Similar Stiffness Research Articles

Casein network formation at oil–water interfaces is reduced by [formula omitted]-casein and increased by Ca[formula omitted]

Mixtures of bovine caseins can serve as a benchmark for understanding the functionality of microbial-based recombinant caseins at oil–water interfaces. In this work we show that, in the presence of Ca2+, the individual casein fractions form viscoelastic networks at the oil–water interface with comparable stiffness. In the absence of Ca2+, αs2- and β-casein interfacial network formation was strongly inhibited over the full deformation regime. For αs1-casein, the network stiffness was increased in the absence of Ca2+ at small deformations (<15%), but at large deformations (>50%) it was completely disrupted, to a similar stiffness as αs2- and β-casein. The interfacial structure formed by κ-casein was largely unaffected by Ca2+ due to limited phosphorylation. We hypothesize that the differences between calcium-sensitive caseins lie in the conformation they assume at the interface. Both αs2- and β-casein adsorb in a train-tail conformation with a tail extending into the aqueous bulk phase, whereas αs1-casein adsorbs in a loop-train conformation, with a loop that extends less into the bulk phase. The tail-train configuration is hypothesized to increase the inter-molecular Ca2+ bridging thereby increasing the interfacial stiffness of αs2- and β-casein.Blending the casein fractions revealed a strong negative effect of β-casein on the interfacial modulus, which was more pronounced at a higher concentration. The presence of Ca2+ remained important for interfacial network formation of a casein blend. Without Ca2+, the interfacial network was less stiff, more viscous, and behaved like a 2d polymer solution.With this work we showed that casein interfacial network formation at oil–water interfaces is mediated by Ca2+ bridging. Blending the different casein fractions decreased the interfacial viscoelastic properties through the presence of β-casein. These results indicate that future work on recombinant caseins should focus on single genetic variants, since a blend of variants will likely decrease interfacial functionality.

3D melt blowing of Elastollan thermoplastic polyurethane for tissue engineering applications: A pilot study

Scaffolds, in addition to being biocompatible, should possess structural and mechanical properties similar to the natural tissues they intend to replace. Many tissue engineering applications require porous 3D scaffolds characterized by unique microfibrous organization and mechanical anisotropy. Manufacturing process principles and process parameter-biomaterial interactions ultimately govern the properties that can be achieved in the scaffold. In this study, we investigate a recently developed nonwoven scaffold fabrication process, 3D melt blowing (3DMB), for processing Elastollan®, a thermoplastic polyurethane with basic mechanical properties suitable for musculoskeletal tissue engineering. The range of feasible processing parameters was screened and the effects of two sets of critical process parameters (fiber deposition offset and surface velocity of the collector) that produced contrasting scaffold morphologies were assessed. Results showed that scaffolds of Group B that were fabricated at the higher fiber deposition offset (90 %) and higher surface velocity of the collector (6 × 105 mm/min) possessed significantly smaller fiber diameter and higher porosity and degree of fiber alignment along the principal direction of collector rotation during 3DMB (all p < 0.05) compared to Group A scaffolds (fabricated at 50 % offset and 1 × 105 mm/min surface velocity). Although both groups possessed similar tensile stiffness, the elongation at failure was significantly different (p < 0.0001). The higher elongation at failure of Group B correlated with the higher degree of fiber alignment in these scaffolds. In contrast, the more isotropic fibrous organization of Group A contributed to their higher compressive stiffness (p = 0.004). The introduction of NaOH treatment to improve hydrophilicity of the scaffolds resulted in a significant reduction of tensile stiffness of Group A (p < 0.05) but not Group B. This treatment did not significantly affect the elongation at failure or compressive stiffness of both groups. With NaOH-treatment, both groups demonstrated good biocompatibility when seeded with fibroblast cells over 14 days. This study confirms the ability to fabricate via 3DMB, biocompatible, micro-fibrous, Elastollan scaffolds relevant for musculoskeletal tissue engineering.

