Similar Stiffness Research Articles

Vibration Energy Color Doppler Imaging (VECDI) in Evaluating the Effect of Screw Fixation on Sacroiliac Joint Stiffness: A Prospective Pilot Study

BackgroundThe sacroiliac joints (SIJ) are specialized articulations in the pelvis that allow load transfer between the upper and lower body. Traumatic pelvic disruption often requires surgical fixation of at least one of these joints. Subsequent SIJ pain is associated with asymmetries in joint laxity or stiffness. This pilot study examines SIJ stiffness in patients with intact sacroiliac screw fixation after posterior pelvic ring injuries. This information will prove valuable to informing surgeons about technique efficacy in SIJ injury stabilization.Questions/purposesThis pilot study examined SIJ stiffness in patients with intact sacroiliac screw fixation to: (1) establish vibration energy color Doppler imaging (VECDI) SIJ intra-rater reliability; (2) measure SIJ stiffness in subjects following surgical fixation using VECDI; (3) compare stiffness data between post-surgical and healthy control subjects; (4) evaluate the relationship between stiffness data and pain and disability scores.Methods13 reliability and 19 experimental subjects were tested using VECDI. Subjects were placed into a side-lying position on top of a shaker apparatus that transmitted vibration energy to the pelvic ring while color Doppler images were taken from the bilateral posterior SIJ. One investigator performed SIJ measurements on healthy subjects to establish reliability and then evaluated post SIJ fixation stiffness in experimental subjects at four-weeks (T1) and eight-weeks (T2). Visual analog scales were used to collect subjective pain scores at each time point.ResultsHealthy subject VECDI values suggested good intra-rater reliability (ICC = 0.819; CI 95% = 0.405–0.945). No significant differences in SIJ stiffness (ΔTU) were observed between healthy and experimental subjects at both time points (p > .05). Pearson correlation coefficients highlighted relationships between current pain at T1 and T2 (p = .004, r = .879), and Oswestry Disability Index (ODI) values at T1 and T2 (p = .003, r = .890).ConclusionsAsymmetric laxity through the SIJ can be associated with pain resulting from either trauma or inherent physiologic variations. Prior to this study, the mainstay of evaluation was subjective indexes, such as the ODI. Here we propose VECDI as a potentially objective tool in SIJ assessment. Following surgical fixation necessitated by trauma, fixation using SI screws demonstrated similar stiffness values through the SIJ at four- and eight-weeks post-operatively when compared to healthy controls. Direct inferences regarding VECDI’s exact sensitivity to SIJ dysfunction cannot be concluded from our investigation due to small sample sizes. Future investigations should include a larger sample size to enhance our understanding of stiffness measurements obtained using VECDI, validate the technique, and determine the time-course of healing from SIJ surgical stabilization.

Nanoparticle shape is the game-changer for blood-brain barrier crossing and delivery through tunneling nanotubes among glioblastoma cells.

Tunneling nanotubes (TNTs) are thin, dynamic, long membrane protrusions that allow intercellular exchanges of signaling clues, molecules and organelles. The presence of TNTs and their involvement as drug delivery channels have been observed in several types of cancer, including glioblastoma. Recently, increased attention has been directed toward nanoparticles (NPs) that can be transported in TNTs. However, few data are available on the role of physical parameters of nanoparticles, such as size, shape, charge and flexibility, in determining their transfer efficiency between cells by TNTs. Here, we focused our attention on NP shape, manufacturing spherical, discoidal and deformable negatively charged lipid-based NPs with sizes <120 nm and similar stiffness. The TNT-mediated transfer of NPs was investigated in 2D and 3D culture models of human glioblastoma cells. The permeability and biocompatibility of the blood-brain barrier (BBB) were also assessed. Results showed that discoidal NPs displayed the highest TNT-mediated transfer efficiency between cancer cells, with a maximum velocity of 69 nm s-1, and a higher endothelial permeability (1.29 × 10-5 cm min-1) across the BBB in an in vitro model. This depends on the NP shape because discoidal NPs have a larger surface area exposed to the flow along the TNT channel. Overall, the results suggest that the shape of NPs is the game-changer for more efficient TNT-mediated transfer between cancer cells, thus introducing a sustainable solution to improve the diffusion rate at which the NPs spread in the tumour microenvironment, opening the possibility of ameliorating drug distribution to difficult-to-reach cancer cell populations.

