Sterilization Treatment Research Articles

Development of in vitro culture establishment conditions and micropropagation of grapevine rootstock cultivar ‘Ruggeri-140’

Optimization of in vitro culture conditions of grapevine phylloxera-resistant rootstock cultivar ‘Ruggeri-140’(Vitisberlandieri x Vitisrupestris) was carried out. Among the different sterilization treatments, maximum aseptic cultures were obtained for both explants apical tips and nodal segments when treated with Ca(ClO)2 at concentration of 1.5 % for 10 minutes plus 70 % ethanol for 30 s (T7). The maximum shoot proliferation was observed both in apical and nodal meristems cultured on MS medium supplemented with 1.0 mg/l BAP. MS/2 medium containing 1.0 mg/l indole-3-butric acid (IBA) gave the highest rooting percentage (100%) with the highest mean number and length of roots. The ex vitro survival of rooted micro shoots was 75.0%.

Influence of AM fungi inoculation on Capsicum annuum L. plant grown in microwave-sterilized media

The presence of arbuscular mycorrhizal in soil may affect growth and yield of chili (Capsicum annuum L.). This experiment was done to know the effect of arbuscular mycorrhizal inoculation on growth of chilli. Microwave soil sterilization was used to reduce the number of microbes in the media, enabling to observe the interaction between chili peppers and arbuscular mycorrhizal fungi. A single culture products (A) and mixed culture products (B) were used as arbuscular mycorrhizal spores. In contrast to product A, the spore counted calculation reported that product B had the most spores, with 51 spores / 50 g soil. The treatment of arbuscular mycorrhizal fungi and microwave sterilization against the height of chili plant had no significant effect, according to a two-factor ANOVA (α: 0.05) analysis of agronomic characteristics. Inoculation of mycorrhizae had a significant effect on chili plant height. Arbuscular mycorrhizal fungi inoculation and microwave sterilization had significant effect on the root length of chili plants. Arbuscular mycorrhizal fungi in single and mixed cultures could colonize roots by forming internal hyphae, vesicles, and spores. The best way to support the growth of chili plants is to use planting media that has not been sterilized and contains mycorrhizal fungi.

Remote Online Two-Step Stress Lifetime Acceleration Test System for Ultraviolet Light-Emitting Diodes

Ultraviolet light-emitting diodes (UV LEDs) have been widely applied in various fields, such as sewage treatment, sterilization, and disinfection medical treatment. However, the remained problems of UV LEDs mainly concern poor photoelectric stability, low efficiency, safety protection, and among others. In order to evaluate and predict the stability and lifetime of UV LEDs safely and efficiently, a comprehensive UV LEDs two-step aging test system has been designed in this work. Two steps of different temperature stresses with total aging time up to 720 h are applied to UV LEDs. The junction temperature derived from spectroscopy has been taken under consideration during the test to evaluate lifetime accurately. A smartphone-based remote control and test scheme has been developed to monitor and control the online testing and therefore ensure the safety and convenience during the aging experiment.

Study and Proposal for a Hyperfluorinated Brackish Water Treatment System in the Fatick Region, Case of Diouroup (Senegal)

Groundwater is the main resource for human consumption in many countries, especially in developing countries. This groundwater is often brackish and hyperfluorinated, which leads to diseases such as dental and bone fluorosis, etc. The water from the Diouroup water drainage facilities, like those from many other water drainage facilities in the regions of Fatick, Kaolack, Diourbel and the area of Touba, is facing this problem. To solve these problems, several physicochemical and membrane methods have been implemented. In this work we have briefly outlined some of these methods and we have chosen one of them, low pressure reverse osmosis. In addition, this technique is very simple to operate and maintain. Reverse osmosis provides good quality water in a single step, without the need for additional sterilization or remineralization treatments. We then carried out simulations with the Reverse Osmosis System Analysis (ROSA) software. For reasonable operating parameters, we have noticed a low feed pressure of 11.58 bars, a good average flow of raw water of 27.79 L/m2/h and a recovery rate of the first pass of 75.01%. The results obtained also showed a good quality of the permeate which respects the recommendations of the World Health Organization (WHO) on drinking water. The fluorides of 0.59 mg/L have a recovery rate of 90.8% while the chlorides of 59.09 mg/L have a recovery rate of 92.12% as for the Total Dissolved Solids of 184.90 mg/L for an abatement of 92%. Finally, the low energy consumption of the process makes it possible to consider it in coupling with an electric supply by photovoltaic solar collectors for isolated sites.

