Ten years of a long-term field experiment using different strategies of conventional and conservation tillage in SW Germany were evaluated for soil mineral nitrogen (SMN) and soil moisture content. Treatments analysed were combinations of stubble tillage (S) or no stubble tillage with primary tillage P: mouldboard plough, CP: chisel plough, RTT: rototiller, NT: no-till, and VAR: alternating S/P or S/CP. Best management practices were used for crop rotation, fertilisation and plant protection. CP, VAR and NT resulted in 18.7–19.0% gravimetric water content in spring. Partially significantly lower water content was observed in spring under S/P and CP with 18.2%; P and S/RTT resulted in 18.5%. Autumn soil moisture ranged from 15.3 to 15.8% and did not significantly differ between the treatments. Interactions between treatment and depth were not significant. There was no clear trend for spring soil moisture. No treatment showed a particular response to dry or wet season. Total SMN ranged between 8 and 49 kg ha −1 in spring and 5–26 kg ha −1 in autumn; significant effects of the treatments or interactions of treatment × depth did not occur. The effect of the season was most relevant for the amount of SMN, probably as a result of different conditions for mineralisation. Downward movement of nitrogen in spring was low in all treatments if catch crops were grown in the previous autumn. Overall, different soil tillage methods had little effect on soil moisture and soil SMN under temperate conditions. Seriously adverse effects of any tillage treatment in terms of nitrate leaching or reduced mineralisation seem unlikely. Similarly, the tillage systems did not exhibit obvious advantages or disadvantages in terms of soil water content. An exception may have been the higher water content in autumn in 0–30 cm under high soil disturbance which provided more suitable conditions for germination of the following crop. The decision of whether or not to adopt conservation tillage in temperate climates seems not to be primarily dependent on soil moisture and nitrogen mineralisation or on nitrogen leaching.