Glycerol‐plasticized silk fibroin vascular grafts mimic key mechanical properties of native blood vessels

AbstractCardiovascular diseases are a major global health challenge. Blood vessel disease and dysfunction are major contributors to this healthcare burden, and the development of tissue‐engineered vascular grafts (TEVGs) is required, particularly for the replacement of small‐diameter vessels. Silk fibroin (SF) is a widely used biomaterial for TEVG fabrication due to its high strength and biocompatibility. However, the stiffness of SF is much higher than that of native blood vessels (NBVs), which limits its application for vascular tissue engineering. In this study, SF was plasticized with glycerol to produce TEVGs exhibiting similar stiffness and ultimate tensile strength to those of NBVs. The electrospun SF/glycerol TEVGs exhibited mechanical properties comparable to NBVs and supported the in vitro proliferation of essential vascular cells—endothelial and smooth muscle cells. After 5 days of culture, the TEVGs exhibited an endothelial monolayer in the lumen, demonstrating their potential for functional vascular tissue regeneration. Our study demonstrates the feasibility of producing TEVGs from SF with tailored mechanical properties, paving the way for more functional and durable TEVGs for future clinical applications.

Mechanical Properties and Economic Analysis of Fused Filament Fabrication Continuous Carbon Fiber Reinforced Composites.

Additive manufacturing of composites offers advantages over metals since composites are lightweight, fatigue and corrosion-resistant, and show high strength and stiffness. This work investigates the tensile and flexural performance of continuous carbon-fiber reinforced (CCF) composites with different guide angles and number of layers. The cost and printing time analyses were also conducted. Tensile specimens with a contour-only specimen and one CCF layer with a 0° guide angle exhibited nearly comparable strength values. Increasing the number of CCF layers enhances the tensile properties. For the identical cost and reinforcement amount, 0°/0° provides a higher tensile strength and elastic modulus compared with 15°/-15°. The same phenomenon was observed for 15°/0°/-15° and 0°/0°/0°. The samples with one and two reinforcement layers had similar stiffness and maximum load values for flexural tests. For the samples with four layers, there was a considerable improvement in stiffness but a minor decrease in the maximum load.

Conceptual design of a prestressed precast UHPC-steel hybrid tower to support a 15 MW offshore wind turbine

Ultra-high-performance-concrete (UHPC) possesses favorable mechanical properties and economy efficiencies and has the potential to be used as the main material to construct tower structures of offshore wind turbines (OWTs). Then the shortages of widely used pure steel OWT towers such as relatively small lateral stiffness, weak fatigue behavior and corrosion resistance can be avoided. In this work, a new type of hybrid tower structure combining a prestressed UHPC tube at the bottom and an upper steel tube is proposed to support a large-size 15 MW OWT. Detailed and carefully refined finite element (FE) analysis by ABAQUS was conducted to explore the dynamic performance of the proposed hybrid tower. The modal properties, dynamic responses, ultimate strength, fatigue strength, and economy performance of the hybrid tower were comprehensively evaluated. It shows that with a similar lateral stiffness but greatly reduced material and maintenance costs compared to a reference steel tower, the hybrid tower has enough ultimate and fatigue strength to resist huge environmental loads caused by extreme winds, waves and earthquakes.

Perturbation approaches to achieving diverse and competitive designs in topology optimisation

Utilising topology optimisation to generate diverse and competitive structures enables designers to create elegant and efficient designs according to their aesthetic intuition and functional needs. This study proposes load and support perturbation approaches based on the bi-directional evolutionary structural optimisation (BESO) method to achieve diverse designs. During the optimisation process, noise is introduced to the sensitivity calculations by randomly perturbing the load angles or material properties near the supports, leading to a variety of local optima. Numerical results demonstrate that the proposed approaches are capable of creating designs with similar stiffness but distinct geometries. In addition, the diversity of the obtained solutions can be controlled by utilising different perturbation functions. This work is of significant practical importance in both architecture and engineering, where multiple design options of high structural performance are in demand.

Visceral pleura mechanics: Characterization of human, pig, and rat lung material properties

