Ganoderma Lucidum Strain Research Articles

Breeding a new Ganoderma lucidum strain with increased contents of individual ganoderic acids by mono-mono crossing of genetically modified monokaryons.

Ganoderic acids (GAs) are major functional components of Ganoderma lucidum. The study aimed to breed a new G. lucidum strain with increased contents of individual GAs. Two mating-compatible monokaryotic strains, G. 260125 and G. 260124, were successfully isolated from the dikaryotic G. lucidum CGMCC 5.0026 via protoplast formation and regeneration. The Vitreoscilla hemoglobin gene (vgb) and squalene synthase gene (sqs) were overexpressed in the monokaryotic G. 260124 and G. 260125 strain, respectively. Mating between the G. 260124 strain overexpressing vgb and the G. 260125 strain overexpressing sqs resulted in the formation of the new hybrid dikaryotic G. lucidum strain sqs-vgb. The maximum contents of ganoderic acid (GA)-T, GA-Me, and GA-P in the fruiting body of the mated sqs-vgb strain were 23.1, 15.3, and 39.8 μg/g dry weight (DW), respectively, 2.23-, 1.75-, and 2.69-fold greater than those in G. lucidum 5.0026. The squalene and lanosterol contents increased 2.35- and 1.75-fold, respectively, in the fruiting body of the mated sqs-vgb strain compared with those in the G. lucidum 5.0026. In addition, the maximum expression levels of the sqs and lanosterol synthase gene (ls) were increased 3.23- and 2.13-fold, respectively, in the mated sqs-vgb strain. In summary, we developed a new G. lucidum strain with higher contents of individual GAs in the fruiting body by integrating genetic engineering and mono-mono crossing.

Whole-Genome Sequencing and Transcriptome Analysis of Ganoderma lucidum Strain Yw-1-5 Provides New Insights into the Enhanced Effect of Tween80 on Exopolysaccharide Production.

Ganoderma lucidum is an important medicinal mushroom widely cultured in Asian countries. Exopolysaccharides are bioactive compounds of G. lucidum with health benefits. Limited exopolysaccharide content hinders its extraction from G. lucidum. The addition of Tween80 had an enhanced effect on G. lucidum exopolysaccharide production in submerged fermentation. However, the mechanism of this effect remains unclear. In this study, we report on a high-quality assembly of G. lucidum strain yw-1-5 to lay the foundation for further transcriptome analysis. The genome sequence was 58.16 Mb and consisted of 58 scaffolds with an N50 of 4.78 Mb. A total of 13,957 protein-coding genes were annotated and Hi-C data mapped to 12 pseudo-chromosomes. Genes encoding glycosyltransferases and glycoside hydrolases were also obtained. Furthermore, RNA-seq was performed in a Tween80-treated group and control group for revealing the enhanced effect of Tween80 on exopolysaccharide production. In total, 655 genes were identified as differentially expressed, including 341 up-regulated and 314 down-regulated. Further analysis of differentially expressed genes showed that groups of MAPK, amino sugar and nucleotide sugar metabolism, autophagy, ubiquitin-mediated proteolysis, peroxisome, starch and sucrose metabolism, TCA cycle, glycolysis/gluconeogenesis KEGG pathway, glycosyltransferases and glycoside hydrolases played important roles in the enhanced effect of Tween80 on exopolysaccharide production. This work provides a valuable resource for facilitating our understanding of the synthesis of polysaccharides and accelerating the breeding of new strains with a high content of exopolysaccharides.

