This article presents a change in the indicators of a compression ignition (CI) engine by replacing conventional diesel fuel (D100) with pure chicken fat (F100) and mixtures of these fuels. Mixtures of diesel and fat with volume ratios of 70/30, 50/50 and 30/70 were used. Research of the fuel properties was conducted. In order to reduce the fuel viscosity, blends of fat and diesel were heated. The experimental research was conducted at different engine loads with exhaust gas recirculation (EGR) both off and on. The conducted analysis of the combustion process revealed a significant change in the rate of heat release (ROHR) when replacing diesel with chicken fat. Chicken fat was found to increase the CO2 and CO emissions, leaving hydrocarbon (HC) emissions nearly unchanged. Having replaced the D100 with diesel and chicken fat mixtures or F100, a significant reduction in smoke and nitrogen oxide (NOx) emissions was observed when EGR was off. When EGR was on, the smoke level increased, but the blends with chicken fat reduced it significantly, and the increased fat content in the fuel mixture reduced the NOx emissions. The engine’s brake specific fuel consumption (BSFC) increased while the brake thermal efficiency (BTE) decreased, having replaced conventional diesel with chicken fat due to differences in the fuel energy properties and the combustion process.