Stability Of Condensate Research Articles

Excitonic condensation state in the assistance of the adiabatic and anti-adiabatic phonons

The excitonic condensate in semimetal and semiconducting materials with the assistance of the phonons in both the adiabatic and anti-adiabatic regimes has been investigated. In the framework of the random phase approximation, we analyse the static excitonic susceptibility function once the electrons and holes are described in the extended Falicov–Kimball model involving the electron–phonon interaction. The excitonic condensate transition point is detected as a divergence of the susceptibility function. The complex phase diagrams exhibit the significant impact of the adiabatic phonons on the stability of the excitonic condensation state. In contrast, phonons in the anti-adiabatic regime play a less role in the formation and the condensation of the excitons. Depending on the temperature or the external pressure, the excitonic condensate stability and its Bardeen–Cooper–Schrieffer–Bose–Einstein condensation crossover in the systems are also discussed in detail.

RNA chain length and stoichiometry govern surface tension and stability of protein-RNA condensates

RNA chain length and stoichiometry govern surface tension and stability of protein-RNA condensates

RNA length has a non-trivial effect in the stability of biomolecular condensates formed by RNA-binding proteins.

Biomolecular condensates formed via liquid–liquid phase separation (LLPS) play a crucial role in the spatiotemporal organization of the cell material. Nucleic acids can act as critical modulators in the stability of these protein condensates. To unveil the role of RNA length in regulating the stability of RNA binding protein (RBP) condensates, we present a multiscale computational strategy that exploits the advantages of a sequence-dependent coarse-grained representation of proteins and a minimal coarse-grained model wherein proteins are described as patchy colloids. We find that for a constant nucleotide/protein ratio, the protein fused in sarcoma (FUS), which can phase separate on its own—i.e., via homotypic interactions—only exhibits a mild dependency on the RNA strand length. In contrast, the 25-repeat proline-arginine peptide (PR25), which does not undergo LLPS on its own at physiological conditions but instead exhibits complex coacervation with RNA—i.e., via heterotypic interactions—shows a strong dependence on the length of the RNA strands. Our minimal patchy particle simulations suggest that the strikingly different effect of RNA length on homotypic LLPS versus RBP–RNA complex coacervation is general. Phase separation is RNA-length dependent whenever the relative contribution of heterotypic interactions sustaining LLPS is comparable or higher than those stemming from protein homotypic interactions. Taken together, our results contribute to illuminate the intricate physicochemical mechanisms that influence the stability of RBP condensates through RNA inclusion.

RNA-induced modulation of the stability and transport properties of protein biomolecular condensates: on the two sides of the same coin

Liquid-liquid phase separation (LLPS) has been demonstrated to be a key process in regulating cell compartmentalization. The emergence of phase-separated biomolecular condensates is effectively sustained by proteins with intrinsically disordered regions that can establish multiple homotypic self-interactions or heterotypic interactions with other biomolecules. Particularly, RNA-binding protein (RBP) phase separation has been shown to be critically regulated by RNA, and even though misregulation of LLPS is deeply connected to the onset of several neurodegenerative disorders such as Alzhemier, ALS, or even some types of cancer, the underlying molecular aspects remain hardly unclear.

Surface Electrostatics Govern the Emulsion Stability of Biomolecular Condensates.

Liquid-liquid phase separation underlies the formation of biological condensates. Physically, such systems are microemulsions that in general have a propensity to fuse and coalesce; however, many condensates persist as independent droplets in the test tube and inside cells. This stability is crucial for their function, but the physicochemical mechanisms that control the emulsion stability of condensates remain poorly understood. Here, by combining single-condensate zeta potential measurements, optical microscopy, tweezer experiments, and multiscale molecular modeling, we investigate how the nanoscale forces that sustain condensates impact their stability against fusion. By comparing peptide-RNA (PR25:PolyU) and proteinaceous (FUS) condensates, we show that a higher condensate surface charge correlates with a lower fusion propensity. Moreover, measurements of single condensate zeta potentials reveal that such systems can constitute classically stable emulsions. Taken together, these results highlight the role of passive stabilization mechanisms in protecting biomolecular condensates against coalescence.

