Concentration Of Silver Nanoparticles Research Articles

Enhanced Sensing Performance of an Ammonia Gas Sensor Based on Ag‐Decorated ZnO Nanorods / Polyaniline Nanocomposite

AbstractThe development of low‐cost ammonia sensors with high sensitivity and selectivity has gained considerable interest. Though the response of these sensors at room temperature is low and needs enhancement. In the present study high sensitivity ammonia gas sensors based on nanocomposite films of polyaniline (PANI) and with varying ZnO concentrations were synthesized and investigated. With a loading of 10 at% ZnO, the gas sensing response of 59 % was obtained for 120 ppm NH3 gas. The gas response was further enhanced by decorating the ZnO nanorods with different concentrations of silver (Ag) nanoparticles. The Ag‐decorated ZnO nanorods were embedded in the PANi matrix using the in‐situ oxidative polymerization technique. It was shown that PANi ZnO, p‐n junction, and the introduction of porosity in nanocomposite act synergistically in increasing the resistance caused by the deprotonation of PANi by NH3. Among various compositions studied, 2 % loading of Ag in ZnO embedded in PANi matrix, thin films were found to be highly selective and sensitive towards NH3 gas at room temperature with a chemiresistive response of 70 % at 120 ppm and a recovery time of less than 120 s. The selectivity of the nanocomposite was also studied towards various reducing and oxidizing gasses.

The Mechanism of Phase Transfer Synthesis of Silver Nanoparticles Using a Fatty Amine as Extractant/Phase Transfer Agent

The paper presents the research results on synthesizing silver nanoparticles in aqueous solutions and their extraction into the organic phase. Studies have shown that it is best to perform the extraction process using n-hexane > cyclohexane > toluene > chloroform > ethyl acetate. The results show a correlation between the dielectric constant of the organic phase and its ability to extract nanoparticles. The lower the dielectric constant is, the higher the extractability. The hydrodynamic radius of the silver nanoparticles changes after transfer to the organic phase, depending greatly on the organic phase used. The extraction mechanism is complex and multi-step. As the first step, the Ag nanoparticles are transferred to the phase boundary. As the second step, the octadecylamine (ODA) molecules adsorb on the silver nanoparticles (AgNPs) surface. The change in particle shape was also noted. This suggests that the interfacial processes are more complex than previously reported. Below the initial concentration of ODA 2 × 10−4 M, the formation of a third phase has been observed. In a one-stage experiment, the concentration of silver nanoparticles after transferring to the organic phase was increased 500 times in about 10 s. The role of the concentration of ODA, therefore, is not only a measure of the extraction efficiency and productivity but functions as an enabler to maintain favorable biphasic processing, which underlines the role of the solvent again.

Activation of silver nanoparticles by hydrophilic encapsulation for antimicrobial powder coated surfaces

Silver nanoparticles (AgNP) are known as an antimicrobial agent, but they cannot be efficiently converted to active silver ions due to the lack of hydrophilic reaction environment when incorporated into a dry powder coating film. To make these agents effective in the powder coated surface, a novel encapsulation approach was proposed. Silver nanoparticles were first synthesized on the surface of synthetic zeolite A by in-situ chemical reduction method, which was followed by the addition of copper ions through ion exchange. The functionalized zeolite was then encapsulated with different water-soluble hydrophilic polymers to facilitate the generation of silver ions from nanoparticles. The encapsulated additives were incorporated into the resin system for making the antimicrobial powder coating film. The properties of the additives and films were carefully analyzed, and results show that appropriate loading of silver guarantees a high concentration and uniform distribution of silver nanoparticles on the zeolite surface. The strength of the encapsulation with the additive is found to depend on the cations present in the additives. Anionic polymer encapsulation is the most effective solution for releasing silver ions from antimicrobial coated surface. Excessive hydrophilicity is not beneficial to antimicrobial ability and brings cracks on the surface. Additives with non-polymeric hydrophilic encapsulation made from the precipitation of calcium nitrate and sodium phosphate also shows acceptable antimicrobial efficiency. The proposed hydrophilic encapsulation method can well facilitate the activation of silver nanoparticles and ensure an effective antimicrobial property of the powder coating film.