Metabolic Profiles of Encapsulated Chondrocytes Exposed to Short-Term Simulated Microgravity.

The mechanism by which chondrocytes respond to reduced mechanical loading environments and the subsequent risk of developing osteoarthritis remains unclear. This is of particular concern for astronauts. In space the reduced joint loading forces during prolonged microgravity (10-6g) exposure could lead to osteoarthritis (OA), compromising quality of life post-spaceflight. In this study, we encapsulated human chondrocytes in an agarose gel of similar stiffness to the pericellular matrix to mimic the cartilage microenvironment. We then exposed agarose-chondrocyte constructs to simulated microgravity (SM) for four days using a rotating wall vessel (RWV) bioreactor to better assess the cartilage health risks associated with spaceflight. Metabolites extracted from media and agarose gel constructs were analyzed on liquid chromatography-mass spectrometry. Global metabolomic profiling detected a total of 1205 metabolite features, with 497 significant metabolite features identified by ANOVA (FDR-corrected p-value < 0.05). Specific metabolic shifts detected in response to SM exposure resulted in clusters of co-regulated metabolites, with glutathione, nitrogen, histidine, vitamin B3, and aminosugars metabolism identified by variable importance in projection scores. Microgravity-induced metabolic shifts in gel constructs and media were indicative of protein synthesis, energy and nucleotide metabolism, and oxidative catabolism. Microgravity associated-metabolic shifts were consistent with our previously published early osteoarthritic metabolomic profiles in human synovial fluid, suggesting that even short-term exposure to microgravity (or other reduced mechanical loading environments) may lead to the development of OA. This work further suggests the potential to detect these metabolic perturbations in synovial fluid in vivo to ascertain osteoarthritis risk in astronauts.

Seismic Performance of Shaped Steel Reinforced High‐Strength Concrete Walls: Cyclic Loading Test and Analytical Modeling

ABSTRACTThe thickness of the bottom shear walls in tall and super‐tall structures is often designed too large to meet the limit requirements of axial compression ratios, which increases the weight and cost of the structure. To address this issue, high‐strength concrete (HSC) and shaped steel are combined to form composite shear wall members. This paper focuses on seismic performance and analytical hysteresis model of steel reinforced HSC shear walls (SRHCW) under high axial compressive load. Firstly, the reversed cyclic test was conducted to investigate the influence of concrete strength and the steel ratio on the seismic performance of SRHCWs by comparing the failure mechanism, hysteretic curves, stiffness degradation, ductility, energy dissipation, and steel bar strain variation of each specimen. Then, a hysteresis model for SRHCWs was established, and the model can be used for the nonlinear analysis and seismic performance evaluation of SRHCWs. Finally, the accuracy of the proposed hysteresis model was evaluated by the experimental data. The experimental results show that under high axial compression ratio, the interstory drift ratio capacity of SRHCWs can reach 3.03% showing excellent deformation performance. The wall specimens built with different strength concrete and shaped steel ratios exhibited similar strength, deformation, and initial stiffness, indicating that the steel ratio of the wall can be effectively reduced by upgrading the concrete strength of the wall specimens while ensuring its seismic performance. The analytical model can well predict the hysteresis force–deformation curves of SRHCWs under high axial load ratios.

Bending stiffness of Toxoplasma gondii actin filaments.