Pulmonary air leaks are amongst the most common complications in lung surgery. Lung sealants are applied to the organ surface and need to synchronously stretch with the visceral pleura, the layer of tissue which encompasses the lung parenchymal tissue. These adhesives are commonly tested on pig and rat lungs, but applied to human lungs. However, the unknown mechanics of human lung visceral pleura undermines the clinical translatability of such animal-tested sealants and the absence of how pig and rat lung visceral pleura compare to human tissues is necessary to address. Here we quantify the biaxial planar tensile mechanics of visceral pleura from healthy transplant-eligible and smoker human lungs for the first time, and further compare the material behaviors to pig and rat lung visceral pleura. Initial and final stiffness moduli, maximum stress, low-to-high strain transition, and stress relaxation are analyzed and compared between and within groups, further considering regional and directional dependencies. Visceral pleura tissue from all species behave isotropically, and pig and human visceral pleura exhibits regional heterogeneity (i.e. upper versus lower lobe differences). We find that pig visceral pleura exhibits similar initial stiffness moduli and regional trends compared to human visceral pleura, suggesting pig tissue may serve as a viable animal model candidate for lung sealant testing. The outcomes and mechanical characterization of these scarce tissues enables future development of biomimetic lung sealants for improved surgical applications. Statement of significanceSurgical lung sealants must synchronously deform with the underlying tissue and with each breath to minimize post-operative air leaks, which remain the most frequent complications of pulmonary intervention. These adhesives are often tested on pig and rat lungs, but applied to humans; however, the material properties of human lung visceral pleura were previously unexplored. Here, for the first time, the mechanics of human visceral pleura tissue are investigated, further contrasting rarely acquired donated lungs from healthy and smoking individuals, and additionally, comparing biaxial planar material characterizations to animal models often employed for pulmonary sealant development. This fundamental material characterization addresses key hindrances in the advancement of biomimetic sealants and evaluates the translatability of animal model experiments for clinical applications.

Effect of aliovalent substitution on the local structure of CaKFe4As4 superconductor

We have investigated the local structure of the iron-based CaKFe4As4superconductor featuring distinct aliovalent substitutions at the Ca and K sites, that is CaKFe4As4, CaK0.9Sr0.1Fe4As4, CaK0.9Ba0.1Fe4As4and Ca0.9Na0.1K0.9Ba0.1Fe4As4. Temperature-dependent Fe K-edge extended x-ray absorption fine structure (EXAFS) measurements are used to determine the near-neighbors bondlengths and their stiffness. The EXAFS analysis reveals that the Fe-As bondlength undergoes negligible changes by substitution, however, the Fe-Fe bondlength and the As height are affected by the Sr substitution. The superconducting transition temperatures of CaK0.9Sr0.1Fe4As4and CaK0.9Ba0.1Fe4As4are very similar even if the mean As heights are significantly different suggesting that the anion height may not be a unique parameter to describe the superconductivity in CaKFe4As4. The mean As heights show a peculiar temperature dependence characteristic of CaKFe4As4system. Furthermore, the temperature-dependent mean square relative displacements reveal similar Fe-Fe bond stiffness in all samples, instead the Fe-As bond is substantially stiffer in case of CaK0.9Sr0.1Fe4As4. The local structure results are discussed in relation to the differing transport properties of aliovalent substituted 1144 superconductor.

Experimental and numerical study on cyclic behavior of precast segmental railway bridge columns with lap-spliced rebar connections using UHPC wet joints

A novel lap-spliced connection with ultra-high performance concrete (UHPC) was proposed to connect precast railway bridge column segments with footings or cap beams. Two 1:3 scaled specimens were tested under cyclic loads. Specimen PC-LS was a precast column with lap-spliced rebar connection and UHPC wet joints. Specimen CIP was a monolithic column as a reference specimen. The test results revealed that the plastic hinge region of the precast specimen shafted to the upper surface of the UHPC wet joint. The failure mode of the precast specimen was exhibited as the crushing of the core concrete and the buckling/fracture of the longitudinal rebars. Specimen PC-LS experienced larger lateral force, initial stiffness, comparable ductility, and energy dissipation compared to specimen CIP. Additionally, the validated finite-element (FE) models considering the bond-slip effect between concrete and reinforcements were developed. The parametric studies were carried out to further investigate the cyclic behavior of specimen PC-LS. Analytical results demonstrated that the variation of the compressive and tensile strength of UHPC showed little influence on the cyclic behavior of the precast segmental column, which indicated that UHPC with natural curing and lower volume content steel fibers could be used as the wet joint. The precast segmental column had similar lateral force and stiffness with the cast-in-place column when the lap splice length was 10 times of the rebar diameter.

