Abstract Objective: Arterial stiffness (AS) is a prominent risk factor for cardiovascular diseases. It is known that different polymorphisms are associated with cardiovascular diseases, while there is insufficient evidence that genetic traits lead to arterial wall changes, including AS. The aim of this study was to find the association between increased AS and polymorphisms that are associated with cardiovascular diseases and are involved in renin-angiotensin-aldosterone system, chronic inflammation and oxidative stress in healthy adults. Design and method: We investigated 153 healthy adults, mean age 50.56 ± 13.14 years, 35% male, 22% smokers. Increased AS was defined as pulse wave velocity (PWV) > 10 m/s. Measurements were performed by applanation tonometry using SphygmoCor device (AtCor, Australia). Insulin resistance was diagnosed if HOMA IR was > 2,5. Leucocyte telomere length (LTL) were measured by real-time PCR. AGT, ACE, NOS3, TNF, MMP9 polymorphisms were detected by real-time PCR using DNA technologies (Russia) standard kits, and CYBA polymorphism was detected by Applied Biosystems (USA) standard kit. Associations of genotypes and alleles with increased PWV were studied using chi-square or Fisher's exact test. Multiple logistic regression models were created to identify the effect of genotype and risk factors on PWV. Results: Increased AS was associated with D-allele (OR = 1.89, 95% CI: 1.16–3.12, p = 0.014) and DD-genotype (OR = 3.42, 95% CI: 1.39–9.36, p = 0.005) of ACE Ins > Del polymorphism. ACE Ins > Del polymorphism predicted increased AS along with sex, age and LTL. The results of multiple logistic regression analysis are represented in the table. Conclusions: In healthy adults increased AS is strongly associated with DD-genotype of ACE polymorphism, sex, age and leucocyte telomere length