Year-end Sale % OFF 
Abstract
Different biological activities of Hypericum perforatum L. dry extract was tested in this study. Antioxidant activity was evaluated by four different assays. Antineurodegenerative activity was tested using acetylcholinesterase (AChE) and tyrosinase inhibitory activity assays. In all four antioxidant assays, concentration dependent manner was noticed in the activity of tested extract. The most active was the highest applied concentration (500 μg/ml). In DPPH test, extract applied in dose of 200 μg/ml showed similar or higher radical scavenging activity compared to the dose of 100 μg/ml applied for standard substances BHA and BHT. The lowest antioxidant activity for the extract was detected in ABTS test. All applied concentrations of dry extract were significantly more potent than vitamin C in s -carotene test. Moreover, the extract reached 40.31 and 45.56 % of the inhibition in AChE and TYR assays, respectively. In both cases, the most active concentration of the extract was 200 µg/ml.
Highlights
Hypericaceae includes 8 genera, with about 360 species, but only Hypericum perforatum
In DPPH test, extract applied in concentration of 200 μg/ml showed similar or higher radical scavenging activity compared to the concentration of 100 μg/ml applied for standard substances BHA and BHT
The lowest antioxidant activity for the extract was detected in ABTS test
Summary
Hypericaceae includes 8 genera, with about 360 species, but only Hypericum perforatum John’s wort have been commonly used in a form of tea, hydroalcoholic extracts or oil extracts, while standardized, dry hydroalcoholic extracts are used for the preparation of herbal products, i.e. capsule Such solid preparations are recommended in the treatment of mild to moderate depressive episode (Butterweck and Schmidt, 2007). Chemical composition of H. perforatum is very complex and the several groups of compounds are considered as the active principles, i.e. naphthodianthrone derivatives hypericin and pseudohypericin, phloroglucinol derivatives hyperforin and adhyperforin, flavonoids and xanthones (Del Monte et al, 2015; Zdunicet al., 2017; WHO, 2002) Those compounds are connected with variety of biological activities such as antibacterial, antioxidant, antiviral, antiinlammatory (Šavikin et al, 2007; Zdunicet al., 2009; Saddiqe et al, 2010; Zdunicet al., 2017). It is considered as neuroprotective agent that can prevent neurodegenerative pathologies by regulating neurotransmitter release in animal model, as well as by its antioxidant and antiinflammatory activity (Grundmann et al, 2010; Jungke et al, 2011; Klusa et al, 2001)
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
