving as the magnetic core, a thin silver shell as the SERS substrate, a self-assembled layer of 4-mercaptobenzoic acid (MBA) molecules as the SERS internal standard, and a MBA-conjugated layer of aptamer sequences as the capture probe of E. coli. In the detection of E. coli in food, the target cells were first captured by the nanosensors and magnetically enriched in a short time of 15 min, and then the ratiometric SERS was performed through the Raman intensity ratio between two specific SERS peaks produced by the captured E. coli and the internal MBA. The pre-concentration and ratiometry enabled the nanosensor to detect E. coli with a detection limit down to 10 CFU/mL. The all-in-one nanosensor was successfully applied for the detection of E. coli in various liquid foods including milk, juice, tea, and coffee, with recoveries ranging from 89 to 110% and relative standard deviation lower than 1.7%. In comparison with the previous sandwich strategy adopted by most SERS sensors, this nanosensor endowed with an easier use and a lower cost is more sensitive and reproducible, leading to a great potential in practical applications.

Sensing Performance of Mono and Bimetallic Nano Photonics Surface Enhanced Raman Scattering (SERS) Devices

In this research, sensing performance of mono and bimetallic nanophotonics SERS sensors of gold-silver nano-columns for the detection of chlorpyrifos was investigated. For optimum substrates for Gold-silver/nano-column surface-enhanced, Raman scattering (SERS) was achieved with the silicon substrate. By combining the Ag SERS activity with the Au chemical stability and nano-columns Si large field enhancement, the Au-Ag/nano-columns Si substrate revealed perfect reproducibility, homogeneity, sensitivity in addition to chemical stability. The sensors were tested by Atomic force microscope (AFM), field emission scanning electron microscope (FESEM), energy-dispersive X-ray analysis (EDS), and X-ray diffraction (XRD). Findings presented in this research indicated modified distributions and sizes of formed alloy nanoparticles, and the hot spots junctions within the nanophotonics layer after changing the nanoparticles types. The SERS sensors performance displayed an excellent recognition of ultra-low concentrations of chlorpyrifos solutions with an exponential relationship with the Raman signal. The highest enhancement factor (Ef=1.56×106) and minimum limit of detection 0.069 mg/Kg were obtained with Au-Ag sensors.

Determination of Dichlorvos in Pears by Surface-Enhanced Raman Scattering (SERS) with Catalysis by Platinum Coated Gold Nanoparticles

A surface-enhanced Raman scattering (SERS) sensor is reported for the determination of dichlorvos, an organophosphate pesticide (OP), in pears. Platinum coated gold nanoparticles (Au@PtNPs) were used as secondary catalysts to provide signal amplification. Acetylcholinesterase (AChE) catalyzed the hydrolysis of acetylthiocholine to form thiocholine. Thiocholine causes the accumulation of Au@PtNPs, which changes the particle size and prevents their peroxidase-mimicking activity. Dynamic light scattering was used to characterize the particle size. Following the addition of dichlorvos, the AChE activity was inhibited, the production of thiocholine and aggregation were reduced, and the peroxidase mimicing property was retained. In the presence of 3,3′,5,5′-tetramethylbenzidine (TMB), a blue product was produced that provided strong SERS signals. The SERS method showed a linear relationship for dichlorvos concentrations from 20 to 2000 µg/L. The developed protocol had a limit of detection of 20 µg/L. This SERS sensor assay was successfully applied for the quantification of dichlorvos in pears with satisfactory recoveries from 80.25 to 94.86%.

