Stimulation Of Reparative Processes Research Articles

Результаты воздействия электромагнитных волн терагерцового диапазона на ткани локтевого сустава при последствиях его повреждений

Introduction Among the ranges of electromagnetic waves used in clinical practice, electromagnetic waves of the terahertz range (EMWTHR) have promising applications. The experimental and clinical studies show that they are able to provide correction of the main pathophysiological disorders of a living organism such as hypoxia, hypercoagulation, and immunodeficiency. Purpose To study the effectiveness and safety of terahertz electromagnetic waves in the complex treatment of patients with the consequences of fractures of the humeral condyle Methods Clinical, radiological, physiological and laboratory studies were conducted in 30 patients with consequences of fractures of the humeral condyle (post-traumatic deformities, osteoarthritis of the elbow joint). Surgical treatment of patients with consequences of injuries of the elbow joint was classical and consisted of corrective osteotomies and osteosynthesis with the Ilizarov apparatus of the humerus and forearm. The main group consisted of 15 patients who underwent 10 sessions of EMWTHR exposure to the osteotomy zone, and 15 patients did not undergo physiotherapy. Results It was found that in patients of the main group, the average values of the intensity of pain and the deficit in the range of motion were significantly lower than the findings of the comparison group in the short term after treatment. The use of EMWTHR therapy led to differences in metabolic processes in the compared groups. There were no significant changes in radiological, physiological and laboratory parameters, as well as clinical signs that could be attributed to adverse events or complications associated with the use of EMWTHR. Conclusion The results of the comparative study allow us to recommend the EMWTHR therapy in the system of complex treatment of patients with the consequences of fractures of the humeral condyle and can be used as a means for local stimulation of reparative processes in target patients.

Problems of lengthening of the lower limbs in children and stimulation of reparative processes in the area of distraction regenerates

During the period from 1988 to 2000 the compensation lower limb shortening was performed in 285 patients, aged 2.5-18 years, at CITO Pediatric Orthopaedic Clinik. In 5 patients with severe extremity malformation and severe vascular pathology surgical interventions aimed at facilitation of subsequent prosthesis and orthesis. The other patients were subjected to surgical correction using rod distraction device of original design. Complex rehabilitation treatment including functional biocontrol was started in the early postoperative period. The condition of distraction regenerate was evaluated by X-ray, CT and Doppler Laser Flowmetry data. In 63 patients with congenital lower extremity pathology in whom insufficient regeneration was observed the implants containing fetal bone tissue were inserted into the regenerate zone resulting in marked osteogenic effect. Suggested treatment allowed to improve the outcomes and shorten the terms of treatment.

Аналіз застосування Мексикору у хворих із гострим коронарним синдромом

Матеріали та методи. Під спостереженням знаходилися дві групи хворих із гострим коронарним синдромом. Перша група включала 48 хворих із нестабільною стенокардією (НС) віком 58,5 ± 3,8 року. Діагноз НС верифікований на підставі скарг хворих і змін на електрокардіограмі — підйому або депресії інтервалу на 1 мм тривалістю не більше 3 діб, відсутності зон гіпокінезії при УЗД серця, негативного тропонінового тесту. Другу групу становили 45 хворих із гострим інфарктом міокарда. Діагноз верифіковано за допомогою тропонінового тесту і змін на ЕКГ. У цю групу включалися хворі з Q-позитивним гострим інфарктом міокарда (ГІМ). Чоловіки — 83,2 %, жінки — 16,8 %. Середній вік хворих становив 60,3 ± 1,2 року. Задньобазальна локалізація виявлена у 40,2 %, передня — у 59,8 %. В анамнезі у 70 % хворих гіпертонічна хвороба ІІ ст. «Стаж» ішемічної хвороби серця становив у середньому 10,8 ± 4,5 року. 80 % хворих цієї групи неодноразово лікувалися в кардіологічних стаціонарах із приводу загострень ішемічної хвороби серця. У 38,5 % хворих на момент надходження відзначені надшлуночкові і шлуночкові (екстрасистолія) порушення ритму. Результати. Застосування Мексикору у хворих із ГІМ значно покращувало систолічну функцію серця, що проявлялося у вірогідній різниці основних показників, що її відображають (фракція викиду, швидкість циркуляторного скорочення волокон, серцевого викиду), і відповідних показників у хворих контрольної групи. Подібні сприятливі зміни були виявлені і при вивченні в динаміці показників діастолічної функції серця, що визначаються за швидкістю трансмітрального потоку. Обговорення. Зазначені зміни ми пов’язуємо з відновленням або поліпшенням скоротливості ішемізованого міокарда та функціонального стану так званих інтактних відділів серця, що компенсують своєю діяльністю втрату скоротливості некротизованих ділянок. Це підтверджується динамікою стану зон акінезії і дискінезії у хворих на ІМ, де відстежується відновлення скоротливості окремих ділянок серця на фоні застосування Мексикору (перехід граничних із зонами інфаркту сегментів зі стану акінезії в гіпокінез, гіпокинезу — в нормокінез), і практично зворотною динамікою у хворих контрольної групи. Мексикор сприяв поліпшенню клінічного перебігу ІМ, що проявлялося в обмеженні величини вогнища некрозу і стимуляції репаративних процесів, поліпшенні скорочувальної функції серця і газообміну, зменшенні частоти пролонгованого і рецидивного перебігу, ймовірності розвитку аневризми серця і випадків серцевої недостатності, порушень ритму і провідності, недостатності кровообігу і в кінцевому підсумку летальності. За результатами холтерівського моніторування у хворих на НС Мексикор прискорював стабілізацію стенокардії і зменшував, порівняно з контрольною групою, добову частоту і тривалість періодів ішемії міокарда, епізодів порушень ритму, сприятливо впливав на зміну інтеграла зміщення ST і співвідношення больових/безбольових періодів ішемії. Висновки. Включення Мексикору в традиційну терапію гострого коронарного синдрому вже на догоспітальному етапі дозволяє істотно зменшити прояви окислювального стресу, сприяє нормалізації діяльності серцево-судинної системи і покращує клінічний перебіг захворювань.

