Soybean Glycine Max Research Articles

The Effect of Learning Biophysics with STEM Approach on Science Process Skills and Critical Thinking: Field Study on Application of NA-AOGS for Increasing Soybean Productivity and Growth Rate

Efforts to fulfill food security to anticipate population growth in Indonesia need to be supported by universities through research and community service. The application of the Natural Animal Audio Organic Growth System (NA-AOGS) with a STEM (Science, Technology, Engineering, and Mathematics) approach is an effort to help the community increase crop yields and accelerate the growth of soybeans (Glycine max (L.) Merill). The research method used is R&D (Research & Development) which is modified from Cennamo and Kalk (2018). This model is in the form of a spiral cycle using 5 (five) main activities as follows: (1) defining the product conceptual; (2) designing research products; (3) demonstrating the product in a limited trial; (4) developing the product through the process of evaluating the test results, and (5) presenting the product to the public. The direct impact of learning is the improvement of science process skills and critical thinking of students participating in the Biophysics course at the Natural Sciences Study Program, Universitas Negeri Yogyakarta. This is due to the effective application of STEM to link science learning with contextual technology and engineering (NA-AOGS), environmentally friendly technology (only using natural animal sound frequency variables), mathematical elements in data analysis, and analysis of soybeans plant growth charts. The economic impact of this research is the scientific contribution in the form of adaptation of agricultural technology so that it can increase soybean yields (an increase of 130.38% on an area of 2750 m2). The novelty and contribution of this research is the integration of science learning with agricultural techniques and technology with a STEM approach so that it can improve the welfare of farmers in educational institutions as a new model in science learning.

Quality Evaluation of Butter Cake Prepared by Substitution of Wheat Flour with Green Soybean (Glycine Max L.) Okara

ABSTRACT Using byproducts from the food industry to produce healthy and sustainable food was an integral component of the sustainable development goals. This research was aimed to assess the effect of substituting wheat flour with nutrient-rich green soybean okara (GSBO) flour on the quality and nutritional values of butter cake. The result showed that the GSBO-substituted flour had significantly (p ≤ 0.05) higher water and oil absorption capacities, higher protein, fiber and total phenolic content (TPC), and more favorable sensory characteristics compared to wheat flour. Specifically, 20% GSBO butter cake contained significantly (p ≤ 0.05) highest amounts of protein, fiber, TPC, and scores of all sensory characteristics. In conclusion, substitution of up to 20% GSBO to wheat flour could improve the nutritional quality of butter cake. Application of GSBO flour in butter cake contributes to the sustainable goal of achieving zero waste and development of a plant protein-based product in sustainable healthy diet.

Effects of Sargassum crassifolium Extract Formula as Biostimulant on Growth and Yield of Glycine max L. Merill

The research about the Effect of Sargassum crassifolium Extract Formula as Biostimulant to Increase Growth and Yield of Glycine max L. Merill was conducted from February until June 2020 at the warehouse of Plant Physiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University, Padang. The research aims to evaluate the effectiveness of liquid and powder extract formula of S. crassifolium as biostimulant on the growth and yield of soybean (Glycine max L. Merill). The research was conducted by an experimental method arrange in a Completely Randomized Design (CRD). The experiment consisted of 3 treatments. Each treatment was laid out with nine replications. The treatments tested were control, liquid extract (0.4%), powder extract (1.6 g/L). The results showed that the application of liquid extract gave a higher tendency towards the improvement of the root system, fresh and dry weight, chlorophyll content, and 100 seeds weight of soybean plant. While the powder extracts shown the best result on the total seed weight. Both of extract formula had the potential to be used to increase growth and yield of soybean.

Genome-Wide Association Study to Map Genomic Regions Related to the Initiation Time of Four Growth Stage Traits in Soybean.