(Invited) Resonator and SERS Sensing in the Field of Drug Delivery

Introduction Our research center of excellence IDUN combines research in nanosensors/centrifugal microfluidics and microfabricated devices for oral drug delivery. This allows us to explore the synergy between sensor development and search for new pharmaceutical delivery tools and materials. Our developed nanosensors get access to unique polymers and biomolecules and are able to characterize, among others, small volumes of materials and molecules, which are today not possible to analyze by any standard technologies. We will show examples of recent findings and results within drug/polymer characterization (1) and process monitoring (2) using nanomechanical sensors. Also, new applications within therapeutic drug monitoring using Surface Enhanced Raman Scattering (SERS) will be presented as well as sensor integration with centrifugal microfluidics platforms. Resonators By monitoring the resonance frequency shift of micrometer sized strings or cantilevers coated with thin layers of polymers, it is possible to monitor phase transitions using only ng of material. We have been able to observe beta relaxation of polymers (3) and drugs (2), see figure 1. These systems also allowed us to monitor degradation of biomaterials used in the fabrication of the microcontainers (4). Also, strings are inherently sensitive to temperature changes (5). The resonant frequency detunes as a function of temperature and temperature changes down to 10-5 °C can be detected. The effect has been used to perform photothermal IR spectra on samples deposited on resonating strings and resonating filters. This has been used to for example study the amorphous and crystalline forms of indomethacin (1). As an alternative to cleanroom fabricated resonators we have used single drug and collagen particles as micro mechanical resonators, thereby eliminating the need for cleanroom processing. This method additionally makes it possible to study the behavior of single crystals, which would otherwise be extremely difficult/impossible. This method has, among others, allowed us to study new phenomena in the hydration/dehydration of drugs (6). Centrifugal microfluidics The optics and mechanics from a DVD player can be used to realize compact and sensitive sensor systems. By rotating a polymer disc with integrated microfluidic channels it is possible to manipulate liquid samples such as blood – performing crucial operations like separation, valving and mixing. We integrate sensor elements such as cantilevers, nanoparticles, resonating strings and SERS substrates with centrifugal microfluidics (7). The sensors are read out by a DVD pick-up head, which can perform transmission/absorption measurements and can detect nm deflections. Also, electrodes and spectrometers are integrated on a disc platform, facilitating electrochemical/Raman measurements. Our nanopillar SERS substrate can be used for sample filtration and separation. Here the nanopillars function similarly to a filter paper in chromatography and can be used to distinguish e.g. different components in a urine sample as well as drug residuals. A SERS chip is placed in a centrifugal microfluidics platform, whereby the sample can be initially centrifuged. Subsequently half the SERS chip is exposed to the centrifuged liquid. Hereby, liquid can travel up the chip by the so-called wicking effect, and depending on affinity of molecules to the surface, different molecules will travel different lengths. Hereby, a separation is secured and the different molecules are read-out as bands on the SERS chip. Acknowledgements This research has received funding from the ERC grant agreement n° 320535 and from the Center of Excellence ’IDUN’ granted by Danish National Research Foundation, DNRF122/the Velux Foundations.

Sensitive, Reusable, Surface-Enhanced Raman Scattering Sensors Constructed with a 3D Graphene/Si Hybrid.

Surface-enhanced Raman scattering (SERS) substrates based on graphene and its derivatives have recently attracted attention among those interested in the detection of trace molecules; however, these substrates generally show poor uniformity, an unsatisfactory enhancement factor, and require a complex fabrication process. Herein, we design and fabricate three-dimensional (3D) graphene/silicon (3D-Gr/Si) heterojunction SERS substrates to detect various types of molecules. Notably, the detection limit of 3D-Gr/Si can reach 10-10 M for rhodamine 6G (R6G) and rhodamine B (RB), 10-7 M for crystal violet (CRV), copper(II) phthalocyanine (CuPc), and methylene blue (MB), 10-8 M for dopamine (DA), 10-6 M for bovine serum albumin (BSA), and 10-5 M for melamine (Mel), which is superior to most reported graphene-based SERS substrates. Besides, the proposed 3D-Gr/Si heterojunction SERS substrates can achieve a high uniformity with relative standard deviations (RSDs) of less than 5%. Moreover, the 3D-Gr/Si SERS substrates are reusable after washing with ethyl alcohol to remove the adsorbed molecules. These excellent SERS performances are attributed to the novel 3D structure and abundantly exposed atomically thin edges, which facilitate charge transfer between 3D-Gr and probe molecules. We believe that the 3D-Gr/Si heterojunction SERS substrates offer potential for practical applications in biochemical molecule detection and provide insight into the design of high-performance SERS substrates.