Сучасні хірургічні методи лікування постраждалих з вогнепальними пораненнями кінцівок

Подано можливості етапного лікування вогнепальних ран у 254 пацієнтів з використанням терапії негативним тиском, ультразвукової кавітації і мікробіологічного дослідження ранового вмісту. Розглянуто підходи в комплексному лікуванні на підставі бактеріологічних властивостей вогнепальних поранень. Аналіз результатів лікування показав, що метод комплексного лікування вогнепальних ран м’яких тканин кінцівок з використанням апарату вакуумної аспірації, ультразвукової кавітації для очищення ран, збагаченої тромбоцитами плазми, для локальної стимуляції репаративних процесів дає можливість скоротити тривалість лікування до 8,0 ± 2,8 дня, знизити кількість ускладнень на 30,0 ± 1,2 %, що дозволяє рекомендувати цей метод лікування даної патології.

Study of the Effectiveness of Drug No. 1 on the Model of Alkaline Eye Burn in Rabbits.

Studies have shown that 0.05 mL/kg of drug No. 1 as 1:15 dilution with sterile saline solution has anti-inflammatory, wound-healing, and angioprotective effects if instilled in both eyes of rabbits twice a day for 30 days after an alkaline burn. A stimulation of reparative processes in the cornea was observed with the test dose of drug No. 1. This was manifested by accelerating the recovery of defects in the anterior epithelium and stroma, reducing the frequency of formation of deep defects and the severity of inflammatory reaction and vascularization, and inhibiting the formation of turbidity of its lower intensity and area. A tendency to restore laminarity of the stroma was determined by the action of drug No. 1 throughout the observation period. This contributed to a decrease in the degree of vascularization and prevented ulceration and perforation of the cornea. By the end of experiment, a restoration of strong epithelial-stromal relationships in the experimental group, compared to the control group, was observed due to formation of normal architectonics of fibrous components of intercellular substance. A more pronounced proliferative activity, with an increase in the layering of limbal epithelial cells, was noted in the limbal zone of the cornea in the experimental group rabbits compared to the control group.

Evaluation of the effectiveness of negative pressure wound therapy in the complex treatment of patients with infected mesh endoprostheses of the anterior abdominal wall