The time to flowering (DF), pod beginning (DPB), seed formation (DSF), and maturity initiation (DMI) in soybean (Glycine max [L.] Merr) are important characteristics of growth stage traits (GSTs) in Chinese summer-sowing soybean, and are influenced by genetic as well as environmental factors. To better understand the molecular mechanism underlying the initiation times of GSTs, we investigated four GSTs of 309 diverse soybean accessions in six different environments and Best Linear Unbiased Prediction values. Furthermore, the genome-wide association study was conducted by a Fixed and random model Circulating Probability Unification method using over 60,000 single nucleotide polymorphism (SNP) markers to identify the significant quantitative trait nucleotide (QTN) regions with phenotypic data. As a result, 212 SNPs within 102 QTN regions were associated with four GSTs. Of which, eight stable regions were repeatedly detected in least three datasets for one GST. Interestingly, half of the QTN regions overlapped with previously reported quantitative trait loci or well-known soybean growth period genes. The hotspots associated with all GSTs were concentrated on chromosome 10. E2 (Glyma10g36600), a gene with a known function in regulating flowering and maturity in soybean, is also found on this chromosome. Thus, this genomic region may account for the strong correlation among the four GSTs. All the significant SNPs in the remaining 7 QTN regions could cause the significant phenotypic variation with both the major and minor alleles. Two hundred and seventy-five genes in soybean and their homologs in Arabidopsis were screened within ± 500 kb of 7 peak SNPs in the corresponding QTN regions. Most of the genes are involved in flowering, response to auxin stimulus, or regulation of seed germination, among others. The findings reported here provide an insight for genetic improvement which will aid in breeding of soybean cultivars that can be adapted to the various summer sowing areas in China and beyond.

Response of Growth and Yield of Soybean (Glycine max l. Merril) to the Method and Dose of Leachate Liquid Organic Fertilizer Application

The high demand for soybeans cannot yet be met by domestic production. Thus, a way to increase soybean production is necessary, one of which is to use liquid organic fertilizer derived from landfill leachate. This study aimed to examine the application method of liquid organic fertilizer (LOF) from landfill leachate to the soil and leaves and to determine the concentration giving the best growth and yield of soybean plants. The study was a field-research arranged in a completely randomized design (CRD) consisting of six treatments and four replications, which was carried out in a greenhouse. The treatments include A (0 LOF/L water), B (20 ml LOF/L water), C (30 ml LOF/L water, D (40 ml LOF/L water), E (50 ml LOF/L water) and F (60 ml LOF/L water). The liquid organic fertilizer was applied through the soil and leaves at a dose according to the treatments. The results showed that the application of liquid organic fertilizer from landfill leachate was better applied to the soil than to the leaves. The liquid organic fertilizer given had a significant effect on the leaf area (cm2), crop weight (g), and plant seed weight (g), but not on the plant height (cm) and stem diameter (cm). Liquid organic fertilizer application is better given to the soil than to the leaves based on the independent T test. The liquid organic fertilizer from landfill leachate at a concentration of 40 ml/L of water had the best effect on the growth and yield of soybean plants.

Effect of water hyacinth compost on the morpho-physiological parameters of Soybean (Glycine max l.)

A pot experiment was conducted under rainfed condition to study the effect of water hyacinth compost on the morpho-physiological parameters of soybean (Glycine max L.) at the Teaching and Research Farm of Faculty of Agriculture and Veterinary Medicine, Imo State University, Owerri. The treatments were control (T1) 100g (T2), 150g (T3) and 200g (T4) of water hyacinth compost and replicated four times. The treatments were arranged in Complete Randomized Design (CRD). The parameters measured were plant height, number of leaves, leaf area (cm2), leaf area index, relative growth rate (RGR), Net assimilation rate (NAR), shoot dry weight(g), yield and yield components (Number of pods, pods weight, 100 seed weight). The results obtained indicated that T3 significantly produced highest plant height (57.6cm) compare to control. While it was observed that T4 (200g) significantly produced the highest number of leaves (233.25), leaf area (631.80cm2), shoot dry weight (15.445g), number of pods (129.75), pod weights (25.38g) seed weight (7.23g) and yield (0.72kg/ha) relative to control and other treatment levels. Root parameters were also significantly improved by the rates of water hyacinth application compared to control. It will be worthy to note that there was no nodulation perhaps that was why the yield was poor. The results showed that soybean growth can effectively be improved with incorporation of water hyacinth into soil.

Effect of Soy Leaf Flavonoids on Pea Aphid Probing Behavior.

