Stable Fluorescence Research Articles

Probing Activation and Conformational Dynamics of the Vesicle-Reconstituted β2 Adrenergic Receptor at the Single-Molecule Level.

G-protein-coupled receptors (GPCRs) are structurally flexible membrane proteins that mediate a host of physiological responses to extracellular ligands like hormones and neurotransmitters. Fine features of their dynamic structural behavior are hypothesized to encode the functional plasticity seen in GPCR activity, where ligands with different efficacies can direct the same receptor toward different signaling phenotypes. Although the number of GPCR crystal structures is increasing, the receptors are characterized by complex and poorly understood conformational landscapes. Therefore, we employed a fluorescence microscopy assay to monitor conformational dynamics of single β2 adrenergic receptors (β2ARs). To increase the biological relevance of our findings, we decided not to reconstitute the receptor in detergent micelles but rather lipid membranes as proteoliposomes. The conformational dynamics were monitored by changes in the intensity of an environmentally sensitive boron-dipyrromethene (BODIPY 493/503) fluorophore conjugated to an endogenous cysteine (located at the cytoplasmic end of the sixth transmembrane helix of the receptor). Using total internal reflection fluorescence microscopy (TIRFM) and a single small unilamellar liposome assay that we previously developed, we followed the real-time dynamic properties of hundreds of single β2ARs reconstituted in a native-like environment─lipid membranes. Our results showed that β2AR-BODIPY fluctuates between several states of different intensity on a time scale of seconds, compared to BODIPY-lipid conjugates that show almost entirely stable fluorescence emission in the absence and presence of the full agonist BI-167107. Agonist stimulation changes the β2AR dynamics, increasing the population of states with higher intensities and prolonging their durations, consistent with bulk experiments. The transition density plot demonstrates that β2AR-BODIPY, in the absence of the full agonist, interconverts between states of low and moderate intensity, while the full agonist renders transitions between moderate and high-intensity states more probable. This redistribution is consistent with a mechanism of conformational selection and is a promising first step toward characterizing the conformational dynamics of GPCRs embedded in a lipid bilayer.

Luminescent terphen[3]arene sulfate-activated FRET assemblies for cell imaging

Multicharged supramolecular assemblies based on luminescent macrocycle play an important role in extending their optical properties and functions. Herein, we reported macrocyclic supramolecular assemblies based on luminescent terphen[3]arene sulfate (TP[3]AS) and tetraphenylethylene pyridinium (TPE-4Py) through electrostatic interactions, host-guest encapsulation and π-π stacking interactions. Förster resonance energy transfer (FRET) process from TP[3]AS to TPE-4Py was achieved with the energy transfer efficiency of 99.9%, accompanied by TPE-4Py fluorescence emission bathochromic shifted of 15 nm and enhanced by 1.68 times in PBS solution. In contrast, other non-luminescent sulfato-β-cyclodextrin and sulfobutylether-β-cyclodextrin only can enhance the fluorescence intensity of TPE-4Py without bathochromic shift. Due to the strong fluorescence and good stability of TPE-4Py@TP[3]AS, it can be used for optical imaging in living cells, which provided an effective approach for the construction of assembling-confined luminescent biomaterials.

Label-free single-cell analysis in microdroplets using a light-scattering-based optofluidic chip

Droplet-based single-cell analysis is a very powerful tool for studying phenotypic and genomic heterogeneity at single-cell resolution for a variety of biological problems. In conventional two-phase droplet microfluidics, due to the mismatch in optical properties between oil and aqueous phases, light scattering mainly happens at the oil/water interface that disables light-scattering-based cell analysis confined in microdroplets. Detection and analysis of cells in microdroplets thus mostly rely on the fluorescence labeling of cell samples, which may suffer from complex operation, cytotoxicity, and low fluorescence stability. In this work, we propose a novel light-scattering-based droplet screening (LSDS) that can effectively detect and characterize single cells confined in droplets by adjusting the optical properties of droplets in a multiangle optofluidic chip. Theoretical and simulated calculations suggest that refractive index (RI) matching in droplet two-phase materials can reduce or eliminate droplets’ scattered signals (background signal), enabling the differentiation of scattered signals from single cells and particles within droplets. Furthermore, by using a set of multiangle (from −145° to 140°) optical fibers integrated into the optofluidic chip, the scattered light properties of droplets with the RI ranging from 1.334 to 1.429 are measured. We find that the smaller the RI and size of microparticles inside droplets are, the smaller the RI difference between two-phase materials Δn is required. Especially, when Δn is smaller than 0.02, single cells in droplets can be detected and analyzed solely based on light scattering. This capability allows to accurately detect droplets containing one single cell and one single gel bead, a typical droplet encapsulation for single-cell sequencing. Altogether, this work provides a powerful platform for high-throughput label-free single-cell analysis in microdroplets for diverse single-cell related biological assays.

