Signal Transfer Research Articles

Циліопатії, роль Wnt-сигнального шляху в перебігу гострого (постінфекційного) гломерулонефриту: огляд літератури та власне спостереження

Первинні війки — це субклітинні органели, що є похідними цитоплазматичної мембрани клітин майже будь-якого типу. Вони відіграють роль у сприйнятті та передачі механічних та хімічних сигналів із поверхні клітин, орієнтації площини поділу клітини, органогенезі тощо. Зазначені функції реалізуються завдяки великій кількості білків, рецепторів, сигнальних шляхів. Мутації та дерегуляція даних компонентів можуть мати широкий спектр фенотипових наслідків, що об’єднуються спільною назвою — циліопатії, до яких, зокрема, належать і кістозні ураження паренхіми нирок. Серед інших сигнальних каскадів, пов’язаних з первинними війками, Wnt-сигнальний шлях є відповідальним за нефрогенез та відповідь нирок на ушкоджуючі фактори. Описано клінічний випадок гострого (постінфекційного) гломерулонефриту в пацієнта Х., 20 років, із кістозним розширенням ниркових канальців, що було діагностовано гістологічно. У поданій статті, базуючись на останніх дослідженнях у галузі циліопатій, ми припускаємо ймовірні молекулярні механізми, відповідальні як за формування кіст у канальцевій системі нирок, так і за прогресуючий фатальний перебіг гломерулонефриту в даному випадку.

Extracellular Vesicles and Thrombosis: Update on the Clinical and Experimental Evidence.

Extracellular vesicles (EVs) compose a heterogenous group of membrane-derived particles, including exosomes, microvesicles and apoptotic bodies, which are released into the extracellular environment in response to proinflammatory or proapoptotic stimuli. From earlier studies suggesting that EV shedding constitutes a cellular clearance mechanism, it has become evident that EV formation, secretion and uptake represent important mechanisms of intercellular communication and exchange of a wide variety of molecules, with relevance in both physiological and pathological situations. The putative role of EVs in hemostasis and thrombosis is supported by clinical and experimental studies unraveling how these cell-derived structures affect clot formation (and resolution). From those studies, it has become clear that the prothrombotic effects of EVs are not restricted to the exposure of tissue factor (TF) and phosphatidylserines (PS), but also involve multiplication of procoagulant surfaces, cross-linking of different cellular players at the site of injury and transfer of activation signals to other cell types. Here, we summarize the existing and novel clinical and experimental evidence on the role and function of EVs during arterial and venous thrombus formation and how they may be used as biomarkers as well as therapeutic vectors.

Ecology and behavior predict an evolutionary trade‐off between song complexity and elaborate plumages in antwrens (Aves, Thamnophilidae)

The environment can impose constraints on signal transmission properties such that signals should evolve in predictable directions (Sensory Drive Hypothesis). However, behavioral and ecological factors can limit investment in more than one sensory modality leading to a trade-off in use of different signals (Transfer Hypothesis). In birds, there is mixed evidence for both sensory drive and transfer hypothesis. Few studies have tested sensory drive while also evaluating the transfer hypothesis, limiting understanding of the relative roles of these processes in signal evolution. Here, we assessed both hypotheses using acoustic and visual signals in male and female antwrens (Thamnophilidae), a species-rich group that inhabits diverse environments and exhibits behaviors, such as mixed-species flocking, that could limit investment in different signal modalities. We uncovered significant effects of habitat (sensory drive) and mixed-species flocking behavior on both sensory modalities, and we revealed evolutionary trade-offs between song and plumage complexity, consistent with the transfer hypothesis. We also showed sex- and trait-specific responses in visual signals that suggest both natural and social selection play an important role in the evolution of sexual dimorphism. Altogether, these results support the idea that environmental (sensory drive) and behavioral pressures (social selection) shape signal evolution in antwrens.

