Stages Of Evolution Research Articles

Suppression of dystroglycan function accompanies pancreatic acinar-to-ductal metaplasia and favours dysplasia development.

The basement membrane (BM) is among the predominant microenvironmental factors of normal epithelia and of precancerous epithelial lesions. Evidence suggests that the BM functions not only as a barrier to tumour invasion but also as an active tumour-suppressing signalling substrate during premalignancy. However, the molecular foundations of such mechanisms have not been elucidated. Here we explore potential tumour-suppressing functions of the BM during precancer evolution, focusing on the expression and function of the extracellular matrix receptor dystroglycan in the pancreas and pancreatic disease. We show that the dystroglycan protein is highly expressed in the acinar compartment of the normal pancreas but lower in the ductal compartment. Moreover, there is a strong suppression of dystroglycan protein expression with acinar-to-ductal metaplasia in chronic pancreatitis and in all stages of pancreatic precancer and cancer evolution, from acinar-to-ductal metaplasia to dysplasia to adenocarcinoma. The conditional knockout of dystroglycan in the murine pancreas produced little evidence of developmental or functional deficiency. However, conditional deletion of dystroglycan expression in the context of oncogenic Kras expression led to a clear acceleration of pancreatic disease evolution, including accelerated dysplasia development and decreased survival. These data establish dystroglycan as a suppressor of pancreatic dysplasia development and one that is muted in chronic pancreatitis and at the earliest stages of oncogene-induced transformation. We conclude that dystroglycan is an important mediator of the tumour-suppressing functions of the BM during precancer evolution and that reduced dystroglycan function increases cancer risk, highlighting the dynamics of cell-BM interactions as important determinants of early cancer progression. © 2024 The Pathological Society of Great Britain and Ireland.

Structural evolution of the Handun salt diapir, Zagros fold and thrust belt, southern Iran

The Fars region, in the Zagros fold and thrust belt, hosts a wide range of diapirs piercing over 10 km of stratigraphic sequence. Comprising Precambrian to Early-Cambrian Hormuz Salt, these diapirs exhibit a prolonged history of evolution. Outcrop evidence for understanding the diapir deformation history is mostly limited to the Cenozoic contractive phase, and the seismic data lacks the necessary quality for an exhaustive understanding of the deepest structure's geometries. Through regional and field evidence we unravel the Handun salt structure evolution and propose a sequential restoration to describe the key deformational events. Our study presents a field-based novel regional balanced cross-section and a 3D-geological model, and addresses the role of structural inheritances and the position of the Handun diapir with respect to the decupled basement. The performed field studies describe folds and unconformities related to Cenozoic halokinetic sequences with exceptional clarity. It was possible to observe changes of the diapir activity along the structure and provide field evidence for the relative timing and kinematics of primary and secondary welding. Finally, our data suggest that the Handun diapir formed in the early Paleozoic above the shoulder of a basement extensional fault, and was partially translated above its southern hanging-wall during the shortening. In the Paleocene a sustained ratio of salt rise rate was enhanced by the Zagros/Oman contraction. In response to the Oligocene continental collision, the diapir was profusely supplied with salt, which flared upward to form overhangs. Since the middle Miocene the salt supply slowly depleted, with the diapiric walls remaining near the surface but tapering upward, probably due to primary welding or increased sedimentation. Secondary welding occurred post-Pliocene in the last stages of the diapir evolution with consequent development of a secondary minibasin.

Element-Specific Ultrafast Lattice Dynamics in Monolayer WSe2.

We study monolayer WSe2 using ultrafast electron diffraction. We introduce an approach to quantitatively extract atomic-site-specific information, providing an element-specific view of incoherent atomic vibrations following femtosecond excitation. Via differences between W and Se vibrations, we identify stages in the nonthermal evolution of the phonon population. Combined with a calculated phonon dispersion, this element specificity enables us to identify a long-lasting overpopulation of specific optical phonons and to interpret the stages as energy transfer processes between specific phonon groups. These results demonstrate the appeal of resolving element-specific vibrational information in the ultrafast time domain.