Pre- and postharvest factors affecting quality and safety of Pepper ( Piper nigrum L.)

Abstract The quality and safety of pepper ( Piper nigrum L.) are a function of crop management practices, harvest maturity, harvesting methods and subsequent handling after harvest, including storage conditions and processing methods. The review shows that volatile oil decreases with shade intensity (15–30%). Essential oil, oleoresin, piperine and monoterpenes (thujene, α-pinene, sabinene, limonene, α-phellandrene and linalool) increase with altitude, while β-caryophyllene and total phenol decrease with increase in altitude. Fermentation of ripe pepper fruits forms odorants (butanoic acid, 3-methylindole, and 4-methylphenol) attributing intense faecal/shed-like off-flavour white pepper powder. Low-drying temperature (≤56°C) and duration (≤3 hrs) lead to low bulk density (0.17 g/ml) and low moisture loss (31%). Excessive soaking of black peppercorns prior to mechanical decortication reduces volatile oil and increases moisture content and broken berries. Availability of heavy metals in grinding machines increases the level of heavy metals; Fe (69.8–1147 mg/kg), Pb (21.3–947 μg/kg) and V (64.1–1072 μg/kg) in pepper powder. Storing peppercorns along with other materials enhances cross-contamination of heavy metals; Pb, Cd and Cr. High moisture content (>13%) and farmers’ unawareness resulted in high (2200 to >30000 cfu/g) mycotoxins contamination. Environmental and industrial pollutants such as plasticizers, bisphenol A, polycyclic aromatic hydrocarbons and pesticides are also pepper contaminants of high merit. Radiofrequency pasteurization, vacuum-assisted steaming, ethylene oxide fumigation, atmospheric pressure plasma, dry heat sterilization, gamma-irradiation and ultraviolet-C light treatments are among strategies to enhance the quality and safety of pepper. More precautions also have to be taken to regulate the shade, drying temperature and duration, soaking duration, hygienic processing and storage in order to retain quality, minimize the risk of microbial or chemical contaminations and comply with standards.

Investigation on Continuous and Modulated Microwave Plasma Filaments at Atmospheric Pressure

Microwave atmospheric pressure plasma jets continue to evolve with the growth of atmospheric plasma’s applications in sterilization, material modification, and cancer treatment. In this article, continuous and modulated microwave plasma filaments were produced by a hand-held microwave plasma jet, and then they were compared in plasma length, electron temperature, and emission spectrum. The electron temperature was measured by the emission spectroscopy diagnostic method, and the regularities of the compared parameters’ change were studied by varying duty cycles and periods of the modulating signal. The results prove that the modulated microwave can generate stable plasma filaments directly in the air without protective tubes, and it is different from the continuous microwave plasma in all compared parameters. Besides, stable dendritic tearing of plasma filaments was observed. These results imply that modulated microwave and continuous microwave plasma filaments cannot be regarded as the same, the changing manner and the reaction on the surface of the medium may be completely different even if they are the same to the naked eye in most cases. These results are helpful for the controlling and understanding of microwave plasma, and the stable tearing could be used to expand the treated area of a single plasma jet.