Actin is essential for the survival and pathogenicity of the Apicomplexan parasite Toxoplasma gondii, where it plays essential functions in cargo transport, invasion, egress, and organelle inheritance. Recent work has shown that, unlike vertebrate skeletal muscle actin, purified T. gondii actin filaments (TgAct1) can undergo rapid treadmilling, due to large differences in the barbed- and pointed-end critical concentrations, rapid subunit dissociation from filament ends, and a rapid nucleotide exchange rate constant from free monomers. Previous structural analysis suggested that the unique assembly properties of TgAct1filaments may be a functional consequence of reduced contacts between the DNAse-1 binding loop (D-loop) of a filament subunit and its adjacent, long-axis subunit neighbor. Because the D-loop makes stabilizing interactions between neighboring subunits, it has been implicated in regulating the mechanical properties of actin filaments. In this study, we measured the bending persistence length (LB) of TgAct1 filaments and the filament length distribution. We found that despite compromised intersubunit D-loop contacts, TgAct1 filaments have similar bending stiffness and thermodynamic stability as vertebrate actin filaments. Analysis of published cryoEM image density maps indicates that TgAct1 filaments retain a stabilizing inter-subunit salt bridge between E168 and K62 and reveals visible density between Y167 and S61 of adjacent filament subunits, consistent with a conserved cation binding site proximal to the D-loop, as initially identified in vertebrate skeletal muscle actin filaments. These results favor a mechanism in which weak D-loop interactions compromise TgAct1 subunit incorporation at filament ends, while minimally affecting overall subunit interactions within filaments.

Bending Performance of F-Type Socket Joints for Rectangular Pipe Jacking Tunnels with Longitudinal Connectors

Joints are the weakest part of rectangular pipe jacking tunnels, and the structural form of the joint is closely related to its bending resistance. In this work, the F-type socket joint of a rectangular pipe jacking tunnel is selected as the object of study. The bending mechanical properties of the joints connected by steel screws and those connected by bent bolts are compared via a three-point bending test. The results show that the two longitudinal connection joints have similar bending stiffnesses. Compared with the bent bolt connection joint, the steel screw connection joint has better toughness, and the load at which the joint enters the plastic stage and the bearing capacity are increased by 0.47 times and 1.02 times, respectively. The failure modes of the joints connected by steel screw connections and those connected by bent bolts are crushing of the concrete of the top plate and cracking of the concrete above the screw holes, respectively. When a bent bolt connection is used, the reinforcement at the screw hole should be locally strengthened, or ultrahigh-performance concrete (UHPC) should be used at the screw hole to improve the load-bearing capacity of the joint.

Ex Vivo Biomechanical Comparison of Four Techniques to Tibiotarsus Osteosynthesis in Adult Laying Hens (Gallus gallus domesticus).

To assess the biomechanical parameters of intact tibiotarsi (INT) and tibiotarsi with a 5-mm segmental diaphyseal defect repaired using four osteosynthesis techniques: a locking plate (LP), a plate-rod combination, an external skeletal fixator (one end-threaded positive-profile pin per fragment) with an intramedullary pin tie-in (TIF 1), and an external skeletal fixator (two end-threaded positive-profile pins per fragment) with an intramedullary pin tie-in (TIF 2). Sixty tibiotarsi from 30 adult laying hens were allocated into five groups for nondestructive dynamic torsion and four-point bending tests, followed by failure tests. Nondestructive dynamic tests evaluated stiffness over time in torsion and bending. Torsion destructive tests provided maximum torque and rotation values, whereas the four-point bending tests provided the yield load, maximum bending load, and maximum displacement. The INT group showed higher torsional stiffness and maximum torque but similar bending stiffness, torsional strength, and bending strength in one or more groups. LP and TIF 2 exhibited the highest similarity frequencies among the treatment groups, whereas the TIF 1 group displayed lower stiffness and strength for most of the evaluated parameters. Similar results for LP and TIF-2 groups suggest the biomechanical equivalence of these methods for tibiotarsal osteosynthesis in adult hens.

Integrated Compression Screw Versus Unicortical Locking Screw for Fixing the Dorsal Critical Corner in Distal Radius Fractures: A Biomechanical Study.