Design optimization of lightweight automotive seatback through additive manufacturing compression overmolding of metal polymer composites

With the growing demand for enhanced automotive fuel efficiency and environmental sustainability, there is a need for lightweighting automotive components through innovative design and manufacturing processes. This study leverages a combination of numerical iterative design optimization and hybrid additive manufacturing–compression molding (AM-CM) technique for metal polymer composites to lightweight an automotive seatback. The AM-CM process enables robust mechanical interlocking between metals and composites, boasting high stiffness and strength with low overall density. Replacing metallic components with such metal polymer composites allows for comparable mechanical performance while significantly reducing the overall weight. First, the automotive seatback design space is reduced to critical load carrying regions using topology optimization and high stress concentration areas are identified using finite element analysis. Next, a lightweight metal polymer subcomponent is designed for a high stress concentration region. The full seatback frame with spatially heterogeneous material-specific design is then iteratively optimized to enable enhanced stiffness with minimal weight. Overall, the automotive seatback frame designed with location-specific metal, polymer, and metal polymer composite materials weighs 20% less than the metal-only design while exhibiting similar stiffness.

Low-Load Resistance Exercise With Blood Flow Restriction Versus High-Load Resistance Exercise on Hamstring Muscle Adaptations in Recreationally Trained Men.

Kamiş, O, Gürses, VV, Şendur, HN, Altunsoy, M, Pekel, HA, Yıldırım, E, and Aydos, L. Low-load resistance exercise with blood flow restriction vs. high-load resistance exercise on hamstring muscle adaptations in recreationally trained men. J Strength Cond Res XX(X): 000-000, 2024-Low-load resistance exercise with blood flow restriction (BFR-RE) has been suggested as a viable alternative exercise for traditional high-load resistance exercise (HL-RE). However, very little is known about hamstring muscle thickness, stiffness, and strength after BFR-RE. This study aimed to compare the effects of 6 weeks of BFR-RE and HL-RE on hamstring muscle thickness, stiffness, and strength. Twenty-nine recreationally trained men were pair matched and randomly assigned to the BFR-RE (n = 15) and HL-RE (n = 14) groups. The BFR-RE groups performed bilateral lying leg curl exercise (30-15-15-15 reps, 30-second rest between the sets, 30% 1RM) with BFR cuffs (60% of limb occlusion pressure), whereas HL-RE performed the same exercise (3 × 12 reps, 90-second rest between the sets, 70% 1RM) without BFR cuffs. Hamstring muscle thickness, muscle stiffness, isokinetic muscle strength, and 1RM were assessed at baseline and follow-up after completing a 6-week resistance exercise program (3× a week). Hamstring muscle thickness was assessed by ultrasonography, whereas muscle stiffness was evaluated by shear wave elastography. Isokinetic dynamometry and a 1RM strength test were used to determine muscular strength before and after the exercise program. Statistical significance was set at p < 0.05. No significant effect was found for hamstring muscle strength, thickness, and stiffness for group and group × time interaction, and only a significant main effect of time (p < 0.001) was observed for all outcomes. Both groups experienced significant improvements for all outcomes from baseline to follow-up without any between-group differences. In conclusion, results revealed that BFR-RE can provide similar hamstring muscle strength, thickness, and stiffness compared with HL-RE.

Preheating of Gelatin Improves its Printability with Transglutaminase in Direct Ink Writing 3D Printing.

Gelatin and transglutaminase (TG) ink is increasingly popular in direct ink writing three-dimensional (3D) printing of cellular scaffolds and edible materials. The use of enzymes to crosslink gelatin chains removes the needs for toxic crosslinkers and bypasses undesired side reactions due to the specificity of the enzymes. However, their application in 3D printing remains challenging primarily due to the rapid crosslinking that leads to the short duration of printable time. In this work, we propose the use of gelatin preheated for 7 days to extend the duration of the printing time of the gelatin ink. We first determined the stiffness of freshly prepared gelatin (FG) and preheated gelatin (PG) (5 – 20% w/w) containing 5% w/w TG. We selected gelatin hydrogels made from 7.5% w/w FG and 10% w/w PG that yielded similar stiffness for subsequent studies to determine the duration of the printable time. PG inks exhibited longer time required for gelation and a smaller increase in viscosity with time than FG inks of similar stiffness. Our study suggested the advantage to preheat gelatin to enhance the printability of the ink, which is essential for extrusion-based bioprinting and food printing.

