Spontaneous Interaction Research Articles

Integrated spectroscopic and computational analyses unravel the molecular interaction of pesticide azinphos-methyl with bovine beta-lactoglobulin.

Organophosphorus are typically hazardous chemicals used in the pharmaceutical, agricultural, and other industries. They pose a serious risk to human life and can be fatal upon direct exposure. Hence, studying the interaction between such compounds with proteins is crucial for environmental, health, and food safety. In this study, we investigated the interaction mechanism between azinphos-methyl (AZM) and β-lactoglobulin (BLG) at pH 7.4 using a combination of biophysical techniques. Intrinsic fluorescence investigations revealed that BLG fluorescence was quenched in the presence of increasing AZM concentrations. The quenching mechanism was identified as static, as evidenced by a decrease in the fluorescence quenching constant (1.25 × 104, 1.18 × 104, and 0.86 × 104 M-1) with an increase in temperatures. Thermodynamic calculations (ΔH > 0; ΔS > 0) affirmed the formation of a complex between AZM and BLG through hydrophobic interactions. The BLG's secondary structure was found to be increased due to AZM interaction. Ultraviolet -visible spectroscopy data showed alterations in BLG conformation in the presence of AZM. Molecular docking highlighted the significant role of hydrophobic interactions involving residues such as Val43, Ile56, Ile71, Val92, Phe105, and Met107 in the binding between BLG and AZM. A docking energy of -6.9 kcal mol-1, and binding affinity of 1.15 × 105 M-1 suggest spontaneous interaction between AZM and BLG with moderate to high affinity. These findings underscore the potential health risks associated with the entry of AZM into the food chain, emphasizing the need for further consideration of its impact on human health.

Detection of abnormal behaviors in prenatal Poly(I:C) exposed mice in a group-rearing environment.

During pregnancy, the maternal environment is critical for normal ontogeny and central nervous system development. Occasionally, prenatal exposure to environmental factors affects tissue architecture and functional development of the brain, which causes developmental disorders, including disorders of the autism spectrum. One of these environmental factors is the exposure to infectious diseases during pregnancy. In this study, we generated mice with infectious disease-induced inflammation by prenatal exposure to 200 μg/kg polyinosinic-polycytidylic acid sodium salt [Poly(I:C)] at embryonic day 12.5 and analyzed their phenotypes on 30-weeks-old. We attempted to detect abnormalities in spontaneous activity and social interaction, which may be indicators of developmental disorder-like behavioral abnormalities, in free-ranging behaviors in multiple rearing environments using multiple animal positioning systems and UMATracker in mice with fetal inflammation. Increased spontaneous activity and abnormal social interactions were observed in mice in the Poly(I:C)-treated group compared with those in the control group. Prenatal exposure to Poly(I:C) increased motor activity and decreased social interaction, and social behavior in prenatally treated mice in a multiple-individual rearing environment. Poly(I:C) exposure during the fetal period resulted in developmental disorder-like behavioral abnormalities, such as increased activity and abnormal social interactions, even after maturation in a multiple-individual rearing environment. This experimental method may provide a new way to analyze the behavior of mouse models of developmental disorders in a multiple-individual rearing environment, in which free-ranging behavior is possible.

Travellog – A Travel Buddy Finder Website

Travel Log: Redefining Group Travel" introduces a novel approach to exploration centered on socialconnection. Through curated experiences and spontaneous interactions, participants forge lasting friendships whilediscovering new cultures. Travel Log emerges as a platform that not only facilitates exploration but also cultivateslasting relationships beyond the journey's end. Social travel isn’t group travel (i.e. retirees trudging around after a tourguide with a plastic flag). It’s like-minded travellers around the world, getting together, expanding their socialnetworks, sharing experiences, pushing away from their comfort zones and becoming part of a global community.

Profiling the Interaction between Human Serum Albumin and Clinically Relevant HIV Reverse Transcriptase Inhibitors.

