Objective To investigate the effect of the silencing RhoGDI2 gene expression by small interfering RNA (siRNA) on the apoptosis and invasion of colon cancer cell lines. Methods With Western blotting analysis and real-time reverse transcription-polymerase chain reaction (RT-qPCR), RhoGDI2 expression of the colon cancer cell lines of LoVo, SW480, CaCo2, DLD1, RKO, the HT-29 was detected. The siRNA of RhoGDI2 with LipofectamineTM2000 was transfected into target cells, comprising siRNA and control groups ; Annexin V/PI staining flow cytometry to detect apoptosis, with Wound healing assay and the Transwell plate migration and invasion was detected. The epithelial-mesenchymal transition (EMT) relevant protein E-cadherin/Vimentin and Snail expression was detected. Results Human colon cancer cell lines RhoGDL2 expression levels decreased in the order of DLD1, of HT29, RKO, CaCo2, LoVo, SW480 ; siRNA inhibited RhoGDI2 expression rate of LoVo cell by 70% ;in silence group, negative control group and blank control group, the apoptosis rates were (2. 67 + 0. 97) % (2.45 ~ 0. 74) % (2. 35 ~ 0. 43 ) % ( P 〉 0. 05 ) ; Wound healing assay and Transwell assay suggested RhoGDI2 silencing could improve migration ; siRNA interference of colon cancer cells downregulatd E-cadherin, but upregulated Vimentin protein of Snail expression. Conclusion siRhoGDI2 could significantly facilitate the cell proliferation, migration, invasion of colon cell. Key words: Small interfering RNA; RhoGDI2; Colon cancer; Epithelial-mesenchymal transition; Cell migration and invasion