Metabolic Profiles of Encapsulated Chondrocytes Exposed to Short-Term Simulated Microgravity.

The mechanism by which chondrocytes respond to reduced mechanical loading environments and the subsequent risk of developing osteoarthritis remains unclear. This is of particular concern for astronauts. In space the reduced joint loading forces during prolonged microgravity (10-6 g) exposure could lead to osteoarthritis (OA), compromising quality of life post-spaceflight. In this study, we encapsulated human chondrocytes in an agarose gel of similar stiffness to the pericellular matrix to mimic the cartilage microenvironment. We then exposed agarose-chondrocyte constructs to simulated microgravity (SM) using a rotating wall vessel (RWV) bioreactor to better assess the cartilage health risks associated with spaceflight. Global metabolomic profiling detected a total of 1205 metabolite features across all samples, with 497 significant metabolite features identified by ANOVA (FDR-corrected p-value < 0.05). Specific metabolic shifts detected in response to SM exposure resulted in clusters of co-regulated metabolites, as well as key metabolites identified by variable importance in projection scores. Microgravity-induced metabolic shifts in gel constructs and media were indicative of protein synthesis, energy metabolism, nucleotide metabolism, and oxidative catabolism. The microgravity associated-metabolic shifts were consistent with early osteoarthritic metabolomic profiles in human synovial fluid, which suggests that even short-term exposure to microgravity (or other reduced mechanical loading environments) may lead to the development of OA.

Titanium-Coated Polyetheretherketone Cages Vs Full Titanium Cages for Stand-Alone 1- or 2-Level Anterior Cervical Discectomy and Fusion: A Comparative Study.

Anterior cervical discectomy and fusion (ACDF) for the surgical treatment of cervical degenerative disease often includes an intervertebral cage, which restores disc height and lordosis while promoting fusion . Cage materials include titanium (TTN) or polyetheretherketone (PEEK). Controversy in material selection stems from higher fusion rates with TNN, despite a higher subsidence rate, while PEEK cages demonstrate superior preservation of interspace height. Combining the advantages of both materials, TTN-coated PEEK (TCPEEK) cages were developed, featuring a PEEK core with similar stiffness to the bone, enveloped with a TTN coat, improving osteointegration. However, the potential superiority of TCPEEK over TTN cages has not been investigated. This study aimed to compare clinical and radiographic outcomes following single- or double-level ACDF using either TTN or TCPEEK cages. This retrospective single-center study included patients undergoing single- or double-level ACDF between 2017 and 2019. Clinical outcomes included the Neck Disability Index and revision surgery incidence. Radiographic parameters included cervical and segmental lordosis, C2 to C7 sagittal vertical axis, fusion, subsidence, and adjacent segment degeneration at a minimum 12-month follow-up. A total of 45 patients (16 TTN; 29 TCPEEK) and 58 cervical levels (21 TTN; 37 TCPEEK) were included. Both cages significantly improved Neck Disability Index scores (TTN -10.0; TCPEEK -14.1) without significant differences. Two single-level TCPEEK patients required revision surgery due to non-union. In the radiological assessments, no significant difference was found for subsidence rates (TTN 52.4%; TCPEEK 56.8%), adjacent segment degeneration, cervical and segmental lordosis, and changes in C2 to C7 sagittal vertical axis. Though not statistically significant, fusion rates trended slightly higher with TTN (90.5%) vs TCPEEK cages (86.5%). TTN and TCPEEK cages achieve satisfactory clinical and radiological outcomes in single- or double-level ACDF. This finding suggests that the choice between them can be based on other factors, such as surgeon preference or availability, rather than specific material properties. This study found that the selection of ACDF cage material did not affect clinical outcomes.

A comparison of static and rotordynamic characteristics for two types of liquid annular seals with and without helical grooved stator