Tenofovir (TFV) is the active form of the prodrugs tenofovir disoproxil fumarate (TDF) and tenofovir alafenamide (TAF), both clinically prescribed as HIV reverse transcriptase inhibitors. The biophysical interactions between these compounds and human serum albumin (HSA), the primary carrier of exogenous compounds in the human bloodstream, have not yet been thoroughly characterized. Thus, the present study reports the interaction profile between HSA and TFV, TDF, and TAF via UV-Vis, steady-state, and time-resolved fluorescence techniques combined with isothermal titration calorimetry (ITC) and in silico calculations. A spontaneous interaction in the ground state, which does not perturb the microenvironment close to the Trp-214 residue, is classified as weak. In the case of HSA/TFV and HSA/TDF, the binding is both enthalpically and entropically driven, while for HSA/TAF, the binding is only entropically dominated. The binding constant (Ka) and thermodynamic parameters obtained via ITC assays agree with those obtained using steady-state fluorescence quenching measurements, reinforcing the reliability of the data. The small internal cavity known as site I is probably the main binding pocket for TFV due to the low steric volume of the drug. In contrast, most external sites (II and III) can better accommodate TAF due to the high steric volume of this prodrug. The cross-docking approach corroborated experimental drug-displacement assays, indicating that the binding affinity of TFV and TAF might be impacted by the presence of different compounds bound to albumin. Overall, the weak binding capacity of albumin to TFV, TDF, and TAF is one of the main factors for the low residence time of these antiretrovirals in the human bloodstream; however, positive cooperativity for TAF and TDF was detected in the presence of some drugs, which might improve their residence time (pharmacokinetic profile).

A spectrophotometric determination and the quantum chemical investigation of Pd(II)-3-hydroxy-2-(4-methoxyphenyl)-4-oxo-4H-1-benzopyran complex

An analytical approach has been instigated for spectrophotometric inquisition of trace amounts of palladium in its bivalent oxidation state employing a novel 1-benzopyran derivative viz. A 1:2 [Pd(II):HMPB] light yellow complex is formed by a spontaneous interaction between Pd(II) and HMPB. The complex is quantitatively extracted from a weakly basic medium into dichloromethane absorbing prevalently and consistently at 405-425 nm. The method coheres to linearity up to 1.5 µg mL-1 of palladium(II). Analytical parameters such as the molar absorption coefficient (ε=3.011 × 104 L mol-1 cm-1), Sandell's sensitivity [0.0035 µg Pd(II) cm-2], % RSD (0.62%) and the limit of detection [0.0147 µg Pd(II) mL-1] indicate that the study satisfies all the criteria for good sensitivity, precision and accuracy. In addition, intervention with regard to numerous anions/complexing agents and cations of primary analytical importance has been performed to assess the method's flexibility and usefulness. The results show that the majority of them do not cause any interference during determination. To better understand chemistry of the prepared complex, spectroscopic quantum chemical studies including DFT and MEP mapping based on examination of the electronic characteristics of the complex in its most stable least energy conformation are used.
 KEY WORDS: Palladium, 3-Hydroxy-2-(4-methoxyphenyl)-4-oxo-4H-1-benzopyran, Extractive spectrophotometric determination, DFT, MEP, Docking
 Bull. Chem. Soc. Ethiop. 2024, 38(3), 591-603. 
 DOI: https://dx.doi.org/10.4314/bcse.v38i3.4

Nucleotide‐Protocell Interactions: A Reciprocal Relationship in Prebiotically Pertinent Environments

AbstractSpontaneous interactions between nucleotides and lipid membranes are likely to have played a prominent role in the emergence of life on Earth. However, the effect of nucleotides on the physicochemical properties of model protocellular membranes is relatively less understood. To this end, we aimed to discern the effect of canonical nucleotides on the properties of single‐chain amphiphile membranes under prebiotically relevant conditions of multiple wet‐dry cycles. Furthermore, the change in the critical aggregation concentration of the membranes and their stability in the presence of nucleotides was also investigated in astrobiologically relevant analogue environments. We report that different nucleotides, lipid headgroups, and the ionic makeup of the system affect lipid‐nucleotide interactions, and these, in turn, modulate the effect of nucleotides on the membrane properties. Specifically, the presence of AMP, UMP, and CMP promoted self‐assembly of oleic acid membranes and increased their stability against certain prebiotically relevant selection pressures. This study takes us a step towards an appreciable understanding of how nucleotides might have shaped the protocellular landscape of the prebiotic Earth.