Dynamics of Bose-Einstein condensates under anharmonic trap

The dynamics of weakly interacting three-dimensional Bose-Einstein condensates (BECs), trapped in external axially symmetric plus anharmonic distortion potential are studied. Within a variational approach and time-dependent Gross-Pitaevskii equation, the coupled condensate width equations are derived. By modulating anharmonic distortion of the trapping potential, nonlinear features are studied numerically and illustrated analytically, such as mode coupling of oscillation modes, and resonances. Furthermore, the stability of attractive interaction BEC in both repulsive and attractive anharmonic distortion is examined. We demonstrate that a small repulsive and attractive anharmonic distortion is effective in reducing (extending) the condensate stability region since it decreases (increases) the critical number of atoms in the trapping potential.

Exergy, Energy, Economic, and Environmental Assessment of Condensate Stabilization Unit Through Advanced Process Control: Application of Real-Time Optimization

Exergy, Energy, Economic, and Environmental Assessment of Condensate Stabilization Unit Through Advanced Process Control: Application of Real-Time Optimization

Stability of pyrolysis condensates during their high-temperature treatment

As part of a project dealing with the material use of waste plastics processed by pyrolysis, a method for the purification of primary pyrolysis gas at temperatures above the dew point of condensing components was proposed. In order to avoid the loss of liquid products, two procedures have been proposed to study this issue. The first procedure consists in separating the pyrolysis condensate from permanent gases and its subsequent evaporation and introduction into a high-temperature reactor where the purification takes place. The second procedure used the same equipment, but the pyrolyser was connected in series with a high temperature reactor by a heated tube. The function of the device is demonstrated on a pair of pure polymers, namely highdensity polyethylene and polypropylene. In practice, however, the device is used for testing waste plastics. The mass balance of liquid, gaseous and solid products of pyrolysis and subsequent vapour phase conduction through a high-tem-perature reactor was supplemented by data from chromatographic analysis. Experiments have shown that the separation of pyrolysis and subsequent evaporation of the condensate in an independent reactor causes the formation of an undesirable amount of fine aerosol (mist). Pyrolysis without any subsequent high-temperature step produced 85–90 % condensate. The inclusion of a separate high-temperature reactor reduced the yield of condensate to 44.5–47.5 %, at the expense of the above-mentioned mist. Its conver-sion back to liquid is difficult and makes the process inefficient for industry. In tests with the series-connected pyrolyser and the high-temperature reactor, the situation was significantly better. 68.5–73.5 % of condensate was obtained in this case. In addition to the formation of mist, the conduction of steam of condensing components through the high-temperature reactor also caused a slight change in the composition of the liquids obtained. There was a decrease in the proportion of C21–C29 hydrocarbons in products and, conversely, an increase in the concentration of C5–C15 hydrocarbons. Besides verifying a suitable approach to the high-temperature processing of pyrolysis products, the experiments showed that changing a single subparameter (in this case the separation of the two reactors) significantly altered the results of the experiments. During laboratory simulation of industrial processes, it is important not to approach simplifications, but to copy all conditions as much as possible.

‘RNA modulation of transport properties and stability in phase-separated condensates

One of the key mechanisms employed by cells to control their spatiotemporal organization is the formation and dissolution of phase-separated condensates. The balance between condensate assembly and disassembly can be critically regulated by the presence of RNA. In this work, we use a chemically-accurate sequence-dependent coarse-grained model for proteins and RNA to unravel the impact of RNA in modulating the transport properties and stability of biomolecular condensates. We explore the phase behavior of several RNA-binding proteins such as FUS, hnRNPA1, and TDP-43 proteins along with that of their corresponding prion-like domains and RNA recognition motifs from absence to moderately high RNA concentration. By characterizing the phase diagram, key molecular interactions, surface tension, and transport properties of the condensates, we report a dual RNA-induced behavior: on the one hand, RNA enhances phase separation at low concentration as long as the RNA radius of gyration is comparable to that of the proteins, whereas at high concentration, it inhibits the ability of proteins to self-assemble independently of its length. On the other hand, along with the stability modulation, the viscosity of the condensates can be considerably reduced at high RNA concentration as long as the length of the RNA chains is shorter than that of the proteins. Conversely, long RNA strands increase viscosity even at high concentration, but barely modify protein self-diffusion which mainly depends on RNA concentration and on the effect RNA has on droplet density. On the whole, our work rationalizes the different routes by which RNA can regulate phase separation and condensate dynamics, as well as the subsequent aberrant rigidification implicated in the emergence of various neuropathologies and age-related diseases.

