Adding biochar to soils is being advocated to mitigate soil erosion. However, given biochar aging and persistency in soils, the effects of biochar on soil in the long-term are important to investigate yet remain largely unknown. The objective was therefore to determine the long-term effect of biochar on interrill erosion on cropland soils. Experiments were conducted using topsoil from three fields of different textures (silt loam, loam and sandy loam). Each field was characterized by the presence of kiln sites containing century-old charcoal used as proxy to investigate the long-term impact of biochar. Aggregate stability was measured using the method of Le Bissonnais (1996), whereas interrill erosion was studied using two successive (24-h apart) rainfall simulation experiments (84 mm h−1, 90 min.). The presence of historical charcoal in kiln sites did not affect soil aggregate stability, irrespective of charcoal-C content or soil type. For the first rainfall simulation only, the century-old charcoal reduced both the final runoff and soil loss rates by, respectively, 15.4 mm h−1 and 12.1 g m−2 h−1 for each percent increase in charcoal-C content. As a result, Meyer’s interrill erodibility decreased with increasing charcoal-C content. However, because the final runoff and soil loss rates decreased to a similar extent with increasing charcoal-C content, Kinnell’s interrill erodibility was unaffected by charcoal-C content. These effects on interrill erosion parameters were independent of soil type and could not be related to changes in soil chemical properties. The measured reduction in runoff and soil loss were attributed to delayed crust development, which could be related to increasing proportions of clods in the soil samples with increasing charcoal-C content. Further research may be needed to confirm the observed trends and underlying mechanisms over a wider range of soil types.