Gold Nanomaterials as a Promising Integrated Tool for Diagnosis and Treatment of Pathogenic Infections—A Review

This review summarizes research on functionalized gold nanomaterials as pathogen detection sensors and pathogen elimination integrated tools. After presenting the challenge of current severe threat from pathogenic bacteria and the increasingly serious growth rate of drug resistance, the first section mainly introduces the conspectus of gold nanostructures from synthesis, characterization, physicochemical properties and applications of gold nanomaterials. The next section deals with gold nanomaterials-based pathogen detection sensors such as colorimetric sensors, fluorescence sensors and Surface-Enhanced Raman Scattering sensors. We then discuss strategies based on gold nanomaterials for eliminating pathogenic infections, such as the dual sterilization strategy for grafting gold nanomaterials with antibacterial substances, photothermal antibacterial and photodynamic antibacterial methods. The fourth part briefly introduces the comprehensive strategy for diagnosis and sterilization of pathogen infection based on gold nanomaterials, such as the diagnosis and treatment strategy for pathogen infection using Roman signals real-time monitoring and photothermal sterilization. A concluding section that summarizes the current status and challenges of the novel diagnosis and treatment integrated strategy for pathogenic infections, gives an outlook on potential future perspectives.

Surface-enhanced Raman scattering sensors for biomedical and molecular detection applications in space.

The detection of molecular traces in the environment is a technical problem that is critical in pollutant control procedures at all stages of spacecraft assembly, in space flight, as well as in other technological processes such as food production, medical diagnostics, environmental control, warfare. However, in the aerospace industry, it is necessary to detect molecular traces of contaminants with extreme sensitivity, as even concentrations as low as part-per-billion (ppb) can be critical during long missions. The high sensitivity of the Volatile Organic Compounds (VOCs) detection within the air can be a challenge because of the poor affinity of VOC’s to the metal surface of the sensor substrate. In this work, we present a surface‐enhanced Raman scattering (SERS) spectroscopy technique as a highly sensitive and selective molecular sensor for gas trace detection not sensitive to molecules adsorbtion on sensing element. The developed hybrid SERS platform for molecular trace detection is supported by the hybrid nanoplasmonic porous silicon membrane in conjunction with micropump to achieve the trace level detection of VOCs in the environment. The combination of silicon membrane, made by electrochemical etching of the microchannels in the silicon chip, with chemical deposition of the silver nanoparticles inside the channels, produce a porous Ag nanoparticles membrane with a high density of plasmonic nanostructures (“hot spots”). The micropump integrated with the SERS sensor, pump the air with VOC’s molecules through the plasmonic membrane “hot spots” to increase the probability of interaction of VOC’s molecules with SERS substrate and to increase the enhancement factor. The sensor chip structure was designed, gas flow in the sensor was simulated, and the sensor was fabricated using 3D printing. The limit of detection of hydrazine with concentration level 10–12 M from solution and the vapor phase 0.1 ppm was demonstrated. The anisole vapors with concentration 0.5 ppb spectra in the air were recorded. Our results demonstrate that plasmonic membrane can be used as a high enhancement factor SERS sensor for many pollutants molecules detection with the nanomolar sensitivity and can be applied in the design of sensors for space applications, environment control, biomedical diagnostic.Supplementary InformationThe online version contains supplementary material available at 10.1007/s12567-021-00356-6.

Surface-enhanced Raman scattering effect in binary systems formed by graphene on aluminum plasmonic nanostructures

Binary systems (BS) formed by graphene (GR) deposited on top of aluminum (Al) nanoconcaves (Al-NC) and Al nanodomes (Al-ND) were synthesized by electrochemical anodization of Al. Using the plasmonic response of Al-NC and Al-ND and the distinctive physical and chemical properties of GR, these BS are proposed as Surface-Enhanced Raman Scattering (SERS) sensors using rhodamine 6G (R6G) as a proof molecule. As expected, the BS significantly enhances Raman signals of R6G molecules in comparison with substrates used as references, also suppressing the fluorescence background of R6G molecules.

