Soybean Nutrition Research Articles

Nitrogen Fixation, Ureide, and Nitrate Accumulation Responses to Soybean Aphid Injury in Glycine max

ABSTRACT There is little information available about soybean aphid (Aphis glycines Matsumura) effects on the physiology and mineral nutrition of soybean (Glycine max [L.] merr.). Controlled-environment studies were conducted to measure soybean aphid infestation effects on dry weight, nitrogen (N) fixation, ureide-N, and nitrate-N concentration and accumulation. Plants grown in perlite using –N nutrient solution culture were infested at the 3rd trifoliolate (V3) stage and measured for N fixation, nodule characteristics, and ureide-N concentration at the full pod (R4) stage. When compared to uninfested control plants, aphid infestation reduced total nodule volume per plant by 34%, nodule leghemoglobin per plant by 31%, plant N fixation rate by 80% and shoot ureide-N concentration by 20%. Soil-grown plants were infested at the first trifoliolate (V1) stage and shoots were measured for dry weight, nitrate-N, and ureide-N at the full bloom (R2) stage. Infestation reduced shoot dry weight by 63%, increased nitrate-N concentration by 75%, but did not significantly affect ureide-N concentration. Because nutrient concentration is a single-point measurement that results from the integration of two dynamic processes, nutrient accumulation and dry matter production, we conclude that aphid-induced reductions in N fixation, coupled with decreased dry weight accumulation, caused shoot ureide-N concentration to remain unchanged in aphid-injured plants when compared to uninfested plants. Because nitrate-N concentration was greater in aphid-damaged shoot tissue, we further conclude that nitrate-N accumulation was less sensitive to aphid injury than dry weight accumulation.

Phosphorus nutrition on nitrogen metabolism of non-nodulated soybean

Two experiments were conducted under greenhouse conditions to study the influence of P deficiency on nitrogen nutrition of non-nodulated soybean. There was a reduction of N and P content in P deficient (P-) plants of almost 5... | Find, read and cite all the research you need on Tech Science Press

Aplicação superficial de escória, lama cal, lodos de esgoto e calcário na cultura da soja

O objetivo deste trabalho foi avaliar o efeito da aplicação superficial de lodos de esgoto, lama cal, escória de aciaria e calcário sobre o estado nutricional e a produtividade da soja, em sistema plantio direto. O delineamento foi o de blocos ao acaso em arranjo fatorial 4x4+1, constituído por quatro tratamentos - resíduos de lodo de esgoto centrifugado (LC) e de biodigestor (LB), escória de aciaria (E) e lama cal (Lcal) - nas doses 0, 2, 4 e 8 Mg ha-1, mais o controle com 2 Mg ha-1 de calcário. As plantas de soja apresentaram maior concentração de nitrogênio, fósforo e cálcio, em 2003, 2004 e 2005, e de potássio, em 2003 e 2004, em razão dos tratamentos LC, LB, E, Lcal e calagem. A produtividade da soja foi favorecida pela aplicação dos tratamentos no sistema plantio direto, em 2003, 2004 e 2005. O fósforo, e o cálcio contribuíram para o aumento da produtividade da soja em 2003 e 2004.

RELAÇÕES ENTRE DOSES DE CALCÁRIO E MANGANÊS NA NUTRIÇÃO MINERAL DA SOJA NA REGIÃO DE RIO VERDE-GO

This study aimed to assess the effects of liming and manganese rates on mineral nutrition of soybean on two representative soils from Rio Verde-GO, Brazil. A greenhouse experiment was undertaken at the School of Agronomy (Federal University of Goias, Brazil), using an Oxisol (Latossolo Vermelho Amarelo Distrofico fase Cerrado) and an Ultipsamment (Neossolo Quartzarenico Distrofico fase Cerrado). A completely randomized experimental design was used, in a 5x5 factorial treatment design (liming and manganese rates), with three replications. The studied soils showed serious nutritional shortcomings, due to their high acidity levels and excess of exchangeable manganese. Treatments without liming provoked widespread Ca and Mg deficiencies and manganese toxicity, with visual symptoms in soybean leaves. Close relations among Ca, Mg, and Mn accumulated in soybean leaves as functions of applied liming and manganese rates have been found. One may conclude that liming is essential both as a soil acidity neutralizer, as a nutritional source of Ca and, as well to prevent accumulation of manganese in plants at toxic levels. Application of Mn on both studied soils is unnecessary for the soybean crop. KEY-WORDS: Cerrados; Oxisols; Ultipsamment; soybeans; liming; manganese.