Phorbol ester biodegradation in Jatropha curcas cake and potential as a substrate for enzyme and Pleurotus pulmonarius edible mushroom production

Jatropha curcas is a model for biorefinery applications. Although J. curcas cake (JCC), a product of oil extraction, shows potential in animal feed, inactivation of toxic phorbol esters (PE) in residues is necessary. Biological detoxification offers promise for animal feed, as well as for edible mushroom and enzyme production. Here, strains of Aurantioporus pulcherrimus, Ganoderma lucidum, Agaricus sp., Agaricus fuscofibrillosus, Agaricus mediofuscus, Ascopolyporus sp., Panaeolus antillarum, Lentinus strigellus, Amylosporus sp. and Pleurotus pulmonarius were screened for degradation of PE in JCC. These selected macrofungi degraded PE in solid culture over a 30-day period at 28 °C. Among the evaluated basidiomycetes, P. pulmonarius displayed the highest rate of PE degradation, reaching 97% efficiency. To induce the formation of fruiting bodies, P. pulmonarius was further cultivated over 60 days in JCC and five different JCC formulations, each supplemented with an additional lignocellulosic source, with up to a 99.5% degradation of the toxic compound achieved. Fungal fruiting was observed in four of the formulations tested. Biological efficiency (BE) reached 25% in a formulation of JCC plus sawdust. Ligninases, cellulases, hemicellulases, proteases, phytases, esterases and lipase activities were quantified from crude extracts of Spent Mushroom Substrate (SMS) from this formulation. Bromatological analyses here also revealed increases in protein content, Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), cellulose and hemicellulose, and a reduction in dry matter, lignin, and ethereal extract. In conclusion, P. pulmonarius was efficient in degradation of PE in JCC, with the residue appropriate for animal feed input, for production of edible mushrooms, and as a source of enzymes of biotechnological interest.

Hydrodynamically Activated Sawdust of Common Pine Pinus sylvestris L.-substrate for Cultivation of the Strain Gl4–16A Ganoderma lucidum

The results of a study on the use of sawdust of common pine Pinus sylvestris L. with their preliminary activation in a cavitation water-impact dispersant for 25 minutes as a substrate for cultivating the Gl4–16A strain of Ganoderma lucidum are presented. It was found that the Gl4–16A strain of Ganoderma lucidum colonizes the substrate more efficiently with the introduction of (NH4)2SO4 and Na2HPO4 salts into its composition. At the same time, the average growth rate of basidiomycete on the substrate without the introduction of salts was 1.39±0.53 mm/day, and on the substrate with nitrogen salts – 3.22±0.48 mm/day, and complete fouling of the substrate occurred on day 13. It was found that during the cultivation of the Gl4–16A strain of Ganoderma lucidum on a substrate with nitrogen salts, the content of extractive substances in it increases from 4.37 % to 6.32 %, where more than 65 % is accounted for by substances extracted with water. There is a decrease in the content of difficult-to-hydrolyze polysaccharides by 13.3 %, and an increase in the non-hydrolyzable part by 2.4 %, which is 6.8 % per organic mass. The content of easily hydrolyzable polysaccharides (LGA) decreases by 2.4 %, but this change is not significant in terms of organic mass and the share of LGA both before and after bioconversion is about 15.7 %. The obtained data can expand the field of use of sawdust, which is a promising direction, both in the chemical and pharmaceutical industries

Optimization of Growth Conditions and Biological Activities of Nepalese Ganoderma lucidum Strain Philippine.

Ganoderma lucidum has been extensively studied for its valuable medicinal importance. In this study, the artificial cultivation of G. lucidum strain Philippine in different culture media, including sawdust substrate, was performed and optimized on the Potato Dextrose Agar (PDA) media. Phytochemical, antibacterial, and antioxidant analyses were performed and compared between the ethanol extracts prepared from two different cultures (fruit from synthetic log culture and mycelia from PDA media culture). Both the 200 mg/mL and 100 mg/mL concentrations of extracts inhibited all the tested bacteria, and the results were promising than the corresponding control using antibiotics. The fruit extract showed higher antioxidant potential (150.6 ± 56.92 mg ascorbic acid equivalent/g extract) than mycelial extract (144.28 ± 81.72 mg ascorbic acid equivalent/g extract). The results indicate that fruiting bodies of G. lucidum cultivated in a complex dust medium possess higher antioxidant properties than mycelia culture, which can be further explored for therapeutic applications.

