The Synthesis AgNPs of Leaves Melastoma malabathricum Extract reduce Adult Haemonchus contortus Motility Assay

Abstract

The research was performed to observe the effectiveness of silver nanoparticles (AgNPs) incorporate using the leaves Melastoma malabathricumin the ethanolic extract as anti-Haemonchus contortus. To determine the phytochemical content of Melastoma malabathricum, a qualitative phytochemical test was done. An adulticidal or an adult motility test was performed on mature female Haemonchus contortus. Three silver nanoparticle concentrations (AgNPs-Extract 0.2mgml-1, AgNPs-Extract 0.1mgml-1, AgNPs-Extract 0.05mgml-1) and two different ethanol extract Melastoma malabathricum concentrations (200mgml-1 and 100mgml-1) were examined. Albendazole 40mg/ml was used asa standard reference, and for negative control normal using saline water. The phytochemical analysis revealed that the ethanol extract of Melastoma malabathricum contains tannin, flavonoid, terpenoid, and steroid. The silver nanoparticles are identified visually as colour changes in the solution and using an ultraviolet-visible nanophotometer (UV-Vis). The spectrum was examined using a UV-Vis nanophotometer, and it revealed a clear peak around 365nm with a pH of 5.9. In a nematocidal assay on adult Haemonchus contortus, the paralysis time of the worm in silver nanoparticle (AgNPs- Extract 0.2mg/ml) was 8.85 minutes, which was significantly (P0.05) shorter than the control positive (14.07 minutes). Worms died in less than an hour in all treatments, and the negative control worm remained active for up to 2 hours after exposure