Nutrient concentration in soil water extracts and soybean nutrition in response to lime and gypsum applications to an acid Oxisol under no-till system

No-till system (NTS) occupies 20 million hectares with grain crops in Brazil. However, calcium deficiency and aluminum toxicity can limit crop yields in many soils, and liming, associated to gypsum application, is an option for improving soil management. The objective of this study was to evaluate the effects of lime and gypsum application on the composition of soil water extracts of a clayey Rhodic Hapludox, cultivated with soybean under NTS. The experiment had a randomized complete block design with split-plots. The plots consisted of lime treatments (either a single rate of 4.5 t ha−1 or three annual rates of 1.5 t ha−1) surface-applied or incorporated at 0.2 m depth. The subplots received surface applications of gypsum (3, 6 and 9 t ha−1). Liming increased total calcium and magnesium concentrations and the magnesium free Form activity (aMg2+) in the water extracts. The effect of liming on Mg was observed at deeper layers of the soil profile. Gypsum increased total concentration and free forms activities at calcium (aCa2+) and sulfate, but decreased to magnesium in the 0.05–0.2 m soil layer. Part of Mg lost from these upper layers probably contributed to increased Mg in the subsoil (0.4–0.8 m). Free forms activities at the aluminum, calcium, magnesium and sulfate were lower than the total concentrations, mainly for aluminum. Ca and Mg concentrations in soybean leaf tissue were positively correlated to the aCa2+ and aMg2+ in the soil water extract. Soybean grain yield was negatively correlated to both Mn total concentration and activity (free form) in the soil water extract, but it was positively correlated to sulfate (total concentration and free form activity) in the subsoil layer and to the Ca total concentration in the upper layer (0–0.05 m). It is concluded that lime and gypsum ameliorate soybean grain yield under NTS.

Aplicação simultânea de dessecantes e boro no manejo de plantas daninhas e na nutrição mineral das culturas de soja e girassol

Dois experimentos foram conduzidos com o objetivo de avaliar o controle de plantas daninhas em pré-semeadura da soja (Glycine max) e do girassol (Helianthus annuus), por meio de aplicações de herbicidas dessecantes, isolados ou em combinação com boro, bem como a resposta dessas culturas à aplicação desse micronutriente. Nas parcelas de soja, foram aplicados os tratamentos glyphosate (1,44 kg e.a. ha-1), glyphosate potássico (2,48 kg i.a. ha-1), diuron (0,2 kg i.a. ha-1) + paraquat (0,4 kg i.a. ha-1), e paraquat (0,400 kg i.a. ha-1). Nas parcelas de girassol, foram aplicados os tratamentos glyphosate (0,54 kg e.a. ha-1), glyphosate (0,72 kg e.a. ha-1), glyphosate potássico (1,24 kg i.a. ha-1), paraquat (0,4 kg i.a. ha-1), glyphosate (0,72 kg e.a. ha-1) + flumioxazin (0,025 kg i.a. ha-1) e glyphosate (0,72 kg e.a. ha-1) + carfentrazone (0,02 kg i.a. ha-1). Ambos os experimentos continham as testemunhas capinada e sem capina. As subparcelas dos dois experimentos foram constituídas pela ausência ou presença de B, junto à calda de pulverização, na fonte ácido bórico [H3BO3 - 17% B]. A adição de ácido bórico à calda de pulverização não prejudicou o controle das plantas daninhas pelos tratamentos dessecantes, exceto para a mistura formulada de paraquat + diuron. Houve aumento dos teores de boro no solo e nas folhas da cultura da soja e do girassol quando foram associados os tratamentos com herbicidas dessecantes e o ácido bórico. É viável a aplicação de herbicidas dessecantes e ácido bórico, controlando as plantas daninhas em présemeadura e aumentando o teor de B no solo e nas plantas de soja e de girassol.