Development of efficient strain of Ganoderma lucidum for biological stripping of cotton fabric dyed Reactive Blue 21

One of the most common dyeing problems of textile industries is uneven and faulty dyeing over the finished quality of fabrics due to different reasons. These problems are usually tackled through chemical degradation in which uneven and faulty dye is removed from the surface of fiber but fabric quality is compromised. Chemical process also reduces the strength of the fabric and durability of textile material by reduction in reactive dye ability. The fabric cannot be reused due to the reduced strength. To overcome above mentioned problem, biological method of stripping in which enzymes produced by different micro-organisms are used. This process has no harmful effect on the fabric and is safe for environment. In this research work reactive blue 21 dye with 0.5, 2 and 4% shade strengths was used to dye cotton fabric. The Ganoderma lucidum fungal strains were mutated by UV mutagen, and five were selected for further processing. These mutant strains were grown at temperature ranges (20 °C to 40 °C); pH(3–5); inoculum size(1–5 mL) and fermentation time (3–15 days) . The required nutrients media to produce the ligninolytic enzymes was added to the flask. The strain which gave the fast decolourization results was selected for further optimization. Optimization was done by observing the variables: incubation time 12 days, pH 4, temperature 30 °C, and inoculum size 3 mL by applying Response Surface Methodology (RSM) in Central Composite Design (CCD). During the process of fabric color stripping, the enzyme assay revealed that the respective mutant UV-60 strain produced active enzymes with their Vmax, Mnp (427U/mL), LiP (785U/mL), and Lac (75 U/mL) enzymes decolorized 89% of the dye which is 25% more than the parent strain and also the production of enzyme is Mnp (344U/mL), LiP (693U/mL), and Lac (59 U/mL) enzymes which is lower than mutant strain.

Prospects for using Ganoderma lucidum (Curtis) P. Karst. for biological control of phytopathogenic fungi

Antifungal, morphological and cultural properties of the Siberian and Abkhaz strains of the medicinal basidiomycete Ganoderma lucidum (Curtis) P. Karst were studied. A group of strains characterized by high growth rates in vitro on agar media (7.3-8.0 mm / day) and plant waste from timber processing (3.3-3.9 mm / day) was found. All strains of G. lucidum exhibited antifungal activity against phytopathogenic fungi; the degree of phytopathogen inhibition ranged from 10 to 58 %. Basidial rootrot fungi Heterobasidion and ascomycete fungi Bipolaris, Alternaria, Fusarium are the most sensitive to the presence of G. lucidum. The cultures most effectively limiting the phytopathogen development in the group of fast-growing Abkhaz strains (antifungal activity ranged from 21 to 58%) were identified. The high antifungal activity of strains on lignocellulosic substrates allows us to consider them as promising biocontrol agents for reducing the number, primarily, of basidial phytopathogens.

Potential of waste reduction of agro-biomasses through Reishi medicinal mushroom (Ganoderma lucidum) production using different substrates and techniques

One of the most important priorities in the medicinal mushrooms cultivation is the selection and preparation of the substrate, which depends on factors such as price and easy access to different lignocellulosic compounds. In order to investigate the effect of mixed and non-mixed agro-waste substrates on some vegetative, reproductive, nutritional and medicinal properties of two Iranian and Chinese strains of Ganoderma lucidum, the factorial experiment was conducted as a completely randomized design with three replications. The experimental substrates of different poplar wood chip (PWC), wheat bran (WB), soybean meal (SM), date palm leaf (DPL) and olive oil extraction waste (OOEW) were used. They were enriched with chemical supplements (ammonium nitrate, manganese sulfate and Nano manganese oxide) using two cultivation techniques (open bag door and Polika-Cotton) for fruiting body production.The results showed that various substrates significantly affect dry weight, moisture content quantity, vegetative and fruit yield, total ganoderic acids content, total polysaccharide value and mineral elements amount (nitrogen, potassium and calcium) in fruiting body. The highest amount of N content in the fruiting body of both Iranian and Chinese strains (5.88 and 6.25 mg/100 g dry matter) was dedicated to the combined PWC + SM (70:30) substrate enriched with Nano manganese oxide supplement and Polika-Cotton method. The highest total fresh of Iranian and Chinese strains weight (109.60 and 127.66 g, respectively) obtained in the combined PWC + WB (70:30) substrate enriched with manganese sulfate. The highest amount of total ganoderic acids in fruiting organs in both strains (0.52 and 0.42 μg/mg dry matter) is achieved using a combined PWC + SM (70:30) enriched with manganese sulfate.