Studying binary systems in Omega Centauri with MUSE – I. Detection of spectroscopic binaries

ABSTRACT NGC 5139 ($\omega$ Cen) is the closest candidate of a nuclear star cluster that has been stripped of its host galaxy in the Milky Way. Despite extensive studies through the last decades, many open questions about the cluster remain, including the properties of the binary population. In this study, we use MUSE multi-epoch spectroscopy to identify binary systems in $\omega$ Cen. The observations span 8 yr, with a total of 312 248 radial velocity measurements for 37 225 stars. Following the removal of known photometric variables, we identify 275 stars that show RV variations, corresponding to a discovery fraction of $1.4\pm 0.1~{{\ \rm per\ cent}}$. Using dedicated simulations, we find that our data are sensitive to $70 \pm 10~{{\ \rm per\ cent}}$ of the binaries expected in the sample, resulting in a completeness-corrected binary fraction of $2.1\pm 0.4~{{\ \rm per\ cent}}$ in the central region of $\omega$ Cen. We find similar binary fractions for all stellar evolutionary stages covered by our data, the only notable exception being the blue straggler stars, which show an enhanced binary fraction. We also find no distinct correlation with distance from the cluster centre, indicating a limited amount of mass segregation within the half-light radius of $\omega$ Cen.

Mechanisms of Reservoir Space Preservation in Ultra-Deep Shales: Insights from the Ordovician–Silurian Wufeng–Longmaxi Formation, Eastern Sichuan Basin

This study aims to explore the reservoir characteristics and formation mechanisms of ultra-deep shale gas in the Ordovician–Silurian Wufeng–Longmaxi Formation in the Sichuan Basin in order to provide theoretical support and practical guidance for the exploration and development of ultra-deep shale gas. With recent breakthroughs in ultra-deep shale gas exploration, understanding its organic matter development, mineral composition, and reservoir space characteristics has become particularly important. The background of this research lies in the significant potential of ultra-deep shale gas, which remains inadequately understood, necessitating an in-depth analysis of its pore structure and reservoir quality. Through a systematic study of the ultra-deep shale in well FS1 of Sichuan Basin, that the following was found: (i) The ultra-deep shale in the Wufeng–Longmaxi Formation is mainly composed of quartz and clay minerals, exhibiting high total organic carbon (TOC) and high porosity characteristics, indicating it is in an overmature thermal evolution stage. (ii) Organic pores and microcracks in the ultra-deep shale are more developed compared to middle-shallow and deep shale, forming a complex pore structure that is conducive to gas storage. (iii) In the diagenesis process, the dissolution and recrystallization of the biogenic skeleton promote the cementation between autogenetic quartz particles, forming a rigid skeleton that effectively inhibits the impact of mechanical compaction. (iv) The overpressure environment created by the hydrocarbon generation process, along with gas production from hydrocarbon cracking, can effectively offset the mechanical compaction of overburden pressure on micropores, and this overpressure environment also promotes the further development of microfractures, which is beneficial for the development and preservation of ultra-deep shale pores. In summary, this study not only reveals the reservoir characteristics and formation mechanisms of ultra-deep shale but also provides essential references for the exploration and development of ultra-deep shale gas in the Sichuan Basin and similar regions, emphasizing the ongoing significance of research in this field.

Relationship between root system-soil C:N:P and soil microbial diversity at different evolutionary stages of Caragana tibetica scrub in arid desert grassland, Northern China.

Scrub root systems play a crucial role in preventing soil erosion and nutrient loss. However, the effects of the root system configuration on soil nutrient dynamics and microbial changes at various evolutionary stages remain poorly understood. In this study, we investigated the relationship between soil physical and chemical properties and the diversity of bacteria and fungi throughout the evolutionary stages of the scrub root systems. This was achieved through a combination of whole-root excavation and root tracing techniques. The results indicated that root diameter was the main factor contributing to the continuous increase in carbon (C): phosphorus (P) and nitrogen (N): P ratios as the scrub sand pile developed. The soil organic carbon (SOC), total soil nitrogen (TN), and total soil phosphorus (TP) contents in the soil in the four evolutionary stages were the highest during the developmental stage, but the change in TP content was not statistically significant (P > 0.05). Partial least squares path modeling and redundancy analysis (RDA) indicated that root system stoichiometric C and N contents were positively correlated with microbial diversity (R2 = 0.85). There was no correlation between the evolutionary stage and soil nutrient TN, whereas soil nutrient TN was negatively correlated with microbial diversity (R2 = -0.92). These findings elucidate the relationship between the evolutionary stage of root chemical measurement characteristics, soil elements and microorganisms, and their subsequent effects on root elemental composition and microbial diversity. This study enhances the current understanding of plant-soil interactions in desert steppe ecosystems.