Identification and Analysis of <I>PGM</i> and <I>SUS</i> Gene Families in Leymus Chinensis Seed Transcriptome

Leymus chinensis ( Leymus chinensis  (Trin.) Tzvel.) is a good perennial forage with good palatability, high yield and high feeding value. In addition, because of its strong resistance, Leymus chinensis can improve the disadvantaged environment such as poor land, and it is an important species to protect grassland ecology in China. Therefore, in order to promote the germination of leymus chinensis seeds and explore the mechanism of Germination, this study used sterile water treatment of leymus chinensis seeds as control, exogenous GA3 as treatment conditions. Based on the analysis of pre-transcriptome data, it was determined that  PGM  and  SUS  genes in sucrose and starch metabolic pathways play a significant role in seed Germination. Members identification, conserved domains, and evolutionary trees were analyzed for both gene families using the bioinformatics method. The expression pattern of  PGM  and  SUS  genes were analyzed with FPKM value. The results showed that 7 members were selected from each of the the  PGM  and  SUS  families based on the data of Leymus Chinensis seeds transcriptome. The molecular weight of  PGM  genes ranged from 15 471.3 to 67 912.9 Da, and the isoelectric point value ranged from 4.45 to 6.31, showing weak acidity.  PGM  family in Leymus Chinensis seeds are hydrophilic stable proteins. The molecular weight of  SUS  genes ranged from 30 421.5 to 110 262.9 Da. The isoelectric point of  SUS  genes were neutral except  LcSUS1,5  and  7 , while the other four genes were weak acidic.  LcSUS4 , 6  were stable hydrophilic proteins, the rest were unstable hydrophilic proteins. The  PGM and  SUS  gene families in Leymus chinensis seeds play an enzyme role in promoting sucrose and starch synthesis in sucrose and starch metabolism pathways. The expression pattern analysis of Leymus chinensis seeds showed that the genes of  PGM  and  SUS  gene families in GA3-treated Leymus chinensis seeds promoted the germination of Leymus chinensis seeds by regulating the sucrose and starch pathways with different expression levels.

Research on key techniques of tissue culture of Qinghai Rhubarb

Rheum palmatum L. is a perennial herb of the Rheum genus Polygonaceae. It is a traditional Chinese herbal medicine which has extremely high medicinal value. Rhubarb has scarce seeds in subtropical regions, and natural planting conditions have limitations. In recent years, the demand of rhubarb has increased, and the means of sowing and raising seedlings can no longer meet the needs of the market. Traditional sowing and propagation methods have limitations such as long cycle and low reproduction coefficient. Tissue culture technology can shorten the reproduction cycle that increase the reproduction coefficient and maintain the excellent characteristics of the parents, which is an effective way to promote the large-scale and commercial production of fine varieties of rhubarb. The results of the study showed that: (1) Analysis of the characteristics of Qinghai Rhubarb seeds. (2) Seed sterilization treatment. (3) Primary culture medium selection. (4) Callus induction. (5) Callus proliferation culture. (6) Hypocotyl callus bud differentiation.(7)Rooting culture. (8) Qinghai Rhubarb tissue culture seedling transplantation. Using the method of the present invention to establish a Qinghai Rhubarb in vitro culture aseptic propagation system, high-quality Qinghai Rhubarb seeds as explants and different hormone medium ratios, Qinghai Rhubarb hypocotyl induction rate reached 90%, and the proliferation coefficient reached 4.3. The survival rate of transplanting tissue cultured seedlings reaches 95% which can improve the reproduction efficiency of Qinghai Rhubarb, and provide theoretical basis and technical support for subsequent research on transgenic and genetic improvement. It can also provide a basis for medicinal value for the systematic research and commercial production of Qinghai Rhubarb.

Surface sterilization and callus induction potential of various indica rice (Oryza sativa L.) Varieties

The present study was conducted to standardize the efficient and reliable surface sterilization and callus induction protocol for 22 rice (Oryza sativa L.) varieties developed by Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli, Maharashtra (India) using different combinations of various plant growth regulators. The dehusked mature rice seed was used as explant. Prior to callus induction, surface sterilization protocol was standardized using 70% ethanol and different concentrations of mercuric chloride (HgCl2). The results revealed that surface sterilization treatment with 70% ethanol for 1 min, followed by 1.0% HgCl2 for 10 min and then washing the seeds 4-5 times was most effective in establishing maximum aseptic cultures (86.67%). Callus induction potential differed significantly among the rice varieties for the concentrations of plant growth regulators in MS medium. Rice cv. ‘Ratnagiri-711’ followed by cv. ‘Karjat-8’ were most superior in callus induction and proliferation potential with the respective callus induction frequency of 87.56 and 87.0%, and callus weight of 269.37 and 235.20 mg after 45 days of inoculation. The supplementation of 2,4-D @ 1.0 to 2.0 mg L-1 along with 0.5 mg L-1 BAP in MS medium was found optimum for embryogenic callus formation. All the calli formed on MS medium supplemented with combinations of plant growth regulators were yellowish-white in colour but with different textures as compact, soft, friable that were embryogenic and mucilaginous that were non-embryogenic.

