Fabrication and characterization of silver-substituted bioactiveglass incorporated with sodium alginate and graphene oxide

Abstract

The temporary scaffold is fabricated as an adherent material for the implanted cells and physical support to direct the construction of the substitute tissues, with have high porous surface, large surface/volume ratio, high mechanical features, biocompatible, and biodegradable properties. This study aims to prepare a scaffold of bioactive glass(BG)/sodium alginate(SA)/graphene oxide(GO) composite doped with different concentrations of silver (Ag) nanoparticles with a sol-gel method. The obtained composites were evaluated by a variety of techniques (TGA, DSC, FTIR, XRD, SEM, and EDXA). Through an invitro test using simulated body fluid (SBF), the bioactivity of each specimen was investigated. Gram-positive bacteria were investigated for antimicrobial activity utilizing the agar diffusion method, such as (Staphylococcus aureus and Bacillus subtilis), gram-negative bacteria including (Pseudomonas aeruginosa and Escherichia coli), and fungi (Candida albicans). The bioactivity findings revealed that the surface of the prepared scaffold had formed a layer of hydroxyapatite (HA) as the amount of Ag was raised to (2%). This layer grew but decreased in (3%) and (4%) of Ag concentration, which assures the bioactivity of the prepared samples. The anti-microbial results exhibited a clearing inhibition zone around the samples which confirms the influence of silver doped composites on the proliferation of bacteria and fungi.