In order to investigate the thermal stratification characteristics induced by pool fire and canyon cross wind, a set of tests were carried out in a 1:20 model tunnel. Experimental parameters were the canyon wind speed, longitudinal fire location and fire size. Results show that the dimensionless smoke layer thickness h∗ (the ratio of smoke layer thickness to hydraulic diameter) downstream the fire generally increases with increasing canyon wind speed until reaching the maximum value, and h∗ of a smaller-scale fire is slightly greater than that of a larger-scale fire at most canyon wind speeds. However, h∗ value upstream the fire is almost between 0.2 and 0.3 (h∗ = 0 is not considered). The smoke stratification downstream the fire gradually worsens with increasing canyon wind speed and decreasing distance between the fire source and the upstream portal Df in the cases of 0.194 ≤ Df∗ < 1 (Df∗ represents the ratio of Df to the tunnel length), while the smoke stratification upstream the fire is always obvious unless there is no smoke. As Df increases, the critical Froude number for judging the smoke stratification status decreases first for 0 ≤ Df∗ < 0.028 and then increases for 0.028 ≤ Df∗ < 0.306, and tends to be a constant value for 0.306 ≤ Df∗ < 1.