The Synthesis AgNPs of Leaves Melastoma malabathricum Extract reduce Adult Haemonchus contortus Motility Assay

The research was performed to observe the effectiveness of silver nanoparticles (AgNPs) incorporate using the leaves Melastoma malabathricumin the ethanolic extract as anti-Haemonchus contortus. To determine the phytochemical content of Melastoma malabathricum, a qualitative phytochemical test was done. An adulticidal or an adult motility test was performed on mature female Haemonchus contortus. Three silver nanoparticle concentrations (AgNPs-Extract 0.2mgml-1, AgNPs-Extract 0.1mgml-1, AgNPs-Extract 0.05mgml-1) and two different ethanol extract Melastoma malabathricum concentrations (200mgml-1 and 100mgml-1) were examined. Albendazole 40mg/ml was used asa standard reference, and for negative control normal using saline water. The phytochemical analysis revealed that the ethanol extract of Melastoma malabathricum contains tannin, flavonoid, terpenoid, and steroid. The silver nanoparticles are identified visually as colour changes in the solution and using an ultraviolet-visible nanophotometer (UV-Vis). The spectrum was examined using a UV-Vis nanophotometer, and it revealed a clear peak around 365nm with a pH of 5.9. In a nematocidal assay on adult Haemonchus contortus, the paralysis time of the worm in silver nanoparticle (AgNPs- Extract 0.2mg/ml) was 8.85 minutes, which was significantly (P0.05) shorter than the control positive (14.07 minutes). Worms died in less than an hour in all treatments, and the negative control worm remained active for up to 2 hours after exposure

A New Detection Method of Oral and Oropharyngeal Squamous Cell Carcinoma Based on Multivariate Analysis of Surface Enhanced Raman Spectra of Salivary Exosomes.

Raman spectroscopy recently proved a tremendous capacity to identify disease-specific markers in various (bio)samples being a non-invasive, rapid, and reliable method for cancer detection. In this study, we first aimed to record vibrational spectra of salivary exosomes isolated from oral and oropharyngeal squamous cell carcinoma patients and healthy controls using surface enhancement Raman spectroscopy (SERS). Then, we assessed this method's capacity to discriminate between malignant and non-malignant samples by means of principal component-linear discriminant analysis (PC-LDA) and we used area under the receiver operating characteristics with illustration as the area under the curve to measure the power of salivary exosomes SERS spectra analysis to identify cancer presence. The vibrational spectra were collected on a solid plasmonic substrate developed in our group, synthesized using tangential flow filtered and concentrated silver nanoparticles, capable of generating very reproducible spectra for a whole range of bioanalytes. SERS examination identified interesting variations in the vibrational bands assigned to thiocyanate, proteins, and nucleic acids between the saliva of cancer and control groups. Chemometric analysis indicated discrimination sensitivity between the two groups up to 79.3%. The sensitivity is influenced by the spectral interval used for the multivariate analysis, being lower (75.9%) when the full-range spectra were used.

Influence of silver nanoparticles in the ion-exchange layer of photo-thermo-refractive porous glass on the spectral-luminescent properties of CsPbBr3 perovskite nanocrystals

The spectral-luminescent properties of new composite materials based on porous matrices of photo-thermo-refractive glasses with a silver ion-exchange layer and CsPbBr3 inorganic perovskite nanocrystals have been studied. Perovskite nanocrystals were introduced into a porous matrix by the method of pumping solutions through a sample under piston pressure. Two mechanisms of interaction between silver nanoparticles and perovskite nanocrystals have been revealed, such as enhancement of the local field and luminescence quenching due to the high absorption of nanoparticles. The quantum yield of the studied samples under different irradiation associated with an increase in the concentration of silver nanoparticles was 34% without irradiation, 51% with 100 j irradiation dose, and 25% with 1000 j irradiation dose. In this case, an increase in the integrated luminescence intensity was observed in glasses irradiated with 100 J by a factor of 2.97 and irradiated with 1000 J by a factor of 1.76 compared to the unirradiated sample. The average luminescence lifetime decreases from 2.19 to 1.68 ns with an increase in the concentration of nanoparticles.

Growth of New, Optically Active, Semi-Organic Single Crystals Glycine-Copper Sulphate Doped by Silver Nanoparticles