Diagenesis of marine-continental transitional shale from the Upper Permian Longtan Formation in southern Sichuan Basin, China

Abstract Upper Permian Longtan Formation transitional shale has become an important exploration layer, but the Longtan Formation shale (LFS) has a complex mineralogical composition, which affects the subsequent diagenesis and diagenetic evolution, and restricts the subsequent geologic exploration of shale gas. In this article, the observation of drilling cores, argon ion polishing-scanning electron microscope, Vitrinite reflectance, and X-ray diffractometer were used to analyze the type and characteristics of diagenesis of the LFS and clarify the stage of diagenetic evolution. The results show that the main diagenesis in the LFS is compaction, cementation, thermal maturation of organic matter (OM), dissolution, and transform of clay minerals. Among them, OM hydrocarbon generation, clay mineral transformation, and dissolution are pore-enhancing diagenetic events. Compaction and cementation are pore-reducing diagenetic events. The transitional and marine shales have similar characteristics of diagenesis, but there are big variations in the diagenesis of OM hydrocarbon, authigenic quartz, and siderite. The complex depositional environments of the marine-continental transition environment have resulted in a variety of rock types, which in turn influenced the diagenesis types and diagenetic evolution process. Compared with the transitional shales of the Shanxi and Taiyuan formations in the South China North Basin, the LFS are characterized by high clay content, low quartz content, complex mineral compositions, and a higher degree of thermal evolution.

A Study of Stochastic Low-frequency Variability for Galactic O-type Stars

In order to explore how the ubiquitous stochastic low-frequency (SLF) variability of O-type stars is related to various stellar characteristics, we compiled a sample of 150 O-type stars observed via ground-based spectroscopic surveys, alongside photometric data obtained from the Transiting Exoplanet Survey Satellite (TESS). We analyzed 298 light curves obtained from TESS Sectors 1–65 for the stars in our sample. Leveraging the spectroscopic parameters, we used Bonnsai to determine masses, radii, fractional main-sequence ages, and mass-loss rates for stars of our sample. Subsequently, we identified possible correlations between the fitted parameters of SLF variability and stellar properties. Our analysis unveiled four significant correlations between the amplitude and stellar parameters, including mass, radius, fractional main-sequence ages, and mass-loss rate. For stars with ≳30 M ⊙, we observed a decrease in characteristic frequency and steepness with increasing radius. Finally, we compared various physical processes that may account for the SLF variability with our results. The observed SLF variability may arise from the combined effects of the iron convection zone (FeCZ) and internal gravity waves (IGWs), with IGWs potentially more dominant in the early stages of stellar evolution, and the contribution of FeCZ becoming more significant as stars evolve. Meanwhile, our results indicate that the SLF variability of O-type stars bears certain signatures of the line-driven wind instability and granulation.

Direct estimates of nitrogen abundance for Seyfert 2 nuclei

Abstract We derive the nitrogen and oxygen abundances in the Narrow Line Regions (NLRs) of a sample of 38 local (z < 0.4) Seyfert 2 nuclei. For that, we consider narrow optical emission line intensities and direct estimates of the electron temperatures (Te-method). We obtain a new theoretical expression for the nitrogen Ionization Correction Factor [ICF($\rm N^{+}$)] for NLRs. Applying this new ICF, we unexpectedly find that NLRs and disk H ii regions exhibit similar ICF distributions. We find nitrogen abundances in the range $7.6 \: < \: \rm 12+log(N/H) \: < \: 8.6$ (mean value 8.06 ± 0.22) or $\rm 0.4 \: < \: (N/N_{\odot }) \: < 4.7$, in the metallicity regime $8.3 \: < \: \rm 12+log(O/H) \: < \: 9.0$. Our results indicate that the dispersion in N/H abundance for a fixed O/H value in AGNs of ∼0.2 dex agrees with that for disc H ii regions with similar metallicity. We show that Seyfert 2 nuclei follow a similar (N/O)-(O/H) relation to the one followed by star-forming objects. Finally, we find that active galaxies called as ”nitrogen-loud” observed at very high redshift (z > 5) show N/O values in consonance with those derived for local NLRs. This result indicates that the main star-formation event is completed in the early evolution stages of active galaxies.

