Development of in vitro culture establishment conditions and micropropagation of grapevine rootstock cultivar ‘Ruggeri-140’

Gayane Melyan,Kima Dangyan,Yuri Martirosyan,Andranik Barsegyan,Narek Sahakyan

doi:10.1051/bioconf/20213903002

Abstract

Optimization of in vitro culture conditions of grapevine phylloxera-resistant rootstock cultivar ‘Ruggeri-140’(Vitisberlandieri x Vitisrupestris) was carried out. Among the different sterilization treatments, maximum aseptic cultures were obtained for both explants apical tips and nodal segments when treated with Ca(ClO)2 at concentration of 1.5 % for 10 minutes plus 70 % ethanol for 30 s (T7). The maximum shoot proliferation was observed both in apical and nodal meristems cultured on MS medium supplemented with 1.0 mg/l BAP. MS/2 medium containing 1.0 mg/l indole-3-butric acid (IBA) gave the highest rooting percentage (100%) with the highest mean number and length of roots. The ex vitro survival of rooted micro shoots was 75.0%.

Highlights

Grapevine is one of the important crops grown in Armenia
Since not all cells in a shoot apical meristem are infected with pathogens, it is possible to dissect out a non-infected section and manipulate this explant in vitro to produce virus–free plants [5, 11]
Two different chemicals i.e. calcium hypochlorite and ethanol were used for the present study to standardize the best sterilization procedure for in vitro culture of phylloxeraresistant rootstock cultivarRuggeri-140

Summary

Introduction

Grapevine is one of the important crops grown in Armenia. Use of hardwood cuttings is the general way to propagate grapes. The viruses which are causing the diseases on grapes are transmitted by this type of propagation. The use of healthy planting material is a main factor in the prevention of disease development. The culture of meristem is the only way to obtain virus free biological material [7]. Plant pathogens, such as fungi, bacteria, viruses, viroids and nematodes can be transmitted from diseased to healthy plants. The shoot apical meristems are mainly virus free. Since not all cells in a shoot apical meristem are infected with pathogens, it is possible to dissect out a non-infected section and manipulate this explant in vitro to produce virus–free plants [5, 11]

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Conclusion