Simple SummaryFlavonoids are plant phenolic compounds whose biological activities include participation in plant responses to various stresses of biological and environmental origins, including protection against insect herbivore attack. We were interested in whether the specific flavonoids detected in soybean leaves have the potential to discourage the pea aphid from infesting other leguminous plants, peas in particular. We immersed the pea leaves in ethanolic solutions of the flavonoids apigenin, daidzein, genistein and kaempferol, offered them to the pea aphids and observed their behavior when they probed plant tissues with their piercing–sucking mouthparts. We discovered that aphids readily probed the pea leaves whether they were treated with flavonoids or not. However, later on, the behavior of the aphids changed depending on the flavonoid applied. Apigenin, daidzein and kaempferol caused a decrease in the intensity of plant sap ingestion. In addition, daidzein and kaempferol made the finding of sap-transporting vessels more difficult for aphids. In contrast, genistein did not affect the pea aphids’ feeding activity. Our findings provide the plant breeders and plant protection services with information on what direction their efforts should take to protect leguminous plants against aphids in a sustainable and environmentally friendly way.Flavonoids detected in soybean Glycine max (L.) Merr. (Fabaceae) cause various alterations in the metabolism, behavior, and development of insect herbivores. The pea aphid Acyrthosiphon pisum (Harris) (Hemiptera: Aphididae) poses potential threat to soybeans, but the effect of individual flavonoids on its feeding-associated behavior is relatively unknown. We monitored probing behavior (stylet penetration activities) of A. pisum on its preferred host plant, Pisum sativum L. untreated (control) and treated with 0.1% ethanolic solutions of flavonoids apigenin, daidzein, genistein, and kaempferol. We applied the electrical penetration graph (electropenetrography, EPG) technique, which visualizes the movements of aphid stylets within plant tissues. None of the applied flavonoids affected the propensity to probe the plants by A. pisum. However, apigenin enhanced the duration of probes in non-phloem tissues, which caused an increase in the frequency and duration of stylet mechanics derailment and xylem sap ingestion but limited the ingestion of phloem sap. Daidzein caused a delay in reaching phloem vessels and limited sap ingestion. Kaempferol caused a reduction in the frequency and duration of the phloem phase. Genistein did not affect aphid probing behavior. Our findings provide information for selective breeding programs of resistant plant cultivars to A. pisum.

Effect of Phosphorous Application on Yield and its Uptake by Soybean (Glycine max L.) in Different Cropping Systems

Background: Soybean-wheat is the most dominant soybean based cropping system and it also fits well in soybean-spring maize and soybean-gobhi sarson cropping systems. Soybean being a highly nutrient-exhaustive crop requires higher amounts of nutrients, particularly phosphorus for its optimum production. Thus, the present investigation was undertaken. Methods: A field experiment was conducted for three years to study the effect of phosphorous application on yield and P uptake by soybean in different cropping systems. There were three cropping systems which were kept in main plots and five P levels viz., 0, 20, 40, 60 and 80 kg P2O5 ha-1 applied to soybean which were kept in the sub plot. Result: Application of 80 kg P2O5 ha-1 resulted in highest mean seed yield of soybean (20.9 qha-1) but significant response was observed up to 40 kg P2O5 ha-1 (19.8 qha-1) only. Highest mean seed P uptake of soybean was observed under application of 80 kg P2O5 ha-1. The mean seed yield, stover yield and P uptake of soybean was not affected significantly under different cropping systems. The interaction effects of cropping system and applied P levels were however non-significant. A significant build-up of available P in surface soil over control was observed under 80 kg P2O5 ha-1 level.

Effect of seed coating on the yield of soybean Glycine max (L.) Merr.

Enhanced seeds, e.g. dressed, encrusted or pelleted seeds, are often sown in agricultural practice. These treatments play a different role depending on the type and chemical composition of the preparation. The aim of the experiment was to compare the effectiveness of three coatings (B – chitosan, C – chitosan + alginate/jojoba oil/E and D – chitosan + alginate/PEG) applied to soybean seeds in comparison to control (A). The study was carried out in three cultivars: Annushka, Mavka and Smuglyanka. The coatings did not differentiate seed yield in 2018 due to favourable weather conditions. The use of coating D in the following years increased seed yield by 0.46 t/ha in 2019 and by 0.51 t/ha in 2020 compared to control. The obtained results allow concluding that coating D was the most effective in soybean cultivation. The field emergence capacity, plant density as well as the SPAD (soil plant analysis development) and LAI (leaf area index) indices were significantly increased compared to control as a result of this coating application. The g<sub>s</sub> index (stomatal leaf conductance) was significantly reduced. The cv. Smuglyanka yields were significantly higher compared to cvs. Mavka and Annushka, by 0.32 t/ha and 0.85 t/ha, respectively. The difference in seed yield between 2018 and 2019 was 0.81 t/ha.