Introduction. Wound complications after the placing of various implants of the abdominal wall after hernia repair account for up to 15 % of all hospitalizations with purulent diseases. Their treatment is often accompanied by a high frequency of explantation of a mesh endoprosthesis with a risk of recurrent hernias in this category of patients.The objective of the study was to improve the results of surgical treatment of patients with infected implants of the abdominal wall after herniaplasty based on the assessment of the effectiveness of the negative pressure wound therapy (NPWT).Methods and materials. A retrospective analysis of the treatment results of patients with infected mesh endoprostheses of the anterior abdominal wall (n=68) was carried out. We formed for comparison two homogeneous representative groups of patients, who underwent adequate debridement of a purulent wound with empirical antibiotic therapy. Patients from the study group (n=38) received local therapy using NPWT, patients from the control group (n=30) received traditional local therapy. The evaluation of the treatment results was carried out according to a number of criteria: the course of the systemic inflammatory reaction, the dynamics of reparative processes in the wound of the anterior abdominal wall, evaluation of the clinical outcomes of treatment of patients.Results. The use of vacuum therapy was accompanied by a positive effect on the course of the systemic inflammatory reaction, stimulation of reparative processes in the wound due to the rapid elimination of bacterial agents from the wound cavity (p= 0.003), as well as significant retraction of the wound cavity (p=0.004) compared to traditional methods of wound treatment. Patients from the study group had more favorable treatment outcomes such as a reduction in the duration of hospitalization (p=0.005) and the number of surgical interventions (p=0.003). Due to the NPWT, it was possible to save implants in 30 patients out of 38 (78.9%) with infected mesh endoprostheses of the anterior abdominal wall, which prevented the formation of recurrent hernias and helped to avoid reoperations in this category of patients.Conclusion. The use NPWT is a safe and effective method for treating wounds of the anterior abdominal wall with infected mesh endoprostheses after hernioplasty.

EXPERIMENTAL RESEARCH OF THE POSSIBILITIES OF INFLUENCE ON HEALING SMALL INTESTINAL ANASTOMOSIS

Abstract. A promising area of further research is the use of a combination of components of tissue growth factors in combination with low-intensity electromagnetic effects of light radiation.
 Objective. Investigation of various methods of influence on the healing of sutures of the small intestinal anastomosis in conditions of peritonitis.
 Materials and methods. The experiment was conducted on 20 white Wistar male rats weighing 180-250 g, the content, care and methods of experimental work with which corresponded to the observance of the International Principles of the European Convention on the Protection of Vertebrate Animals (Strasbourg, 1985)
 Results. The research work reflects the results of an experimental study, the purpose of which was to develop a method of sealing the small intestinal anastomosis under peritonitis. The experiment was performed on 20 white Wistar male rats weighing about 250 grams. The possibilities of using physical (light of a certain wavelength) and biological (tissue growth factors) methods in the prevention and treatment of acute inflammatory reactions and stimulation of reparative processes are shown. It is noted that a promising direction for further research on methods for the prevention of insolvency of small bowel anastomoses is the use of electromagnetic effects of low intensity of the light spectrum in combination with the application of tissue growth factors.
 Conclusion. A promising direction for further research is the use of a combination of sealing lines with the application of components of tissue growth factors in combination with a low-intensity electromagnetic effect of light radiation.

Effect of Morphogenetic Protein BMP-2 on X-Ray Density of Bone Defect in the Experiment.

In experiments on Wistar rats, a simulated defect in the flat bones of the skull was filled with a collagen sponge of animal origin impregnated with BMP-2 or pure sponge; in control rats, the defect was left open. During follow-up, X-ray density of the collagen sponge in the experimental groups differed significantly. The results attest to the absence of spontaneous remodeling of the bone tissue under conditions modeled focal defect. Moreover, stimulation of reparative processes by the collagen matrix did not lead to positive dynamics. Saturation of the collagen sponge with BMP-2 in a concentration of 0.05 mg/ml allowed increasing Xray density of the bone starting from week 4.

COMBINED SURGICAL TREATMENT LIVER ABCESSES IN EXPERIMENT

The results of surgical antibacterial rehabilitation and drainage of liver abscess largely depend on the effectiveness of local action of drugs. A promising direction in experimental liver abscess surgery is the local application of laser and nanotechnology. - The goal is to experimentally substantiate the effectiveness of the combined use of low-intensity laser radiation and copper nanoparticles in the surgical treatment of liver abscesses. Material and methods. In an experiment on 80 laboratory rabbits, a classic liver abscess was modeled. According to the variant of local two-week treatment, the animals were divided into 4 groups of 20 each. Animals of the first group were given daily standard sanitation of the cavity with an antiseptic solution; in the second group, low-intensity laser irradiation was locally administered; animals of the third group, the cavity of the abscess was washed with a suspension of copper nanoparticles; in the fourth group of animals, treatment with a suspension of copper nanoparticles in combination with low-intensity laser irradiation was applied. Clinical, planimetric, microbiological, morphological and instrumental research methods were used to evaluate the results of treatment. Results and discussion. The best clinical indicators of general condition by the 9th day were observed in the treatment of animals of the fourth group. Due to the active stimulation of reparative processes, by the 7th day of treatment of animals of this group, no aspirate cultures from the growth abscess cavity were given, which made it possible to speed up the closure of the purulent cavity, which by the 21st day according to ultrasound planimetry, the abscess cavity of the liver was completely obliterated. Conclusion. Used clinical and instrumental methods of research are effective ways to assess the results of treatment of simulated purulent cavity of the liver. The combined surgical treatment of simulated liver abscesses with topical application of uniformly scattered LLLT and copper nanoparticles provides accelerated cleansing of the abscess cavity causative agent and complete obliteration of the abscess cavity.

