The genetic diversity of the world gene pool of collecting samples from the Federal Research Centre of All-Russian Institute of Plant Genetic Resources named after N.I. Vavilov (VIR) was studied in the conditions of the southern forest-steppe zone of Western Siberia. N.I. Vavilov (VIR) by economically valuable traits and grain quality. The study was conducted in four stages as the gene pool became available: 2000-2003, 2007-2008, 2009-2012, 2019-2020. A total of 186 samples were studied. The following varieties recommended by the State Commission of the Russian Federation were used as standards: Altai Niva, Omskaya Amber, Zhemchuzhina Sibiri. Sowing was carried out on May 1516 by fallow in the specialised crop rotation of the durum wheat breeding laboratory of the Omsk Research Center. The material received in the first year was studied in plots with an area of 0.25 m2, and in subsequent years 3 m2. Agroclimatic conditions varied from favourable to contrasting temperature and water regimes. In studies of durum wheat samples from the VIR collection of different origins, sources were identified according to their economic value. Of the diversity, only a small part (10%) is of breeding interest and can be used in hybridisation. The sources of high productivity may be the samples k-59881, k-59888, k-60388, k-60364, k-60366, k-60413, k-61303, k-62657, k-62658, k-63126, k-63160, k-64353, k-64355, k-6386, k-64953, k-61619, k-63821, Sladunitsa, k-66887, k-66886, k-66293, k-66294, k-66519, k-66675, k-64488. In terms of grain quality the following crops stood out: k-59881, k-59889, k-60388, k-60364, k-61117, k-61650, k-62657, k-64353, k-64354, k-64355, k-6386, k-17985, k-63821, Sladunitsa, Iride, k-60410. As sources of resistance to stem rust, we offer k-6386, k-6662, k-46983, k-60410, Iride, k-65353, k-65733, k-65734. According to the complex of traits (high productivity, grain quality and resistance to diseases) are of breeding value samples k-59881 (Russia), k-6386 (Georgia), k-65734 (Syria), Sladunitsa (Ukraine) and Iride (Italy).