Less leakage is a benefit of parallel grooved liquid seals (labyrinth seals). But researches show that the liquid seal with parallel grooves on the rotor harms the rotor stability. The seal with helical grooves on stator performs well in terms of rotordynamics, and its leakage is sensitive to the rotating speed. To make use of the advantages of both seals and improve seal stability, based on the Smooth-stator/Parallel Grooved-rotor (SPG) liquid seal, a Helical Grooved-stator/Parallel Grooved-rotor (HGPG) liquid seal is designed. To evaluate two liquid seals’ leakage, rotordynamic characteristics and drag power loss, a transient computational fluid dynamics-based method is employed. This method is based on the multi-frequency elliptical-orbit rotor whirling model and the mesh deformation technique. The published experimental data of the leakage and rotordynamic force coefficients for an SPG liquid seal are used to validate the accuracy and dependability of the current method. Seal leakage and force coefficients are presented and compared for the SPG liquid seal and the HGPG liquid seal at various pressure drops. The influences of parallel groove depth on the leakage and rotordynamic properties for the HGPG liquid seals at two rotational speeds (2000, 6000 r/min) are analyzed. The numerical findings demonstrate that the novel HGPG liquid seal has a lower leakage flow rate (by ∼22.3%) than the traditional SPG liquid seal. There is an optimal parallel groove depth that minimizes leakage. The presented novel HGPG liquid seal significantly improves rotordynamic stability, due to the similar effective stiffness and the obviously larger positive effective damping. Reducing parallel groove depth can increase the positive effective damping. In terms of leakage and rotordynamic characteristics, the novel HGPG liquid seal is a better seal design for liquid turbomachinery.

Damaged mortise–tenon joint strengthened using an assembled spring–steel hoop based on a design of similar rotational stiffness

The excessive enhancement of rotational stiffness leads to an uneven distribution of rotational stiffness across the entire timber frame. This uneven distribution concentrates the load effect on the strengthened mortise–tenon joints (MJs), causing localized failure in advance. To address this issue, a reversible and non-destructive strengthening method for the damaged MJs is devised, involving the utilization of an assembled spring-steel hoop. The rotational stiffness of a strengthened MJ is derived from the springs that link the steel hoop of the beam and column, resembling the rotational stiffness of an intact MJ. A comparison of the measured moment-rotation curves of various types of MJ reveals that the rotational stiffness similarity necessitates the strengthened MJ's yield moment and ultimate moment, along with the associated rotation angles, to closely resemble those of the intact MJ. Secondly, a design methodology is developed for the assembled spring-steel hoop, which involves determining the parameters of the connecting spring. The comparative analysis of the hysteretic test results on multiple strengthened and intact MJs indicates that the strengthened MJs exhibit rotational stiffness deviations of less than 12.2 % during the elasticity stage and 6.6 % during the plasticity stage, in comparison to the intact MJs. A spring exhibits a lower ultimate extension compared to timber, leading to a maximum discrepancy of 37.5 % in the ductility coefficients between the strengthened MJs and intact MJs. The strengthened and intact MJs display similar patterns of stiffness reduction and energy dissipation progression.

Influence of Platen Stiffness on the Contact Stress Distribution in the Standardized Uniaxial Compression Test

Uniaxial compressive strength is an essential mechanical parameter to adequately characterize any given material. Numerous standards have been developed to guarantee reliable testing execution, as well as the repeatability of results. In this sense, not only the geometric dimensions and tolerances of both the platen and the specimen have been prescribed, but also the testing parameters, such as the load application speed. However, all these recommendations are based on the assumption that the stresses are uniformly distributed across the contact interface between the platen and the specimen. Nevertheless, this is major elastic simplification that allows for obtaining a handy and useful formula to determine the compressive strength, but this strongly deviates the theoretical foundations from the actual experimental reality. Experimental and numerical research to determine the influence of relative stiffness between the specimen and the platen on the stress distribution generated during the execution of the uniaxial compressive test is performed. The results prove that the stresses are not uniformly distributed across the contact when the platen material is significantly stiffer or softer (less stiff) than that of the tested specimen, and additionally, an undesired triaxial stress field is induced inside the specimen. For these reasons, the use of platens with a similar stiffness to that of the specimen is strongly recommended, as it allows for the uniform distribution of the compressive contact stresses and minimizes the influence of the triaxial stress field.

Experimental study on the vibrational response and damping of short fibre/unidirectional PEEK hybrid composites

This study investigates the vibrational performance of a new generation of hybrid composites based on a short fibre core for structural dynamic applications. The dynamic characterisation was conducted through dynamic mechanical analysis (DMA) and the free vibration of a cantilever beam at different lengths. The short fibre composite exhibited a superior specific damping capacity, producing a maximum damping of 0.42. The values extracted from DMA indicated no visco-plastic contribution from the PEEK resin system, implying that the internal microarchitecture drives the material damping, with friction at the fibre/matrix interface being the primary dissipation mechanism. The hybrid laminate presented an improvement in damping performance of 39% due to the addition of the short fibre core compared to a baseline quasi-isotropic laminate of similar flexural stiffness. These findings show hybridisation’s advantages in designing structural components with improved damping performance and reduced cost for a variety of dynamic applications in industries such as automotive.

