The present experimental study aims at investigating the impact of adding graphene oxide nanoparticles (GO) to neat Jatropha Methyl Ester (JME) on a single cylinder air cooled direct injection four stroke diesel engine. The nano-fuels have been prepared from 25, 50, 75 and 100 mg/l concentrations of graphene oxide with neat Jatropha biodiesel through ultrasonication process. The graphene oxide nanoparticles crystallite size, morphology, and the chemical structures were examined using X-ray diffraction (XRD), Transmission Electron Microscope (TEM), and Fourier-transform infrared spectroscopy (FTIR), respectively. The compression ignition engine characteristics were investigated by the four JME-GO blends, and their results were compared with neat JME under various engine loads at a constant engine speed of 2000 rpm. The results indicate that the diesel engine operated by JME-GO nano-fuels enhanced the brake thermal efficiency by 17% compared to neat JME fuel. Furthermore, the peak cylinder pressure, the highest rate of pressure rise, and maximum heat release rate were also increased by 8%, 6%, and 6%, respectively. The CO and UHC emissions were decreased significantly by 60% and 50%, respectively, for JME-GO blends compared to pure JME fuel. At high engine load, the NOx emission was reduced by 15% for JME-GO blends compared to pure Jatropha biodiesel. The results also illustrated that the concentration of 50 mg/L had the optimum improvement in the overall characteristics of engine performance and emissions.