The purpose of this study is to modify all physicochemical properties of glycine–copper sulphate single crystals, such as crystal habits, molar mass, thermal stability, optical activity, and electrical properties. The novelty of this study is growth of glycine–copper sulphate single crystals doped by a low concentration of silver nanoparticles (SNPs) that improved both crystal habits and physicochemical properties. The originality of this work is that trace amounts of SNPs largely increased the crystal size. Crystals have molar stoichiometric formula [glycine]0.95, [CuSO4·5H2O]0.05 in the absence and presence of silver nanoparticles (SNPs) in different concentrations: 10 ppm, 20 ppm, and 30 ppm. The crystals’ names and abbreviations are: glycine–copper sulphate (GCS), glycine–copper sulphate doped by 10 ppm SNPs (GCSN1), glycine–copper sulphate doped by 20 ppm SNPs (GCSN2), and glycine–copper sulphate doped by 30 ppm SNPs (GCSN3). Dopant silver nanoparticles increased: crystallinity reflecting purity, transparency to UV-Vis. electromagnetic radiation, thermal stability, and melting point of glycine–copper sulphate single crystal. GCSN3 is a super conductor. High thermal conductivity of crystals ranging from 1.1 W·min−1·K−1 to 1.6 W·min−1·K−1 enabled attenuation of electromagnetic radiation and rapid heat dissipation due to good dielectric and polar properties. On rising temperature, AC electrical conductivity and dielectric properties of perfect crystal GCSN3 increased confirmed attenuation of thermal infrared radiation.

Development and Experimental Evaluation of Some Silver Nanoparticles with Antimicrobial Potential

By adjusting the synthesis process, silver nanoparticles (AgNp) of various shapes, sizes, and structures can be obtained, all of which have a substantial impact on the biological effect, notably, the regulation of antibacterial activity in the present circumstances of growing bacterial resistance. Due to their relatively small size, nanoparticles may be disseminated evenly throughout the body of the experimental animal, even at low doses, and exert more potent antibacterial activities. Our research was centered on the synthesis, production, and biological evaluation of antibacterial silver nanoparticles. Using the Turkevich method, we were able to effectively synthesize and characterize nanoscale silver particles, with an average crystallite size of 9.49 nm. We examined their acute toxicity and pharmacokinetic characteristics in rats after administering a single dosage. In addition, we evaluated the biological effect of topical AgNp suspension on the progression of burn-type lesions in the experimental animals. The pharmacokinetic profile demonstrated that the plasma concentration of silver nanoparticles, as well as their clearance rate, and dispersion throughout the body, are significantly enhanced in large rodent species. The restorative effect of synthesized silver nanoparticles in the form of a suspension in distilled water was corroborated by the values of the hematological parameters. These results demonstrated an intense stimulation of the cellular and molecular processes of the local immune defense, which has resulted in significantly faster regeneration in the AgNp-treated group.

Fabrication and characterization of silver-substituted bioactiveglass incorporated with sodium alginate and graphene oxide

The temporary scaffold is fabricated as an adherent material for the implanted cells and physical support to direct the construction of the substitute tissues, with have high porous surface, large surface/volume ratio, high mechanical features, biocompatible, and biodegradable properties. This study aims to prepare a scaffold of bioactive glass(BG)/sodium alginate(SA)/graphene oxide(GO) composite doped with different concentrations of silver (Ag) nanoparticles with a sol-gel method. The obtained composites were evaluated by a variety of techniques (TGA, DSC, FTIR, XRD, SEM, and EDXA). Through an invitro test using simulated body fluid (SBF), the bioactivity of each specimen was investigated. Gram-positive bacteria were investigated for antimicrobial activity utilizing the agar diffusion method, such as (Staphylococcus aureus and Bacillus subtilis), gram-negative bacteria including (Pseudomonas aeruginosa and Escherichia coli), and fungi (Candida albicans). The bioactivity findings revealed that the surface of the prepared scaffold had formed a layer of hydroxyapatite (HA) as the amount of Ag was raised to (2%). This layer grew but decreased in (3%) and (4%) of Ag concentration, which assures the bioactivity of the prepared samples. The anti-microbial results exhibited a clearing inhibition zone around the samples which confirms the influence of silver doped composites on the proliferation of bacteria and fungi.

Cytotoxic effect of silver and oxide graphene nanoparticles on aberrant forms of Escherichia coli

The acquisition of aberrant morphological variations by microorganisms can serve as a phenotypic manifestation of antibiotic resistance, which results from ad-aptation to growth in a potentially toxic environment. Therefore, morphogenesis should be considered as an adaptive process that makes a great contribution to the ubiquity and universality of prokaryotes. The aim of the research was to evaluate the antibacterial activity of silver and oxidized graphene nanoparticles on pheno-typically aberrant forms of Escherichia coli. As a result of research, it was found that the antibacterial activity of silver nanoparticles is significantly higher compared to that of oxidized graphene nanoparticles on pleomorphic varieties of Escherichia coli, as the minimum inhibitory concentration of silver nanoparticles is lower (13.1 µg/ml) than that of oxidized graphene nanoparticles (75.3-94.9 µg/ml). The mor-phological variance of E. coli correlated with the acquisition of antibiotic resistance by the bacterium, but was not accompanied by a simultaneous increase in resistance to the nanoparticles. According to the results of atomic force microscopy, the toxic effect of oxidized graphene nanoparticles at supratoxic concentrations on aberrant forms of E. coli did not differ from that on canonical forms of the bacterium and was expressed in the destruction of the bacterial population and filling the intercel-lular space with biomass of degraded bacterial cells.