Religio-temporal framing and religio-social synchronization in family discourse in Saudi Arabia

Studies on time and timing in language often adopt psychological or cognitive perspectives. This work aims to study the relationship between time, religion, and family discourse. An interactional sociolinguistic approach was used to collect data from spontaneous family interactions to study how religious activities govern how family members in Saudi Arabia organize their day and simultaneously how different times of day are organized though religious activities. This study draws on Gordon’s study of family discourse, Van Leeuwen’s concepts of timing and Goffman’s concept of framing to investigate how family members organize their time in what can be referred to as religio-temporal framing and religio-social synchronization, and how this plays a role in the co-construction of Muslim identity.

Functional Connectivity of Language-Related Cerebellar Regions Is Reduced in Schizophrenia Patients.

Schizophrenia (SZ) is a widespread psychiatric disorder that is traditionally characterized by positive and negative symptoms. However, recent focus has shifted to cognitive deficits as a crucial aspect. The cerebellum, conventionally tied to motor coordination, is now recognized as pivotal in the pathophysiology of SZ cognitive impairments. Proposed disruptions in the cortico-cerebellar-thalamic-cortico circuit contribute to these deficits. Despite evidence of cerebellar abnormalities, within-cerebellum functional connectivity is often overlooked. This study explores spontaneous functional interactions within the cerebellum and their link to cognitive deficits in SZ. Using a multi-domain task battery (MDTB) parcellation, fMRI data from SZ patients and healthy controls were analyzed. Significant differences in cerebellar connectivity emerged, particularly in regions related to attention, language, and memory processing. Correlations between connectivity values and SZ symptomatology were identified. A post hoc analysis, considering the patients' hallucination vulnerability, revealed distinct connectivity patterns. Non-hallucinating and low-hallucinating SZ patients exhibited higher cerebellar connectivity than high-hallucinating patients, especially in language and motor control regions. These findings suggest a gradient of cerebellar connectivity alterations corresponding to hallucination vulnerability in SZ patients. This study offers novel insights into cerebellar impairments in SZ, highlighting the role of within-cerebellum connectivity in cognitive deficits. The observed connectivity patterns in language-related regions contribute to understanding language development and auditory verbal hallucinations in SZ.

Новые образы социальной реальности в цифровую эпоху

The process of increasing complexity of social reality must be seen as historically conditioned. The develop-ment of techniques and technologies that shape both human thinking and life is recognized as a major deter-minant of changing social reality. The main event of recent decades has certainly been digitalization, whose transformative impact on society and its institutions has been so great that a new term has appeared – the “digi-tal age”. Social reality in the digital age is being transformed by social media and virtual or augmented realities. The birth of digital reality from the spontaneous interaction of diverse realities has poorly predicted social and existential consequences. The real driver accelerating the formation of digital reality is the digitalization of the administrative and bureaucratic component of public administration. The most notable here are the provision of public services in digital format, the transfer of intradepartmental and interdepartmental interactions to electron-ic document management, a significant transformation in the nature and quality of feedback, and the manipu-lation of large amounts of data. In the process of digitalization, a reality is born that cannot be reduced to any kind of previously known realities. It is not described in ontological terms of virtuality and human existence manifests itself in it.

