Standard BHA Research Articles

Phytochemical compounds and antiradical, antimicrobial, and cytotoxic activities of the extracts from Hypericum scabrum L. Flowers

Hypericum scabrum L. has been widely used in traditional medicine for the treatment of many diseases just as the other Hypericum species. In the present study, the antiradical, antimicrobial and cytotoxic activities of water and ethanol extracts of H. scabrum flowers were investigated. Their phytochemical contents and composition were also determined. The water and ethanol extracts are better scavenged ABTS (97.89 and 98.99%) and OH radicals (96.36 and 97.33%); the water extract is better scavenged DPPH radicals (91.66%) than the standard antioxidant BHA (94.33, 85.19, 90.16%, respectively). Flowers of H. scabrum contain flavonoids, phenolic acids, vitamins and phytosterols, dominated by catechin, vanillic acid, vitamin K and ergosterol. The extracts exhibit a strong cytotoxic activity against MCF-7, HCT-116, and LNCaP cancer cell lines. It is found that their antimicrobial activities are higher than the standard antibiotics. These results indicate that H. scabrum flowers have potent antiradical, antimicrobial and cytotoxic activities.

L-Proline-Catalyzed Synthesis of Phthalimide Derivatives and Evaluation of Their Antioxidant, Anti-Inflammatory, and Lipoxygenase Inhibition Activities

A study was planned to synthesize the phthalimide derivatives as phthalimides have versatile biological activities. To synthesize the phthalimide derivatives, initially the reaction was optimized with various catalysts, and L-proline was found to be the best catalyst as it provided excellent yield. A series of phthalimide derivatives was synthesized by facile one-top reaction of phthalic acid with aryl amines under mild reaction conditions in the presence of L-proline as catalyst. Products were obtained in excellent yields and structurally characterized by 1H, 13C NMR, and mass spectral data. Products 1–7 were evaluated for antioxidant, anti-inflammatory, and lipoxygenase enzyme inhibition activities. Compounds 1 and 4 showed potent antioxidant activity under DPPH with IC50 values 27.3 and 25.0 μM when compared with the standard BHA (IC50 = 44.2 μM), respectively. Compounds 1 and 4 further showed strong lipoxygenase inhibition activity with IC50 values 21.34 and 20.45 μM when compared with standard baicalein (IC50 = 22.60 μM), respectively. Compound 2 was found to be promising and about equal to the used standard aspirin in the inhibition of bovine serum albumin denaturation, while other compounds showed weak-to-moderate % inhibition.

Nema

Biological activities of Sorbus aucuparia L. leaves extract was tested in our study. Antineurodegenerative activity was evaluated by acetylcholinesterase (AChE) and tyrosinase (TYR) inhibitory activity assays while antioxidant activity was by tested using four different assays. In all antioxidant test we noticed concentration dependent activity of tested extract and the highest applied concentration of 500 μg/ml was the most active. Extract applied in concentration of 200 μg/ml showed higher percent of inhibition in DPPH test compared to both applied concentrations of standard substances BHA and BHT (50 and 100 μg/ml). In ABTS test, activity of extract applied in 500 μg/ml was comparable or higher then BHA and BHT/Vitamin C, respectively. Moreover, all concentrations of tested extract were significantly more active in s -carotene test than vitamin C. The lowest concentration of extract (100 μg/ml) was the most active in TYR assay reaching 42.57 % of the inhibition while in AChE assay there was not statistically significant differences among all applied concentrations.

Chemical Composition, Antioxidant and α-Glucosidase-Inhibiting Activities of the Aqueous and Hydroethanolic Extracts of Vaccinium myrtillus Leaves.

Vaccinium myrtillus (bilberry) leaf is traditionally used in southeastern Europe for the treatment of diabetes. In the present study, the ability of bilberry leaf extracts to inhibit carbohydrate-hydrolyzing enzymes and restore glutathione concentration in Hep G2 cells subjected to glucose-induced oxidative stress was investigated. A comprehensive analysis of the antioxidant activity of two bilberry leaf extracts was performed. The aqueous extract showed excellent total antioxidant and chelating activity. Its antioxidant activity in the β-carotene-linoleic acid assay was very good, reaching the activity of the antioxidant standard BHA (93.4 ± 2.3% vs. 95.1 ± 2.4%, respectively). The hydroethanolic extract (ethanol/H2O, 8:2, v/v), on the other hand, was a better radical scavenger and Fe2+ reducing agent. Furthermore, the aqueous extract was able to efficiently increase glutathione concentration in Hep G2 cells subjected to glucose-induced oxidative stress and restore it to the levels observed in non-hyperglycaemic cells. The hydroethanolic extract strongly inhibited α-glucosidase, with the IC50 statistically equal to the antidiabetic drug acarbose (0.29 ± 0.02 mg/mL vs. 0.50 ± 0.01 mg/mL, respectively). Phytochemical analysis revealed the presence of quercetin and kaemferol derivatives, as well as chlorogenic and p-coumaric acid. The study results indicate that V. myrtillus leaf may have promising properties as a supporting therapy for diabetes.

