Abstract
Mushroom also known as a lit source of nutritional and therapeutical component so in order toevaluate the usefulness of the Pakistani wild mushroom strains as the possible nutracueticals, adetailedstudy entailing several tests for the determination of anti-oxidant potential was carried out on the electedmushrooms in four different solvents. Strains of Lentinus edodes, Pleurotus ostreatus, Volvariella volvacea,Pleurotus eryngii and Ganoderma lucidum were extracted for this particular purpose in the water, n- butanol,n-hexane and choloroform solvents. Test models including Total Phenolic Content assay, Total FlavonoidContent assay, 2,2-diphenyl-1,1-picrylhydrazyl (DPPH) Scavenging Activity assay and Reducing Powerassay were utilized for carrying out the comparative analysis among the selected mushrooms. The acquiredresults indicated that the extract of Volvariella volvacea and Pleurotus eryngii possess copacetic anti-oxidantpotential and can be utilized as oxidative stress reliever in humans. Furthermore, extraction studies alsoindicated that water is the most suitable solvent for anti-oxidants extraction for these mushrooms. Finding ofthe anti-oxidant potential for elected wild mushrooms contra indicate that these mushrooms can be safelyexploited as a amended natural anti-oxidant in comparison to its synthetic counterpart.
Highlights
Generation of the reactive oxidative groups as by products owing to the metabolic activities of human beings could be regarded as the phenomenon of natural occurrence
Fresh, refined and shade-dried strains of Lentinusedodes, Pleurotusostreatus, Volvariellavolvacea, Pleurotuseryngii and Ganodermalucidumwere personally acquired from Medicinal Mushroom Lab Institute of Horticultural Sciences, University of Agriculture, Faisalabad
Following assays were used for the evaluation of anti-oxidant activity of the selected wild mushrooms. 2.3.1
Summary
Generation of the reactive oxidative groups as by products owing to the metabolic activities (normal/pathological) of human beings could be regarded as the phenomenon of natural occurrence. In Asian region, the fruiting bodies/caps of wild mushrooms is favored as the traditional cuisine component (owing to its explicit taste and rich aromatic qualities)and is considered one of the highly regarded therapeutic and nutritional supplement employed excessively in human diet for dealing with illnesses of hypertension, cancer and hypercholesterolemia [4]. This is attributed to the amassing of various secondary metabolites like steroids, poly-ketides, terpenes and phenolic compounds in the tissue of the mushrooms [5].
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