Moving-sensing-feeling bodies clamouring for contact in on-line therapy groups

ABSTRACT The author examines working with groups on-line from a Gestalt therapy perspective, an integrated therapeutic approach. The growing occurrence of the therapeutic group online setting brings the need to reconsider clinical tools to use bodily processes in the therapeutic relationship. This article provides a detailed and nuanced consideration of embodied process on-line and offers a practical and useful framework for clinicians, from a phenomenological, aesthetic and field oriented epistemological ground. In the on-line therapeutic group consideration needs to be given to the way embodied vitality can be engendered as supports for contact between members. Vitality is supported through a renewing of attention to the lived body as a support for spontaneous interactions, yet care is required in the on-line domain as there is a tendency towards disinhibition. The author explores how the relational grail of on-line therapy is an inter-embodied experience. This can be supported by the therapist cultivating an embodied presence, accompanied by an intentional disposition to wander with wonder as a way of surrendering to the unknowing of the virtual between. Such an orientation includes the therapist adopting an embodied hermeneutic phenomenological approach in group work that supports the co-creating of meaning between members in this trans-subjective situation.

Learning Through Teaching: How Physicians Learn Medicine in Authentic Clinical Contexts.

Little is known about the clinical knowledge and skills that are acquired by physicians through teaching, how such learning occurs, or the factors that influence this process. This study explored how physicians acquire clinical knowledge and skills through clinical teaching and examined the contextual elements that influence this learning. Two theoretical frameworks informed this interpretive description study: situated learning and cognitive apprenticeship. From March to November 2021, semistructured interviews and follow-up discussions were conducted at McGill University with clinician-teachers who regularly supervise internal medicine residents. Participants were asked to describe how they learned clinical medicine through spontaneous clinical teaching, guided by questions relating to what they learned, memorable teaching moments, and factors influencing this learning. Data were analyzed iteratively, using both a deductive and inductive approach. Of the 87 contacted physicians, 45 responded, expressing interest (n = 22) or declining participation (n = 23), and 42 did not respond. All 22 clinicians who responded positively were interviewed, with 7 follow-up discussions. Results suggested that clinician-teachers encountered myriad opportunities to learn clinical medicine during spontaneous interactions with trainees. These interactions, embedded in authentic patient care, were influenced by clinician-teacher characteristics, trainee characteristics, and contextual affordances. Clinician-teachers were stimulated to learn by trainee presence and through discrete interactions with trainees. These stimuli often led to feelings of "performative pressure" to role model and teach effectively or "slowing down" in thinking, prompting clinician-teachers to engage in learning processes (e.g., reflection, collaboration, and articulation), which resulted in knowledge acquisition, reinforcement, and refinement. Learning through teaching is an underappreciated strategy that can help clinician-teachers improve their clinical knowledge and skills. This study uncovered some of the processes through which clinicians learn during spontaneous clinical teaching and the factors that modulate this learning.

Programming temporal stiffness cues within extracellular matrix hydrogels for modelling cancer niches

Extracellular matrix (ECM) stiffening is a common occurrence during the progression of many diseases, such as breast cancer. To accurately mimic the pathophysiological context of disease within 3D in vitro models, there is high demand for smart biomaterials which replicate the dynamic and temporal mechanical cues of diseased states. This study describes a preclinical disease model, using breast cancer as an example, which replicates the dynamic plasticity of the tumour microenvironment by incorporating temporal (3-week progression) biomechanical cues within a tissue-specific hydrogel microenvironment. The composite hydrogel formulation, integrating adipose-derived decellularised ECM (AdECM) and silk fibroin, was initially crosslinked using a visible light-mediated system, and then progressively stiffened through spontaneous secondary structure interactions inherent between the polymer chains (∼10–15 kPa increase, with a final stiffness of 25 kPa). When encapsulated and cultured in vitro, MCF-7 breast cancer cells initially formed numerous, large spheroids (>1000 μm2 in area), however, with progressive temporal stiffening, cells demonstrated growth arrest and underwent phenotypic changes resulting in intratumoral heterogeneity. Unlike widely-investigated static mechanical models, this stiffening hydrogel allowed for progressive phenotypic changes to be observed, and fostered the development of mature organoid-like spheroids, which mimicked both the organisation and acinar-structures of mature breast epithelium. The spheroids contained a central population of cells which expressed aggressive cellular programs, evidenced by increased fibronectin expression and reduction of E-cadherin. The phenotypic heterogeneity observed using this model is more reflective of physiological tumours, demonstrating the importance of establishing temporal cues within preclinical models in future work. Overall, the developed model demonstrated a novel strategy to uncouple ECM biomechanical properties from the cellular complexities of the disease microenvironment and offers the potential for wide applicability in other 3D in vitro disease models through addition of tissue-specific dECM materials.