Study of Zero Temperature Ground State Properties of the Repulsive Bose-Einstein Condensate in an Anharmonic Trap

The zero-temperature ground state properties of experimental 87Rb condensate are studied in a harmonic plus quartic trap [ V(r) = ½mω2r2 + λr4 ]. The anharmonic parameter (λ) is slowly tuned from harmonic to anharmonic. For each choice of λ, the many-particle Schrödinger equation is solved using the potential harmonic expansion method and determines the lowest effective many-body potential. We utilize the correlated two-body basis function, which keeps all possible two-body correlations. The use of van der Waals interaction gives realistic pictures. We calculate kinetic energy, trapping potential energy, interaction energy, and total ground state energy of the condensate in this confining potential, modelled experimentally. The motivation of the present study is to investigate the crucial dependency of the properties of an interacting quantum many-body system on λ. The average size of the condensate has also been calculated to observe how the stability of repulsive condensate depends on anharmonicity. In particular, our calculation presents a clear physical picture of the repulsive condensate in an anharmonic trap.

Reduction of gas and aerosol pollution of atmospheric air at a condensate stabilization units

There is a wide variety of uses for non-compliant hydrocarbon products in addition to utilization by flaring, but the implementation of each of them is accompanied by a number of regulatory and technological restrictions. The paper analyzes the influence of the flare system on the state of the atmospheric air in the area of one of the gas condensate production facilities in Western Siberia. A detailed analysis of the composition of the combusted gas mixture is carried out, a review of existing methods of using non-compliant hydrocarbon gas raw materials is made. The technical substantiation of the possibility of introducing a gas turbine units for power generation with the stage of intermediate gas accumulation in a soft gas tank of variable volume and also with a waste gas cleaning system to reduce the impact on the atmospheric air has been carried out. A detailed ecological and economic substantiation of the proposed measures is given.

Dropwise condensation of acetone and ethanol for a high-performance lubricant-impregnated thermosyphon

A gravity-assisted heat pipe called a two-phase closed thermosyphon (TPCT) is an efficient thermal device that transfers a large amount of heat at a relatively small temperature difference using the phase-change process. Promoting dropwise condensation on a condenser wall of a TPCT promises an increase in the effective thermal conductance of that device. Acetone and ethanol have been widely used for heat pipe applications as working fluids. Still, they pose a limitation in promoting dropwise condensation using typical hydrophobic coatings due to their lower surface energies. Recent works have shown that lubricant-impregnated surfaces (LIS) can promote dropwise condensation of low-surface-tension fluids, but no studies proved the effectiveness of the surfaces in TPCT applications. This study experimentally achieves dropwise condensation of acetone and ethanol within a TPCT using the LIS surface. We demonstrate heat transfer enhancement of a TPCT by examining temperature uniformity, condenser and evaporator heat transfer coefficients (HTCs), and overall and partial thermal resistances. We demonstrated that the LIS condenser increased the condenser HTC by 220% and decreased the overall thermal resistance of the TPCT by 44% compared to filmwise condensation. Our work also revealed a strong dependence of working fluid viscosity on dropwise condensation stability and heat transfer enhancement by comparing the two working fluids, acetone, and ethanol. This work presents the opportunity for improving thermal performance in heat pipes and applications that rely on the condensation of low-surface-tension fluids, such as HVAC, refrigeration, and organic Rankine cycle.

Stability and superfluidity of the Bose-Einstein condensate in a two-leg ladder with magnetic field.

The stability and superfluidity of the Bose-Einstein condensate in two-leg ladder with magnetic field are studied. The dispersion relation and the phase diagram of the system are obtained. Three phases are revealed: the Meissner phase, the biased ladder (BL) phase, and the vortex phase. The dispersion relation and phase transition of the system strongly depend on the magnitude of atomic interaction strength, the rung-to-leg coupling ratio and the magnetic flux. Particularly, the change of the energy band structure in the phase transition region is modified significantly by the atomic interaction strength. Furthermore, based on the Bogoliubov theory, the energetic and dynamical stability of the system are invested. The stability phase diagram in the full parameter space is presented, and the dependence of superfluidity on the dispersion relation is illustrated explicitly. The atomic interaction strength can produce dynamical instability in the energetic unstable region and can expand the superfluid region. The results show that the stability of the system can be controlled by the atomic interaction strength, the rung-to-leg coupling ratio and the magnetic flux. In addition, the excitation spectrums in the Meissner phase, BL phase and vortex phase are further studied. The modulation of the excitation spectrum and the energetic stability of the system by the atomic interaction strength, the rung-to-leg coupling ratio and magnetic flux is discussed. Finally, through the numerical simulation, the dynamical instability of the system is verified by the time evolution of the Bloch wave and rung current. This provides a theoretical basis for controlling the superfluidity of the system.

