Lycopene is an organic compound belonging to tetraterpene and a carotene category. Lycopene is a brick red carotenoid hydrocarbon found in tomatoes and other red fruits and vegetables. Lycopene is extracted from red fruit & vegetables and found in two physical form Oily liquid & granular solid powder form. Lycopene is also used as supplement with multivitamin formulated as tablets. An RP-HPLC method was developed and validated for the Lycopene (Powder form or water suspendable form) in Multivitamin Tablets. The chromatographic system was equipped with C18 a stainless steel column 30 cm x 4.0 mm, packed with octadecylsilane chemically bonded to porous silica or ceramic micro particles (5µm) and wavelength set at 475 nm, in conjunction with a mobile phase of Methanol, Water and Tetrahydrofuran in the ratio of 66:4:30 % v/v at a flow rate of 1.5 ml/min. The retention time of Lycopene was found to be 6 min ±1 min. The separation was performed at ambient temperature. Linearity was observed in the concentration range of 80-120% with correlation coefficient 0.9999 and slope 75145.63 Percentage recovery obtained 99.06-101.83 %. The percentage Assay was found to be 100.25 to 102.61 %.The proposed method is precise, accurate, selective and rapid for the determination of lycopene (Powder form) in Multivitamin tablet. The proposed method is optimized and validated as per guidelines of WHO TRS 937 & the International Conference on Harmonization (ICH) guidelines.