Transform-Limited Photon Emission from a Lead-Vacancy Center in Diamond above 10K.

Transform-limited photon emission from quantum emitters is essential for high-fidelity entanglement generation. In this Letter, we report the coherent optical property of a single negatively charged lead-vacancy (PbV) center in diamond. Photoluminescence excitation measurements reveal stable fluorescence with a linewidth of 39MHz at 6K, close to the transform limit estimated from the lifetime measurement. We observe 4 orders of magnitude different linewidths of the two zero-phonon lines, and find that the phonon-induced relaxation in the ground state contributes to this huge difference in the linewidth. Because of the suppressed phonon absorption in the PbV center, we observe nearly transform-limited photon emission up to 16K, demonstrating its high temperature robustness compared to other color centers in diamond.

In Situ Fabrication of Photoluminescent Hydrogen-Bonded Organic Framework-Functionalized Ca (II) Hydrogel Film for the Tetracyclines Visual Sensor and Information Security.

A facilely in situ fabricated hydrogen-bonded organic framework (HOF) hydrogel film with perfect photoluminescent performance was designed for visual sensing of tetracycline antibiotics (TCs) and information security. Luminescent HOF (MA-IPA) was combined with sodium alginate (SA) through hydrogen bonding actions and electrostatic interactions, then cross-linked with Ca2+ ions to form HOF hydrogel film (Ca@MA-IPA@SA). The HOF hydrogel film exhibited exceptional mechanical robustness along with stable blue fluorescence and ultralong green phosphorescence. After exposure to TCs, Ca2+ was combined with TCs to generate a new green fluorescence exciplex (TC-Ca2+) in hydrogel films. Due to fluorescence resonance energy transfer, the fluorescence of MA-IPA was quenched, and the fluorescent color of the HOF hydrogel film was changed from blue to green. This dichromatic fluorescent response is convenient for the visual and rapid detection of TCs. The detection limits of tetracycline (TC), oxytetracycline (OTC), and chlortetracycline (CTC) were 5.1, 7.7, and 32.7 ng mL-1, respectively. Importantly, this hydrogel sensing platform was free of tedious operation and enabled the ultrasensitive and selective detection of TCs within 6 min. It has been successfully applied to TC detection in pork and milk samples. Based on the stable photoluminescence performance of HOF hydrogel films and fluorescent-responsive properties to TCs, two types of anticounterfeiting arrays were fabricated for information encryption and decryption. This work provides a novel approach for on-site detection of TCs and offers valuable insights into information security.

Zinc(II)-MOF: A Versatile Luminescent Sensor for Selective Molecular Recognition of Flame Retardants and Antibiotics.

An exceptional Zinc(II)-organic framework with the formula [{Zn(L4-py)(bdc)}·DMF]n (Zn-MOF) has been constructed solvothermally using a novel linker L4-py {2,7-bis(3-(pyridin-4-ylethynyl)phenyl)benzo[lmn][3,8]phenanthroline-1,3,6,8(2H,7H)-tetraone}, coligand H2bdc (1,4-benzenedicarboxylic acid), and ZnBF4·xH2O. The ligand L4-py has been fabricated after functionalization of NDA (1,4,5,8-naphthalenetetracarboxylic dianhydride) core with 3-(pyridin-4-ylethynyl)phenyl group. The single-crystal X-ray analysis reveals that Zn-MOF exhibits a comprehensive three-dimensional (3D) framework architecture and features (4)-connected uninodal dia; 4/6/c1; sqc6 topology with point symbol {66} and two-dimensional (2D) + 2D, parallel polycatenation. Notably, Zn-MOF displayed excellent fluorescence phenomenon and stability in water as well as in methanol solvents and was harnessed as a versatile sensor, demonstrating selective and sensitive molecular recognition of flame retardants and antibiotics. Notably, Zn-MOF displayed 57 and 49.5% quenching efficiency for the flame-retardant pentabromophenol (PBP) and 3,3',5,5'-tetrabromobisphenol A (TBPA), respectively. Whereas an outstanding 90% quenching efficiency was observed for antibiotics, tetracycline (TC) and secnidazole (SD). The mechanistic investigations of this luminescence quenching suggest that this might be primarily occurring via the Fourier resonance energy transfer (FRET) and photoinduced electron transfer (PET) mechanisms, which might be assisted by the competitive absorption and host-guest interactions. The π-electron-rich framework structure of sensor Zn-MOF activates this mechanism.