Signs of criticality in social explosions

The success of an on-line movement could be defined in terms of the shift to large-scale and the later off-line massive street actions of protests. The role of social media in this process is to facilitate the transformation from small or local feelings of disagreement into large-scale social actions. The way how social media achieves that effect is by growing clusters of people and groups with similar effervescent feelings, which otherwise would not be in touch with each other. It is natural to think that these kinds of macro social actions, as a consequence of the spontaneous and massive interactions, will attain the growth and divergence of those clusters, like the correlation length of statistical physics, giving rise to important simplifications on several statistics. In this work, we report the presence of signs of criticality in social demonstrations. Namely, similar power-law exponents are found whenever the distributions are calculated either considering time windows of the same length or with the same number of hashtag usages. In particular, the exponents for the distributions during the event were found to be smaller than before the event, and this is also observed either if we count the hashtags only once per user or if all their usages are considered. By means of network representations, we show that the systems present two kinds of high connectedness, characterised by either high or low values of modularity. The importance of analysing systems near a critical point is that any small disturbance can escalate and induce large-scale—nationwide—chain reactions.

The Impact on Peer Mentorship After Implementation of a Competency-Based Residency Curriculum in Canadian Radiation Oncology Training Programs

Peer mentorship provides professional and personal support between physicians with similar experiences and levels of training. While peer mentorship has shown to benefit academic success and professional growth, little data has examined contextual factors, such as curricular change, that may affect the quality of these relationships. This study aims to explore the impact of a new, nationwide radiation oncology (RO) residency curriculum, known as competence by design (CBD), on peer mentorship experiences between Canadian RO residents. A qualitative study, with a social constructivist approach, was conducted with 2 groups of Canadian RO residents. The first were those in the academic year before CBD implementation (non-CBD cohort), and the second were those in the inaugural year of CBD (CBD cohort). Semistructured 1-on-1 interviews were conducted to explore experiences of peer mentorship as it related to curriculum change. Interviews were transcribed and analyzed with deductive and inductive methods until data saturation. Between April and December 2021, 14 participants (6 non-CBD and 8 CBD residents) from 8 out of 10 eligible English-speaking RO training programs across Canada participated. Three major themes were identified: (1) the CBD cohort identified fewer opportunities for peer mentorship, with specific concerns regarding new evaluation processes and uncertainty about the later stages of training; (2) there was minimal impact on specialty-specific learning; and (3) peer mentorship thrived when occurring as spontaneous in-person interactions. Inaugural residents of a CBD curriculum perceived fewer opportunities for peer mentorship. There were specific concerns about new evaluative processes, though this did not affect specialty-specific learning. Peer mentorship was most impactful as informal and in-person interactions. Our findings suggest that unintended consequences of curriculum change may be mitigated by improving communication about new training objectives and increasing opportunities for informal interactions between residents.

Spontaneous bodily coordination varies across affective and intellectual child-adult interactions.