Research on the Evolution and Core Structure of Low Mass Stars

This article studies the complete evolution process of low-quality stars from main sequence stars to red giants, and then to white dwarfs, and analyzes in detail the physical characteristics of the stellar core. By applying the stellar structure equation, energy generation rate formula, and degeneracy pressure formula, this paper derives the evolution laws of temperature and density of the stellar core over time, and explores the influence of these physical properties on different stages of stellar evolution. Through detailed analysis of the stellar core at various stages, this article reveals the physical evolution mechanism of low mass stars during their lifecycle.

Research on the recycling decision of WEEE in rural China under dual supervision

Against the backdrop of promoting green, low-carbon, and high-quality development, this paper aims to promote efficient recycling of waste electrical and electronic equipment (WEEE) in rural China. In this paper, considering the integrated development of urban and rural areas and the standardization of industrial recycling system, under the joint action of the extension of producer responsibility and “dual regulation” of supply and marketing cooperatives, the evolutionary game model and system dynamics model of three-level recycling network of farmers, supply and marketing cooperatives and retailers are established. The mechanism of the participants to promote the recycling of WEEE in rural areas is discussed and the strategic choices and interactive relationships of various entities in the evolutionary process were used to analyze through the evolutionary game method. Meanwhile, using the theory of system dynamics, the main influencing factors of different evolution stages and the dynamic change process of the system are analyzed. The results show that: (1) supply and marketing cooperatives, retailers, and farmers can initially tend to participate in and supervise the recycling of WEEE; however, (2) they can finally achieve strong supervision, actively undertake and participate in the recycling and stabilization stable strategy of rural WEEE depends on their benefits and cost of expenditure expenditures are reasonable. (3) The strategic choice of supply and marketing cooperatives has the most significant impact on the strategic choice of retailers and the strategic choice of retailers has the most significant impact on the strategic choice of farmers.

Dynamical Evolution of Four Old Galactic Open Clusters Traced by Their Constituent Stars with Gaia DR3

We investigate the evolutionary stages of four open clusters—Berkeley 39, Collinder 261, NGC 6819, and NGC 7789—of ages ranging from 1.6 to 6 Gyr. These clusters have previously been classified into dynamically young and intermediate age groups based on the segregation level of BSS with respect to red-giant-branch stars and main-sequence stars, respectively. We identify members of these four clusters using the ML-MOC algorithm on Gaia DR3 data. To examine the relative segregation of cluster members of different evolutionary stages, we utilize cumulative radial distributions, proper motion distributions, and spatial distributions in galactocentric coordinates. Our analysis shows that Berkeley 39 and NGC 6819 exhibit moderate signs of population-wise segregation from evolved to less-evolved members. NGC 7789 shows signs of mass segregation only in the cumulative radial distributions. On the other hand, Collinder 261 exhibits high segregation of BSS in the cumulative radial distribution, while other populations show the same level of segregation.

The Final Pliocene and Early Pleistocene Faunal Dispersals from East to Europe and Correlation of the Villafranchian Biochronology between Eastern and Western Europe

The Villafranchian stage in the mammal fauna evolution in Eurasia (ca. 3.6/3.4 Ma—ca. 1.2 Ma) is associated with the beginning of the formation of the modern appearance of the mammal megafauna of today’s Palaearctic. The cooling and the aridification starting with the beginning of the Early Pleistocene gradually eliminated the quasi-tropical appearance of the Late Neogene landscapes and fauna of Europe. The time from the Mid-Piacenzian (ca. 3.3–3.0 Ma) to the end of the Early Pleistocene was a time of particularly intense dispersal of species, of faunal exchange between Eurasia and Africa, and of the entry of new mammals into Europe from the East. That is why the correlation of the biochronology of the Villafranchian fauna between Eastern and Western Europe is of particular interest. Accumulated data make possible a more precise correlation of these faunas today. A correlation of selected Eastern European localities with established faunal units and MNQ zones is made in the present work. Usually, the dispersal from Asia or from E. Europe to W. Europe is instantaneous from a geological point of view, but in a number of cases, reaching W. Europe happens later, or some species known to be from Eastern Europe do not reach Western Europe. The main driving forces of the faunal dispersals, which are the key bioevents in the faunal formation, are climate changes, which in turn, affect the environment. We can summarize the following more significant Villafranchian bioevents in Europe: the End Pliocene (Early Villafranchian: MNQ16) turnover related to the first appearance of a number of taxa, for example, felids, canids, proboscideans, and ungulates; the Quaternary beginning turnover. Correlated with this are the beginning of the Middle Villafranchian, which should be placed at about 2.6 Ma; the Coste San Giacomo faunal unit turnover (Senèze and Slivnitsa localities should be included here, and the FU itself, at the very beginning of the late Villafranchian (=MNQ18a)); the Pachycrocuta event at the very beginning of the Olivola FU; and the events related to the Late Villafranchian/Epivillafranchian bounfary.

