A series of some novel sulphur and nitrogen containing ferrocenyl linked heterocyclic compounds were synthesized by multistep reactions and evaluated for in vitro antimicrobial activity against 15 ATCC strains out of which 8 were bacterial (Pseudomonas aeruginosa, Streptococcus bovis, Enterococcus faecalis, Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae, Methicillin-resistant Staphylococcus aureus and Streptococcus mutans) and 7 were fungal (Candida albicans, Candida dubliniensis, Candida glabrata, Candida parapsilosis, Candida tropicalis, Candida kefyr and Candida krusei) strains. The results clearly depict that the compounds (1–12) gave an average antimicrobial activity against the tested strains with an exception of compound 12 which stood out in terms of its activity against the tested organisms. All these compounds gave a range of MIC value between 32–64μg/ml against S. bovis, E. coli and C. tropicalis except compound 12 which gave a MIC of 16μg/ml against each of them. The MIC values of all these compounds against biofilm forming P. aeruginosa and S. mutans were 64–256μg/ml and 64–128μg/ml respectively which is apparently high, concluding that these compounds hold immense potential to be employed as a two in one formulation of antibacterial as well as antifungal agents.