АВТОМАТИЗИРОВАННАЯ ТРАНСПОРТНО-ТЕХНОЛОГИЧЕСКАЯ СИСТЕМА ОРГАНИЗАЦИИ ДОРОЖНОГО ДВИЖЕНИЯ И СОЗДАНИЯ ТРАНСПОРТНЫХ КОРИДОРОВ

There is considered a problem of road traffic organization at crossroads under conditions of urban environment and outgoing highways. The urgency of the problem of automated traffic control with the information for vehicle drivers about traffic situation using both navigation systems and warning systems by means of signal transfer to radar-detectors is shown. 
 The investigation purpose consists in the development of automated self-regulated system of traffic lights control on the basis of vehicle number approaching to the traffic lights and vehicle driver warning about traffic jam approaching for the purpose of its going around. 
 In the paper there are solved problems regarding traffic capacity of a megapolis traffic system, driver warning about problem road situations and the automation of these processes. On the basis of the theory of automated control there is shown a developed system allowing the formation of a traffic passage by means of driver warning about traffic jam ahead. There are described thoroughly component parts of the system limiting only with the choice of components among prototypes available in the market. 
 A principle of automated traffic flow control at the crossroads supplied with the traffic lights is shown. There is shown a functional diagram of the interaction of system components for the successful traffic organization. The algorithm of the system automation functioning with its adjustment to certain time intervals of the effect of the enabling traffic light signal at a particular T-shaped or X-shaped intersection is described. The system developed for traffic control at intersections allows relieving considerably city streets. The analysis of the system has shown that traffic lights adaptive control allows decreasing traffic up to 70%. The application of domestic software components decreases system cost by 2-4 times as compared with foreign prototypes which, among other things, do not have the functional offered.

Mechanical and Electronic Cell-Chip Interaction of APTES-Functionalized Neuroelectronic Interfaces.

In this work, we analyze the impact of a chip coating with a self-assembled monolayer (SAM) of (3-aminopropyl)triethoxysilane (APTES) on the electronic and mechanical properties of neuroelectronic interfaces. We show that the large signal transfer, which has been observed for these interfaces, is most likely a consequence of the strong mechanical coupling between cells and substrate. On the one hand, we demonstrate that the impedance of the interface between Pt electrodes and an electrolyte is slightly reduced by the APTES SAM. However, this reduction of approximately 13% is definitely not sufficient to explain the large signal transfer of APTES coated electrodes demonstrated previously. On the other hand, the APTES coating leads to a stronger mechanical clamping of the cells, which is visible in microscopic images of the cell development of APTES-coated substrates. This stronger mechanical interaction is most likely caused by the positively charged amino functional group of the APTES SAM. It seems to lead to a smaller cleft between substrate and cells and, thus, to reduced losses of the cell's action potential signal at the electrode. The disadvantage of this tight binding of the cells to the rigid, planar substrate seems to be the short lifetime of the cells. In our case the density of living cells starts to decrease together with the visual deformation of the cells typically at DIV 9. Solutions for this problem might be the use of soft substrates and/or the replacement of the short APTES molecules with larger molecules or molecular multilayers.

Network and Field Analysis of Koch Snowflake Fractal Geometry Radiofrequency Coils for Sodium MRI

Sodium is one of the most abundant physiological cations and is a key element in many cellular processes. It has been shown that several pathologies, including degenerative brain disorders, cancers, and brain traumas, express sodium deviations from normal. Therefore, sodium magnetic resonance imaging (MRI) can prove to be valuable for physicians. However, sodium MRI has its limitations, the most significant being a signal-to-noise ratio (SNR) thousands of times lower than a typical proton MRI. Radiofrequency coils are the components of the MRI system directly responsible for signal generation and acquisition. This paper explores the intrinsic properties of a Koch snowflake fractal radiofrequency surface coil compared to that of a standard circular surface coil to investigate a fractal geometry’s role in increasing SNR of sodium MRI scans. By first analyzing the network parameters of the two coils, it was found that the fractal coil had a better impedance match than the circular coil when loaded by various anatomical regions. Although this maximizes signal transfer between the coil and the system, this is at the expense of a lower Q, indicating greater signal loss between the tissue and coil. A second version of each coil was constructed to test the mutual inductance between the coils of the same geometry to see how they would behave as a phased array. It was found that the fractal coils were less sensitive to each other than the two circular coils, which would be beneficial when constructing and using phased array systems. The performance of each coil was then assessed for B1+ field homogeneity and signal. A sodium phantom was imaged using a B1+ mapping sequence, and a 3D radial acquisition was performed to determine SNR and image quality. The results indicated that the circular coil had a more homogeneous field and higher SNR. Overall while the circular coil proved to generate a higher signal-to-noise ratio than the fractal, the Koch coil showed higher versatility when in a multichannel network which could prove to be a benefit when designing, constructing, and using a phased array coil.