One-Pot Synthesis of a Three-Dimensional Au-Decorated Cellulose Nanocomposite as a Surface-Enhanced Raman Scattering Sensor for Selective Detection and in Situ Monitoring

Metal nanoparticles (NPs) have been commonly introduced onto flexible platforms for improving their exploitation. However, such an introducing process of NPs is hard to achieve, and additional dispersants and high-energy consumption are largely required in existing studies. In this study, a one-pot method was developed to synthesize Au NPs in a cellulose dope. The dissolved cellulose chains acted as a green reductant as well as a stabilizer. As the polysaccharide dope coagulated, a three-dimensional (3D) regenerated cellulose nanocomposite decorated with 0.29–1.07 wt % Au NPs was successfully synthesized. As supported by the “hot spot” effect among the Au NPs embedded in the 3D structure, the nanocomposite could act as a sensitive surface-enhanced Raman scattering (SERS) substrate. Accordingly, this study achieved an enhancement factor of 2.8 × 107 and a limit of detection of 10–9 M when R6G was employed as a probe molecule. Moreover, as impacted by the porous structure of the SERS substrate, 2.5 mg/kg melamine in milk could be directly detected. Furthermore, organic contaminants were catalytically reduced, and the process of catalytic reduction underwent in situ SERS monitoring with excellent sensitivity.

In situ photochemical deposition of Ag nanoparticles on polyester fiber membranes as flexible SERS substrates for sensitive detection of sodium saccharin in soft drinks

Flexible surface-enhanced Raman scattering (SERS) substrates, specifically paper-based SERS substrates, are generally employed in many practical applications. However, paper-based SERS substrate quantitation is still challenging because of the inferior uniformity originating from the irregular morphology of the paper. The present study synthesized Ag nanoparticles (NPs) via the in situ photochemical reduction of Ag+ ions on a titanium dioxide (TiO2) film at the polyester fiber membranes (PFMs) surface. This generated many “hot spots” that presented significant SERS activity with an enhancement factor of 2.1 × 109. Sodium saccharin (SS) bonded to the Ag NPs surface via the carbonyl oxygen and ring nitrogen atoms. A SERS sensor with photochemically deposited Ag NPs was prepared for the sensitive and rapid detection of SS in soft drinks with a limit of detection of 0.3 mg/L. The recovery rate ranged from 93% to 103%, indicating that this method is reliable in actual sample detection with simple pretreatment steps and can be potentially applied for the on-site detection of food additives for food safety.

Role of polyelectrolyte multilayers over gold film for selective creatinine detection using Raman spectroscopy

Development of nano-roughened surface-enhanced Raman scattering (SERS) substrates is still of great interest for applications in SERS sensor with good sensitivity, selectivity, and uniformity. In this work, nanostructured polyelectrolyte films coated Au film is designed as a SERS substrate for label-free and selective creatinine detection. Two-component multilayer films are fabricated by layer-by-layer (LbL) deposition of polyacrylic acid (PAA) and polyallylamine hydrochloride (PAH). The SERS sensing behaviors including electric field enhancement, sensitivity, and selectivity strongly influence by morphology and types of polyelectrolyte. The dense polyelectrolyte nanostructured film over Au substrate (30 PAA/PAH bilayers) possesses the strongest SERS hotspots. The PAH outermost layer exhibits the highest creatinine signal comparing to other selected polyelectrolytes. This creatinine signal could amplify by free vibration of creatinine on PAH. This substrate exhibited good performance for creatinine detection in pH 5 to 7-buffered solutions. By performing creatinine analysis in artificial urine, good sensitivity with wide linear response (1 µM to 10 mM) and low detection limit of 1.47 µM was found. Moreover, this substrate has high selectivity for creatinine detection compared to interferences. With the contribution of both plasmonic Au film and polyelectrolyte multilayers, these findings underscore the guideline for creating selective SERS substrate for creatinine detection.

Surface-Enhanced Raman Scattering Sensing of Transition Metal Ions in Waters.