Although studies have demonstrated that locked screws with a length of 75% of the radius width are sufficient for the treatment of extra-articular fractures of the distal radius, the application of this principle to intra-articular fractures is less well-understood. This study aimed to evaluate the biomechanical properties of different types of volar plate fixation constructs for the treatment of distal radius fractures in the presence of a dorsal critical corner fragment. A dorsal critical corner fracture was created in 18 synthetic distal radius models. The fragment was stabilized with one of three constructs: a posteroanterior integrated compression screw (group 1) or volar locking screws with a length of 90% or 75% of the volar/dorsal width of the radius (groups 2 and 3, respectively). For the biomechanical evaluation, a load was applied to the lunate facet. Fixation stiffness and loads to produce clinical and catastrophic failures were compared among the groups. The stiffness (N/mm) was 67.8 (SD, 14.7), 64.9 (SD, 8.63), and 65.8 (SD, 36.02) for groups 1, 2, and 3, respectively. The load required to generate a catastrophic displacement was 532.9 (SD, 142.32), 307.4 (SD, 101.51), and 230.8 N (SD, 77.68) for groups 1, 2, and 3, respectively. The load required to produce a 2-mm translation of the fixed fragment was 127.9 N (SD, 28.8) for group 1, 119.7 (SD, 11.78) for group 2, and 127.6 N (SD, 46.2) for group3. Significantly greater load is required for catastrophic failure after fixation of a dorsal critical corner fracture with an integrated compression screw; however, it provides similar stiffness and load to failure for 2 mm of translation. For the treatment of intra-articular distal radius fractures with dorsal critical corner fragments, an integrated compression screw may be considered for rigid fixation of the fragment to support early return to daily activities.

Casein network formation at oil–water interfaces is reduced by [formula omitted]-casein and increased by Ca[formula omitted]

Mixtures of bovine caseins can serve as a benchmark for understanding the functionality of microbial-based recombinant caseins at oil–water interfaces. In this work we show that, in the presence of Ca2+, the individual casein fractions form viscoelastic networks at the oil–water interface with comparable stiffness. In the absence of Ca2+, αs2- and β-casein interfacial network formation was strongly inhibited over the full deformation regime. For αs1-casein, the network stiffness was increased in the absence of Ca2+ at small deformations (<15%), but at large deformations (>50%) it was completely disrupted, to a similar stiffness as αs2- and β-casein. The interfacial structure formed by κ-casein was largely unaffected by Ca2+ due to limited phosphorylation. We hypothesize that the differences between calcium-sensitive caseins lie in the conformation they assume at the interface. Both αs2- and β-casein adsorb in a train-tail conformation with a tail extending into the aqueous bulk phase, whereas αs1-casein adsorbs in a loop-train conformation, with a loop that extends less into the bulk phase. The tail-train configuration is hypothesized to increase the inter-molecular Ca2+ bridging thereby increasing the interfacial stiffness of αs2- and β-casein.Blending the casein fractions revealed a strong negative effect of β-casein on the interfacial modulus, which was more pronounced at a higher concentration. The presence of Ca2+ remained important for interfacial network formation of a casein blend. Without Ca2+, the interfacial network was less stiff, more viscous, and behaved like a 2d polymer solution.With this work we showed that casein interfacial network formation at oil–water interfaces is mediated by Ca2+ bridging. Blending the different casein fractions decreased the interfacial viscoelastic properties through the presence of β-casein. These results indicate that future work on recombinant caseins should focus on single genetic variants, since a blend of variants will likely decrease interfacial functionality.

Sustained Dynamic Compression Device Adaptively Compensates to Bone Resorption Following Arthrodesis in Ovine Hindlimb Model