Eu3+-Doped Anionic Zinc-Based Organic Framework Ratio Fluorescence Sensing Platform: Supersensitive Visual Identification of Prescription Drugs.

Advanced techniques for both environmental and biological prescription drug monitoring are of ongoing interest. In this work, a fluorescent sensor based on an Eu3+-doped anionic zinc-based metal-organic framework (Eu3+@Zn-MOF) was constructed for rapid visual analysis of the prescription drug molecule demecycline (DEM), achieving both high sensitivity and selectivity. The ligand 2-amino-[1,1'-biphenyl]-4,4'-dicarboxylic acid (bpdc-NH2) not only provides stable cyan fluorescence (467 nm) for the framework through intramolecular charge transfer of bpdc-NH2 infinitesimal disturbanced by Zn2+ but also chelates Eu3+, resulting in red (617 nm) fluorescence. Through the synergy of photoinduced electron transfer and the antenna effect, a bidirectional response to DEM is achieved, enabling concentration quantification. The Eu3+@Zn-MOF platform exhibits a wide linear range (0.25-2.5 μM) to DEM and a detection limit (LOD) of 10.9 nM. Further, we integrated the DEM sensing platform into a paper-based system and utilized a smartphone for the visual detection of DEM in water samples and milk products, demonstrating the potential for large-scale, low-cost utilization of the technology.

Investigation of the Application and Mechanism of Nitrogen and Phosphorus Co-doped Carbon Dots for Mercury Ion Detection.

In this paper, we obtained nitrogen and phosphorus co-doped carbon dots through a hydrothermal method using o-phenylenediamine and citric acid in a 40% phosphoric acid environment. The carbon dots emitted fluorescence at 476nm under excitation at 408nm and exhibited good selectivity and high sensitivity towards mercury ions. These carbon dots showed excellent dispersibility in water and maintained stable fluorescence even in high concentration salt environments. The interaction between mercury ions and functional groups on the carbon dots surface through electrostatic interaction resulted in static quenching. Simultaneously, by detecting the lifetime and transient absorption spectra of the carbon dots, we observed that the coordination of mercury ions with the carbon dots broadened the band structure of the carbon dots, and the existing photoinduced electron transfer process increased the non-radiative transition channel. The combined effect of dynamic quenching and static quenching significantly reduced the fluorescence intensity of the carbon dots at 476nm. The carbon dots exhibited linear detection of mercury ions in the range of 0.01-1 µM, with a detection limit as low as 0.0245 µM. In terms of practical water environmental detection applications, these carbon dots were able to effectively detect mercury ions in tap water and lake water, demonstrating their broad application prospects in the field of environmental metal analysis.

Enzyme-Responsive Modular Peptides Enhance Tumor Penetration of Quantum Dots via Charge Reversal Strategy.

Quantum dots (QDs) are colloidal semiconductor nanoparticles acting as fluorescent probes for detection, disease diagnosis, and photothermal and photodynamic therapy. However, their performance in cancer treatment is limited by inadequate tumor accumulation and penetration due to the larger size of nanoparticles compared to small molecules. To address this challenge, charge reversal nanoparticles offer an effective strategy to prolong blood circulation time and achieve enhanced endocytosis and tumor penetration. In this study, we leveraged the overexpressed γ-glutamyl transpeptidase (GGT) in many human tumors and developed a library of modular peptides to serve as water-soluble surface ligands of QDs. We successfully transferred the QDs from the organic phase to the aqueous phase within 5 min. And through systematic tuning of the peptide sequence, we optimized the fluorescent stability of QDs and their charge reversal behavior in response to GGT. The resulting optimal peptide stabilized QDs in aqueous solution with a high fluorescent retention rate of 93% after three months and realized the surface charge reversal of QDs triggered by GGT in vitro. The binding between the peptide and QD surface was investigated by using saturation transfer differential nuclear magnetic resonance (STD NMR). Thanks to its charge reversal ability, the GGT-responsive QDs exhibited enhanced cellular uptake in GGT-expressing cancer cells and deeper penetration in the 3D multicellular spheroids. This enzyme-responsive modular peptide can lead to specific tumor targeting and deeper tumor penetration, holding great promise to enhance the treatment efficacy of QD-based theranostics.