Paradigms of people management: Human resource strategies during an economic downturn

Since the Industrial Revolution, there has been an evolution in the paradigms under which the industrial worker is perceived and dealt with. These paradigms can be briefly listed in the order of their evolutionary stage as: the food-gatherer, the economic man, the social man, the resourceful man, and the enterprising man. Each of them is a combination of two basic paradigms in different proportions, namely, the outsider paradigm and the partnership paradigm. Obviously, the paradigmatic perspectives of management about their workers will have a significant influence on how they treat their workers, which may become especially conspicuous during recessions and other kinds of hard times. It was in this context that we designed a study to understand the human resource strategies of companies during a period of recession. Data for this study was collected through the content analysis of 46 published cases, wherein we developed the ratings of two sets of variables, namely: the external and internal environments of the company and the strategic actions taken by the respective managements. A surprising finding of the study is that the correlations between the environmental factors and the strategy factors were small and non-significant; moreover, the correlations involving the external environment were smaller than those involving the internal environment. Hence, it may be inferred that strategic actions are influenced primarily by the paradigmatic perspectives of management rather than environmental factors. In order to identify the different types of paradigmatic perspectives, we have further carried out a cluster analysis to develop a taxonomy of paradigms. The results showed that there are five sub-paradigms, which are: (1) Pacifiers, constituting 35% of the sample; (2) Modifiers, constituting 22%; (3) Molders, constituting 17%; (4) Enhancers, constituting 15%; and (5) Exploiters, constituting 11%. The limitations of the study and the implications of the findings are discussed in the concluding part.

The XRISM first-light observation: Velocity structure and thermal properties of the supernova remnant N 132D

Abstract We present an initial analysis of the X-Ray Imaging and Spectroscopy Mission (XRISM) first-light observation of the supernova remnant (SNR) N 132D in the Large Magellanic Cloud. The Resolve microcalorimeter has obtained the first high-resolution spectrum in the 1.6–10 keV band, which contains K-shell emission lines of Si, S, Ar, Ca, and Fe. We find that the Si and S lines are relatively narrow, with a broadening represented by a Gaussian-like velocity dispersion of $\sigma _v \sim 450$ km s$^{-1}$. However, the Fe He$\alpha$ lines are substantially broadened with $\sigma _v \sim 1670$ km s$^{-1}$. This broadening can be explained by a combination of the thermal Doppler effect due to the high ion temperature and the kinematic Doppler effect due to the SNR expansion. Assuming that the Fe He$\alpha$ emission originates predominantly from the supernova ejecta, we estimate the reverse shock velocity at the time when the bulk of the Fe ejecta were shock heated to be $-1000 \lesssim V_{\rm rs}$ (km s$^{-1}$) $\lesssim 3300$ (in the observer frame). We also find that Fe Ly$\alpha$ emission is redshifted with a bulk velocity of $\sim 890$ km s$^{-1}$, substantially larger than the radial velocity of the local interstellar medium surrounding N 132D. These results demonstrate that high-resolution X-ray spectroscopy is capable of providing constraints on the evolutionary stage, geometry, and velocity distribution of SNRs.