Category: Midfoot/Forefoot; Basic Sciences/Biologics Introduction/Purpose: Charcot neuroarthropathy (CN) is a degenerative disease, which often impacts the midfoot and ankle, potentially leading to dislocations/fractures in the boney structures of the foot. The degenerative nature of this disease challenges the surgical intervention (beaming of the medial and lateral columns) for this condition by decelerating the fusion time, exposing the hardware to increased loading magnitude and duration, ultimately leading to increased hardware failure rate in these patients/procedures. To address this challenging patient population, a device was designed to apply sustained dynamic compression for use in midfoot fusion. While similar devices have been successfully implemented clinically in tibiotalocalcaneal fusion, this study sought to confirm mechanical durability via bench top testing and efficacy of this dynamic device in an ovine model of midfoot arthrodesis. Methods: A 7 x 100 mm dynamic device and an equivalent sized static device were used in this study. This 2x2 study divided the thirty-two mature Rambouillet Cross ewes into four groups based on bone quality (normal vs depleted) and device type (static vs dynamic) (CSU IACUC #1199). A pre-established model was utilized to reduce the bone quality of the target limb prior to arthrodesis via external fixation unloading, which has previously demonstrated a 29% decrease in bone mineral density. Multi-joint arthrodesis surgery was performed through the calcaneus-tarsus-metatarsal complex, followed by serial radiographic evaluations every four weeks. Sheep were euthanized 8-months postoperatively for biomechanical testing, micro-computed tomography analysis, and histology assessment of bone-device constructs. The study was conducted under strict ethical guidelines and approved by the Colorado State University Animal Care and Use Committee. Separate benchtop testing was conducted to confirm resorption capacity of both static and dynamic devices. Results: In vivo radiographic assessment revealed no significant differences in bone resorption/joint settling distances across bone quality at all timepoints (p≥.40), with ≥52.3% of total resorption/settling occurring within the first two months post-implantation. At the end of the 8-month study period, the normal and depleted bone quality samples exhibited 2.2 and 1.6mm of total resorption (4mm maximum device capability), respectively (p=.40). Four-point bending of treated calcaneus-tarsus-metatarsus constructs revealed no significant differences between device type or bone quality (p≥0.21). MicroCT assessments revealed significantly increased arthrodesis bone volume fraction (p=.036,Figure1) and mean density bone volume (p=.041,Figure1) in the dynamic group as compared to the static group. Benchtop testing revealed the dynamic device maintained compression through its 4mm design target; whereas the static device lost all compression within 0.5mm. Conclusion: These data illustrate that bone resorption of less than 1mm will eliminate all compressive force when using static fixation hardware, highlighting the benefit of this dynamic device to apply the requisite continued interfragmentary compression in this clinically challenging situation. This capability was confirmed by the in vivo radiographic measurements of dynamic device movement, similar biomechanical stiffness values, and increased resultant arthrodesis bone volume as measured by microCT. These benchtop and preliminary data highlight the capability of a dynamic device to compensate for bony resorption/joint settling in a multiple segment arthrodesis procedure for patients with both good and poor bone quality.

3D melt blowing of Elastollan thermoplastic polyurethane for tissue engineering applications: A pilot study

Scaffolds, in addition to being biocompatible, should possess structural and mechanical properties similar to the natural tissues they intend to replace. Many tissue engineering applications require porous 3D scaffolds characterized by unique microfibrous organization and mechanical anisotropy. Manufacturing process principles and process parameter-biomaterial interactions ultimately govern the properties that can be achieved in the scaffold. In this study, we investigate a recently developed nonwoven scaffold fabrication process, 3D melt blowing (3DMB), for processing Elastollan®, a thermoplastic polyurethane with basic mechanical properties suitable for musculoskeletal tissue engineering. The range of feasible processing parameters was screened and the effects of two sets of critical process parameters (fiber deposition offset and surface velocity of the collector) that produced contrasting scaffold morphologies were assessed. Results showed that scaffolds of Group B that were fabricated at the higher fiber deposition offset (90 %) and higher surface velocity of the collector (6 × 105 mm/min) possessed significantly smaller fiber diameter and higher porosity and degree of fiber alignment along the principal direction of collector rotation during 3DMB (all p < 0.05) compared to Group A scaffolds (fabricated at 50 % offset and 1 × 105 mm/min surface velocity). Although both groups possessed similar tensile stiffness, the elongation at failure was significantly different (p < 0.0001). The higher elongation at failure of Group B correlated with the higher degree of fiber alignment in these scaffolds. In contrast, the more isotropic fibrous organization of Group A contributed to their higher compressive stiffness (p = 0.004). The introduction of NaOH treatment to improve hydrophilicity of the scaffolds resulted in a significant reduction of tensile stiffness of Group A (p < 0.05) but not Group B. This treatment did not significantly affect the elongation at failure or compressive stiffness of both groups. With NaOH-treatment, both groups demonstrated good biocompatibility when seeded with fibroblast cells over 14 days. This study confirms the ability to fabricate via 3DMB, biocompatible, micro-fibrous, Elastollan scaffolds relevant for musculoskeletal tissue engineering.