Spatial confinement of styryl pyridine salt derivative in MCM-22 molecular sieve network for boosted fluorescence emission and stable ratiometric sensing of bacillus anthracis biomarkers

In this work, a stable ratiometric nanofluorescent probe for the detection of 2,6-dipicolinic acid (DPA), a Bacillus anthracis biomarker, was developed based on confinement-induced emission enhancement of cationic styrylpyridine salt derivative L in MCM-22 molecular sieve pores. The cationic L and the lanthanide Tb3+ were loaded into the pores of the molecular sieve by electrostatic interaction with the negatively charged AlO4 tetrahedron unit, and L exhibited enhanced red fluorescence emission as a stable fluorescence reference mark in the nanoprobe platform due to the restricted molecular torsion of L in the pores of MCM-22. At the same time, the characteristic green fluorescence emission of Tb3+ can be excited by energy transfer due to the “antenna effect” of DPA. The prepared Tb-L@MCM-22 nanoprobe showed specific selectivity and stable fluorescence ratiometric detection of DPA in tap water, lake water, bovine serum and actual bacterial spores. Benefiting from the confinement-induced fluorescence enhancement effect of L in the MCM-22 molecular sieve pores, the obtained Tb-L@MCM-22 can provide a stable reference signal for the fluorescence ratiometric detection of DPA with a limit of detection (LOD) of 78.6 nM and 1.310 × 104 spores per mL. More importantly, combining of the Tb-L@MCM-22 based DPA detection test strips with a smartphone app demonstrated a stable, convenient and rapid method for detecting of anthrax biomarkers.

Engineering Tunable Ratiometric Dual Emission in Single Emitter-based Amorphous Systems.

Molecular emitters with multi-emissive properties are in high demand in numerous fields, while these properties basically depend on specific molecular conformation and packing. For amorphous systems, special molecular arrangement is unnecessary, but it remains challenging to achieve such luminescent behaviors. Herein, we present a general strategy that takes advantage of molecular rigidity and S1 -T1 energy gap balance for emitter design, which enables fluorescence-phosphorescence dual-emission properties in various solid forms, whether crystalline or amorphous. Subsequently, the amorphism of the emitters based polymethyl methacrylate films endowed an in situ regulation of the dual-emissive characteristics. With the ratiometric regulation of phosphorescence by external stimuli and stable fluorescence as internal reference, highly controllable luminescent color tuning (yellow to blue including white emission) was achieved. There properties together with a persistent luminous behavior is of benefit for an irreplaceable set of optical information combination, featuring an ultrahigh-security anti-counterfeiting ability. Our research introduces a concept of eliminating the crystal-form and molecular-conformational dependence of complex luminescent properties through emitter molecular design. This has profound implications for the development of functional materials.

Engineering Tunable Ratiometric Dual Emission in Single Emitter‐based Amorphous Systems

AbstractMolecular emitters with multi‐emissive properties are in high demand in numerous fields, while these properties basically depend on specific molecular conformation and packing. For amorphous systems, special molecular arrangement is unnecessary, but it remains challenging to achieve such luminescent behaviors. Herein, we present a general strategy that takes advantage of molecular rigidity and S1‐T1 energy gap balance for emitter design, which enables fluorescence–phosphorescence dual‐emission properties in various solid forms, whether crystalline or amorphous. Subsequently, the amorphism of the emitters based polymethyl methacrylate films endowed an in situ regulation of the dual‐emissive characteristics. With the ratiometric regulation of phosphorescence by external stimuli and stable fluorescence as internal reference, highly controllable luminescent color tuning (yellow to blue including white emission) was achieved. There properties together with a persistent luminous behavior is of benefit for an irreplaceable set of optical information combination, featuring an ultrahigh‐security anti‐counterfeiting ability. Our research introduces a concept of eliminating the crystal‐form and molecular‐conformational dependence of complex luminescent properties through emitter molecular design. This has profound implications for the development of functional materials.