Nitrogen nutrition of soybean in Brazil: Contributions of biological N2 fixation and N fertilizer to grain yield

There has recently been concern in Brazil whether biological N2 fixation (BNF) is capable of meeting the increased N needs of newly released more productive cultivars, as well as doubts about the advantages of annual reinoculation of seeds. Forty experiments were performed over 3 yr in oxisols containing at least 103 cells of Bradyrhizobium g-1 in the State of Paraná, southern Brazil to estimate the contributions of BNF and of N fertilizer. The experiments were performed at two sites, Londrina and Ponta Grossa, under conventional (CT) or no-tillage (NT) systems, with two cultivars [Embrapa 48 (early-maturing) or BRS 134 (medium-maturity group)]. Treatments included non-inoculated controls without or with 200 kg of N ha-1, and inoculation without or with N fertilizer applied at sowing (30 kg of N ha-1), or at the R2 or R4 stage (50 kg of N ha-1). Compared with the non-inoculated control, reinoculation significantly increased the contribution of BNF estimated by the N-ureide technique (on average from 79 to 84%), grain yield (on average 127 kg ha-1, or 4.7%) and total N in grains (on average 6.6%). The application of 200 kg of N fertilizer ha-1 drastically decreased nodulation and the contribution of BNF (to 44%), with no further gains in yield. Application of starter N at sowing decreased nodulation and the contribution of BNF slightly and did not increase yields, while N fertilizer at R2 and R4 stages decreased the contribution of BNF (to 77%) and also yields. Estimates of volatilization of ammonia ranged from 15 to 25% of the N fertilizer applied, and no residual benefits of the N fertilizer in the winter crop were observed. The results highlight the economical and environmental benefits resulting from replacing N fertilizer with inoculation in Brazil, and reinforce the benefits of reinoculation, even in soils with high populations of Bradyrhizobium. Key words: Biological nitrogen fixation, Bradyrhizobium, inoculation, N fertilizers, ureides, soybean

Formulation of Environmentally Sound Waste Mixtures for Land Application

Major impediments to the land application of coal combustion byproducts (fly ash) for crop fertilization have been the presence of heavy metals and their relatively low and imbalanced essential nutrient concentration. Although nutrient deficiencies, in particular N, P, and K, may be readily augmented by adding organic wastes such as sewage sludge and animal manure, the indiscriminate application of mixtures to crops can cause excessive soil alkalinity, imbalanced nutrition (P, Mg), phytotoxicities (B, Mn, ammonia, nitrite), and unspecified contamination of the food chain by elements such as As. In this study, nutrient availability data and linear programming (LP) were used to solve these problems by formulating fly ash-biosolid triple mixtures which complied with both plant and soil fertilization requirements, and met existing U.S.A. environmental regulations for total As application in sewage sludge (EPA-503). Thirteen different fly ash samples were LP-formulated with sewage sludge, poultry manure, CaCO3, and KCl to yield 13 unique mixtures, which were then evaluated in greenhouse pot experiments. Results indicated that normal growth and balanced nutrition of sorghum (Sorghumbicolor L.) and soybean (Glycine max (L.) Merr.) crops were achieved in all mixtures, comparable to a balanced fertilizer reference treatment, and significantly better than the untreated control. Phytotoxic levels of B, NH3, NO2 -, overliming problems, and excessive As levels which were previously encountered from indiscriminate use of these waste materials, were all well controlled by LP-formulated mixtures. Most fly ash quantities in mixtures were limited by either available B (< 4 kg ha-1) or total As (< 2 kg ha-1) restrictions during formulation, while the most alkaline fly ash was limited by its high calcium carbonate equivalence (CCE = 53.9%). These results confirmed that fly ash land application should not be at arbitrary fixed rates, but should be variable, depending on the soil, crop, and particularly the fly ash chemistry.

Crescimento radicular e nutrição da soja cultivada no sistema plantio direto em resposta ao calcário e gesso na superfície