Glycerol‐plasticized silk fibroin vascular grafts mimic key mechanical properties of native blood vessels

AbstractCardiovascular diseases are a major global health challenge. Blood vessel disease and dysfunction are major contributors to this healthcare burden, and the development of tissue‐engineered vascular grafts (TEVGs) is required, particularly for the replacement of small‐diameter vessels. Silk fibroin (SF) is a widely used biomaterial for TEVG fabrication due to its high strength and biocompatibility. However, the stiffness of SF is much higher than that of native blood vessels (NBVs), which limits its application for vascular tissue engineering. In this study, SF was plasticized with glycerol to produce TEVGs exhibiting similar stiffness and ultimate tensile strength to those of NBVs. The electrospun SF/glycerol TEVGs exhibited mechanical properties comparable to NBVs and supported the in vitro proliferation of essential vascular cells—endothelial and smooth muscle cells. After 5 days of culture, the TEVGs exhibited an endothelial monolayer in the lumen, demonstrating their potential for functional vascular tissue regeneration. Our study demonstrates the feasibility of producing TEVGs from SF with tailored mechanical properties, paving the way for more functional and durable TEVGs for future clinical applications.

Mechanical Properties and Economic Analysis of Fused Filament Fabrication Continuous Carbon Fiber Reinforced Composites.

Additive manufacturing of composites offers advantages over metals since composites are lightweight, fatigue and corrosion-resistant, and show high strength and stiffness. This work investigates the tensile and flexural performance of continuous carbon-fiber reinforced (CCF) composites with different guide angles and number of layers. The cost and printing time analyses were also conducted. Tensile specimens with a contour-only specimen and one CCF layer with a 0° guide angle exhibited nearly comparable strength values. Increasing the number of CCF layers enhances the tensile properties. For the identical cost and reinforcement amount, 0°/0° provides a higher tensile strength and elastic modulus compared with 15°/-15°. The same phenomenon was observed for 15°/0°/-15° and 0°/0°/0°. The samples with one and two reinforcement layers had similar stiffness and maximum load values for flexural tests. For the samples with four layers, there was a considerable improvement in stiffness but a minor decrease in the maximum load.

Conceptual design of a prestressed precast UHPC-steel hybrid tower to support a 15 MW offshore wind turbine

Ultra-high-performance-concrete (UHPC) possesses favorable mechanical properties and economy efficiencies and has the potential to be used as the main material to construct tower structures of offshore wind turbines (OWTs). Then the shortages of widely used pure steel OWT towers such as relatively small lateral stiffness, weak fatigue behavior and corrosion resistance can be avoided. In this work, a new type of hybrid tower structure combining a prestressed UHPC tube at the bottom and an upper steel tube is proposed to support a large-size 15 MW OWT. Detailed and carefully refined finite element (FE) analysis by ABAQUS was conducted to explore the dynamic performance of the proposed hybrid tower. The modal properties, dynamic responses, ultimate strength, fatigue strength, and economy performance of the hybrid tower were comprehensively evaluated. It shows that with a similar lateral stiffness but greatly reduced material and maintenance costs compared to a reference steel tower, the hybrid tower has enough ultimate and fatigue strength to resist huge environmental loads caused by extreme winds, waves and earthquakes.

Visceral pleura mechanics: Characterization of human, pig, and rat lung material properties