Metal-organic framework modified molecularly imprinted polymers-based sensor for fluorescent sensing of tetracycline in milk

Tetracycline (TC) residues have a serious impact on human health, sustainable development of animal husbandry and aquaculture. Herein, a novel sensor (Cr-MOF@MIP) for TC detection was developed by modifying molecularly imprinted polymers (MIPs) on the surface of a luminescent metal-organic framework (MOFs). The sensor not only possesses excellent fluorescence stability and specific recognition sites, but also enables selective aggregation of the target. Subsequently, the physicochemical properties of Cr-MOF@MIP, such as morphological structure, crystal shape, surface area, pore size and functional groups, were characterized. Under the optimized conditions, the relative fluorescence intensity of the Cr-MOF@MIP fluorescent sensor decreased gradually and linearly as the TC concentration increased from 0.2 μg/mL to 50 μg/mL. The limit of detection (LOD) of TC was 0.032 μg/mL. In addition, the sensor has higher selectivity for TC compared to non-imprinted polymers (NIPs), with a bursting mechanism of Cr-MOF@MIP mainly attributed to the fluorescence resonance energy transfer. Remarkably, in the complex systems of milk, its specific detection of TC was demonstrated with satisfactory recoveries (90.06–104.34 %). It is expected that the development of Cr-MOF@MIP sensor will provide a new way of thinking for easy, rapid, highly selective and cost-effective analysis in complex samples.

Fluorescent Eu-MOF@nanocellulose-based nanopaper for rapid and sensitive detection of uranium (Ⅵ)

Radioactive uranium leaks into natural water bodies mainly in the form of uranyl ions (UO22+), posing ecological and human health risks. Fluorescent europium-based metal-organic frameworks (Eu-MOFs) have been demonstrated to be effective fluorescent sensors for UO22+, but the large size, powder state and poor dispersity limit their further application. In this work, fluorescent Eu-MOFs were in-situ grown on TEMPO-oxidized cellulose nanofibers (TOCNFs), which is the first time that spherical Eu-MOF crystals with sizes below 10 nm were prepared. Fluorescence spectral analysis revealed a nine-fold increase in the fluorescence intensity of TOCNF@Eu-MOF compared to Eu-MOF. The nanocomposites achieved rapid and sensitive fluorescence quenching to UO22+ through the “antenna effect” and unsaturated Lewis basic sites on the ligands binding with UO22+. Moreover, TOCNF@Eu-MOF demonstrated excellent selectivity and anti-interference for UO22+ detection. For the nanopaper-based sensor made from TOCNF@Eu-MOF, the Stern-Volmer quenching constant (KSV) was calculated as 8.21 × 104 M−1, and the lowest limit of detection (LOD) was 6.6 × 10−7 M, significantly lower than the 1.32 × 10−6 M of Eu-MOFs. In addition, the nanopaper exhibited good fluorescence stability and cyclic detection performance, enabling the rapid and convenient detection of UO22+ in the aqueous phase within 30 s by simple dipping.

3D PRINTED POLYETHER-ETHER-KETONE (PEEK) MECHANICAL-ADAPTIVE IMPLANTS FOR IMMUNOMODULATORY OSSEOINTEGRATION

3D Printed polyether-ether-ketone (PEEK) has gained widespread use in clinical practice due to its excellent biocompatibility, biomechanical compatibility, and personalization. However, pre-printed PEEK implants are not without their flaws, including bioinert, optimization distortion of 3D printing digital model and prosthetic mismatching. Recent advancements in mechanical processing technology have made it possible to print bone implants with PEEK fused deposition, allowing for the construction of mechanically adaptable implants. In this study, we aimed to synthesize silanized polycitrate (PCS) via thermal polymerization and in situ graft it to PEEK surface to construct an elastomer coating for 3D printed PEEK implants (PEEK-PCS). This incorporation of PCS allows the implant to exhibit adaptive space filling ability and stress dispersal. In vivo and in vitro results, PEEK-PCS exhibited exceptional osseointegration and osteogenesis properties along with macrophage M2 phenotypic polarization, inflammatory factors reducing, promotion of osteogenic differentiation in bone marrow mesenchymal stem cells (BMSCs). Additionally, PEEK-PCS displays good autofluorescence properties in vitro and in vivo, with stable fluorescence for 14 days, suggesting potential bioimaging applications. The study confirms that PEEK in situ grafting with thermo-polymerized PCS elastomers is a viable approach for creating multifunctional (bone defect adaptation, bioimaging, immune regulation, and osseointegration) implants for bone tissue engineering.

Facile synthesis of sulphur-doped carbon dots (S-CDs) using a hydrothermal method for the selective sensing of Cr6+ and Fe3+ ions: application to environmental water sample analysis.