Antioxidant and antineurodegenerative properties of st. John's-wort dry extract

Different biological activities of Hypericum perforatum L. dry extract was tested in this study. Antioxidant activity was evaluated by four different assays. Antineurodegenerative activity was tested using acetylcholinesterase (AChE) and tyrosinase inhibitory activity assays. In all four antioxidant assays, concentration dependent manner was noticed in the activity of tested extract. The most active was the highest applied concentration (500 μg/ml). In DPPH test, extract applied in dose of 200 μg/ml showed similar or higher radical scavenging activity compared to the dose of 100 μg/ml applied for standard substances BHA and BHT. The lowest antioxidant activity for the extract was detected in ABTS test. All applied concentrations of dry extract were significantly more potent than vitamin C in s -carotene test. Moreover, the extract reached 40.31 and 45.56 % of the inhibition in AChE and TYR assays, respectively. In both cases, the most active concentration of the extract was 200 µg/ml.

Synthesis, characterization and biological studies of Schiff bases derived from heterocyclic moiety

Some new Schiff bases (H1-H7) have been synthesized by the condensation of 2-aminophenol, 2-amino-4-nitrophenol, 2-amino-4-methylphenol, 2-amino benzimidazole with thiophene-2-carboxaldehyde and pyrrole-2-carboxaldehyde. The structures of newly synthesized compounds were characterized by elemental analysis, FT-IR, 1H NMR, UV–VIS, and single crystal X-ray crystallography. The in vitro antibacterial activity of the synthesized compounds has been tested against Salmonella typhi, Bacillus coagulans, Bacillus pumills, Escherichia coli, Bacillus circulans, Pseudomonas, Clostridium and Klebsilla pneumonia by disk diffusion method. The quantitative antimicrobial activity of the test compounds was evaluated using Resazurin based Microtiter Dilution Assay. Ampicillin was used as standard antibiotics. Schiff bases individually exhibited varying degrees of inhibitory effects on the growth of the tested bacterial species. The antioxidant activity of the synthesized compounds was determined by the 1,1-diphenyl-2-picrylhydrazyl(DPPH) method. IC50 value of synthesized Schiff bases were calculated and compared with standard BHA.

Cytoprotective Properties of Antioxidant Protein from Curry leaves (Murraya koenigii L.) against Oxidative Stress Induced Damage in Human Erythrocytes

Oxidative stress induced by reactive oxygen species (ROS) cause lipid peroxidation at the human erythrocyte membrane with subsequent alterations in the ATPases function. In this study, potential cytoprotective activities of previously reported ”Antioxidant Protein from Curry leaves (APC)’’ in erythrocytes against reactive oxygen (ROS) species generated by pro-oxidants in vitro. The APC prevented red blood cell lysis induced by pro-oxidants; Fe: As (2:20 μmole), Hydrogen peroxide (0.2 mM), and tertiary butyl hydroperoxide (1 mM) upto 97.5, 82.5 and 63%, respectively. Further, APC prevented Fe:As induced K+ leakage in red blood cells up to 95%. The inhibition offered by APC on K+ leakage was comparable to inhibition offered by quinine sulphate, a known K+ channel blocker. Interestingly, the APC at dose dependently restored Na+K+ ATPase and Ca2+Mg2+ ATPase activities of erythrocyte membrane when altered by ROS. The restoration of ATPase activity by APC was two times more than standard antioxidants BHA and α-tocopherol. In conclusion, Curry leaves protein antioxidant is an effective antioxidant in preventing membrane damage and associated functions mediated by reactive oxygen species. It can be further developed as an effective bioprotective antioxidant agent to cellular components.

