Abstract
The molluscicidal activity of Morus nigra fruit, bark and leaf powder against the snail Lymnaea acuminata was time and concentration dependent. Toxicity of fruit powder (96h LC 50 : 166.92 mg/L) was more pronounced in comparison to bark powder (96h LC 50 : 173.17 mg/L) and leaf powder (96h LC 50 : 173.69 mg/L). Ethanolic extracts of M . nigra fruit, bark and leaf was more toxic than their other organic solvent extracts. The molluscicidal activity of ethanolic extract of M . nigra fruit powder (24h LC 50 : 116.23 mg/L) was more effective than the ethanolic extract of bark powder (24h LC 50 : 154.41 mg/L) and leaf powder (24h LC 50 : 139.80 mg/L). The 96h LC 50 of column-purified fraction of M . nigra fruit powder was, 10.03 mg/L whereas that of bark and leaf powder was 8.69 mg/L and 4.97 mg/L, respectively. Column and thin layer chromatography analysis demonstrates that the active molluscicidal component in M . nigra is quercetin (96h LC 50 : 1.11 mg/L), apigenin (96h LC 50 : 1.92 mg/L) and morusin (96h LC 50 : 2.12 mg/L), respectively. Co-migration of quercetin (R f 0.49), apigenin (R f 0.51) and morusin (R f 0.52) with column-purified fruit, bark and leaf of M . nigra on thin layer chromatography demonstrates same R f value. The present study indicates that M . nigra may be used as potent source of molluscicides against the snail Lymnaea acuminata .
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