In this work, we used a one-step hydrothermal method to synthesize blue-emission sulfur-doped carbon dots (S-CDs) using jaggery as a carbon precursor. The synthesized carbon quantum dots showed low toxicity, good water solubility, anti-interference properties, and stable fluorescence. When excited at 310 nm, the S-CDs produced bright emission with a quantum yield of 7.15% at 397 nm. The S-CDs exhibited selective and sensitive quenching responses with limits of detection (LODs) of 4.25 μg mL-1 and 3.15 μg mL-1 for variable concentrations of Cr6+ and Fe3+, respectively, accompanied by a consistent linear relationship between fluorescence intensity and these concentrations. Fluorescence lifetime measurements were used to investigate the fluorescence quenching mechanism, which supports the static type of quenching. Outstanding benefits of the developed S-CD based fluorescence probe include its low cost, excellent sensitivity and selectivity, and ease of use for the detection of Cr6+ and Fe3+ ions. The developed carbon dot based fluorescent probe was successfully used to detect Cr6+ and Fe3+ ions in real water samples with an excellent recovery ratio.

Pyrene fluorescence in 2,7-di(4-phenylethynyl)pyrene-bridged bis(alkenylruthenium) complexes.

Complexes PyrDPE-RuCl and PyrDPE-Ruacac with a π-extended 2,7-di(4-phenylethynyl)pyrene linker undergo simultaneous one-electron oxidations of their {Ru}-styryl entities. The absence of an intervalence charge-transfer (IVCT) band at intermediate stages, where the mixed-valent, singly oxidized radical cation is present, and spin density confinement to the terminal styryl ruthenium site(s) are tokens of a lack of electronic coupling between the {Ru} entities across the π-conjugated linker. The close similarity of the linker-based π → π* bands in the complexes and the free ligand and their insensitivity towards oxidations at the terminal sites indicate that the central pyrenyl fluorophore is electronically decoupled from the electron-rich {Ru}-styryl termini. As a consequence, the complexes offer stable pyrene-based fluorescence emissions at 77 K, which are red-shifted from that of the linker.

Nitrogen-doped biomass-derived carbon dots for fluorescence determination of sunset yellow.

Sunset Yellow (SY) is a widely used food coloring in the food industry. However, exceeding the allowable limit of this dye poses a significant threat to human health. To address this issue, we developed Lycium ruthenicum-derived nitrogen-doped carbon dots (N-CDs) with a stable blue fluorescence through hydrothermal treatment for SY determination. The quantum yield (QY) of these N-CDs was found to be up to 10.63%. Physical characterization of N-CDs was performed using various spectroscopic techniques to confirm their excellent photostability and non-toxic properties. Furthermore, the presence of SY had a substantial quenching effect on the fluorescence intensity (F0/F) of the N-CDs. Leveraging this observation, we developed a fluorescent sensor for the determination of SY in the concentration range of 0.05 to 35.0 μM, with a limit of detection (LOD, 3σ/K) of 17 nM. The excellent fluorescent sensor also showed satisfactory results in the practical drink samples. Moreover, the stability and cytotoxicity of N-CDs as a fluorescent probe were studied. Finally, the N-CDs were applied to cell imaging using A549 cells.

Trifluoroethylamine-substituted solvatochromic fluorophores exhibit polarity-insensitive high brightness.

In this study, we have uncovered that trifluoroethylamine-substituted solvatochromic fluorophores maintain consistently high and stable fluorescence intensity in diverse polar environments, including highly polar and protic solvents. The 1,8-naphthalimide derivatives serve as a buffering fluorogenic indicator for lipid droplet morphology during the fusion process and ratiometric probe for microenvironment polarity based on Halo-tag technology.

One-pot hydrothermal synthesis of fluorophore-modified cerium oxide nanoparticles.

Cerium oxide nanoparticles (CeO2 NPs), which have powerful antioxidant properties, are promising nanomaterials for the treatment of diseases associated with oxidative stress. The well-developed surface of CeO2 NPs makes them promising for use as a multifunctional system for various biomedical applications. This work demonstrates a simple approach that allows the direct formation of a molecular fluorophore on the surface of CeO2 NPs using a simple one-pot hydrothermal synthesis. Thus, we were able to synthesize CeO2 NPs of ultra-small size ∼2 nm with a narrow distribution, highly stable fluorescence, and a quantum yield of ∼62%. UV-visible transmission studies revealed that the resulting CeO2 NPs exhibited fast autogenerative catalytic reduction. In vitro results showed high biocompatibility of CeO2 NPs; their internalization occurs mainly in the region of cell nuclei. Thus, the resulting NPs have the necessary parameters and can be successfully used in biovisualization and therapy.