Abundance and Symbiotic Rhizobia Colonizing Soybean (Glycine max) in Soils of Kakamega County, Western Kenya

Identification of effective indigenous rhizobia strain nodulating soybean (Glycine max) was conducted in Kakamega County, Western Kenya. This study was carried out to establish the abundance and symbiotic efficiency of native rhizobia strains in soil samples collected from 12 sites in four agroecological zones within the region. The MPN enumeration technique was used to estimate the indigenous rhizobia numbers and showed that 54% of the sampled sites had high abundance. Rhizobia number in the soil collected from the six regions varied from 2.5 × 102 to 3.12 × 103 cells·g− soil. Thirty isolates from the soil collected in the four regions were tested for efficiency using soybean as the trap plant in Leonard jars containing sterilized vermiculite irrigated with nitrogen-free media. Determination of the relationship between rhizobial abundance and symbiotic efficiency with respect to the soil chemical conditions was performed using ANOVA, and the means were compared using Tukey’s honest significant difference test. Inoculation with bacterial isolates significantly ( P < 0.05 ) increased the nodule number and shoot dry weight, while weight of the seeds showed variations across the groups. The soil pH across the 4 regions ranged between 5.41 and 6.52.

Plant Lectins as Insecticidal Agents Against Cotton Leafworm Spodoptera littoralisand Their Potential Applications in Crop Protection

Background: The production of insecticidal proteins is one of the greatest vital direct defense responses in plants against the attack by phytophagous insects. One particular class of the entomotoxic proteins present in many plant species is lectins that is used against a wide range of important insects. Aim: The insecticidal activity of two lectins from seeds of white kidney bean (Phaseolus vulgaris) and soybean (Glycine max) on the growth, development and survival of the cotton leafworm, Spodoptera littoralis Boisd. (Lepidoptera: Noctuidae) was studied. Methods: Lectin products were isolated and purified using ammonium sulfate precipitation. Insecticidal activity, growth-inhibitory and physiological effects on the differential haemocyte count of lectin products were evaluated against S. littoralis. In vivo effects of lectin products on some S. littoralis enzymes were also determined. Results: Lectin contents in 250 g seeds were 700 and 500 mg in soybean and white kidney bean, respectively. The total protein concentrations were 160-163 and 118-120 mg/mL in soybean and white kidney bean, respectively. The insecticidal activity of lectin products exerted a strong effect on the larval weight gain and the reduction of the weight reached to 75.25%. Furthermore, the other developmental stages of pupation and adult formation were also affected. An elongated larval duration was noticed since larval duration was 24.8 days compared with control (12.0 days). Complete pupation (100%) was recorded in the control; however, a significant decrease in pupation was found to be 54.0% in treated larvae. Lectin products increased the average number of 123.9 haemocytes (123.9) compared to the control (94.4 haemocytes/field). In addition, lectin products inhibited the activity of total proteases, α-amylase, GST and total esterases. Conclusion: It can be concluded that the lectins have high potential in pest control strategies based on their activity towards the cotton leafworm, S. littoralis. These findings suggest that lectin products are interesting tools for integrated pest management in important agriculture crops.

Cookies made from mangrove (Bruquiera gymnorrhiza) fruit and soybean (Glycine max) flour

Demak is an area in Indonesia with a high stunting rate of 50.28%. Mangrove fruit, a major regional commodity, should be used as a basis for Supplementary Feeding Program (SFP) cookies to overcome stunting in Demak. This study was aimed to analyse the nutritional content, nutrition absorption inhibiting agents, and acceptance of mangrove fruit cookies. This research used a completely randomized sample with a one-factor design to formulate mangrove and soybean flour. The formulations used were F1 (60%: 40%), F2 (70%: 30%), F3 (80%: 20%), and F0 (100% wheat flour) as the control group. The best formula of mangrove and soybean cookies was F2 with average energy 479.44 kcal/100 g, protein 11.70 g/100 g, fat 23.93 g/100 g, carbohydrate 54.31 g/100 g, zinc 2.68 mg/100 g, iron 6.72 mg/100 g, calcium 659.03 mg/100 g, HCN 1.77 ppm, and tannin 101.9 mg/100 g. The best cookie formulation that met the SFP quality requirements was F2, with a composition of 70% mangrove fruit flour and 30% soy flour.