Synthesis and biological activities of 2-aminophenol-based Schiff bases and their structure–activity relationship

Six Schiff bases 8–13 were synthesized by condensation of 2-aminophenol (1) with various chloro- and nitro-benzaldehydes (2–7), characterized, and evaluated for antioxidant, antibacterial, lipoxygenase, and urease inhibitory potentials. All Schiff bases except 11 showed potent antioxidant activity with IC50 values ranging between 17.2 and 33.1 μM when compared with standard BHA (IC50 = 44.2 μM); especially, compound 10 (IC50 = 17.2 μM) and compound 13 (IC50 = 18.6 μM) exhibited much better potential than the standard BHA. In lipoxygenase inhibition activity, all products except 8 showed excellent-to-good potential with IC50 values ranging from 22.5 to 110.5 μM while comparing with standard baicalein (IC50 = 22.6 μM). Compound 10 (IC50 = 22.5 μM) showed lipoxygenase potential comparable to the standard baicalein (IC50 = 22.6 μM), and molecular docking simulations showed that nitro group of 10 penetrated deeply into a pocket surrounded by basic amino acid residues which revealed the excellent activity of 10. All products also showed good potential against all gram-positive S. intermedius, B. subtilis, and S. aureus, and moderate activity against gram-negative E. coli and S. typhi bacteria. However, the products showed nonsignificant inhibitory potential against urease enzyme.

Protective Effect of Sundakai (Solanum torvum) Seed Protein (SP) Against Oxidative Membrane Damage in Human Erythrocytes.

Lipid peroxidation by ROS at the membrane level disturbs the inherit integrity of components activating subsequent alterations in the function. In this study, the protective effect of purified Sundakai (Solanum torvum) seed protein (SP) was tested against oxidative membrane damage in erythrocyte membrane. SP prevented oxidative RBC lysis induced by pro-oxidants; Fe:As (2:20 μmol), periodate (0.4 mM), and t-BOOH (1 mM) up to 86, 81, and 86 %, respectively. Further, SP prevented the Fe:As-induced K(+) leakage up to the tune of 95 %. The inhibition offered by SP on K(+) leakage was comparable to inhibition offered by quinine sulfate, a known K(+) channel blocker. SP dose dependently restored Na(+)K(+) ATPase and Ca(2+)Mg(2+) ATPase activities in erythrocyte membrane. The restoration of ATPase activity by SP was two times more than standard antioxidants BHA and α-tocopherol. Besides, SP at 1.6 μmol restored the membrane proteins over Fe:As induction when analyzed by SDS-PAGE, which was comparable to protection offered by BHA. In conclusion, SP is an effective antioxidant in preventing oxidative membrane damage and associated functions mediated by ROS. As SP is non-toxic, it can be used as an effective bioprotective antioxidant agent to cellular components.

Flavonoids and other bioactive constituents from Ficus thonningii Blume (Moraceae)

Continued interest in the chemistry of Ficus spp. led to the investigation of the figs and the roots of Ficus thonningii Blume. Two new flavonoids, thonningiol (1) and thonningiisoflavone (2) along with nineteen known compounds were isolated. β-Isoluteone (13) was isolated here for the first time from a natural source. Their structures were elucidated on the basis of spectroscopic evidence. Interestingly, thonningiisoflavone (2) and hydroxyalpinumisoflavone (21) showed strong DPPH radical scavenging activity with IC50=65.50μM and 68.20μM respectively compared to the standard BHA with IC50=44.20μM. The methanolic extract of figs, taxifolin (14), conrauiflavonol (17) and shuterin (19) exhibited moderate antimicrobial activity against six micro-organisms with MIC below 1.5mg/mL.

Investigation of total phenolic content and antioxidant activities of Azadirachta indica roots.

The present study was an attempt to study total phenolic content and antioxidant property of the crude ethanolic extract of the roots of Azadirachta indica (A. indica). To evaluate the antioxidant properties of the crude extract, some complementary test systems, namely DPPH free radical scavenging assay, reducing power assay, and ferrous ion chelating ability and determination of total phenolic content were conducted. In DPPH free radical scavenging test, IC50 value of the crude extract was found to be fairly significant (13.81±0.06 μg/ml) while compared with that of the reference standards, ascorbic acid and BHA (2.12±0.02 and 4.87±0.05 μg/ml, respectively). In reducing power assay, the maximum absorbance for the extract was found to be 1.523±0.026 at100 μg/ml compared with standard ascorbic acid and BHA (2.811±0.013 μg/ml and 2.031±0.019 μg/ml, respectively). The IC50 value of the extract as percentage of Fe(++) ion chelating ability was determined as 19.01±0.024 μg/ml where EDTA showed 8.87±0.035 μg/ml. The total phenolic amount was also calculated quite high in the extract (238.81±0.98 mg/g of gallic acid equivalent). The assays showed the presence of significant antioxidant properties of the crude sample, which would justify its traditional use. However, it would be very interesting to investigate the possible causes and their mechanisms responsible for the antioxidant property of the plant A. indica.