Integrative Analysis of Selected Metabolites and the Fungal Transcriptome during the Developmental Cycle of Ganoderma lucidum Strain G0119 Correlates Lignocellulose Degradation with Carbohydrate and Triterpenoid Metabolism.

To systemically understand the biosynthetic pathways of bioactive substances, including triterpenoids and polysaccharides, in Ganoderma lucidum, the correlation between substrate degradation and carbohydrate and triterpenoid metabolism during growth was analyzed by combining changes in metabolite content and changes in related enzyme expression in G. lucidum over 5 growth phases. Changes in low-polarity triterpenoid content were correlated with changes in glucose and mannitol contents in fruiting bodies. Additionally, changes in medium-polarity triterpenoid content were correlated with changes in the lignocellulose content of the substrate and with the glucose, trehalose, and mannitol contents of fruiting bodies. Weighted gene coexpression network analysis (WGCNA) indicated that changes in trehalose and polyol contents were related to carbohydrate catabolism and polysaccharide synthesis. Changes in triterpenoid content were related to expression of the carbohydrate catabolic enzymes laccase, cellulase, hemicellulase, and polysaccharide synthase and to the expression of several cytochrome P450 monooxygenases (CYPs). It was concluded that the products of cellulose and hemicellulose degradation participate in polyol, trehalose, and polysaccharide synthesis during initial fruiting body formation. These carbohydrates accumulate in the early phase of fruiting body formation and are utilized when the fruiting bodies mature and a large number of spores are ejected. An increase in carbohydrate metabolism provides additional precursors for the synthesis of triterpenoids. IMPORTANCE Most studies of G. lucidum have focused on its medicinal function and on the mechanism of its activity, whereas the physiological metabolism and synthesis of bioactive substances during the growth of this species have been less studied. Therefore, theoretical guidance for cultivation methods to increase the production of bioactive compounds remains lacking. This study integrated changes in the lignocellulose, carbohydrate, and triterpenoid contents of G. lucidum with enzyme expression from transcriptomics data using WGCNA. The findings helped us better understand the connections between substrate utilization and the synthesis of polysaccharides and triterpenoids during the cultivation cycle of G. lucidum. The results of WGCNA suggest that the synthesis of triterpenoids can be enhanced not only through regulating the expression of enzymes in the triterpenoid pathway, but also through regulating carbohydrate metabolism and substrate degradation. This study provides a potential approach and identifies enzymes that can be targeted to regulate lignocellulose degradation and accelerate the accumulation of bioactive substances by regulating substrate degradation in G. lucidum.

Development and Optimization of the Triterpenoid and Sterol Production Process with Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum Strain G0017 (Agaricomycetes), in Liquid Submerged Fermentation at Large Scale.

Ganoderma lucidum mycelia are rich in active substances such as triterpenoids and sterols. However, reports on the development of effective submerged fermentation processes are lacking and the resulting total triterpene and sterol yield is still quite low. In this study, a new G. lucidum strain G0017 mycelium isolated by screening was studied in a 3-L fermenter to investigate the effect of aeration rate in liquid submerged fermentation production of triterpenoids and sterols. By fitting the specific mycelial growth rate and the specific production rate of the triterpenoid and sterol model, an effective multistage aeration rate control process for triterpenoid and sterol fermentation production was developed. This process was validated and proven in 3-L and 50-L fermenters. The resulting yields of triterpenoids and sterols were 3.34 and 3.46 g/L, respectively, which were 69.54% and 75.63% higher than the fixed aeration rate of 1.50 volume of air per volume of liquid per minute. This optimized fermentation production process conceivably could be applied to larger-scale industrial production and perhaps also to improve liquid submerged fermentation processes with relevant edible and medicinal mushrooms.

