AbstractStarch is the leading component of wheat (Triticum spp.) grain and flour. Six hexaploid wheat (T. aestivum L.) near‐isogenic lines (NILs), WQL19WX7A (Reg. no. GS‐192, PI 691847), WQL19WX4A (Reg. no. GS‐193, PI 691848), WQL19WX7D (Reg. no. GS‐194, PI 691849), WQL19WX7A.A4 (Reg. no. GS‐195, PI 691850), WQL19WX7A.7D (Reg. no. GS‐196, PI 691851), and WQL19WX4A.7D (Reg. no. GS‐197, PI 691852), were developed to facilitate studies examining the role of starch amylose and amylopectin on wheat processing and end‐product quality and as parents to develop reduced amylose germplasm. Each NIL lacks a functional copy of one or two of the Waxy granule bound starch synthase I (GBSSI) genes and conversely possesses one or two functional genes. These genetic stocks were produced in ‘Alpowa’ soft spring wheat. The NILs are BC7 sibs, selected among segregating BC7 progeny using allele‐specific primers. The GBSSI null alleles were derived from the waxy cultivar Waxy‐Pen (PI 637779). These NILs are complemented by wild‐type Alpowa and a full waxy (zero amylose) line approved for release, ‘USDA Lori’. These lines were developed at the USDA Western Wheat Quality Laboratory.