Oddziaływanie olejku cyprysowego (Cupressi aetheroleum) na bakterie mikroaerofilne

Abstract

Introduction. The evergreen cypress (Cupressus sempervirens L.) belongs to the cypress family (Cupressaceae). It is considered a medicinal plant, and extracts from leaves, fruits and seeds are used in many ailments. The essential oil obtained from young twigs and cones of evergreen cypress contains, among others: α-pinene, δ3-carene, limonene, α-terpinene, β-myrcene, cedrol, β-pinene, δ-caryophyllene and β-caryophyllene, has antimicrobial activity and is recommended for external use. Aim. Testing the activity of cypress oil against microaerophilic bacteria occurring in the oral cavity. Material and methods. The microaerophilic bacteria for the study were isolated from the oral cavity They belonged to the species: Aggregatibacter actinomycetemcomitans, Campylobacter sputorum, Eikenella corrodens and Rothia dentocariosa. The date obtained 5 reference strains to. The susceptibility (MIC) was determined by means plate dilution technique in Brucella agar. The concentrations of cypress oil (Avicenna-oil, Wrocław) were: 5.0, 7.5, 10.0, 15.0 and 20.0 mg/ml. The inoculums containing 105 microorganisms in 1 ml were seeded with Steers replicator. Incubation performed in microaerophilic conditions in anaerobic jar with CAMPY Pak, BBL and standards strains in anaerobic atmosphere in anaerobic jars, in 37°C for 48 hours. The MIC was interpreted as the lowest concentration of cypress oil that completely inhibited the growth of tested microaerophilic bacteria. Results. The results indicated that the cypress oil was the most active in concentrations ≤ 5.0-7.5 mg/ml against 42% tested bacteria. Another concentrations in ranges 10.0-15.0 mg/ml was inhibited growth 26% strains. But 32% strains were susceptible on concentration 20.0 mg/ml and more. Tested cypress oil was the most active against strains from genus Rothia dentocariosa (MIC < 5.0-7.5 mg/ml). The genus Aggregatibacter actinomycetemcomitans was the lowest sensitive. The growth of this strains was inhibited in concentrations in ranges 5.0-≥ 2.0 mg/ml. Cypress oil was the most active at concentrations < 5.0-7.5 mg/ml against 47% of the assessed microaerophilic bacteria. Concentrations in the range of 10.0-15.0 mg/ml inhibited the growth of 26% of microorganisms. In contrast, 32% of the strains were sensitive to concentrations of 20.0 mg/ml and more. The tested cypress oil showed the highest activity against strains of the Rothia dentocariosa species (MIC < 5.0-7.5 mg/ml). Aggregatibacter actinomycetemcomitans was characterized by the lowest sensitivity. The growth of these bacteria was inhibited by concentrations of 5.0-≥ 20.0 mg/ml. Conclusions. Cypress oil was characterized by moderate activity against the tested bacteria growing in microaerophilic conditions, and the strains of the Rothia dentocariosa species showed the highest sensitivity to this oil.