Получение тонкопленочного покрытия композиционного материала на основе диоксида титана и наночастиц серебра золь-гель методом на стеклянных подложках

A method is presented for obtaining thin-film mesoporous coatings for protective glasses in nano- and optoelectronic devices for various purposes, including: obtaining titanium oxide sols with the addition of silver nitrate, applying coatings by the “dipcoating” method with further heat treatment. The kinetic constants of Smoluchowski's fast and slow coagulation and the activation energy of the process at various concentrations of silver nitrate were calculated. The optimal concentration of silver nanoparticles in the ash was determined by the least squares method.

Utilizing an Automated SERS-Digital Microfluidic System for High-Throughput Detection of Explosives.

The surface-enhanced Raman scattering (SERS) technique is a promising method for the detection of explosives such as 2,4,6-trinitrotoluene (TNT) and 3-nitro-1,2,4-triazol-5-one (NTO) because of its high sensitivity to trace substances. However, most SERS detection processes are often nonautomated as well as exhibit low efficiency and toxic exposure, which often poses potential danger to operators. Herein, we propose the integration of SERS with digital microfluidics (SERS-DMF) for automated, high-throughput, and high-sensitivity detection of explosives. First, we carefully designed a DMF chip comprising 40 drive electrodes and 8 storage electrodes to achieve a high-throughput process. And different concentrations of target molecules, silver nanoparticles (Ag NPs), and salts were loaded into the DMF chip. Then, the droplet aggregation, incubation, and detection processes were automatically controlled using the SERS-DMF platform. In addition, Ag NPs were efficiently aggregated by screening different types and concentrations of salts, resulting in "hotspots" and the SERS effect. With the help of the SERS-DMF platform, two explosive samples were automatically detected with high throughput and high sensitivity. The detection limits of TNT and NTO were 10-7 and 10-8 M, respectively. In addition, compared with nonautomatic operations, the SERS-DMF platform exhibited better reproducibility and higher efficiency for the detection of explosives. The proposed SERS-DMF thus has considerable potential as an analytical technique for detecting hazardous substances.

Wound Healing Activity of Cotton Fabrics Loaded with Silver Nanoparticles in Experimental Model of Diabetes

Diabetes is connected with diminished wound healing, that makes patients liable to continuing difficult wounds. Metal nanomaterials as single conjugates have established to keep possible properties of wound when metal nanoparticles are coupled with other wound covering materials. This study aimed to investigate a possible role of cotton fabrics full with silver nanoparticles (AgNPs) to enhance wound healing in diabetic model induced by streptozotocin (STZ).Animals were classified into four groups including the wounded group that were equivalently covered with sterile dressing that made of cotton fabric which had been saturated with different concentrations of silver nanoparticles, and the control group that was preserved with only cotton covering without any treatment (blank group); percent of wound contraction in different studied groups was estimated. Plasma nitric oxide (NO), malodialdehyde (MDA), reduced glutathione (GSH) were measured. Serum neutrophil elastase and nuclear factor kappa b (NF-κb) were assayed by ELISA. Homocystiene (Hcy) was estimated by HPLC. Our results revealed an elevation in wound area, MDA, NF-κb, Hcy, and elastase in the wound group compared to treated groups concomitant with a decrease in plasma nitric oxide and reduced glutathione activities, while treatment with AgNPs significantly ameliorated these parameters in treated group compared to blank group. AgNPs showed high wound contraction rates according to their used concentration .In conclusion; AgNPs have gained considerable attention amongst researchers in wound healing applications, owing to their physicochemical and biological properties. AgNPs promote wound healing and effectively control the growth of microorganisms at the wound site, and this strategy plays an important role in the treatment of wounds.