Effects of neighbors with different sexuality and soil sterilization on biomass of Populus cathayana seedlings

Although plant-plant interactions have been intensively studied, few studies examined sex-related neighbor interactions in dioecious plant species. Here, we investigated the sexual diffe-rences in biomass accumulation, and analyzed the sexual neighbor effects and yields of sexual combination in Populus cathayana seedlings under the treatments of inter- and intra-sex neighbors and soil sterilization. The results showed that biomass accumulation of P. cathayana decreased in both of sexes when grown with an intra-sex neighbor. Females increased biomass accumulation under non-sterilization condition when grown with an inter-sex neighbor. Soil sterilization decreased biomass accumulation in both sexes of these plants, especially for females. Soil sterilization treatments did not significantly affect intra-sex interactions, but neutralized the positive effects in females and led the effects in males to turn from neutral to negative in inter-sex neighbors. The highest biomass in female-male combination under non-sterilization condition and the lowest biomass in male-male combination under sterilization condition were observed among combinations. These results indicated that the growth of female and male P. cathayana differed in their responses to their sexual neighbors, and that such neighbor effects were affected by soil microorganism. Our results could contribute to improve the productivity of poplar plantations in silviculture.

Effects of Surface Sterilization Protocol and Haustorium Removal on Germination and Growth of Hybrid PB121 Coconut Palm (Cocos nucifera L.) Zygotic Embryos Cultured in vitro

Aims: This study aims to identify the best surface sterilization and evaluate the effect of haustorium suppression on in vitro germination of coconut palm (Cocos nucifera L.) zygotic embryos.
 Study Design: Survival rate and contamination rate of zygotic embryos after different surface sterilization treatments, regeneration rate and organogenesis through the number of leaves and the length of shoots after haustorium suppression were determined. For data processing, the Analysis of Variance was used to compare the means which were separated according to Tukey test (P = 0.05).
 Place and Duration of Study: Coconut fruits (hybrid PB121) were collected 12 to 14 months after controlled pollination from CRAPP (Centre de Recherches Agricoles Plantes Pérennes), station of Sèmè-kpodji in Benin. Experiments were done in Central Laboratory of Plant Biotechnology and Plant Improvement, University of Abomey-Calavi and conducted from june to december in 2019.
 Methodology: For the zygotic embryos surface sterilization, four treatments combining three concentrations (3%, 6% and 15%) of commercial bleach (Javel la Croix© containing 12° active chlorine) and immersion durations (5 min, 10 min and 20 min) were tested and the survival rate were determined for each treatment after two months culture. The zygotic embryos were then divided in two sets (haustorium excised embryos set and the whole embryos set) and cultured in modified Y3 medium supplemented with 7 g L-1 agar, 2.5 g L-1 activated charcoal, 5% sucrose, 6.10-3 mM 2.4 D (2.4-dichlorophonoxyacetic acid), gibberellic acid and 0.3 mM BAP(6-benzylaminopurine). After five months culture, the regeneration rate, the number of leaves and the length of shouts were recorded.
 Results: The high survival rate (80%) was obtained with 6% of bleach and 20 min for the immersion duration without pre-disinfection. The suppression of haustorium have significantly increased the number of leaves (4.3 ± 0.02) and the length of shoots (16.2 ±0.7cm) compared to the whole zygotic embryos.
 Conclusion: This protocol can help to ensure better surface sterilization of zygotic embryos before their in vitro culture and the development of vigorous plantlets in order to improve the slow growth of plantlets, when transferred to the greenhouse or field.