BIOTECHNOLOGICAL POTENTIAL OF THE SIBERIAN STRAINS OF BASIDIOMYCETES – PRODUCERS OF LIG-NOLYTIC AND CELLULOLYTIC ENZYMES

The results of a study of the wood-destroying properties of Siberian strains of xylotrophic basidiomycetes (Armillaria, Ganoderma, Fomitopsis, Heterobasidion and Porodaedalea) are presented. The growth parameters and enzymatic activity of the strains were determined during solid-phase and deep cultivation. Fomitopsis pinicola and Ganoderma lucidum are fast-growing fungi on cellulose-, tannin-containing nutrient media, coniferous and deciduous plant substrates (source and hydrodynamically activated). The growth coefficient on media with tannin and Na-carboxymethyl cellulose is more than 45; on plant substrates - more than 30. The strains exhibit a different growth reaction to the preliminary activation of birch sawdust (growth acceleration / growth slowdown / indifference). The maximum wood-destroying activity on wood of A. sibirica noted for the fungi A. borealis, G. tsugae, G. lucidum, F. pinicola, and P. niemelaei. The decrease in substrate mass was 8–11%; the amount of polysaccharides decreased on average 1.4 times mainly due to the fermentolysis of hard-hydrolyzable polysaccharides. High enzymatic activity of fungi observed during solid-phase and deep cultivation with an inducer. The maximum activity of phenol oxidase is characteristic of G. tsugae (1.21 units/g·s); carboxymethyl cellulase – for F.pinicola and G. lucidum strains (11.8 and 10.3 units/ml, respectively); xylanases – for H. abietinum (3.8 u/ml). The maximum accumulation of extracellular protein observed in F. pinicola (0.89 mg/ml). According to the totality of rapid test indicators, quantitative determination of enzymatic activity, growth parameters on lignocellulosic substrates and the degree of wood bioconversion, the most promising producer of lignolytic enzymes in vitro is the Gl4-16A Ganoderma lucidum strain; cellulolytic enzymes – strain Fp6-17 Fomitopsis pinicola.

The whole-genome sequencing and analysis of a Ganoderma lucidum strain provide insights into the genetic basis of its high triterpene content

• A longer and more complete Ganoderma lucidum genome was obtained. • A total of 14,398 genes encoding 15,964 proteins were identified from the Ganoderma lucidum Lingjian-2 genome. • A total of 360 genes in the Ganoderma lucidum Lingjian-2 genome were involved in triterpene biosynthesis. • The most abundant genes related to triterpene biosynthesis are cytochrome P450 genes.

Proximate analysis, phtochemical screening and antioxidant activity of different strains of Ganoderma lucidum (Reishi Mushroom)

In this study, proximate analysis, phytochemical screening and antioxidant activity of two strains of medicinal mushroom <em>Ganoderma lucidum</em> (arbitrarily named strain 5 and 7) and their mix, cultivated in Bangladesh National Mushroom Development Institute, have been determined.

Comparison of Anti-oxidant Activity of Five Elected Wild Commercial Mushrooms in Four Different Solvents as an Alternative Oxidative Stress Reliever