3D bioartificial stretchable scaffolds mimicking the mechanical hallmarks of human cardiac fibrotic tissue.

Human cardiac fibrotic tissues are characterized by a higher stiffness relative to healthy cardiac tissues. These tissues are unable to spontaneously contract and are subjected to passive mechanical stimulation during heart functionality. Moreover, scaffolds that can recapitulate the in vivo mechanical properties of the cardiac fibrotic tissues are lacking. Herein, this study aimed to design and fabricate mechanically stretchable bioartificial scaffolds with biomimetic composition and stiffness comparable to human cardiac fibrotic tissues. Poly(ε-caprolactone) (PCL) scaffolds with a stretchable mesh architecture were initially designed through structural and finite element method (FEM) analyses and subsequently fabricated by melt extrusion additive manufacturing (MEX). Scaffolds were surface functionalized by 3,4-dihydroxy-DL-phenylalanine (DOPA) polymerization (polyDOPA) to improve their interaction with natural polymers. Scaffold pores were then filled with different concentrations (5%, 7%, and 10% w/v) of gelatin methacryloyl (GelMA) hydrogels to support in vitro human cardiac fibroblasts (HCFs) 3D culture, thereby producing bioartificial PCL/GelMA scaffolds. Uniaxial tensile mechanical tests in static and dynamic conditions (1 Hz; 22% maximum strain) demonstrated that the bioartificial scaffolds had in vivo-like stretchability and similar stiffness to that of pathological cardiac tissue (tailored as a function of the number of PCL scaffold layers and GelMA hydrogel concentration). In vitro cell tests on bioartificial scaffolds using HCF-embedded GelMA hydrogels under static conditions displayed increasing cell viability, spreading, and cytoskeleton organization with decreasing GelMA hydrogel concentration. Moreover, α-smooth muscle actin (α-SMA)-positive cells were detected after 7 days of culture in static conditions followed by another 7 days of culture in dynamic conditions and not in HCF-loaded scaffolds cultured in static conditions for 14 days. These results suggested that in vitro culture under cyclic mechanical stimulations could induce an HCF phenotypic switch into myofibroblasts.

Experimental Study on the Seismic Performance of Insulated Single-Sided Composite Shear Walls under Different Shear Spans and Axial Compression Ratios

The new insulated single-sided composite shear wall (NISCSW) composition involves setting a precast wall panel on one side and an insulation panel on the other side, with a middle cavity for casting concrete. To investigate the seismic performance of NISCSW under different shear spans and axial compression ratios, eight specimens are made, including six composite and two cast-in-place walls. The shear span ratio is controlled at 1.2 and 1.9, and the axial compression ratio is controlled at 0.1, 0.3, and 0.4. The specimens are subjected to quasistatic tests to analyze failure modes, hysteresis characteristics, stiffness degradation, displacement ductility, and energy dissipation capacity and to compare the seismic performance of the composite and cast-in-place walls. Results show that for each composite specimen, under the same axial compression ratio, the large shear span ratio specimen has a lower ultimate bearing capacity and faster stiffness degradation but better ductility and postyield energy dissipation capacity. Under the same shear span ratio, the high axial compression ratio specimen had a higher ultimate bearing capacity, slightly worse ductility, and similar stiffness degradation and energy dissipation capacity compared to other specimens. Compared with the cast-in-place specimen with the same axial compression ratio, the composite specimen failure mode and hysteresis characteristics are similar, and the ductility and energy dissipation capacity are comparable to the cast-in-place shear wall specimen, indicating that NISCSW has similar seismic performance to the cast-in-place shear wall under conditions of a large shear span ratio and high axial compression ratio. Based on the test results, the program ABAQUS is used to simulate the specimens. Compared with the test results, the simulated specimen failure mode is consistent with the test results, and the hysteresis and skeleton curves are consistent with the test curve, indicating that the model is correct, reliable, and can be verified with test results.