A deficiência de cálcio e a toxidez de alumínio têm sido os fatores de acidez mais limitantes ao crescimento de raízes. Com o objetivo de avaliar o crescimento radicular e a absorção de nutrientes pela soja, cv. EMBRAPA 58, e seus reflexos sobre a produção de grãos, considerando as doses de calcário e gesso na superfície, em sistema plantio direto, foi realizado um experimento em um Latossolo Vermelho distrófico textura média, em Ponta Grossa (PR). O delineamento experimental empregado foi o de blocos ao acaso em parcela subdividida, com três repetições. Foram utilizadas quatro doses de calcário dolomítico, com 84% de PRNT: 0, 2, 4 e 6 t ha-1, e quatro doses de gesso agrícola: 0, 4, 8 e 12 t ha-1. A calagem foi realizada em julho e a aplicação de gesso em novembro de 1993. O crescimento radicular da soja não foi afetado pelas condições de acidez, em solo com 15, 12 e 8 mmol c dm-3 de Ca trocável e 28, 32 e 40% de saturação por Al, respectivamente, nas profundidades de 0-10, 10-20 e 20-40 cm. Houve aumento da absorção, pela cultura da soja, de todos os macronutrientes com a calagem, e de Ca e S com a aplicação de gesso. Somente a calagem aumentou a produção de soja, em decorrência de maior disponibilidade e absorção de Mg e de redução dos teores foliares de Zn e Mn.

Fungos micorrízicos e fósforo, no crescimento, nos teores de nutrientes e na produção do sorgo e soja consorciados

O objetivo deste trabalho foi avaliar em casa de vegetação os efeitos da inoculação de fungos micorrízicos arbusculares sobre o crescimento, produção de grãos e teores de nutrientes no sorgo, e na soja, consorciados, num Latossolo Vermelho-Escuro fumigado que recebeu 0, 25, 50, 100 e 200 mg/kg de P. O peso da matéria seca da parte aérea do sorgo aumentou em 55%, 33% e 6%, respectivamente, com o Glomus etunicatum, o Gigaspora margarita e o Glomus clarum. O peso dos grãos secos aumentou em 31% e 21%, respectivamente, com o Glomus etunicatum e com o Gigaspora margarita. Na soja, o peso da matéria seca da parte aérea aumentou linearmente com as doses de P, independentemente da inoculação de fungo micorrízico, enquanto o peso dos grãos secos aumentou na mesma grandeza que o do sorgo. O Glomus etunicatum foi a espécie micorrízica mais eficiente em todas as doses de P. A inoculação de fungos micorrízicos contribuiu para o aumento da matéria seca, produção de grãos e para os teores dos nutrientes N, P, K, Zn e Cu no sorgo e na soja. Estes efeitos são dependentes das doses de P no solo e da espécie de fungos micorrízicos inoculados.

Production of aerobactin by microorganisms from a compost enrichment culture and soybean utilization

Fe chelators produced by compost microorganisms in C enrichment culture can efficiently improve Fe nutrition of soybean and oat under slightly alkaline conditions. Little information is available, however, concerning the identity of these chelators. A study was conducted to identify the Fe chelators and the microorganisms responsible for their production and to evaluate the mechanism of Fe uptake by soybean of these Fe chelates. One Fe chelator predominated in the culture solution and, after purification using chromatography, it was identified by nuclear magnetic resonance and mass spectrometry as the siderophore, aerobactin. A strain of Citrobacter diversus, which produced a high concentration of aerobactin, was isolated and purified from the culture. Bathophenanthrolinedisulfonic acid (BPDS), an Fe2+ trap, did not decrease soybean uptake of Fe of which more than 90% was in the form of an Fe‐aerobactin complex. Reduction of Fe as Fe‐aerobactin by soybean roots was not detected. Iron uptake was, however, significantly (P∼ 0.05) inhibited by NaN3, a respiratory inhibitor. These results suggest that the Fe may have been taken up by soybean as an Fe‐aerobactin complex through an active transport mechanism.

Nitrogen fixation capacity and nodule occupancy by Bradyrhizobium japonicum and B. elkanii strains