Antixenosis in Glycine max (L.) Merr against Acyrthosiphon pisum (Harris)

To reveal the antixenosis potential against the pea aphid Acyrthosiphon pisum (Harris) (Hemiptera: Aphididae) we analyzed the pea aphid survival and probing behavior, and the quantitative and qualitative variation of flavonoids in the leaves of selected soybean Glycine max (L.) Merr (Fabaceae) cultivars ‘Aldana’, ‘Annushka’, ‘Augusta’, ‘Madlen’, ‘Mavka’, ‘Simona’, ‘Violetta’, and ‘Viorica’. Aphid survival was drastically impeded on all cultivars. The electronic monitoring of aphid probing using the Electrical Penetration Graph (EPG) technique revealed that on all soybean cultivars, A. pisum readily probed into leaf tissues but the probes were usually terminated before reaching vascular tissues, which demonstrates the activity of antixenosis mechanisms in peripheral tissues epidermis and/or mesophyll in soybean leaves. The potency of antixenosis factors differed among soybean cultivars, which was reflected in differences in aphid survival and frequency and duration of phloem sap ingestion. Seven flavonoids were found: apigenin, daidzein, genistein, glycitein, isorhamnetin, kaempferol, and rutin, which occurred in different amount and proportion in individual cultivars. The content of apigenin and genistein in all soybean cultivars studied probably made them relatively unacceptable to A. pisum. Kaempferol in ‘Aldana’ might be responsible for the observed strong antixenosis resistance of this cultivar to A. pisum. The results of our survey provide the first detailed data that can be used for future studies.

Effect of neem fertilizer rates and weed control methods on the growth and yield of soybeans (Glycine max (L.) merrill) in north Central Nigeria

Two field experiments were conducted at the Research Farm of the Ibrahim Badamasi Babangida University, Lapai, Niger State during the 2018 and 2019 rainy seasons to determine the effect of neem fertilizer rates and weed control methods on the growth and yields of soybeans. The experimental treatments were made up of four neem fertilizer rates (0, 50, 100 and 150 kg ha-1) and six weed control methods (pendimethalin at 1.5 kg a.i ha-1 followed by one hoe weeding, pendimethalin at 2.0 kg a.i. ha-1 followed by diuron at 1.5 kg a.i ha-1, weeding once at 3 WAS, weeding twice at 3 and 6 WAS, weed free and weedy check. The experiment was a 3 × 3 factorial experiment laid out in a Randomize complete block design replicated three times. TGX 1448 – 2E variety of soybean was used for the study. Result showed that weed control efficiency was better with the use of 150 kg ha-1 of neem fertilizer, while decrease in weed dry matter was obtained at 50 kg ha-1. Increase in number of leaves and leaf area were encouraged with 150 kg ha-1 of neem fertilizer. Weed free treatments recorded the highest grain yield and 100 seed weight of soybean. Pendimethalin at 1.5 or 2.0 kg a.i ha-1 supplemented with one hoe weeding or diuron at 1.5 kg a.i ha-1 respectively can be an alternative for better control of weeds to obtain greater yield of soybean in the study area.

The Effect of Osmopriming on Germination and Seedling Growth of Glycine Max L. Under Salinity Stress


 
 
 Soybean (Glycine max L.) is a staple food crop in Indonesia. Soybean plants have decreased in quality and production due to salinity factors. Planting media with high salinity conditions have limited potential for plant cultivation, but each plant has different resistance and adaptability. Osmotic priming of seeds or osmopriming could increase plant resistance to salinity. This experiment was conducted at Universitas Muria Kudus Field. The objective of this study was to determine the germination and seedling growth of Glycine max L. as treated by NaCl osmopriming. A randomized complete design was used with two factors. The first factor was osmopriming concentration which consisted of the control, 50 mM (K1), 100 mM (K2), and 150 mM (K3). The seeds of the control did not receive osmopriming NaCl treatment. The second factor was osmopriming duration which consisted of 24 hours (T1) and 48 (T2). The results showed that the treatment of osmopriming with a concentration of 50 mM NaCl for a duration of 24 hours had a positive impact on seed germination under salinity conditions of 150 mM NaCl. However, this did not significantly affect the seedling growth in salinity conditions of 150 mM NaCl.
 