Pulmonary air leaks are amongst the most common complications in lung surgery. Lung sealants are applied to the organ surface and need to synchronously stretch with the visceral pleura, the layer of tissue which encompasses the lung parenchymal tissue. These adhesives are commonly tested on pig and rat lungs, but applied to human lungs. However, the unknown mechanics of human lung visceral pleura undermines the clinical translatability of such animal-tested sealants and the absence of how pig and rat lung visceral pleura compare to human tissues is necessary to address. Here we quantify the biaxial planar tensile mechanics of visceral pleura from healthy transplant-eligible and smoker human lungs for the first time, and further compare the material behaviors to pig and rat lung visceral pleura. Initial and final stiffness moduli, maximum stress, low-to-high strain transition, and stress relaxation are analyzed and compared between and within groups, further considering regional and directional dependencies. Visceral pleura tissue from all species behave isotropically, and pig and human visceral pleura exhibits regional heterogeneity (i.e. upper versus lower lobe differences). We find that pig visceral pleura exhibits similar initial stiffness moduli and regional trends compared to human visceral pleura, suggesting pig tissue may serve as a viable animal model candidate for lung sealant testing. The outcomes and mechanical characterization of these scarce tissues enables future development of biomimetic lung sealants for improved surgical applications. Statement of significanceSurgical lung sealants must synchronously deform with the underlying tissue and with each breath to minimize post-operative air leaks, which remain the most frequent complications of pulmonary intervention. These adhesives are often tested on pig and rat lungs, but applied to humans; however, the material properties of human lung visceral pleura were previously unexplored. Here, for the first time, the mechanics of human visceral pleura tissue are investigated, further contrasting rarely acquired donated lungs from healthy and smoking individuals, and additionally, comparing biaxial planar material characterizations to animal models often employed for pulmonary sealant development. This fundamental material characterization addresses key hindrances in the advancement of biomimetic sealants and evaluates the translatability of animal model experiments for clinical applications.

Perturbation approaches to achieving diverse and competitive designs in topology optimisation

Utilising topology optimisation to generate diverse and competitive structures enables designers to create elegant and efficient designs according to their aesthetic intuition and functional needs. This study proposes load and support perturbation approaches based on the bi-directional evolutionary structural optimisation (BESO) method to achieve diverse designs. During the optimisation process, noise is introduced to the sensitivity calculations by randomly perturbing the load angles or material properties near the supports, leading to a variety of local optima. Numerical results demonstrate that the proposed approaches are capable of creating designs with similar stiffness but distinct geometries. In addition, the diversity of the obtained solutions can be controlled by utilising different perturbation functions. This work is of significant practical importance in both architecture and engineering, where multiple design options of high structural performance are in demand.

Effect of aliovalent substitution on the local structure of CaKFe4As4 superconductor

We have investigated the local structure of the iron-based CaKFe4As4superconductor featuring distinct aliovalent substitutions at the Ca and K sites, that is CaKFe4As4, CaK0.9Sr0.1Fe4As4, CaK0.9Ba0.1Fe4As4and Ca0.9Na0.1K0.9Ba0.1Fe4As4. Temperature-dependent Fe K-edge extended x-ray absorption fine structure (EXAFS) measurements are used to determine the near-neighbors bondlengths and their stiffness. The EXAFS analysis reveals that the Fe-As bondlength undergoes negligible changes by substitution, however, the Fe-Fe bondlength and the As height are affected by the Sr substitution. The superconducting transition temperatures of CaK0.9Sr0.1Fe4As4and CaK0.9Ba0.1Fe4As4are very similar even if the mean As heights are significantly different suggesting that the anion height may not be a unique parameter to describe the superconductivity in CaKFe4As4. The mean As heights show a peculiar temperature dependence characteristic of CaKFe4As4system. Furthermore, the temperature-dependent mean square relative displacements reveal similar Fe-Fe bond stiffness in all samples, instead the Fe-As bond is substantially stiffer in case of CaK0.9Sr0.1Fe4As4. The local structure results are discussed in relation to the differing transport properties of aliovalent substituted 1144 superconductor.