Дослідження впливу тканинних факторів росту та електромагнітного випромінювання світлового діапазону на тонкокишкові анастомози в експерименті

Мета. Розробка методу герметизації швів тонкокишкового анастомоза в умовах перитоніту.
 Матеріали і методи. Експеримент виконано на 20 білих щурах-самцях лінії Вістар масою тіла близько 250 г. Догляд за тваринами, зміст та методи експериментальної роботи відповідали принципам Європейської конвенції щодо захисту хребетних тварин, що використовуються для дослідних та інших наукових цілей (Страсбург, 1986).
 Результати. Проведеним експериментальним дослідженням показано можливості застосування фізичних (хвилі світла певної довжини) та біологічних (тканинні фактори росту) методів у профілактиці і лікуванні гострих запальних реакцій та стимуляції репаративних процесів.
 Висновки. Перспективним напрямом подальшого дослідження методів профілактики неспроможності тонкокишкових анастомозів є застосування електромагнітного впливу низької інтенсивності світлового спектра в комбінації з аплікацією тканинних факторів росту.

Experimental justification of the use of electromagnetic radiation of the light range and tissue growth factors in the treatment of the failure of intestinal anastomosis

The research work reflects the results of an experimental study, the purpose of which was to develop a method of sealing the small intestinal anastomosis under peritonitis. The experiment was performed on 20 white Wistar male rats weighing about 250 grams. The possibilities of using physical (light of a certain wavelength) and biological (tissue growth factors) methods in the prevention and treatment of acute inflammatory reactions and stimulation of reparative processes are shown. It is noted that a promising direction for further research on methods for the prevention of insolvency of small bowel anastomoses is the use of low-intensity electromagnetic radiation in visible spectrum in combination with the application of tissue growth factors.

Histochemical picture in the skin of rats 1, 3, 7, 14, 21 and 30 days after burning of ІІ-ІІІ degrees on the background of injection during first 7 days of 0.9% NaCl solution

For pathogenetically grounded therapy of burn disease, aimed at reducing the severity of the pathological process, the rapid recovery of microcirculation and stimulation of reparative processes, it is necessary to study the fine mechanisms of reparative regeneration in the skin on the microscopic and biochemical levels. The purpose of the study is to investigate the state of elastic and collagen fibers, as well as glycoproteins and glycosaminoglycans in the intercellular substance of the dermis of rats 1, 3, 7, 14, 21 and 30 days after the burning of the II-III degree, with the background of the injection during first 7 days of 0.9% NaCl solution. An experimental study of structural changes in the skin after burn injury was performed on laboratory white rats, males weighing 150-160 g. Rats were divided into 2 groups, which previously, under the conditions of propofol anesthesia 60 mg/kg internally, catheterization of the femoral vein and depilation lateral surfaces of the trunk of rats. Group 1 - animals without thermal trauma of the skin, which once a day for the first 7 days were injected intravenous infusion of 0.9% NaCl solution in a dose of 10 ml per kg. Group 2 - animals who were also injected once a day with the first 7 days of infusion of 0.9% NaCl solution in a dose of 10 ml per kg after 2-3 degree skin burns, with a total area of 21-23% of the body surface of the rat. Euthanasia of rats was performed after propofol anesthesia (60 mg/kg i/v) by decapitation. Changes in the histochemical structure of bits of skin from the edge of the wound were studied after 1, 3, 7, 14, 21, and 30 days from the beginning of the experiment. Histological sections 5-6 microns thick were stained with Weigert’s elastic stain and dyed with picric acid by Van Gieson’s method (for the detection of elastic and collagen fibers); the PAS + Hale reaction was performed by the Moury method (detection of glycoproteins and glycosaminoglycans in the intercellular substance of the dermis). The histochemical investigations of the area of the II-III degree of skin damaged by burns on the background of the injection of 0.9% NaCl solution found that the degree of change depends on the duration of the experiment. In the early stages of the experiment, the reorganization of the intercellular substance of the dermis manifests itself as damage to the fibrous structures of its papillary and reticular layers. Histochemically, the components of amorphous connective tissue substance are dominated by glycoproteins, the content of acidic glycosaminoglycans is small. In the late stages of the experiment (after 14, 21 and 30 days) in the connective tissue of the affected skin in the central and marginal regions poorly structured collagen and elastic fibers are observed. The pronounced “Hale”-positive coloration reflects an increase in the content of sulfated glycosaminoglycans in the amorphous substance of the connective tissue. Thus, the experimental thermal trauma of skin II-III degree on the background of the introduction during the first 7 days, 0.9% of the NaCl solution is histochemically characterized by reorganization of the intercellular substance of the connective tissue of the dermis. In the early stages of the experiment there is damage to the fibrous structures of the papillary and reticular layers, the prevalence of glycoproteins and the insignificant content of acidic glycosaminoglycans in the amorphous substance, indicating a significant inhibition of adaptive-compensatory processes. In the long term, after the thermal defeat in the dermis, histochemically, an increase in the amount of acid glycosaminoglycans is detected and the PAS positivity of the collagen fibers of the intercellular substance is well expressed. This indicates a slow updating of the amorphous substance and fibrous structures of the connective tissue of the dermis, a violation of regenerative processes.