Evaluation of in vitro free radical scavenging potential of Streptomyces sp. AM-S1 culture filtrate

The present study was carried out to determine the free radical scavenging potential of culture filtrate of Streptomyces sp. AM-S1. Antioxidant activity of culture filtrate, lyophilized culture filtrate and ethyl acetate extract of Streptomyces sp. AM-S1 was determined by various in vitro assays such as ferric reducing power assay, phosphomolybdenum reduction, DPPH and ABTS radical scavenging activities. The results revealed that the culture filtrate of Streptomyces sp. AM-S1 effectively scavenged DPPH (IC50 90.2μl/ml) and ABTS (IC50 13.2μl/ml) radicals in a concentration dependent manner. In all the assays, ethyl acetate extract registered higher antioxidant activity when compared with the lyophilized culture filtrate (LCF). In addition, ethyl acetate extract (1123.4μmole Fe(II)/mg extract) exhibited higher ferric reducing activity than the standard BHA (814.4μmole Fe(II)/mg extract). Further works are needed on the isolation and identification of antioxidant molecules from the ethyl acetate extract of Streptomyces sp. AM-S1 culture filtrate.

Physico-chemical characteristics of wild plum fruits (Prunus spinosa L.)

The genotypic variation on physico-chemical characteristics of wild grown plums (Prunus spinosa L.) was investigated. The total phenolic contents were in a range of 117 to 407 mg GAE/100 g FW. The vitamin C was highest in yellow colored fruits (12.10 mg/100 ml). The total soluble solid content varied between 9.40% (yellow colored) and 12.90% (dark purple colored) fruits. Antioxidant activity of dark purple, red and yellow skin colored plum fruits were found between 71.15-78.99% which lowers than standard BHA (82.07%).

Evaluation of anti-inflammatory and antioxidant potential of the kernel root of Xylocarpus mekongensis (Lamk.) M. Roem

Anti-inflammatory and antioxidant activities of the crude ethanolic extract of the kernel root of Xylocarpus mekongenesis (Lamk.) M. Roem. (Meliaceae) was evaluated in different methods. The extract showed significant anti-inflammatory activity in carrageenan-induced oedema test in rats showing 69.42 % reduction in the paw volume (P < 0.01) comparable to that produced by the standard drug indomethacin (70.25 %) at 24 h when the extract was fed at the dose of 400 mg/kg body weight. The percentage inhibition of the oedema paw volume was statistically significant (p < 0.05) when compared with the indomethacin treated animals at 0.5, 1, 2, 4 and 6 h. The extract exhibited IC50 value 4.78 μg/ml in DPPH free radical scavenging activity test that was found to be very significant and comparable to that of the standard antioxidants ascorbic acid and BHA (1.9372 and 4.81 μg/ml) respectively. In nitric oxide radical scavenging test, the IC50 value was also found fairly significant 57.37 μg/ml when compared to standard ascorbic acid (36.81 μg/ml). In reducing power test, the maximum absorbance of the same extract was observed as 1.923 at 100 μg/ml and that of ascorbic acid and BHA were 2.8111 and 1.0314 respectively. The extract showed highest antioxidant capacity at 100 μg/ml concentration expressed as equivalents of ascorbic acid (1.44). The total phenolic content of the extract was also found quite high (342.15 ± 0.92 mg/g of gallic acid equivalent). Therefore, the results of this study suggest the probable anti-inflammatory and antioxidant activities of the ethanolic extract of X. mekongenesis kernel root and justify its use in traditional medicines.

In vitro free radical scavenging and DNA damage protective property of Coriandrum sativum L. leaves extract.