Evaluation of In Vitro and In Vivo Antifungal Activity of Green Synthesized Silver Nanoparticles against Early Blight in Tomato

Silver nanoparticles have gained considerable interest in recent decades due to their antimicrobial activity and are used in water disinfection, wound healing, food packaging, and plant protection. This study tested the potential of silver nanoparticles synthesized using the neem (Azadirachta indica) leaf extract against Alternaria solani causes early blight disease in tomato plants. The pathogen was isolated from infected tomato plants and identified using morphological and molecular features. The results showed significant variation among isolates. Isolates, Shk-1 and Ksr-1 were highly pathogenic, causing up to 80% disease incidence. The potential of silver nanoparticles against each isolate was determined using different concentrations of silver nanoparticles. During in vitro and in vivo experiments, the growth inhibition rate of the pathogen was 70–100% at 50 ppm. Lower concentrations of silver nanoparticles (5 and 10 ppm) increased phenolics, PO, PPO, and PAL production by more than 50% as compared to the untreated control. These defensive mechanisms clearly demonstrate the fungicidal potential of AgNPs and recommend their utilization in different crop protection programs.

Green synthesized silver nanoparticles from eucalyptus leaves can enhance shelf life of banana without penetrating in pulp.

Bananas are exposed to serious post-harvest problems resulting in agricultural and economic losses across the world. The severity of problem is linked with the process of rapid ripening and pathogens attack. Such problems have led to economic losses as well as a lower yield of nutritionally rich bananas. The global demand to increase the life span of bananas and their protection from pathogens-borne diseases urged the use of antimicrobial edible coatings of nanoparticles. The present experiment has explored the innovative development of green synthesized nanoparticles from Eucalyptus leaf extract (ELE) to increase the shelf life of bananas up to 32 days from the day of collection. Statistically significant results were recorded (P = 0.05) by applying five different concentrations of silver nanoparticles (AgNPs) in ranges of 0.01-0.05%. Various morphological and physiological parameters such as color, decay, firmness, weight loss, pulp to peel ratio, pH, titrable acidity (TA), phenolic contents, protein estimation, ethylene production, starch content and total soluble sugars were measured in Cavendish banana (Basrai). Bananas treated with 0.01% AgNPs showed maximum control on its ripeness over morphological and physiological changes. The increase in shelf life was in order 0.01%>0.02%>0.03%>0.04%>0.05%> control. Further, AgNPs reduced the process of ripening by controlling ethylene production. The result has also proved the safety of banana consumption by simple removal of banana peel as penetration of AgNPs from the peel to the pulp was not detected. It is recommended to use 0.01% AgNPs to enhance the shelf life of banana without effecting its nutritive value.

DEVELOPMENT OF TECHNOLOGY OF PRODUCTION AND QUALITY ASSESSMENT OF DRUG SUBSTANCE " DRY EXTRACT OF SCUTELLARIA ISCANDERI L. WITH SILVER NANOPARTICLES"

This study demonstrates the results of experimental studies aimed at the development of technology and assessment of quality of drug substance containing a dry extract of a flavonoid-containing herb and silver nanoparticles, as well as the optimal technological parameters of the phytosynthesis process and methods for the qualitative and quantitative content of biologically active substances have been developed. Phytosynthesis of silver nanoparticles was carried out using plant extracts, in which biologically active substances, flavonoids, act as an agent that reduces silver ions to nanoparticles. The optimal technology for the production of silver nanoparticles was chosen and the sample obtained at t =50 °C and the ratio of extract: silver nitrate = 1:4 was chosen as the optimal one. Additionally, the expiration date of the drug was determined for the development of regulatory documentation. During quality assessment, it was found that the quantitative content of silver nanoparticles should be at least 2 mg/g. As a result, it became possible to produce a substance used in the preparation of local antibacterial and antifungal drugs for the treatment of skin diseases.

Biological activity of synthesized silver nanoparticles on S. aureus and P. aeruginosa

Silver nanoparticles (AgNPs) that were biologically synthesized using Moringa oleifera leave extract showed good antibacterial activity against two tested bacterial strains namely: Pseudomonas aeruginosa as a model for Gram- negative bacteria and Staphylococcus aureus as a model for Gram- positive bacteria. Minimum inhibitory concentration (MIC) of antibacterial silver nanoparticles that were biologically synthesized using Moringa oleifera leave extract was tested to estimate the effect of nanoparticles on cell viability, cell integrity, and cell permeability of tested bacteria. The results indicated that there was a great effect of AgNPs on cell viability of bacterial strains tested as there was significance in growth kinetics between control and treated bacteria with silver nanoparticles. Also, cell permeability of S. aureus and P. aeruginosa were greatly affected by biologically synthesized silver nanoparticles and statistical analysis revealed that there was significant difference in cell permeability of two tested bacteria between control and treated cells with nanoparticles. However, there were slightly effect of these nanoparticles on cell integrity when the absorbance of DNA was estimated.