The Relationship Between Body Composition And Y-balance Testing In D2 Collegiate Athletes

PURPOSE: The purpose of this study was to identify if and how body composition plays a role on dynamic balance in collegiate athletes. METHODS: 154 Division II collegiate athletes (mean ± SD; age = 19.81 ± 1.46 years; height = 171.8 ± 11.12 cm; weight = 71.64 ± 14.05 kg) completed paperwork including informed consent, a health history questionnaire, and PARQ. Participants completed a dynamic warm-up on a stationary bicycle followed by the Y-Balance testing. Participants completed a maximal reach (cm) in three directions; anterior (AR), posterior lateral (PLR), and posterior medial (PMR) for right (RL) and left (LL) legs. Participants maintained single leg balance while reaching far as possible with the other leg for three trials in each direction. The furthest reach for each direction was used for data analysis. Body composition was assessed using Bioelectrical Impedance Analysis (BIA). Participants gripped the BIA handles allowing transfer of electrical signal from the BIA to the participant. Measures included body fat percentage (BFP), body fat mass (BFM), and fat free mass (FFM). Using SPSS, Pearson R-correlations were used to analyze relationships between balance measures and body composition measures with a p-value set at p < 0.05. RESULTS: There was a negative, weak, significant correlation between RLAR and BFM. (p = .041, r = -.171). There were a negative, weak, significant correlation between RLPMR and BFP (p = .006, r = -.227) but a positive, weak, significant correlation between RLPR and fat free mass (p = .001, r = .267). There was a negative, weak, significant correlation between LLAR and BFM (p = .007, r = -.224) and a negative, weak, significant correlation between LLAR and BFP (p = .002, r = -.258). There was a negative, weak, significant correlation between LLPLR and BFP (p = .030, r = -.181) but a positive, small, significant correlation between LLPLR and FFM. (p = .024, r = .189). Lastly, there was a negative, weak, significant correlation between LLPMR and BFP (p = .010, r = -.213) but a positive, weak, significant correlation between LLPMR and FFM (p = .000, r = .288). There were no significant correlations between all other correlations. CONCLUSIONS: Overall higher body fat has detrimental influence on balance; however, further research should investigate the extent to which fat free mass could benefit balance.

Bull Sperm Capacitation Is Accompanied by Redox Modifications of Proteins.

The ability to fertilise an egg is acquired by the mammalian sperm during the complex biochemical process called capacitation. Capacitation is accompanied by the production of reactive oxygen species (ROS), but the mechanism of redox regulation during capacitation has not been elucidated. This study aimed to verify whether capacitation coincides with reversible oxidative post-translational modifications of proteins (oxPTMs). Flow cytometry, fluorescence microscopy and Western blot analyses were used to verify the sperm capacitation process. A fluorescent gel-based redox proteomic approach allowed us to observe changes in the level of reversible oxPTMs manifested by the reduction or oxidation of susceptible cysteines in sperm proteins. Sperm capacitation was accompanied with redox modifications of 48 protein spots corresponding to 22 proteins involved in the production of ROS (SOD, DLD), playing a role in downstream redox signal transfer (GAPDHS and GST) related to the cAMP/PKA pathway (ROPN1L, SPA17), acrosome exocytosis (ACRB, sperm acrosome associated protein 9, IZUMO4), actin polymerisation (CAPZB) and hyperactivation (TUBB4B, TUB1A). The results demonstrated that sperm capacitation is accompanied by altered levels of oxPTMs of a group of redox responsive proteins, filling gaps in our knowledge concerning sperm capacitation.

Enzyme Mimic Facilitated Artificial Cell to Mammalian Cell Signal Transfer

AbstractCatalyzing biochemical reactions with enzymes and communicating with neighboring cells via chemical signaling are two fundamental cellular features that play a critical role in maintaining the homeostasis of organisms. Herein, we present an artificial enzyme (AE) facilitated signal transfer between artificial cells (ACs) and mammalian HepG2 cells. We synthesize metalloporphyrins (MPs) based AEs that mimic cytochrome P450 enzymes (CYPs) to catalyze a dealkylation and a hydroxylation reaction, exemplified by the conversion of resorufin ethyl ether (REE) to resorufin and coumarin (COU) to 7‐hydroxycoumarin (7‐HC), respectively. The AEs are immobilized in hydrogels to produce ACs that generate the two diffusive fluorophores, which can diffuse into HepG2 cells and result in dual intracellular emissions. This work highlights the use of AEs to promote AC to mammalian signal transfer, which opens up new opportunities for integrating the synthetic and living world with a bottom‐up strategy.

Enzyme Mimic Facilitated Artificial Cell to Mammalian Cell Signal Transfer.

Catalyzing biochemical reactions with enzymes and communicating with neighboring cells via chemical signaling are two fundamental cellular features that play a critical role in maintaining the homeostasis of organisms. Herein, we present an artificial enzyme (AE) facilitated signal transfer between artificial cells (ACs) and mammalian HepG2 cells. We synthesize metalloporphyrins (MPs) based AEs that mimic cytochrome P450 enzymes (CYPs) to catalyze a dealkylation and a hydroxylation reaction, exemplified by the conversion of resorufin ethyl ether (REE) to resorufin and coumarin (COU) to 7-hydroxycoumarin (7-HC), respectively. The AEs are immobilized in hydrogels to produce ACs that generate the two diffusive fluorophores, which can diffuse into HepG2 cells and result in dual intracellular emissions. This work highlights the use of AEs to promote AC to mammalian signal transfer, which opens up new opportunities for integrating the synthetic and living world with a bottom-up strategy.

