ABSTRACT Innovative biological processes that utilize the power of microorganisms are interesting tools for the treatment of Heavy metals polluted soils. This study conducted in column systems containing three different soils artificially contaminated with Cr, Zn and Cu, investigates the effect of soil bioaugmentation by two yeast strains Wickerhamomyces anomalus and Candida tropicalis on heavy metal leaching and the behavior of soil enzymatic activities. The soils have different capacity to retain heavy metals and the correlation tests showed the influence of soil properties, mainly CaCO3, clay, silt and aromaticity of organic matter on the mobilization of these heavy metals. Strong positive correlation was also observed between enzymes activities (phosphatase, galactosidase and arylsulfatase) and organic matter. The introduction of yeast strains was beneficial. It favored the immobilization of Cr and the mobilization of Cu and Zn in soils. It also stimulated the soil phosphatase, galactosidase and arylamidase activities and affected negatively arylsulfatase activity. Data thus indicate that the bioaugmetation of soils with yeast cells present an excellent performance in bioremediation by decreasing/increasing the bioavailability of metals and on soil ecosystem functioning by increasing soil enzyme activity.