Comparative study on immunoreactions of small intestinal submucosa by irradiation and ethylene oxide sterilization treatments

Comparative study on immunoreactions of small intestinal submucosa by irradiation and ethylene oxide sterilization treatments

Comparison and analysis of several medical waste treatment technologies

Medical waste is a kind of special waste with great harm. Since the 1950s, the treatment of medical waste has attracted widespread attention from all over the world. At present, scholars at home and abroad are increasingly rich in research on medical waste treatment technology and have achieved certain research results. This article comprehensively compares and analyzes the technical influencing parameters, advantages and disadvantages of incineration treatment, pressure steam sterilization treatment, chemical treatment, microwave treatment, plasma treatment and other medical waste treatment methods, and emphasizes that high-temperature pyrolysis incineration treatment technology has reduced weight and is harmless. This is the current direction of domestic medical waste treatment research and development.

High resolution microscopy to evaluate the efficiency of surface sterilization of Zea Mays seeds.

Surface sterilization of seeds is a key step in providing microorganisms-free seeds for numerous applications like understanding the role of seed-borne microorganisms in plant development, studying microbial cells-plant interactions by inoculating model microorganisms in a simplified system or selective cultivation of seed endobionts. However applying efficient treatment for surface sterilization of seeds without affecting the plant growth is not an easy task. In this study we aimed to provide an efficient surface sterilization treatment for maize seeds using i) hydrogen peroxide (HP), ii) sodium hypochlorite (SH) and iii) ethanol-sodium hypochlorite (EtOH-SH) under stirring (st) and vacuum-stirring (va-st) conditions. We used fluorescence microscopy and ultra-high resolution Helium Ion Microscopy (HIM) as powerful imaging approaches in combination with macroscopic techniques to visualize, quantify and evaluate the efficiency of seed sterilization, quality of root germination, seedlings and root hair development as well as the presence or absence of microorganisms on the root surface. Our results showed a strong reduction in microbial cell numbers of 4 orders of magnitude after the EtOH-SH treatments. Moreover, seeds exposed to EtOH-SH treatments displayed the lowest percentage of microbial growth (50%) and the highest percentage of germinated seeds (100%) compared to other sterilization treatments. HIM imaging proved the absence of microbial cells on the roots grown from seeds exposed to EtOH-SH treatments. Moreover, root hair development seemed not to be affected by any of the sterilization treatments. Our findings demonstrated that EtOH-SH treatments are significantly reducing the abundance of microbial cells from the surface of maize seeds and can be used with high confidence in future studies.

Effects of Microbes from Coal-Related Commercial Humic Substances on Hydroponic Crop Cultivation: A Microbiological View for Agronomical Use of Humic Substances.

Here, coal-related humic substances (HSs) were examined to confirm whether sterilization treatments induce their inferior ability to stimulate lettuce in hydroponic cultivations. Interestingly, a drastic reduction in both lettuce biomass and microbial colony-forming units of the crop culture solutions was observed when the autoclaved HSs were treated. Some microbial genera (i.e., Bacillus and Aspergillus) identifiable in the bare HS-treated hydroponic systems were able to be isolated by direct inoculation of bare HS powders on conventional microbial nutrients, supporting that flourishing microbes in the hydroponic cultivations derive from bare HSs-treated. Moreover, coincubation of some isolated bacterial and fungal strains (i.e., Bacillus and Aspergillus genera) from HSs with lettuce resulted in a significant increase in plant biomass and enhanced resistance to NaCl-related abiotic stresses. Microbial volatile organic compounds renowned for plant stimulation were detected by using solid-phase microextraction coupled with gas chromatography-mass spectrometry. It was finally confirmed that the isolates are capable of utilizing carbon substrates such as pectin and tween 20 or 40, which are relevant to those of microbes isolated from peat and leonardite (i.e., HS extraction sources). Overall, our results suggest that microbiological factors could be considered when commercial coal-related HSs are applied in hydroponic crop cultivations.

