Optimization of Growth Conditions and Biological Activities of Nepalese Ganoderma lucidum Strain Philippine.

Krishna Subedi,Manisha Neupane,Raju Panday,Giri Raj Tripathi,Buddha Bahadur Basnet

doi:10.1155/2021/4888979

Abstract

Ganoderma lucidum has been extensively studied for its valuable medicinal importance. In this study, the artificial cultivation of G. lucidum strain Philippine in different culture media, including sawdust substrate, was performed and optimized on the Potato Dextrose Agar (PDA) media. Phytochemical, antibacterial, and antioxidant analyses were performed and compared between the ethanol extracts prepared from two different cultures (fruit from synthetic log culture and mycelia from PDA media culture). Both the 200 mg/mL and 100 mg/mL concentrations of extracts inhibited all the tested bacteria, and the results were promising than the corresponding control using antibiotics. The fruit extract showed higher antioxidant potential (150.6 ± 56.92 mg ascorbic acid equivalent/g extract) than mycelial extract (144.28 ± 81.72 mg ascorbic acid equivalent/g extract). The results indicate that fruiting bodies of G. lucidum cultivated in a complex dust medium possess higher antioxidant properties than mycelia culture, which can be further explored for therapeutic applications.

Highlights

Ganoderma (Basidiomycota, Polyporales) is one of the wellknown medicinal polypore fungi in the family Ganodermataceae and is widely used in East Asia, America, and other countries [1]
G. lucidum strain Philippine was purchased from the Centre for Agricultural and Technological Training, Lalitpur, and cultured in Potato Dextrose Agar (PDA) (Hi-Media, Mumbai, India) plates and slants. e cultured plates were kept at 4°C for research purposes
Saw dust-powered supplement with raw wheat bran and raw rice bran was used as the previous investigation on the cultivation of G. lucidum using different biomasses which showed saw dust is a good substrate among different substrates [17]

Summary

Introduction

Ganoderma (Basidiomycota, Polyporales) is one of the wellknown medicinal polypore fungi in the family Ganodermataceae and is widely used in East Asia, America, and other countries [1]. More than 400 species, only red, white, black, yellow, blue, and purple Reishi have been explored for their potential health-beneficial properties [2]. Of them, red Reishi (G. lucidum) and black Reishi (G. sinensis) have shown the most significant health-strengthening effects [3]. East Asian countries consumed Reishi for their medicinal value rather than nutritional value to extend the life span and increase youthful vigour and vitality [1]. G. lucidum, a cosmopolitan mushroom species, is a polypore rack mushroom that changes color during the morphogenesis process from orange-white to bright red. It has been reported that G. lucidum contains over 400 bioactive compounds, which have several medicinal effects, remarkably such as antitumor and anti-inflammatory, antimicrobial, antifungal, antiviral ( against herpes and HIV) effects, antioxidative, and radical scavenging effects [5,6,7,8,9]

Methods

Results

Conclusion