Functional characteristics of peripheral blood mononuclear cells after administration of granulocyte colony-stimulating factor in patients with chronic heart failure

It is shown that the introduction of granulocyte colony stimulating factor (G-CSF) in patients with chronic heart failure increases the functional activity of peripheral blood mononuclear cells (MNC). Enriching peripheral blood MNCs with progenitor cells (PC) by mobilization of bone marrow leads to an increase of their proliferative capacity both under spontaneous culture conditions and when stimulated with ConA mitogen and cytokines G-CSF. Proliferative activity of MNCs is found to increase, as the number of CD34+ PC starts growing. It is also noted that MNCs produce a wide range of cytokines with regulatory action after mobilization in a 48-hour culture in vitro. The findings suggest the feasibility of using MNCs after G-CSF mobilization for stimulation of reparative processes in the myocardium.

The Macrophages as the Stimulators of B‐cells Proliferation in the Pancreas

The main reason for the development of type 1 diabetes is decrease of the absolute number of B‐cells and their functional activity. The goal of our study was to investigate the regulation of B‐cells regeneration and function.The model of alloxan diabetes was simulated on male Wistar rats according the author's modified method. The treatment of animals responded EC Directive 2010/63/EU. The content of macrophages was determined by the immunohistochemical study of the pancreatic sections (MAC387) (Abcam), and the number of proliferating B cells was detected with double immunohistochemical staining (insulin antibodies (clone E11D7, Millipore) and Ki67 (BD)). The number and size of pancreatic islets were counted. The derivatives of aminoftalhydrozide were used as the modulators of macrophage activity (patent). The density of B‐cells, their proliferative and secretory activity were significantly decreased of the pancreas of diabetic animals, the average size of islets was also decreased. The number of macrophages in the pancreas islets of diabetic animals was drastically increased. This was reduced by half after immunomodulation. The result of immunomodulation was the increase of islets' size, B‐cells' content and their secretory activity. Consequently, a significant decrease of glucose and Hb1c concentration and an increase of the level of insulin were observed in blood.These results afford to use the modulation of macrophage as a promising strategy in the stimulation of reparative processes in the pancreas.

Investigation of Some Pharmacological Effects of Caffeine and Taurine in Food Supplements