Research on child-adult interactions has identified that the morphology of bodily coordination seems to be sensitive to age and type of interaction. Mirror-like imitation emerges earlier in life and is more common during emotionally laden interactions, while anatomical imitation is acquired later and associated with cognitive tasks. However, it remains unclear whether these morphologies also vary with age and type of interaction during spontaneous coordination. Here we report a motion capture study comparing the spontaneous coordination patterns of thirty-five 3-year-old (20 girls; Mage = 3.15 years) and forty 6-year-old children (20 girls; Mage = 6.13 years) interacting with unacquainted adults during two storytelling sessions. The stories narrated the search of a character for her mother (Predominantly Affective Condition) or an object (Predominantly Intellectual Condition) inside a supermarket. Results show that children of both ages consistently coordinated their spontaneous movements towards adult storytellers, both in symmetric and asymmetric ways. However, symmetric coordination was more prominent in 3-year-old children and during predominantly emotional interactions, whereas asymmetric coordination prevailed in 6-year-old children and during predominantly intellectual interactions. These results add evidence from spontaneous interactions in favor of the hypothesis that symmetric coordination is associated with affective interactions and asymmetric coordination with intellectual ones.

Differential Effects of Aripiprazole on Electroencephalography-Recorded Gamma-Band Auditory Steady-State Response, Spontaneous Gamma Oscillations and Behavior in a Schizophrenia Rat Model.

The available antipsychotics for schizophrenia (SZ) only reduce positive symptoms and do not significantly modify SZ neurobiology. This has raised the question of the robustness and translational value of methods employed during drug development. Electroencephalography (EEG)-based measures like evoked and spontaneous gamma oscillations are considered robust translational biomarkers as they can be recorded in both patients and animal models to probe a key mechanism underlying all SZ symptoms: the excitation/inhibition imbalance mediated by N-methyl-D-aspartate receptor (NMDAr) hypofunction. Understanding the effects of commercialized atypical antipsychotics on such measures could therefore contribute to developing better therapies for SZ. Yet, the effects of such drugs on these EEG readouts are unknown. Here, we studied the effect of the atypical antipsychotic aripiprazole on the gamma-band auditory steady-state response (ASSR), spontaneous gamma oscillations and behavioral features in a SZ rat model induced by the NMDAr antagonist MK-801. Interestingly, we found that aripiprazole could not normalize MK-801-induced abnormalities in ASSR, spontaneous gamma oscillations or social interaction while it still improved MK-801-induced hyperactivity. Suggesting that aripiprazole is unable to normalize electrophysiological features underlying SZ symptoms, our results might explain aripiprazole's inefficacy towards the social interaction deficit in our model but also its limited efficacy against social symptoms in patients.

Optimum object selection methods for spontaneous gaze-based interaction with linear and circular trajectories

The demand for touchless technologies has increased since the outbreak of Covid-19. Spontaneous gaze-based interaction is a promising technique because it offers a natural approach for object selection using eye movements. There are two types of object trajectory in spontaneous gaze-based interaction: linear and circular trajectories. However, previous studies did not consider the optimum object selection method by taking into account the characteristics of each trajectory. In addition, previous studies suffered from high missed detection that caused spontaneous gaze-based applications to be less accurate and responsive. To address these scientific gaps, we conducted a novel study to propose optimum methods for object selection in linear and circular trajectories. The experimental results suggested that each trajectory requires a different combination of events detection and object selection techniques to achieve optimum performance. We recommend 2D Correlation as the object selection technique. In addition, we also recommend the Velocity and Movement Pattern Identification (I-VMP) and the Hidden Markov Model (HMM) as events detection techniques in linear and circular trajectories, respectively. The proposed approaches solved the missed detection problem and significantly increased the accuracy of gaze-based object selection to 95.60% and 99.73% on linear and circular trajectories, respectively. In the future, our discoveries are promising for the development of accurate, responsive, and seamless gaze-based touchless applications.

Antiproliferative activity of platinum(II) and copper(II) complexes containing novel biquinoxaline ligands.