A reliability prediction methodology based on improved radial basis function neural network (RBFNN): a condensate light crude oil stabilization facility as case study

In this article, a risk analysis was carried out on condensate stabilisation facility. The condensate or light crude oil produced from oil and gas facilities can be highly volatile i.e. the liquid can vaporise very easily, forming a flammable atmosphere, thereby making it difficult or unsafe for storage in atmospheric tanks. Due to its volatility, a high vapour pressure can result in seal damage to floating roof tanks with the potential to result in roof collapse and/or loss of containment of the stored liquid to atmosphere (on top of the roof). The Radial Basis Function Neural Network (RBFNN) proposed method herein is based on clustering of input space vectors and computing weights of Euclidian distances; histogram equalisation within each cluster will determine the centre and width of each receptive field for the estimation of the risk parameters. The RBFNN is designed to estimate the quality of performing consistently well (Reliability) to minimise any risk in the tank. The obtained results demonstrate the high performances of the proposed method where the deviation between Weibull function and the predicted value using RBFNN is less than 0.5%.

Do polyproline II helix associations modulate biomolecular condensates?

Biomolecular condensates are microdroplets that form inside cells and serve to selectively concentrate proteins, RNAs and other molecules for a variety of physiological functions, but can contribute to cancer, neurodegenerative diseases and viral infections. The formation of these condensates is driven by weak, transient interactions between molecules. These weak associations can operate at the level of whole protein domains, elements of secondary structure or even moieties composed of just a few atoms. Different types of condensates do not generally combine to form larger microdroplets, suggesting that each uses a distinct class of attractive interactions. Here, we address whether polyproline II (PPII) helices mediate condensate formation. By combining with PPII‐binding elements such as GYF, WW, profilin, SH3 or OCRE domains, PPII helices help form lipid rafts, nuclear speckles, P‐body‐like neuronal granules, enhancer complexes and other condensates. The number of PPII helical tracts or tandem PPII‐binding domains can strongly influence condensate stability. Many PPII helices have a low content of proline residues, which hinders their identification. Recently, we characterized the NMR spectral properties of a Gly‐rich, Pro‐poor protein composed of six PPII helices. Based on those results, we predicted that many Gly‐rich segments may form PPII helices and interact with PPII‐binding domains. This prediction is being tested and could join the palette of verified interactions contributing to biomolecular condensate formation.

Optimization of Energy Consumption in the Process of Dehumidification of Natural Gas

In recent years, there has been a great tendency to optimize energy consumption in the oil and gas industry’s upstream and downstream equipment. One of the most energy-intensive processes in natural gas refineries is the condensate stabilization unit (gas condensate). The main bottlenecks of energy consumption in the old units are condensated stabilization (dehumidification with ethylene glycol), heater reboiler, and air coolers (air coolers). Therefore, much attention should be paid to these applications and electricity and steam consumption in this unit. In this study, a simulated model based on the Gachsaran gas refinery’s new layout has been developed. Optimization of this part of the existing process is preheating the inlet flow to the reboiler by adding a two-stage shell-tube heat exchanger. This reduces the amount of steam needed to evaporate the inlet stream to the end of the tower. On the other hand, by pre-cooling the inlet currents to the air conditioners, the amount of electricity consumed to reach the outlet flows’ the desired temperature would be reduced. The results show an attractive return on investment for the remediation plan, a reduction in energy demand, and an increase in the unit’s productivity.

Temperature-Responsive Peptide-Nucleotide Coacervates.