In a previous study soybean Bradyrhizobium strains, used in Brazilian studies and inoculants over the last 30 years, and strains adapted to the Brazilian Cerrados, a region frequently submitted to environmental and nutritional stresses, were analyzed for 32 morphological and physiological parameters in vivo and in vitro. A cluster analysis allowed the subdivision of these strains into species Bradyrhizobium japonicum, Bradyrhizobium elkanii and a mixed genotype. In this study, the bacteria were analyzed for nodulation, N2 fixation capacity, nodule occupancy and the ability to increase yield. The goal was to find a relationship between the strain groups and the symbiotic performance. Two strains of Brazilian B. japonicum showed higher rates of N2 fixation and nodule efficiency (mg of N mg–1 of nodules) under axenic conditions. These strains also showed greater yield increases in field experiments when compared to B. elkanii strains. However, no differences were detected between B. japonicum and B. elkanii strains when comparing nodule occupancy capacity. The adapted strains belonging to the serogroup B. elkanii SEMIA 566, most clustered in a mixed genotype, were more competitive than the parental strain, and some showed a higher capacity of N2 fixation. Some of the adapted strains, such as S-370 and S-372, have shown similar N2 fixation rates and nodulation competitiveness to two Brazilian strains of B. japonicum. This similarity demonstrates the possibility of enhancing N2 fixing ability, after local adaptation, even within B. elkanii species. Differences in the DNA profiles were also detected between the parental SEMIA 566 and the adapted strains by analyses with the ERIC and REP-PCR techniques. Consequently, genetic, morphological and physiological changes can be a result of adaptation of rhizobia to the soil. This variability can be used to select strains capable of increasing the contribution of N2 fixation to soybean nutrition.

Protein, Fiber, and Lipid Content of Vegetable Soybean

There has been an increased interest in soybean nutrition. Soybean is harvested for oil and soyfood production, but may also be for use as a vegetable. Matured soybean and field-type soybean, from which soyfood and oil are manufactured, are high in protein and linoleic acid, respectively. Little is known about these nutrient contents in vegetable soybean. We analyzed the vegetable soybean's protein, fiber, and fatty acid composition. Thirty-one vegetable soybean genotypes from Maturity Groups III-VI harvested between Growth Stages 6 and 7 were shelled and freeze dried. Kjeldahl and Weende methods were used to analyze nitrogen and crude fiber, respectively. After extraction by Maxwell's method, gas chromatography was used to analyze total lipid and fatty acids. Protein was calculated by multiplying nitrogen by 6.25. Mean grams % of dry vegetable soybean were: 7.5 crude fiber; 36.9 protein; and 18.3 total lipid. Mean fatty acids % of total lipid were: 19.08 palmitoleic acid; 15.80 oleic acid; 44.77 linoleic acid; and 12.94 linolenic acid. There were significant correlations (p>0.01) among these nutrients. All nutrients showed significant differences among the genotypes, indicating high variabilities. When selecting genotypes for production, attention should be given to the nutrient content of the genotypes to breed a vegetable soybean having enhanced oleic acid and fiber content.

Soybean iron metabolism and nutrition

Soybean iron metabolism and nutrition

Fe chelates from compost microorganisms improve Fe nutrition of soybean and oat

Iron availability to plants is often limited when soil pH is 7 or higher. In C rich, but Fe limiting environments, microorganisms may produce organic chelators that complex Fe and increase its availability to plants. Seedlings of soybean (Glycine max L.) and oat (Avena sativa L.) plants, with Fe-efficient or inefficient uptake mechanisms, were grown in an Fe free nutrient solution at pH 7.5. Experiments (using a complete factorial design) were conducted in which these seedlings were transferred to a fresh nutrient solution and treated with Fe sources (FeCl3, FeEDDHA, and Fe complexed with chelators produced by compost microorganisms (CCMs) after their enrichment in an Fe free, glucose medium), Fe concentrations (0 and 6.7 μM) and antibiotic (0 and 100 mg streptomycin L-1). Dry weight of soybean plants and Fe uptake were significantly (P ≤ 0.05) higher when Fe was supplied as 59FeCCM than as59 FeCl3 and similar to when Fe was supplied as59 FeEDDHA. Dry weight of the Fe-inefficient Tam 0-312 oat cultivar was also significantly higher when Fe was supplied as FeCCM. Fe uptake by oat, when supplied as 59FeCCM, was twice that for59 FeEDDHA and 59FeCl3. Chlorophyll concentration in plants supplied with FeCCM and FeEDDHA was significantly greater (P ≤ 0.05) than in minus Fe control plants and in FeCl3 supplied plants. Activities of catalase and peroxidase, measured as indicators of Fe nutrition in soybean and oats, were generally increased when Fe was supplied with FeCCM as compared to the other Fe sources. The experimental conditions in which the CCMs were produced are similar to those in soil after amendment with manures or other readily available organic materials. These CCMs can bind with Fe, even under slightly alkaline conditions, and effectively improve Fe nutrition of soybean and oat.

