Aktywność in vitro olejku świerkowego (Oleum Picea excelsa) wobec grzybów drożdżopodobnych z rodzaju Candida

Abstract

Introduction. Spruce (Picea abies L.) is a tree belonging to Pinaceae family (Pinaceous). It is large tree (to 50 m heigh), which produce neesdes and cones. Spruce essential oil, is used in medicine. Its components are following: borneol, limonene, camphora, bornyl acetate, α- and β-pinene, 3-carene, β-phellandrene, camphene and cadinene. The oil exhibited antimicrobial activity towards bacterial, viruses, yeastlike fungi and mycoses. Aim. The aim of this study was to evaluate the activity of spruce oil on yeastlike fungi. Material and methods. The data included 31 yeastlike fungi isolated from patients from oral cavity. The strains belonging to the genus: Candida albicans (15 strains), C. glabrata (2), C. guilliermondii (2), C. humicola (1), C. kefyr (2), C. krusei (2), C. lusitaniae (1), C. parapsilosis (3), C. tropicalis (2) and C. utilis (1). Moreover investigated 9 reference strains. The susceptibility (MIC) yeastlike fungi to spruce oil (Semifarm) was determined by plate dilutions technique in Sabouraud agar. The inoculums contained 105 CFU per spot. The agar plate were inoculated using the Steers replicator. Incubation of agar plates was performed in aerobic conditions, at temp. 37°C for 24-48 hours. The MIC was defined as the lowest concentrations of spruce oil completely inhibited growth of tested strains of yeastlike fungi. Results. The results indicated, that tested strains of yeastlike fungi were susceptible to oil in concentrations 7.5-≥ 20.0 mg/ml. The growth of the strains from genus Candida albicans was inhibited by concentrations 7.5-≥ 20.0 mg/ml. The spruce oil was active on Candida glabrata strains in concentrations 10.0-20.0 mg/ml. The less sensitivity characterized the genus of Candida lusitaniae, Candida utilis (MIC = 15.0 mg/ml), Candida krusei and Candida parapsilosis (MIC 15.0-≥ 20.0 mg/ml). The oil was the lowest active towards strains of Candida guilliermondii, Candida humicola, Candida kefyr and Candida tropicalis. The growth of these strains was inhibited by concentrations 20.0 mg/ml and high. Conclusions. The strains from genus of Candida were the moderate susceptible to spruce oil. The oil was the lowest active towards strains from genus Candida guilliermondii, Candida humicola, Candida kefyr i Candida tropicalis.