The Effect of Changing the Concentration of Silver Nanoparticles on the Antibacterial Properties of Silver-PVP Composite-Coated Implants

Background: The body’s ability to fight the infection in the vicinity of implants decreases, resulting in an increased risk of local infection around the implants. According to importance of this issue, this study investigates the antibacterial properties of implants coated via silver-PVP nanocomposite.
 Methods: In this research, silver nanoparticles have been synthesized by green synthesis method and using the coffee powder. The X-ray Diffraction (XRD) pattern confirms the formation of metallic structure of silver nanoparticles. Particle size has been studied by Transmission Electron Microscopy (TEM) and Digital Light Scattering (DLS) analysis, showing that the average particle size is about 17 nm. The implants have been coated via silver-PVP nanocomposite using deep coating method at different concentrations of Ag nanoparticles, and antibacterial properties of these samples have been investigated. The obtained results demonstrate a very large non-growth halo in the presence of silver-PVP coating. Also, the repeatability of non-growth halo test, toxicology analysis and pull off adhesion of samples have been studied and analyzed.
 Results: According to the MTT results, the appropriate concentration of silver-PVP nanocomposite without cell toxicity has been obtained equal to 118.6 µg/ml. At this concentration, the non-growth halo for both types of bacteria is clearly visible. By repeating the antibacterial test after 5 days, it was observed that the non-growth halo of implants is lasting.
 Conclusion: Overall, it can be stated that this study presents a simple, operational, repeatable, and large-scale method for coating the implants and medical equipment that prevents the development of infection in the vicinity of implants.

Multifunctional Cellulosic Natural Rubber and Silver Nanoparticle Films with Superior Chemical Resistance and Antibacterial Properties.

Composite films of natural rubber/cellulose fiber/silver nanoparticle were synthesized in a green route via the latex solution process. Hybrid cellulose filler containing carboxymethyl cellulose and cellulose microfibers was used to facilitate facile and fast preparation and to improve mechanical strength to the composites, respectively. All the composites possessed a high tensile strength of ~120 MPa, a high heat resistance of nearly 300 °C, and more than 20% biodegradability in soil in two weeks. Chemical resistance and antibacterial activity of the composite was enhanced depending on sizes and concentrations of silver nanoparticles (AgNPs). The composites containing 0.033-0.1% w/w AgNPs retarded toluene uptake to less than 12% throughout 8 h, whereas the composite containing 0.067-0.1% w/w AgNPs exhibited excellent antibacterial activities against Escherichia coli and Staphylococcus aureus. In comparison, 50 nm-AgNPs presented higher antibacterial activities than 100 nm-AgNPs. In vitro cytotoxicity test assessed after incubation for 24 h and 48 h revealed that almost all AgNPs-composite films exhibited non/weak and moderate cytotoxicity, respectively, to HaCaT keratinocyte cells.

Effect of silver nanoparticles incorporation on impact strength of Heat-cure denture base resins

Background:Poly (Methyl methacrylic acid) based materials are widely used for the fabrication of removable complete and partial denture prostheses. these materials are prone to fractures when subjected to impact forces. While recent studies have explored the antimicrobial potential of denture base materials incorporating silver nanoparticles, their impact on the mechanical properties crucial for the prosthesis's durability has not been adequately established.Aim:To evaluate the impact strength of the heat-cure denture base materials incorporated with various concentrations of silver nanoparticles. Materials and Methods:Silver nanoparticles were incorporated at various concentrations (0.5, 1.0, 2.0 and 5.0 wt%) into three different heat-cure denture base materials. A total of 150 rectangular-shaped specimens (50 x 6 x 4), which comprises 50 samples from each of the three heat-cure acrylic resins were made using the compression molding technique. Ten specimens (n=10) were allocated for each concentration such as control, 0.5wt%, 1.0wt%, 2.0wt% and 5.0wt% concentrations of silver nanoparticles. A “V” notch was made exactly at the centre of each specimen to prevent the ductile fracture. The impact strength was evaluated using the Izod impact tester. The data were subjected to One-way ANOVA and Post hoctests for statistical analyses.Results: A decrease in the impact strength was observed in the modified groups compared to the control groups. One-way ANOVA showed significant differences (p=0.000) among the different concentrations of all three denture base materials.Conclusion:Silver nanoparticles incorporation reduced the impact strength of the denture base materials.