In this mini-review, we provide a coherent discussion on the sensing schemes exploited in the surface-enhanced Raman scattering (SERS) analysis of transition metal ions in waters. A critical approach was used where illustrative examples are selected to discuss key drawbacks and challenges associated with various experimental configurations and the employed enhancing substrates.

Intracellular pH – Advantages and pitfalls of surface-enhanced Raman scattering and fluorescence microscopy – A review

The value of pH in various parts of protoplasm can affect nearly all aspects of cell functions. Therefore, the determination of intracellular acid-base features is required in many areas of biological and biochemical studies. Because of a significant scientific importance of in vivo intracellular pH measurements, various groups carried out such experiments. In this review article we describe intracellular pH measurements using two the most sensitive optical spectroscopies: surface-enhanced Raman scattering (SERS) and fluorescence. It is reasonable to present these two techniques in one review article because the experimental approach in Raman and fluorescence experiments is relatively similar. The basic theoretical background explaining the mechanism of operation of fluorescence and SERS sensors are discussed and the motivations to carry out intracellular pH measurements are briefly described. Future perspectives in this field are also discussed.

Large-Scale Flexible Surface-Enhanced Raman Scattering (SERS) Sensors with High Stability and Signal Homogeneity

Flexible surface-enhanced Raman scattering (SERS) sensors have attracted great attention as a portable and low-cost technique for chemical and bio-detecting. However, flexible SERS sensors tend to suffer low signal spatial homogeneity due to their uneven active plasmonic nanostructures (hot-spots) distribution, and quick degradation of SERS sensors' sensitivity due to low adhesion of hot-spots and flexible substrates during fast sampling. Herein, a large area (20×20 cm2) polyimide (PI) based SERS sensor is exploited for trace detection with high signal homogeneity and stability. The SERS sensor is constructed from PI through in situ growth of silver and gold core-shell nanoparticles (Ag@Au NPs) based on chemical reduction and galvanic replacement process. Benefiting from the abundant carboxyl groups on the surface cleavaged PI, densely and uniformly distributed Ag@Au NPs are successfully prepared on the film under ambient conditions. The high Raman enhancement factor (up to 1.07 × 107) and down detection capability of nanomolar (10-9 M) detection limits are obtained for this flexible SERS sensor. The uniform Raman signals in the random region show well signal homogeneity with a low variation of 8.7%. Moreover, the flexible SERS sensor exhibited superior efficiency durability after storage 30 days even after mechanical stimuli (bending or torsion) 500 cycles. The pesticide thiram (TMTD) residue has been rapidly traced by direct sampling from the apple surface, and a sensitivity of 10 ng/cm2 for TMTD was achieved. These findings show that the PI-based SERS sensor is a very strong candidate for broad and simple utilization of flexible SERS for both laboratory and commercial applications in chemical and biomolecule detections.

PCR-coupled Paper-based Surface-enhanced Raman Scattering (SERS) Sensor for Rapid and Sensitive Detection of Respiratory Bacterial DNA

A paper-based surface-enhanced Raman scattering (SERS) substrate composed of silver-nanowires (AgNWs) was coupled with polymerase chain reaction (PCR) for rapid and sensitive determination of respiratory bacterial DNA. EvaGreen dye, which is a DNA intercalating molecule, was introduced to the low-cycled PCR product, and the difference in dye intercalation in the DNA structure was quantified by Raman spectroscopy to verify the presence of a target gene. Mycoplasma pneumoniae (M. Pne), was selected as a target for the sensing demonstration. At various gene concentrations and amplification cycles, detection capability of fluorescence-based real-time PCR (RT-PCR) and the PCR-coupled SERS method were compared to develop a DNA detection system capable of low-cycle amplification and sensitive DNA sensing. After 10 cycles, the PCR-coupled SERS method showed enhanced detection capability with a DNA detection limit of 3.12 pg/μL. Then, the SERS substrate was prepared as a rapid kit that included a test line and negative and positive control lines. Target DNA after 10 cycles of amplification was successfully discriminated compared to non-target DNA and it was statistically relevant. The developed system is expected to be used as a detection method for various bacteria and viruses, and also be integrated with diverse POC diagnostic systems.