Abiotic conditions outweigh microbial origin during bacterial assembly in soils.

Understanding the processes guiding microbial community assembly in soils is essential for predicting microbiome structure and function following soil disturbance events like agricultural soil fumigation. However, assembly outcomes are complex and variable, being affected by both selective abiotic forces and by the history of colonizing microorganisms. To untangle the interactions between these factors, we conducted a controlled microcosm study tracking bacterial assembly in cleared soils over 7 weeks. We used mesh bags to connect five unsterilized source soils, differing in land use history (forested, agricultural, or fallow), with four sterile recipient soil treatments, differing in abiotic conditions (no soil additives, salt addition, urea addition, or mixed salt/urea addition). We found that 59%-96% of bacterial colonizers after 1 week were Firmicutes, but by 7 weeks Actinobacteria and Bacteroidetes were also dominant. Salt and nitrogen additions reshaped bacterial assembly by constraining alpha diversity by up to half and biomass accumulation by up to an order of magnitude. Within-treatment dispersion was significantly lower for salt and nutrient addition microcosms, suggesting deterministic selective pressures. In contrast, source soil origin had little impact on assembly trajectories. These results suggest that abiotic conditions can overshadow microbial source history in shaping community assembly outcomes.

High voltage atmospheric cold plasma treatment inactivates Aspergillus flavus spores and deoxynivalenol toxin

Fungal contamination is a concern for the food industry. Fungal spores resist food sterilization treatments and produce mycotoxins that are toxic for animals and humans. Technologies that deactivate spores and toxins without impacting food quality are desirable. This study demonstrates the efficiency of a high voltage atmospheric cold plasma (HVACP) technology using air to generate reactive oxygen (ROS) and nitrogen (RNS) species for the degradation of Aspergillus flavus cultures and the deoxynivalenol (DON) mycotoxin. Optical emission and absorption spectroscopy demonstrate ionization of hydroxyl groups, atomic oxygen and nitrogen, and confirm production of ROS and RNS, e.g. O3, NO2, NO3, N2O4, and N2O5. Fungal cultures show a depletion in pigmentation and an ~50% spore inactivation after 1-min treatments. Treated spores show surface ablation and membrane degradation by scanning electron microscopy. Twenty-minute direct HVACP treatments of 100 μg of DON in one mL aqueous suspensions resulted in a greater than 99% reduction in DON structure and rescued over 80% of Caco-2 cell viability; however, the same treatment on 100 μg of powdered DON toxin only showed a 33% reduction in DON and only rescued 15% of cell viability. In summary, HVACP air treatment can inactivate both fungal spores and toxins in minutes.

Heritable Variation in Pea for Resistance Against a Root Rot Complex and Its Characterization by Amplicon Sequencing.

Soil-borne pathogens cause severe root rot of pea (Pisum sativum L.) and are a major constraint to pea cultivation worldwide. Resistance against individual pathogen species is often ineffective in the field where multiple pathogens form a pea root rot complex (PRRC) and conjointly infect pea plants. On the other hand, various beneficial plant-microbe interactions are known that offer opportunities to strengthen plant health. To account for the whole rhizosphere microbiome in the assessment of root rot resistance in pea, an infested soil-based resistance screening assay was established. The infested soil originated from a field that showed severe pea root rot in the past. Initially, amplicon sequencing was employed to characterize the fungal microbiome of diseased pea roots grown in the infested soil. The amplicon sequencing evidenced a diverse fungal community in the roots including pea pathogens Fusarium oxysporum, F. solani, Didymella sp., and Rhizoctonia solani and antagonists such as Clonostachys rosea and several mycorrhizal species. The screening system allowed for a reproducible assessment of disease parameters among 261 pea cultivars, breeding lines, and landraces grown for 21 days under controlled conditions. A sterile soil control treatment was used to calculate relative shoot and root biomass in order to compare growth performance of pea lines with highly different growth morphologies. Broad sense heritability was calculated from linear mixed model estimated variance components for all traits. Emergence on the infested soil showed high (H2 = 0.89), root rot index (H2 = 0.43), and relative shoot dry weight (H2 = 0.51) medium heritability. The resistance screening allowed for a reproducible distinction between PRRC susceptible and resistant pea lines. The combined assessment of root rot index and relative shoot dry weight allowed to identify resistant (low root rot index) and tolerant pea lines (low relative shoot dry weight at moderate to high root rot index). We conclude that relative shoot dry weight is a valuable trait to select disease tolerant pea lines. Subsequently, the resistance ranking was verified in an on-farm experiment with a subset of pea lines. We found a significant correlation (rs = 0.73, p = 0.03) between the controlled conditions and the resistance ranking in a field with high PRRC infestation. The screening system allows to predict PRRC resistance for a given field site and offers a tool for selection at the seedling stage in breeding nurseries. Using the complexity of the infested field soil, the screening system provides opportunities to study plant resistance in the light of diverse plant-microbe interactions occurring in the rhizosphere.