Mushroom also known as a lit source of nutritional and therapeutical component so in order toevaluate the usefulness of the Pakistani wild mushroom strains as the possible nutracueticals, adetailedstudy entailing several tests for the determination of anti-oxidant potential was carried out on the electedmushrooms in four different solvents. Strains of Lentinus edodes, Pleurotus ostreatus, Volvariella volvacea,Pleurotus eryngii and Ganoderma lucidum were extracted for this particular purpose in the water, n- butanol,n-hexane and choloroform solvents. Test models including Total Phenolic Content assay, Total FlavonoidContent assay, 2,2-diphenyl-1,1-picrylhydrazyl (DPPH) Scavenging Activity assay and Reducing Powerassay were utilized for carrying out the comparative analysis among the selected mushrooms. The acquiredresults indicated that the extract of Volvariella volvacea and Pleurotus eryngii possess copacetic anti-oxidantpotential and can be utilized as oxidative stress reliever in humans. Furthermore, extraction studies alsoindicated that water is the most suitable solvent for anti-oxidants extraction for these mushrooms. Finding ofthe anti-oxidant potential for elected wild mushrooms contra indicate that these mushrooms can be safelyexploited as a amended natural anti-oxidant in comparison to its synthetic counterpart.

Influence of PacC on the environmental stress adaptability and cell wall components of Ganoderma lucidum

The transcription factor PacC/Rim101 participates in environmental pH adaptation, development and secondary metabolism in many fungi, but whether PacC/Rim101 contributes to fungal adaptation to environmental stress remains unclear. In our previous study, a homologous gene of PacC/Rim101 was identified, and PacC-silenced strains of the agaricomycete Ganoderma lucidum were constructed. In this study, we further investigated the functions of PacC in G. lucidum and found that PacC-silenced strains were hypersensitive to environmental stresses, such as osmotic stress, oxidative stress and cell wall stress, compared with wild-type (WT) and empty-vector control (CK) strains. In addition, transmission electron microscopy images of the cell wall structure showed that the cell walls of the PacC-silenced strains were thinner (by approximately 25–30%) than those of the WT and CK strains. Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78–80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression. The ability of PacC to bind to the promoters of glucan synthase-encoding genes confirms that PacC transcriptionally regulates these genes.

Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation

Abstract A rare wild-Serbian Ganoderma applanatum strain BGS6Ap (GASB) was morphologically identified based on its basidiocarp with brownish-red cap and woody stipe. Molecularly, GASB DNA was extracted, sequenced (651 bp) and subjected to phylogenetic analysis with evolutionary distance (Knuc). A plasmid Editor (ApE) software was used to verify the sequence and GASB was found to be 99% similarity to the commercial G. applanatum (GA) ATCC44053. The production of GASB's biomass and endopolysaccharide (ENS) in different temperature was screened at 20 °C, 25 °C and 30 °C via one-factor-at-a-time (OFAAT) scheme in a submerged liquid fermentation (SLF). Lower temperature was chosen due to GASB's natural habitat, which belongs to temperate continental climates with the influence of air currents from the Carpathians and the Mediterranean. Using the OFAAT data, the SLF was further optimised using response surface methodology (RSM) to generate biomass-ENS based on temperature (20°C–30 °C), agitation rate (100–200 rpm), glucose concentration (10 g/L–50 g/L) and initial pH (4–6). The highest production of biomass (17.51 g/L) and ENS (3.47 g/L) was generated using optimal conditions of 25 °C, 150 rpm, 10 g/L of glucose and pH 6. To study the structural composition, ENS confirmed spectral linkages in FTIR (2925 cm−1, 1623 cm−1, 1073 cm−1, 890 cm−1) and NMR ( δ 4.30, 4.80, 5.20 ppm) as endo-β-D-glucan. This approach could serve as a blueprint for novel mycelial biomass and endo-β-D-glucan production of GASB which can be further studied on different temperate basidiomycetes.

Morphological Characteristics, Yield Performance, and Medicinal Value of Some Lingzhi Mushroom (Ganoderma lucidum) Strains Cultivated in Tam Dao, Vietnam

The aim of this study was to evaluate the biological efficiency and main bioactive components of three G. lucidum strains, viz. GA1, GA2, and GA3, cultivated in Tam Dao town. The results demonstrated that all strains were capable of growing well on PDA medium supplemented with rice bran. The time required for complete colonization was 9 days. All tested strains of G. lucidum were able to adapt to climate conditions and produce fruiting bodies with satisfactory yield (13-17%), and therefore, they could be considered suitable candidates for commercial cultivation of G. lucidum in Tam Dao. No significant differences in polysaccharide content were observed among all strains. High concentrations of lucidenic N acid (0.33 mg g-1) and ganoderic acid (2.38 mg g-1) were determined in strain GA3. However, the highest ganodermanontriol content was detected in the strain GA1 with 0.3 mg g-1.