Coriandrum sativum L. (coriander), an everyday spice in the Indian kitchen is known to add flavor to the cuisine. It is an annual herb belonging to the Apiaceae (Umbellifera) family. The hydro-alcohol extract of Coriandrum sativum L. at the dose of 1mg/ml was subjected to a series of in vitro assays viz. 2, 2'- diphenyl-1-picrylhydrazyl, lipid peroxidation by thiobarbituric acid, reducing power and nitric oxide (NO) radical scavenging in order to study its antioxidant efficacy in detail. The amount of flavonoids in 70% ethanol extract was found to be 44.5μg and that of the total phenols was 133.74μg gallic acid equivalents per mg extract. The extracts of the leaves showed metal chelating power, with IC50 values, 368.12μg/ml where as that of standard EDTA was 26.7μg/ml. The IC50 values for 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid radical scavenging was 222μg/ml where as that of standard ascorbic acid was 22.6μg/ml. The NO scavenging activity of the extract of the leaves showed IC50 value of 815.6μg/ml; at the same time the standard BHA had 49.1μg/ml. All the plant extracts provided DNA damage protection; however, the protection provided at the dose of 8μg/ml was comparable to that of standard gallic acid. The Coriandrum sativum leaf extract was able to prevent in vitro lipid peroxidation with IC50 values; 589.6μg/ml where as that of standard BHA was 16.3μg/ml. Our results also showed significant ferric reducing power indicating the hydrogen donating ability of the extract. This study indicated the potential of the leaf extract as a source of natural antioxidants or nutraceuticals that could be of use in food industry with potential application to reduce oxidative stress in living system.

Evaluation of in-vitro Antioxidant Properties of Hydroalcoholic Solution Extracts Urtica dioica L., Malva neglecta Wallr. and Their Mixture.

The study was aimed at evaluating the antioxidant activity of hydroalcoholic solution extracts of Urtica dioica L. (UD), Malva neglecta Wallr. (MN) plants and their mixture. In this study, flower (UDF), root (UDR), seed (UDS) and leaf (UDL) parts of UD and flower (MNF) and leaf (MNL) parts of MN were used. The antioxidant properties of hydroalcoholic extracts and their mixture were evaluated using different antioxidant tests such as total antioxidant activity, reducing power, superoxide anion radical scavenging, hydrogen peroxide scavenging, free radical scavenging, and metal chelating activity for comparison. In addition, total phenolic compounds in the extracts of both plants were determined as catechin equivalent. The various antioxidant activities were compared to natural and synthetic standard antioxidants such as BHA, BHT and α-tocopherol. According to FTC method, the both extracts exhibited strong total antioxidant activity. At the concentration of 100 μg/mL, Hydroalcoholic extracts of UDS, UDR, UDF, UDL, MNF, MNL, and UD-MN showed 81.7%, 79.8%, 78.3%, 76.4%, 77.3%, 74.1%, and 80.7%, respectively. Comparable, 100 μg/mL of standard antioxidants BHA, BHT and α-tocopherol exhibited 66.2%, 70.6%, and 50.1% inhibition on peroxidation of linoleic acid emulsion, respectively. In addition, UD-MN showed strong superoxide anion radical scavenging activity comparable with UDR, UDF, UDL, MNF, and MNL. Based on the findings, plants mixture was commonly found to have synergistically higher antioxidant activity.

Synthesis, anti-oxidant activity, and cytotoxicity of salicyloyl derivatives of estra-1,3,5(10)-triene and androst-5-ene

AbstractSince many estrane and androstane derivatives exhibit cytotoxic, anti-oxidant, or anti-hormone activity, new steroidal derivatives were synthesised from appropriate estrogen or androgen precursors in order to obtain potential therapeutics for the treatment of steroid-dependent diseases. Starting from estradiol (I), 6-oxo derivatives V and VII were prepared. 17β-Salicyloyl-6-oxo derivatives VI and VIII were synthesised by the reaction of compounds V or VII with methyl salicylate in the presence of sodium. 17β-Salicyloyloxy estradiol IX was prepared from estradiol. Beckmann fragmentation of 16-oxyimino alcohols XII and XIII with methyl salicylate yielded corresponding D-seco derivatives XIV and XV. Simultaneous fragmentation and acylation of compound XII resulted in 3β-salicyloyl-D-seco derivative XVI which was also obtained from compound XIV. Anti-oxidant assays of the newly synthesised compounds V-IX, XIV, and XVI indicated a stronger capacity for hydroxyl radical scavenging, and a weaker capacity for DPPH radical scavenging, compared with the standard anti-oxidants BHA and BHT. Compounds V, XIV, and XVI showed higher or the same activity as BHT. The cytotoxicity of new compounds was evaluated against human breast and prostate carcinoma cells. Compound VI exhibited strong cytotoxicity against MDA-MB-231 cells; compound XIV exhibited strong cytotoxicity against PC-3 cell line, while compound VII moderately inhibited the growth of PC-3 cells.