Synergistic evolution: The dynamic adaptation of SARS-CoV-2 and human protective immunity in the real world

SARS-CoV-2 is continually evolving with new variants to evade protective immunity and cause new infections. This study aimed to assess infection-acquired immunity and hybrid immunity against re-infection or severe COVID-19. During 2020-2023, we collected 890 serum samples from individuals infected with SARS-CoV-2 variants including wild type, D614G, Alpha, Delta, BA.1, BA.2, BA.2.76, BA.5.2, BF.7, XBB, and EG.5. The levels of serum neutralizing antibodies (NAbs) against 18 diverse SARS-CoV-2 variants were determined using a bead-based high-throughput broad neutralizing-antibody assay. In the initial wave of the COVID-19 pandemic, >75% of the patients demonstrated robust NAb responses against the ancestral SARS-CoV-2, during a period when vaccines were not yet available. After the emergence of the Omicron variant, the seroprevalence of anti-Omicron NAbs among the patients increased rapidly. By April 2023, when XBB variant was predominant, approximately 80% of the patients demonstrated >50% neutralization against the highly immune-evasive XBB lineages. Three serotypes of SARS-CoV-2, namely non-Omicron, Omicron, and XBB serotypes, were identified, with the strong likelihood of further changes occurring as the virus mutating. Generally, NAbs elicited by a previous serotype could not typically effectively protect against another serotype that emerges later in the evolutionary stages. Our results firstly demonstrated the synergistic evolution between host immunity and SARS-CoV-2 variants in the real world, which would be helpful to develop future vaccines and public health strategies.

Eager or passive? Decoding potential consumer profiles based on knowledge and perceptions regarding organic food products

Organic food-choice behaviour has gained paramount interest from researchers and marketers due to the growing inclination of consumers towards healthy and sustainable lifestyles. With regard to India, despite being a leading producer and exporter of organic products, its domestic market is still in nascent stages of evolution. To overcome the underlying attitude-intention-behaviour gap in such emerging markets, it is imperative to identify targetable segments based on factors that are relevant to these markets. The present study makes a novel attempt at segmenting potential organic consumers based on their knowledge and perceptions regarding organic products. Robust analytical procedures, including a two-stage cluster analysis and multiple discriminant analysis, were employed to draw consumer profiles based on a sample of 608 Indian consumers. The analysis resulted in four mutually exclusive segments, which exhibited significant heterogeneity with respect to various clustering and profiling variables, namely actual and perceived knowledge regarding organic products, price and availability perceptions, trust in organic products, and purchase intention. The aware enthusiasts displayed high knowledge, while both the aware and unaware enthusiasts exhibited favourable organic perceptions and high purchase intention and behaviour. The sceptics had positive perceptions but displayed a lack of trust in organic sellers and regulatory frameworks. The laggards exhibited the least favourable perceptions and had the lowest intention and purchase behaviour. The study identifies potential target segments of organic consumers and throws light on how consumers construe organic food products. Furthermore, marketing strategies are recommended to tap these segments, considering the core characteristics specific to each segment.

Explanation of the phenomenon of agroindustrial cooperation: general theoretical and sectoral contexts

The theoretical and methodological apparatus of scientific approaches (economic, interdisciplinary) is analyzed in terms of revealing the general theoretical context of the phenomenon of cooperation (preconditions, mechanics of manifestation, effects). Conclusions are drawn about the development of scientific understanding of its functions and role. The prototypes of the models of cooperative relations are established, the chronological order of change is determined, their – content is revealed. The evolutionary stages of development of the theory of agrarian cooperation are substantiated. The peculiarities of modification of methodological models of analysis of cooperation in modern conditions of scientific search are determined, the synthesized description of the studied category is given.

Effects of ovarian stimulation on embryo euploidy: an analysis of 12874 oocytes and 3106 blastocysts in cycles with preimplantation genetic testing for monogenic disorders.