 
 
 Keywords: osmopriming, salinity, soybean
 
 
 
 
 

The CCCH zinc finger family of soybean (Glycine max L.): genome-wide identification, expression, domestication, GWAS and haplotype analysis

BackgroundThe CCCH zinc finger (zf_CCCH) is a unique subfamily featured one or more zinc finger motif(s) comprising of three Cys and one His residues. The zf_CCCH family have been reported involving in various processes of plant development and adaptation.ResultsIn this study, the zf_CCCH genes were identified via a genome-wide search and were systematically analyzed. 116 Gmzf_CCCHs were obtained and classified into seventeen subfamilies. Gene duplication and expansion analysis showed that tandem and segmental duplications contributed to the expansion of the Gmzf_CCCH gene family, and that segmental duplication play the main role. The expression patterns of Gmzf_CCCH genes were tissue-specific. Eleven domesticated genes were detected involved in the regulation of seed oil and protein synthesis as well as growth and development of soybean through GWAS and haplotype analysis for Gmzf_CCCH genes among the 164 of 302 soybeans resequencing data. Among which, 8 genes play an important role in the synthesis of seed oil or fatty acid, and the frequency of their elite haplotypes changes significantly among wild, landrace and improved cultivars, indicating that they have been strongly selected in the process of soybean domestication.ConclusionsThis study provides a scientific foundation for the comprehensive understanding, future cloning and functional studies of Gmzf_CCCH genes in soybean, meanwhile, it was also helpful for the improvement of soybean with high oil content.

Use of Limiting Nutrients for Reclamation of Non-responsive Soils in Northern Ghana

A better understanding of soil fertility factors that constraint positive crop response to fertilizer inputs will facilitate the improvement of soil nutrient management. In this study, a nutrient omission trial was carried out in a greenhouse condition to identify soil chemical properties limiting in non-responsive soils and to ascertain their effect on soybean (Glycine max) production. The treatments evaluated were control (distilled water), complete nutrient solution (all nutrients), and complete solution with the omission of each of N, P, K, Ca, Mg, and S and micronutrients (Fe, Zn, Cu, Mn, B, and Mo) arranged in a completely randomized design with three replications. After the greenhouse study, the identified limiting nutrients were tested with or without FertiSoil (commercial compost) in a 3 year field experiment. Results of the soil analyses showed low fertility status of the non-responsive soils. The sufficiency quotient index revealed non-responsive soils in all the sites to be predominantly limiting in P and K. The occurrence of other limiting nutrients was also identified: Pishegu (Zn, B), Serekpere (Mg, S), Daffiama Saapare (Ca, Mg, S), and Naaga (Mg, S, Zn, B). The nutrient inputs positively influenced soybean yield response in all the locations. The application of PKZnB with FertiSoil and FertiSoil alone significantly increased soybean grain yields by 585 and 477 kg ha−1, respectively, at Pishegu. Soybean grain yields also increased by 585, 573, and 364 kg ha−1 under the FertiSoil, PKMgS + FertiSoil, and PKMgS applications at Serekpere, respectively. At Daffiama Saapare, the highest (103%) percent increase in soybean grain yield was recorded from the combined application of PKMgSCa and FertiSoil. However, the application of FertiSoil and PKMgSCa singly equally increased soybean grain yield by 77%. Percent soybean grain yield increases of 86, 84, and 74% were observed when PKMgSZnB + FertiSoil, PKMgSZnB, and FertiSoil were applied, respectively, at Naaga. In absolute terms, 83% of the fields had a positive response to mineral fertilizer and 93% to FertiSoil and mineral fertilizer + FertiSoil applications. Organic amendment and/or site-specific fertilizer applications are the best options for alleviating poor or no crop responses to inputs and improve productivity on non-responsive soils.