Physicochemical properties of Ocimum sanctum enriched herbal fruit yoghurt

Fruit yoghurt with tulsi enhance the nutritional value and satisfy rising demands for healthy food products. Three different concentrations of TLE (0.5%, 1%, and 1.5%); and apple pulp (10%) were used for the formulation of herbal fruit yoghurt. The enriched milk (26.42–28.58 min) showed lower heat stability than that of control (29.80 min), however, sufficient to withstand sterilization treatment up to 1% TLE addition. Furthermore, physicochemical properties viz. pH, water holding capacity, acidity, moisture, water activity, setting time, and whey-off experiment were not affected significantly (p > .05) in comparison to control yoghurt. The tulsi addition significantly (p < .05) increased (from 32.43% to 45.47% inhibition) the antioxidant activity of yoghurt. The firmness of herbal fruit yoghurt was decreased than control yoghurt. Sensory scores were decreased with an increase in the concentration of TLE (88.75 to 85.14) in comparison to control (91.96), however, yoghurt was suitable for consumption up to 1% TLE. Practical applications Dahi, Tulsi, and fruit mix are considered as religious food and known as Panchamrite. Yoghurt is considered as western dahi in India, however, no such mixture is tried with yoghurt. Therefore, the feasibility of yoghurt with tulsi and apple was carried out, as yoghurt has higher health benefits even than tulsi. A good quality yoghurt was developed using tulsi and apple pulp, therefore, it will capture a huge place in the Indian market.

Triple stimulation (TriStim) before bilateral oophorectomy in a young woman with ovarian cancer: a case report and review of the literature

BackgroundDouble ovarian stimulation (DuoStim) involves two rounds of controlled ovarian stimulation (COS) and oocyte retrieval in immediate succession. It represents a promising approach to increase oocyte yield for patients with diminished ovarian reserve or those with limited time before fertility-threatening oncologic treatment. We report the case of a 31-year-old woman with Stage IC endometrioid ovarian cancer who underwent a triple stimulation or “TriStim,” completing three rounds of COS and oocyte retrieval within 42 days prior to bilateral salpingo-oophorectomy.Case presentationA 31 year old nulligravid woman presented for fertility preservation counseling following a bilateral ovarian cystectomy that revealed Stage IC endometroid adenocarcinoma arising within endometrioid borderline tumors. The patient was counseled for bilateral salpingo-oophorectomy, lymph node dissection, and omentectomy followed by three cycles of carboplatin/paclitaxel. Prior to this, all within six weeks, the patient underwent three rounds of controlled ovarian stimulation using an antagonist protocol and human chorionic gonadotropin (hCG) trigger, resulting in vitrification of nine two-pronuclear zygotes (2PN), after which definitive surgery was performed.ConclusionsAdvantages of DuoStim procedures are increasingly recognized, especially for oncology patients with limited time before potentially sterilizing cancer treatment. To our knowledge, this is the first report of a triple stimulation (“TriStim”). Our case highlights that triple stimulation is a viable option for patients needing urgent fertility preservation in order to maximize egg and embryo yield within a limited time period.