Evaluación del cultivo de Pleurotus ostreatus y Ganoderma lucidum (Agaricomicetes, Agaricales – Poyporales) empleando sustratos alternativos

Con el propósito de cultivar cepas de Pleurotus ostreatus y Ganoderma lucidum se realizaron ensayos con cinco sustratos experimentales realizando mezclas en pro- porciones distintas. Como inóculo se utilizaron semillas de porotos y garbanzos, granos de avena y cebada, utilizando los granos de avena como control positivo de crecimiento del micelio. Se prepararon los sustratos de cultivo en bolsas de poli- propileno con 1/2Kg y 1 kg de sustrato húmedo de virutas de madera, bagazo de caña procesado, hojarascas y granos de avena en baja proporción para enriquecer el sustrato, utilizando las virutas de Cedrela fissilis como control positivo; las bolsas se esterilizaron por dos h a 120°c, 1 atm de presión y luego se inocularon y fueron incubadas en la oscuridad a 18/25°c de temperatura y aproximadamente 70% de hu- medad ambiente, durante 22-35 días. Una vez cubierto de micelio el sustrato, se pasó a la inducción con 12 h de luz y oscuridad, riego de dos veces por día, temperatura y humedad constantes. Al cabo de 30 a 35 días ya se obtuvieron basidiomas. Se evaluó la eficiencia biológica (EB%) desde el momento de la inducción hasta la cosecha. El sustrato con mejores resultados después del control (53,20%EB) fue la mezcla de virutas con avena y hojarascas con una EB (45,20%). Se obtuvieron fructificaciones en todos los sustratos con excepción de bagazo de caña. La utilización de desechos orgánicos para el cultivo de hongos puede resultar una interesante alternativa de reciclaje de materia orgánica para generar cultivos a pequeña o gran escala.

Optimal culture conditions for mycelial growth and fruiting body formation of Ling Zhimushroom Ganoderma lucidum strain GA3

The objective in this study is to investigate optimal culture conditions for mycelial growth and fruiting body formation of the Ling Zhi mushroom, Ganoderma lucidum strain GA3. The results of the study show that the optimal media and temperature for the mycelial growth are potato, glucose, and agar (PGA) supplemented with rice bran, and 25-300C, respectively. Strain GA3 is able to grow in a wide pH range, between 4 and 12.The most favourable substrate mixture for the formation and development of the fruiting body is 87% sawdust + 4% corn powder + 8% rice bran + 1% calcium carbonate (CaCO3).

SNP and SCAR Markers for Specific Discrimination of Antler-Shaped Ganoderma lucidum.

In this study we identified single nucleotide polymorphism (SNP) and sequence characteristic amplification region (SCAR) markers for specific identification of antler-shaped Ganoderma lucidum strains. When the partial mitochondrial SSU rDNA gene sequence of various antler- and kidney-shaped G. lucidum strains were analyzed and aligned, an SNP was found only in the antler-shaped G. lucidum strain at position 456 bp. In addition, this SNP of antler-shaped strains was digested by HinfI restriction enzyme. We further analyzed the polymorphism of various G. lucidum strains by random amplified polymorphic DNA (RAPD) analysis. In RAPD analysis, we isolated and sequenced a fragment, specific for antler-shaped G. lucidum strains. Based on this specific fragment sequence, two sets of specific primer pairs for antler-shaped G. lucidum strains were designed. PCR analysis revealed that two specific bands were observed only from antler-shaped strains. These two molecular markers will be helpful for identification of morphological characteristics of G. lucidum.