In modern times in all age groups energy supplements containing different amounts of Caffeine and Taurine are applied. Caffeine is purine alkaloid, which stimulates central nervous system action, enhances the strength and frequency of the cardiac contractions and increases the excretion of urine. Taurine is a sulfur containing amino acid, which possesses many fundamental biological roles including: effect on synaptic transmission in the central nervous system, cardiotropic action, antioxidant and anticonvulsant activity, improvement of energy processes, stimulation of reparative processes in tissues, protection of eyes cataract, decrease of cholesterol and stimulation of immune system. The combination of Caffeine and Taurine provide benefit due to obtaining synergism of pharmacological effects in increasing of physical activity, stimulation of brain action, cognition, memory and attention. In connection with the significant enlarging of the consumption of energy drinks, especially by children and young people in recent years the requirements for regulation and control of the labeling of these products in many countries are enlarged. In many food additives Caffeine and Taurine are added in not labeled high concentrations, which can provoke and increase their side effects. High consumption of Caffeine enhances its adverse effects on body: anxiety, headache, insomnia, nervousness, respiratory disorders, tachycardia, tremor, dehydration. In children the adverse reactions of Caffeine in much lower doses than adults are occurred. In high concentrations Taurine has adverse effects on brain activity and can induce psoriasis. The result of combination of Caffeine and Taurine is associated with increased diuretic effect and loss of water and salts from the body, especially in children and young people. Because of these facts the quality and quantity control of included compounds in food supplements is important for their health safety.

Multiplex Analysis of Cytokines, Chemokines, Growth Factors, MMP-9 and TIMP-1 Produced by Human Bone Marrow, Adipose Tissue, and Placental Mesenchymal Stromal Cells

Comparative analysis of mesenchymal stromal cells isolated from human BM, adipose tissue, and placenta was carried out. The cells were compared by the levels of constitutive, spontaneous, and LPS-induced production of Th1/proinflammatory (IFN-γ, IL-2, IL-1β, TNF-α, IL-12, IL-17) and Th2/anti-inflammatory cytokines (IL-4, IL-5, IL-6, IL-10, IL-13), chemokines (IL-8, MCP-1, MIP-1β), growth factors (IL-7, granulocytic CSF, granulocytic macrophageal CSF, erythropoietin, VEGF, EGF, IGF-1, main FGF), matrix metalloproteinase-9, and tissue inhibitor of metalloproteinase-1. Mesenchymal stromal cells originating from different tissues were characterized by functional potential for hemopoiesis support (through production of granulocytic CSF, granulocytic macrophage CSF, erythropoietin), immunomodulation (through production of IFN-γ, IL-2, IL-6, IL-1β, TNF-α and chemokines IL-8, MCP-1, MIP-1β), and stimulation of reparative processes (through production of VEGF, FGF, IGF-1, IL-6 tissue inhibitor of metalloproteinase-1, and matrix metalloproteinase-9). By the type and levels of spontaneous (basal) production of cytokines, the adipose tissue mesenchymal stromal cells more distinctly demonstrated the proinflammatory (IL-1β TNF-α), immunoregulatory (IFN-γ, IL-2, IL-4, IL-6, IL-8, MCP-1, MIP-1β), and hemopoiesis-stimulating (granulocytic CSF, granulocytic macrophage CSF) phenotype and at the same time were characterized by lower sensitivity to lipopolysaccharide stimulation than BM and placental mesenchymal cells.

Study of Anti-Ischemic Effect of Afobazole in Experimental Myocardial Infarction

Seven-day treatment of rats with experimental myocardial infarction with afobazole (5-ethoxy-2-[2-morpholino)-ethylthio] benzimidasole dihydrochloride) resulted in shrinkage of the ischemic damage area in the heart, stimulation of reparative processes in the myocardium, and prevention of postinfarction remodeling of the left ventricle. Anti-ischemic effect of afobazole in experimental myocardial infarction is presumably due to its interactions with σ(1) receptors.

Wound model of myocardial infarction

ischemic heart disease is the major etiology for heart failure today ([3][1]). The initial event is frequently a large or recurrent myocardial infarction. Acute myocardial infarction starts with thrombotic occlusion of a coronary artery, develops during several hours, and terminates when necrosis

Healing of bone fractures of rat shin and some immunological indices during magnetic laser therapy and osteosynthesis by the ilizarov method.

The effect of magnetic and laser therapy on healing of bone fractures and blood levels of T and B lymphocytes was studied in rats during osteosynthesis by the Ilizarov method. Laser therapy induced changes in cells attesting to stimulation of reparative processes and normalization of immunological parameters.

Stimulation of reparative processes in inflammatory-destructive diseases of the periodontium

The effect of direct current (5–12 μA) induced by a bimetallic pin inserted into the pulp canal on cellular and vascular reactions is studied in a model of chronic apical periodontitis. It is demonstrated that after 90 days the inflammatory reaction decreases, osteogenesis is activated, and macrophagal infiltrate is observed only at the root apex as a mature granuloma with the signs of reduction.