Mushrooms as possible antioxidant and antimicrobial agents.

The aim of the study is to examine in-vitro antioxidant and antimicrobial activity of the acetonic and methanolic extracts of the mushrooms Boletus aestivalis, Boletus edulis and Leccinum carpini. Antioxidant activity was evaluated by using free radical scavenging activity and reducing power. In addition, total content of phenol and flavonoid in extracts were determined as pyrocatechol equivalent, and as rutin equivalent, respectively. As a result of the study acetonic extracts from Boletus edulis was more powerful antioxidant activity with IC50 value of 4.72 μg/mL which was similar or greater than the standard antioxidants, ascorbic acid (IC50 = 4.22 μg/mL), BHA (IC50 = 6.42 μg/mL) and α-tocopherol (IC50 = 62.43 μg/mL). Moreover, the tested extracts had effective reducing power. A significant relationship between total phenolic and flavonoid contents and their antioxidative activities was significantly observed. The antimicrobial activity of each extract was estimated by determination of the minimum inhibitory concentration by using microdilution plate method against five species of bacteria and five species of fungi. Generally, the tested mushroom extracts had relatively strong antimicrobial activity against the tested microorganisms. The minimum inhibitory concentration for both extracts related to the tested bacteria and fungi were 1.25 - 10 mg/ mL. The present study shows that tested mushroom species demonstrated a strong antioxidant and antimicrobial activity. It suggests that mushroom may be used as good sources of natural antioxidants and for pharmaceutical purposes in treating of various deseases.

Extraction, characterization and in vitro antioxidative potential of chitosan and sulfated chitosan from Cuttlebone of Sepia aculeata Orbigny, 1848

ObjectiveTo investigate antioxidant potency of chitosan and sulfated chitosan in various established in vitro systems, such as superoxide (O2-)/hydroxyl (-OH) radicals scavenging, reducing power, metal ion chelating. MethodsChitosan was prepared from deacetylation of chitin from cuttlefish. FTIR, degree of acetylation and mineral studies was carried out from the chitosan. Chitosan was converted into sulfated chitosan using DMF/HClSO3 and their antioxidant activity was tested. ResultsMineral content of chitosan was Ca – 30 ppm, Na- 0.092 ppm, Cu-0.172 ppm, Mg- 3.601, Mn- 0.264 and Zn- 0.924 ppm, DA was 49.9% (IR spectroscopy) and 40.56% (UV spectrophotometer) recorded. Cuttlefish chitosan showed high level of superoxide radical scavenging activity (88.6%) and hydroxyl radical scavenging activity (72.1%), moderate activity was noted in chelating activity (62.6% at 100 μgm/mL). At the same time low level of reducing power was observed (0.300 Absorbance at 0.75 mg/mL) when compared to standard BHA and Ascorbic acid (2.305 and 2.05 Absorbance). ConclusionsFinally, the scavenging rate, reducing power, chelating and reducing power of sulfated chitosan increased with their increasing concentration.

Antioxidant, Anti-Glycation and Anti-Inflammatory Activities of Phenolic Constituents from Cordia sinensis

Nine compounds have been isolated from the ethyl acetate soluble fraction of C. sinensis, namely protocatechuic acid (1), trans-caffeic acid (2), methyl rosmarinate (3), rosmarinic acid (4), kaempferide-3-O-β-D-glucopyranoside (5), kaempferol-3-O-β-D-glucopyranoside (6), quercetin-3-O-β-D-glucopyranoside (7), kaempferide-3-O-α-L-rhamnopyranosyl (1→6)-β-D-glucopyranoside (8) and kaempferol-3-O-α-L-rhamno-pyranosyl (1→6)-β-D-glucopyranoside (9), all reported for the first time from this species. The structures of these compounds were deduced on the basis of spectroscopic studies, including 1D and 2D NMR techniques. Compounds 1–9 were investigated for biological activity and showed significant anti-inflammatory activity in the carrageen induced rat paw edema test. The antioxidant activities of isolated compounds 1–9 were evaluated by the DPPH radical scavenging test, and compounds 1, 2, 4 and 7–9 exhibited marked scavenging activity compared to the standard BHA. These compounds were further studied for their anti-glycation properties and some compounds showed significant anti-glycation inhibitory activity. The purity of compounds 2–5, 8 and 9 was confirmed by HPLC. The implications of these results for the chemotaxonomic studies of the genus Cordia have also been discussed.