Genome-wide identification of PTI1 family in Setaria italica and salinity-responsive functional analysis of SiPTI1\u20135

BackgroundPTI1 (Pto-interacting 1) protein kinase belongs to the receptor-like cytoplasmic kinase (RLCK) group of receptor-like protein kinases (RLK), but lack extracellular and transmembrane domains. PTI1 was first identified in tomato (Solanum lycopersicum) and named SlPTI1, which has been reported to interact with bacterial effector Pto, a serine/threonine protein kinase involved in plant resistance to bacterial disease. Briefly, the host PTI1 specifically recognizes and interacts with the bacterial effector AvrPto, which triggers hypersensitive cell death to inhibit the pathogen growth in the local infection site. Previous studies have demonstrated that PTI1 is associated with oxidative stress and hypersensitivity.ResultsWe identified 12 putative PTI1 genes from the genome of foxtail millet (Setaria italica) in this study. Gene replication analysis indicated that both segmental replication events played an important role in the expansion of PTI1 gene family in foxtail millet. The PTI1 family members of model plants, i.e. S. italica, Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), maize (Zea mays), S. lycopersicum, and soybean (Glycine max), were classified into six major categories according to the phylogenetic analysis, among which the PTI1 family members in foxtail millet showed higher degree of homology with those of rice and maize. The analysis of a complete set of SiPTI1 genes/proteins including classification, chromosomal location, orthologous relationships and duplication. The tissue expression characteristics revealed that SiPTI1 genes are mainly expressed in stems and leaves. Experimental qRT-PCR results demonstrated that 12 SiPTI1 genes were induced by multiple stresses. Subcellular localization visualized that all of foxtail millet SiPTI1s were localized to the plasma membrane. Additionally, heterologous expression of SiPTI1–5 in yeast and E. coli enhanced their tolerance to salt stress.ConclusionsOur results contribute to a more comprehensive understanding of the roles of PTI1 protein kinases and will be useful in prioritizing particular PTI1 for future functional validation studies in foxtail millet.

Population of Lamprosema indicata and Their Parasitoid in Edamame Soybean Plant

Soybean Glycine max L. Merrill has a strategic position in food security. Soybean varieties are various, such as edamame or commonly called vegetable soybean. The important edamame pest is L. indicata. This pest has a natural enemy in the form of parasitoids that spread at several altitudes. This study aimed to investigate the L. indicata pest population and their parasitoid in edamame soybean plantations at three different altitudes. In this study conducted at three altitudes namely Purwosari (12 masl), Sebanen (814 masl), and Karangbireuh (1295 masl). At each location consist of one plot (1 Ha) and were made into 4 sub-plots exchanging 10 x10m. Also, the yellow pan trap was installed at 8–57 Day After Planting to collect L. indicata and parasitoid. The results showed that L. indicata was higher in Purwoasri than in other locations. This is due to environmental factors namely temperature. The parasitoid of L. indicata was found in all locations, namely Braconidae family (parasitoid of larvae). While the the paarasitoid were collected in yellow pan trap in all locations i.e. Pteromalidae, Diapriidae, Eulophidae, Mymaridae and Entomacis sp.

Structural organization of <em>TFL1</em>-like genes in representatives of the tribe Phaseoleae DC.

The type of stem growth is one of the key features in determining plant architectonics. Stem growth type is an economically important trait. It interconnects with stem length, flowering duration, yield, resistance to lodging, and suitability of mechanized cultivation. Mutations in the TFL1 gene and its homologs have been demonstrated to change meristem indeterminacy across genera. The aim of this work was to characterize and compare the structural organization of TFL1-like genes in representatives of the tribe Phaseoleae (pigeonpea Cajanus cajan, soybean Glycine max, common bean Phaseolus vulgaris, adzuki bean Vigna angularis, mung bean V. radiata, and cowpea V. unguiculata) based on in silico analysis, including analysis of nucleotide sequences, predicted elements in promoter regions, predicted amino acid sequences, putative functional domains and 3D protein structures. We investigated TFL1 (one gene for adzuki bean, four copies for soybean, two copies for other studied species), ATC (two copies for soybean, one gene for other investigated species), and BFT (two copies for soybean, one gene for other studied species) gene family members found in whole-genome sequences databases available for representatives of the tribe Phaseoleae. The presence of duplicated copies for all genes in soybean may be a result of the last genome duplication event during the evolution of this species. Duplication of TFL1 gene to two copies in most of studied species of the tribe Phaseoleae is probably accompanied by the maintenance of the functional state of these genes. The exception is VrTFL1.2 of V. radiata, which likely had lost its functionality. This work broadens the existing data about the number of gene copies, their structural divergence and evolution, and the expected functional differences. This information will be important for understanding the molecular genetic mechanisms underlying the maintenance of indeterminacy in the growth of the shoot apical meristem, as well as in the control of the transition to the reproductive phase of plant development.