Experimental and numerical study on cyclic behavior of precast segmental railway bridge columns with lap-spliced rebar connections using UHPC wet joints

A novel lap-spliced connection with ultra-high performance concrete (UHPC) was proposed to connect precast railway bridge column segments with footings or cap beams. Two 1:3 scaled specimens were tested under cyclic loads. Specimen PC-LS was a precast column with lap-spliced rebar connection and UHPC wet joints. Specimen CIP was a monolithic column as a reference specimen. The test results revealed that the plastic hinge region of the precast specimen shafted to the upper surface of the UHPC wet joint. The failure mode of the precast specimen was exhibited as the crushing of the core concrete and the buckling/fracture of the longitudinal rebars. Specimen PC-LS experienced larger lateral force, initial stiffness, comparable ductility, and energy dissipation compared to specimen CIP. Additionally, the validated finite-element (FE) models considering the bond-slip effect between concrete and reinforcements were developed. The parametric studies were carried out to further investigate the cyclic behavior of specimen PC-LS. Analytical results demonstrated that the variation of the compressive and tensile strength of UHPC showed little influence on the cyclic behavior of the precast segmental column, which indicated that UHPC with natural curing and lower volume content steel fibers could be used as the wet joint. The precast segmental column had similar lateral force and stiffness with the cast-in-place column when the lap splice length was 10 times of the rebar diameter.

Design optimization of lightweight automotive seatback through additive manufacturing compression overmolding of metal polymer composites

With the growing demand for enhanced automotive fuel efficiency and environmental sustainability, there is a need for lightweighting automotive components through innovative design and manufacturing processes. This study leverages a combination of numerical iterative design optimization and hybrid additive manufacturing–compression molding (AM-CM) technique for metal polymer composites to lightweight an automotive seatback. The AM-CM process enables robust mechanical interlocking between metals and composites, boasting high stiffness and strength with low overall density. Replacing metallic components with such metal polymer composites allows for comparable mechanical performance while significantly reducing the overall weight. First, the automotive seatback design space is reduced to critical load carrying regions using topology optimization and high stress concentration areas are identified using finite element analysis. Next, a lightweight metal polymer subcomponent is designed for a high stress concentration region. The full seatback frame with spatially heterogeneous material-specific design is then iteratively optimized to enable enhanced stiffness with minimal weight. Overall, the automotive seatback frame designed with location-specific metal, polymer, and metal polymer composite materials weighs 20% less than the metal-only design while exhibiting similar stiffness.

Low-Load Resistance Exercise With Blood Flow Restriction Versus High-Load Resistance Exercise on Hamstring Muscle Adaptations in Recreationally Trained Men.

Kamiş, O, Gürses, VV, Şendur, HN, Altunsoy, M, Pekel, HA, Yıldırım, E, and Aydos, L. Low-load resistance exercise with blood flow restriction vs. high-load resistance exercise on hamstring muscle adaptations in recreationally trained men. J Strength Cond Res XX(X): 000-000, 2024-Low-load resistance exercise with blood flow restriction (BFR-RE) has been suggested as a viable alternative exercise for traditional high-load resistance exercise (HL-RE). However, very little is known about hamstring muscle thickness, stiffness, and strength after BFR-RE. This study aimed to compare the effects of 6 weeks of BFR-RE and HL-RE on hamstring muscle thickness, stiffness, and strength. Twenty-nine recreationally trained men were pair matched and randomly assigned to the BFR-RE (n = 15) and HL-RE (n = 14) groups. The BFR-RE groups performed bilateral lying leg curl exercise (30-15-15-15 reps, 30-second rest between the sets, 30% 1RM) with BFR cuffs (60% of limb occlusion pressure), whereas HL-RE performed the same exercise (3 × 12 reps, 90-second rest between the sets, 70% 1RM) without BFR cuffs. Hamstring muscle thickness, muscle stiffness, isokinetic muscle strength, and 1RM were assessed at baseline and follow-up after completing a 6-week resistance exercise program (3× a week). Hamstring muscle thickness was assessed by ultrasonography, whereas muscle stiffness was evaluated by shear wave elastography. Isokinetic dynamometry and a 1RM strength test were used to determine muscular strength before and after the exercise program. Statistical significance was set at p < 0.05. No significant effect was found for hamstring muscle strength, thickness, and stiffness for group and group × time interaction, and only a significant main effect of time (p < 0.001) was observed for all outcomes. Both groups experienced significant improvements for all outcomes from baseline to follow-up without any between-group differences. In conclusion, results revealed that BFR-RE can provide similar hamstring muscle strength, thickness, and stiffness compared with HL-RE.