The selection of the right host plant needs attention because of the interaction between the host plant, the type of AMF, the composition of the media, and the climate during its growth. It has been reported that in the C4 group, the best type of host plant used in producing AMF spores is Zea mays, while in the C3 group, Pueraria javanica is able to produce optimum AMF development, so a study is needed to combine C4 and C3 groups as hosts plant in AMF spore propagation. The study was arranged using a completely randomized design with 5 combination treatments of host plant groups C4 and C3, to determine the productivity of the host plant combination, the parameters observed were percentage of infected roots, abundance of mycorrhizal spores, and diameter of spore. The results showed that good host plant productivity was obtained from host plant combinations of Zea mays and Vigna angularis or Vigna unguiculata or Phaseolus vulgaris, where mycorrhizae can infect roots above 90% and produce spores that have a diameter of 300-400 µm, so it can be recommended that a host plant combination that has a fast life cycle is better used to stimulate the growth and regeneration of mycorrhizal spores, as a novelty in this study.