Effects of long-term application of inorganic fertilizers and organic amendments on the turnover rates of fractionated soil organic carbon and their determining factors in paddy fields

Abstract

ABSTRACT To investigate how the long-term application of inorganic fertilizer and organic amendments affects the turnover rates of organic matter in physico-chemically fractionated components in paddy soils and how their rates are related to their amounts, we studied the effect of variable management of inorganic fertilizer and organic amendments for > 50 years on the ∆14C values of accumulated soil organic carbon (SOC) in fractionated components of 5 soil samples from three paddy fields. SOC was fractionated into four components based on their physico-chemical properties: (1) light fraction (LF) derived from plant residues, (2) heavy fraction (HF) containing stable aggregates, (3) oxidizable fraction (OxF) and (4) non-oxidizable fraction (NOxF) forming organo-mineral complexes with fine-textured minerals. The ∆14C values were determined by accelerator mass spectrometry using graphitized samples. The ∆14C values of SOC in NOxF (from −305‰ to − 90.5‰) were much lower than those of HF (from −123‰ to − 3.7‰) and OxF (from −215‰ to 21.0‰). Lowest values for NOxF suggested slowest turnover rates as expected, and higher and comparable values for OxF and HF implied much faster turnover rates of SOC in OxF compared with upland soils. This could be one of the characteristics of SOC dynamics in paddy soils, probably reflecting repeated puddling of plow layer soils. ∆14C values in all the fractions increased in response to the application of inorganic fertilizer and organic amendments, suggesting faster turnover rate. Soils with clayey and weathered characteristics had the lowest ∆14C values, or slowest turnover rates among the study fields. The relationship between the ∆14C value and the amount of accumulated C for HF, OxF and NOxF indicated that the more the SOC accumulated, the faster the turnover rate of the SOC was, regardless of the fractions, and the relationship of the HF was considerably different from those of OxF and NOxF. In conclusion, these results can be the basis for understanding the C dynamics and for improving soil fertility status and soil C sequestration in paddy soils.