Characteristics of Growth and N Nutrition of Soybean Affected by Cool-Summer Damage in 1993 Estimated by Rb/K Ratio Method and Relative Ureide Method

Characteristics of Growth and N Nutrition of Soybean Affected by Cool-Summer Damage in 1993 Estimated by Rb/K Ratio Method and Relative Ureide Method

Effect of split application of potassium on growth, yield and potassium accumulation by soybean

Field experiments were conducted during 1989 to 1991 on a loamy sand (Typic ustochrept) soil to study the K nutrition of soybean. Soybean responded significantly up to 50 kg K ha−1 when applied 1/2 of K at planting and 1/2 at flower initiation (two split) or applying 1/3 K at planting, 1/3 at flower initiation and 1/3 at pod development (three splits). Split application was more beneficial than applying full K at time of planting due to higher leaf area index, crop growth rate, chlorophyll content of fresh leaves, K accumulation in soybean and better agronomic and physiological efficiency of applied K. Agronomic efficiency, physiological efficiency and apparent recovery of K reduced as rate of applied K was increased from 50 to 75 kg ha−1. Highest K+ concentration (3.4 % of dry matter) was recorded in 30-day-old plants at 75 kg K ha−1 which depressed progressively with the age of the crop. At maturity, the K concentration of soybean seed varied from 1.5% (unfertilised K plants) to 2.1 % (when 75 kg K ha−1 was applied in three splits).

Potassium fertilization, root morphology and potassium absorption by soybean

Abstract A greenhouse experiment studied the effect of potassium fertilization on soybean (Glycine max L. Merrill) root morphology and on ? absorption by six soybean cultivars of different maturation groups and growth habits. The plants were grown up to 70 days after plant emergence, in pots containing 6.0 kg of soil. In the absence of K, no significant difference in ? absorption was observed among the cultivars or in root length and surface, but root mean radius was correlated to ? absorption. Differences in ? absorption were not associated with root characteristics in the presence of ? fertilization. Physiological adjustments in ? uptake, as well as ? availability in the soil, were more important in soybean nutrition than were morphological adjustments in the root system. The results were not associated with plant growth habit or with maturation group.

Fertilization and mineral nutrition of soybean in mesa de Guanipa, Anzoategui State, Venezuela

Abstract An experiment was conducted on some soils of Mesa de Guanipa, which are sandy, acid (pH 5.5), and have low levels of available P, K, Ca, and Mg. These soils are located at the Agropecuaria Guanipa farm in El Tigre, Anzoategui State, Venezuela. The main objective was to evaluate rates and sources of P, and rates of K on soybean yield (variety FP‐3), which is an activity of the project “Maximum soybean yields in Venezuela”;, financed by the Potash and Phosphate Institute of Canada. The rates and sources of P were 0, 75, and 150 kg P2O5/ha as triple superphosphate (TSP) and diammonium phosphate (DAP). Rates of potassium as K2O were 0, 60, and 120 kg/ha as potassium chloride (KCl). The experimental design was a randomized complete block with 3 replications and a final plant density equivalent to 400,000 plants/ha. A basic application of nitrogen and magnesium fertilizers was made to guarantee the supply of those nutrients. The soybean seeds were inoculated at planting with NITROBAC to ensure nodule f...

Vanadium: Toxicity and accumulation by beans

Hydroponic and rhizotron studies were conducted to determine the effect of V on soybean [Glycine max (L.) Merr.] and bush bean (Phaseolus vulgaris L.) biomass and mineral nutrition. In the hydroponic study, 6 mg V L−1 (as VOSO4) in the nutrient solution drastically altered soybean nutrition, and significantly reduced plant biomass. Vanadium accumulated in the roots but not in the aerial portions of the plants. The data support the hypothesis that tissue V and Ca contents are related with high concentrations of both elements in the roots, and low concentrations in the aerial portions of the plant. Analysis of data with the Diagnostic and Recommendation Integrated System (DRIS) identified Ca as deficient in aerial tissues. The changes in Ca concentrations induced by V treatment may also have antagonized the concentrations of others macronutrients, most notably K and Mg. DRIS also indicated that K, Mg, and Zn levels were relatively high. The rhizotron study, which dealt with bush beans grown in metal-treated soils, further showed that V was primarily concentrated in the roots of the plants, with very little accumulated in the aerial portions.