Does ovarian stimulation and the ovarian response affect embryo euploidy? Ovarian stimulation and the ovarian response in women undergoing preimplantation genetic testing for monogenic disorders (PGT-M) cycles did not affect the rates of blastocyst euploidy. Whether or not ovarian stimulation in IVF-embryo transfer has potential effects on embryo euploidy is controversial among studies for several reasons: (i) heterogeneity of the study populations, (ii) biopsies being performed at different stages of embryo development and (iii) evolution of the platforms utilized for ploidy assessment. Patients who undergo PGT-M cycles typically have no additional risks of aneuploidy, providing an ideal study population for exploring this issue. A retrospective cohort study including embryos undergoing PGT-M was conducted at a single academically affiliated fertility clinic between June 2014 and July 2021. A total of 617 women with 867 PGT-M cycles involving 12874 retrieved oocytes and 3106 trophectoderm biopsies of blastocysts were included. The primary outcome of the study was median euploidy rate, which was calculated by dividing the number of euploid blastocysts by the total number of biopsied blastocysts for each cycle. Secondary outcomes included the median normal fertilization rate (two-pronuclear (2PN) embryos/metaphase II oocytes) and median blastulation rate (blastocyst numbers/2PN embryos). Comparable euploidy rates and fertilization rates were observed across all age groups, regardless of variations in ovarian stimulation protocols, gonadotropin dosages (both the starting and total dosages), stimulation durations, the inclusion of human menopausal gonadotrophin supplementation, or the number of oocytes retrieved (all P > 0.05). Blastulation rates declined with increasing starting doses of gonadotropins in women aged 31-34 years old (P = 0.005) but increased with increasing gonadotrophin starting doses in women aged 35-37 years old (P = 0.017). In women aged 31-34, 35-37, and 38-40 years old, blastulation rates were significantly reduced with increases in the number of oocytes retrieved (P = 0.001, <0.001, and 0.012, respectively). Limitations include the study's retrospective nature and the relatively small number of patients of advanced age, especially patients older than 40 years old, leading to quite low statistical power. Second, as we considered euploidy rates as outcome measures, we did not analyze the effects of ovarian stimulation on uniform aneuploidy and mosaicism, respectively. Finally, we did not consider the effects of paternal characteristics on embryo euploidy status due to the fact that blastocyst aneuploidy primarily originates from maternal meiosis. However, sperm factors might have an effect on embryo development and the blastulation rate, and therefore also the number of blastocysts analyzed. The exclusion of patients with severe teratozoospermia and the fact that only ICSI was used as the insemination technique for women undergoing PGT-M contributed to minimize the effect of paternal factors. Ovarian stimulation and response to stimulation did not affect blastocyst euploidy rates in women undergoing PGT-M cycles. However, in women aged 31-40 years old, there was a significant decline in blastulation rates as the number of retrieved oocytes increased. This study was supported by the National Natural Science Foundation of China (Grant No. 81701407, 82301826); the National Key Research and Development Program of China (2022YFC2702901, 2022YFC2703004); China Postdoctoral Science Foundation (2022M710261), and China Postdoctoral Innovation Talent Support Program (BX20220020). There is no conflict of interest. N/A.

Semantics of Transition: Death and Resurrection of the Hero in Film Fantasy at the Turn of the 20th—21st Centuries

The article is devoted to the study of human metaphysical experience through cinema art. The material of the study is widely known fantasy films (“The Lord of the Rings”, “The Matrix”, “Harry Potter”, “The Chronicles of Narnia”, “Avatar”), in which the sacral space is realized as a magical fairy tale or a dream, and the hero acting in it is identified with the concept of “soul” or “consciousness”. The aim of the study is to semantically and cognitively analyze the visual image of transition through death, which is offered to the viewer by cinematography, and to model the director’s concepts included in it. The author’s concept of transition is based on the works of M. Eliade, A. van Gennep, C.G. Jung, M.P. Hall and is revealed as a multi-phase process of soul transformation in the conditions of spiritual or perceptual crisis. It is established that the transition scenario in the films considered includes an “entry point” into another world, a series of initiations and an “exit point”. In this context, death and resurrection are the climactic phase and have different symbolic connotations, their essence depending on the prototype that the hero imitates. As it has been revealed, the imitation of cyclical forces of nature (solar, agrarian deities) by contemporary directors is interpreted by them as a long stage of soul maturation, which is no longer opposed to, but naturally precedes the imitation of the “divine archetype” (in the Christian tradition, Christ the Saviour). The axis of transition from forced submission to the endless cycle of renewal to the evolutionary stage of spiritual maturity is the act of “awakening” expressed in the willingness to voluntarily sacrifice oneself out of love and compassion for others. Death and resurrection in this case manifest the metaphysical transformation of the soul aspect into the spiritual one. At the same time, in the process of studying the film material, a tendency of a transgressive nature was detected, namely the replacement of the concept “soul” by the concept “brain”. As a result of identifying the functions of the human brain with the functions of a computer in the mental space, there is a danger of substitution of the divine image by artificial intelligence, which can provoke the blocking of the channel “soul – spirit” and the involutionary turn from spirit to matter.