Coacervates are a type of liquid–liquid phase separated (LLPS) droplets that can serve as models of membraneless organelles (MLOs) in living cells. Peptide–nucleotide coacervates have been widely used to mimic properties of ribonucleoprotein (RNP) granules, but the thermal stability and the role of base stacking is still poorly understood. Here, we report a systematic investigation of coacervates formed by five different nucleoside triphosphates (NTPs) with poly-l-lysine and poly-l-arginine as a function of temperature. All studied combinations exhibit an upper critical solution temperature (UCST), and a temperature-dependent critical salt concentration, originating from a significant nonelectrostatic contribution to the mixing free energy. Both the enthalpic and entropic parts of this nonelectrostatic interaction decrease in the order G/A/U/C/T, in accordance with nucleobase stacking free energies. Partitioning of two dyes proves that the local hydrophobicity inside the peptide–nucleotide coacervates is different for every nucleoside triphosphate. We derive a simple relation between the temperature and salt concentration at the critical point based on a mean-field model of phase separation. Finally, when different NTPs are mixed with one common oppositely charged peptide, hybrid coacervates were formed, characterized by a single intermediate UCST and critical salt concentration. NTPs with lower critical salt concentrations can remain condensed in mixed coacervates far beyond their original critical salt concentration. Our results show that NTP-based coacervates have a strong temperature sensitivity due to base stacking interactions and that mixing NTPs can significantly influence the stability of condensates and, by extension, their bioavailability.

Biophysics of Phase Separation of Disordered Proteins Is Governed by Balance between Short- And Long-Range Interactions.

Intrinsically disordered proteins play a crucial role in cellular phase separation, yet the diverse molecular forces driving phase separation are not fully understood. It is of utmost importance to understand how peptide sequence, and particularly the balance between the peptides’ short- and long-range interactions with other peptides, may affect the stability, structure, and dynamics of liquid–liquid phase separation in protein condensates. Here, using coarse-grained molecular dynamics simulations, we studied the liquid properties of the condensate in a series of polymers in which the ratio of short-range dispersion interactions to long-range electrostatic interactions varied. As the fraction of mutations that participate in short-range interactions increases at the expense of long-range electrostatic interactions, a significant decrease in the critical temperature of phase separation is observed. Nevertheless, sequences with a high fraction of short-range interactions exhibit stabilization, which suggests compensation for the loss of long-range electrostatic interactions. Decreased condensate stability is coupled with decreased translational diffusion of the polymers in the condensate, which may result in the loss of liquid characteristics in the presence of a high fraction of uncharged residues. The effect of exchanging long-range electrostatic interactions for short-range interactions can be explained by the kinetics of breaking intermolecular contacts with neighboring polymers and the kinetics of intramolecular fluctuations. While both time scales are coupled and increase as electrostatic interactions are lost, for sequences that are dominated by short-range interactions, the kinetics of intermolecular contact breakage significantly slows down. Our study supports the contention that different types of interactions can maintain protein condensates, however, long-range electrostatic interactions enhance its liquid-like behavior.

Thermodynamics and kinetics of phase separation of protein-RNA mixtures by a minimal model

Intracellular liquid-liquid phase separation enables the formation of biomolecular condensates, such as ribonucleoprotein granules, which play a crucial role in the spatiotemporal organization of biomolecules (e.g., proteins and RNAs). Here, we introduce a patchy-particle polymer model to investigate liquid-liquid phase separation of protein-RNA mixtures. We demonstrate that at low to moderate concentrations, RNA enhances the stability of RNA-binding protein condensates because it increases the molecular connectivity of the condensed-liquid phase. Importantly, we find that RNA can also accelerate the nucleation stage of phase separation. Additionally, we assess how the capacity of RNA to increase the stability of condensates is modulated by the relative protein-protein/protein-RNA binding strengths. We find that phase separation and multiphase organization of multicomponent condensates is favored when the RNA binds with higher affinity to the lower-valency proteins in the mixture than to the cognate higher-valency proteins. Collectively, our results shed light on the roles of RNA in ribonucleoprotein granule formation and the internal structuring of stress granules.

Improving a process design for the condensate stabilization unit

The condensate stabilization process is known as a common operation in gas fields with the aim of diminishing the condensate Reid Vapor Pressure (RVP). Based on feed characteristics and specified products, condensate stabilization units can be optimized by examining different process configurations. In each configuration, there exist some criteria in terms of product quality, energy saving and economic recovery, which shall be investigated so that the configuration’s performance is evaluated and selected. The aim of this study is to illustrate four condensate stabilization configurations and then to determine the proper column temperature and pressure, feed split ratio and feed tray location by means of Aspen Hysys in the configurations. In the end, it is shown that the configuration with the lowest operating pressure not only had the lowest fixed capital cost, but also consumed the lowest energy due to its high separation efficiency and low energy requirement.