Nowadays, cancer represents one of the major causes of death in humans worldwide, which renders the quest for new and improved antineoplastic agents to become an urgent issue in the field of biomedicine and human health. The present research focuses on the synthesis of 2,3,2',3'-tetra(pyridin-2-yl)-6,6'-biquinoxaline) and (2,3,2',3'-tetra(thiophen-2-yl)-6,6'-biquinoxaline) containing copper(II) and platinum(II) compounds as prodrug candidates. The binding interaction of these compounds with calf thymus DNA (CT-DNA) and human serum albumin were assessed with UV titration, thermal decomposition, viscometric, and fluorometric methods. The thermodynamical parameters and the temperature-dependent binding constant (K'b) values point out to spontaneous interactions between the complexes and CT-DNA via the van der Waals interactions and/or hydrogen bonding, except Cu(ttbq)Cl2 for which electrostatic interaction was proposed. The antitumor activity of the complexes against several human glioblastomata, lung, breast, cervix, and prostate cell lines were investigated by examining cell viability, oxidative stress, apoptosis-terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, in vitro migration and invasion, in vitro-comet DNA damage, and plasmid DNA interaction assays. The U87 and HeLa cells were investigated as the cancer cells most sensitive to our complexes. The exerted cytotoxic effect of complexes was attributed to the formation of the reactive oxygen species in vitro. It is clearly demonstrated that Cu(ttbq)Cl2, Pt(ttbq)Cl2, and Pt(tpbq)Cl2 have the highest DNA degradation potential and anticancer effect among the tested complexes by leading apoptosis. The wound healing and invasion analysis results also supported the higher anticancer activity of these two compounds.

What Happens to Psychological Safety When Going Remote?

Psychological safety is a precondition for learning and success in software teams. But what happens to psychological safety when work becomes remote? In this article, we explore how Norwegian software developers experienced remote work under the pandemic and after restrictions were waved and describe simple behaviors and attitudes related to psychological safety. We pay special attention to work arrangements in which team members alternate days in the office with days working remotely. Our key takeaway is that psychological safety is enabled by spontaneous interaction, which is easy to facilitate in the office and hard to facilitate remotely. Our findings lead us to recommend that team members align their work modes to increase chances for spontaneous interaction in the office while benefiting from the increased focus associated with working remotely.

Interactive mirrOring Games wIth sOCial rObot (IOGIOCO): a pilot study on the use of intransitive gestures in a sample of Italian preschool children with autism spectrum disorder.

Autism Spectrum Disorder (ASD) is a neurodevelopmental disorder characterized by persistent deficits in social communication, social interaction, and restricted behaviors. The importance of early intervention has been widely demonstrated, and developmental trajectories in ASD emphasize the importance of nonverbal communication, such as intransitive gesture production, as a possible positive prognostic factor for language development. The use of technological tools in the therapy of individuals with ASD has also become increasingly important due to their higher engagement and responsiveness to technological objects, such as robots. We developed a training protocol using the humanoid robot NAO, called IOGIOCO (Interactive mirroring Games wIth sOCial rObot), based on the use of intransitive gestures embedded in naturalistic dialogues, stimulating a triadic interaction between child, robot and therapist. The training was divided into six levels; the first 2 levels were called "familiarization levels," and the other 4 were "training levels". The technological setup includes different complexity levels, from mirroring tasks to building spontaneous interactions. We tested the protocol on 10 preschool children with ASD (aged 2-6 years) for 14 weeks. We assessed them at recruitment (T0), at the end of training (T1), and after 6 months (T2). We demonstrated the tolerability of the protocol. We found that one group (n=4, males and 2 females) reached the training level, while another and group (n=6 males) remained at a familiarization level (mirroring), we analyzed the results for the two groups. In the group that reached the training levels, we found promising results, such as an improvement in the Social Adaptive Domain of the ABAS-II questionnaire between T0 and T2. While current results will need a Randomized Controlled Trial to be confirmed, the present work sets an important milestone in using social robots for ASD treatment, aimed at impacting social and communication skills in everyday life.