Wealth Effects of Dividend Announcements on Bondholders: The Case of Taiwan Bond Market

Although bonds play an important role in U.S. capital markets, this financial instrument is less common in the emerging markets. Taiwan is a typical case. In fact, both bond issuances and bond transactions in Taiwan have been declining in the past years. Consistent with the previous studies, this research documents that wealth transfer effects exist between bondholders and stockholders. We hypothesize that this wealth transfer discourages investors from investing in bond markets because companies in Taiwan seem to care less about the interest of bondholders. Using the event study methodology, we examine the price change of bonds and stocks in Taiwan capital market around cash dividend announcements. We find that there are significant abnormal returns before cash dividend announcements from 30 days to 60 days and that there is insignificant price change of bonds during the three-day period around the announcement. Possible explanations of the results include low bond trading volumes, insider trading before announcements, and mixing signaling and wealth transfer effects. Although this study cannot prove that the results are directly related to management holdings, we tend to believe that insider trading somehow matters.

Precise Time Synchronization and Clock Comparison Through a White Rabbit Network‐Based Optical Fiber Link

AbstractPrecise transfer of time and frequency signals over long distances as well as clock synchronization to an ultra‐stable reference are very crucial for many of the technological applications as well as for advanced scientific research. These reinforces a wide range of applications such as navigation, power grid management, mobile communication, and so on. In order to compare the performances of two highly stable and accurate atomic clocks, it is desirable that the link between the two clocks, that is, the transmission link has higher level of stability than those clocks. This article describes establishment of an ultra‐stable optical fiber link employing White Rabbit network for transfer of time and frequency signals and also for comparing performance of atomic clocks. Utilizing this link, time signals have been transferred within an uncertainty of ±130 ps at ambient temperature (30°C–40°C) and the instability of the link in terms of modified Allan‐deviation reaches to ∼10−16 within one day of integration time.

Maternal exposure to sodium ρ-perfluorous nonenoxybenzene sulfonate during pregnancy and lactation disrupts intestinal barrier and may cause obstacles to the nutrient transport and metabolism in F0 and F1 generations of mice

Sodium ρ-perfluorous nonenoxybenzene sulfonate (OBS), a novel kind of perfluoroalkyl and polyfluoroalkyl compound, has been widely detected in the environment. The toxicity of OBS to living organisms has become a public concern. A growing body of research showed that maternal exposure to environmental pollutants caused intestinal and metabolic diseases that could be conserved across offspring. Here, female C57BL/6 mice were treated OBS at dietary levels of 0.0 mg/L (CON), 0.5 mg/L (OBS-L) and 5.0 mg/L (OBS-H) during the gestation and lactation periods. The results demonstrated that OBS treatment not only induced significant changes in the mucus secretion and ionic transport, but also disrupted the expression of antimicrobial peptides (AMPs) in the intestine of F0 and F1 generations. Additionally, OBS exposure altered bile acids metabolism and affected the transcriptional levels of critical genes involved in bile acids synthesis, signaling transfer, transportation and apical uptake. Together, all these results indicated that OBS exposure was perceived as a major stress by the intestinal epithelium that strongly affected the intestinal barrier function (including mucus, CFTR, AMPs, inflammation), and ultimately led to imbalance in the metabolism of bile acids (BAs). Moreover, we found that maternal OBS exposure had a more obvious toxicity effect on the male offspring in this experiment. Taken together, maternal OBS exposure during pregnancy and lactation had the intestinal and metabolism toxic effects on the dams and offspring, indicating that effects of maternal exposure on the toxicity of offspring could not be ignored.

Abstract 2740: Effect of pancreatic cancer cell derived extracellularvesicles on the tumor microenvironment

Abstract Introduction: Pancreatic ductal adenocarcinoma (PDAC) is one amongst the most lethal malignancies, with a 5 year survival rate of &amp;lt;10%.While the tumor microenvironment in PDAC is highly immunosuppressive, little is known about the mechanisms of immune invasion leading to rapid tumor progression. Hence, in-depth exploration of early cellular and molecular drivers of PDAC is desirable for early and precise diagnosis and to identify new biological targets for developing new therapies. Extracellular vesicles (EVs) are nanometer sized particles that are shed by most tissues/organs and are known to be important mediators of intercellular signaling and molecular transfer. We hypothesized that pancreatic cancer derived EVs may play an important role in mediating the interactions among the tumor and surrounding microenvironment (TME). Given that tumor-associated macrophages (TAMs) are integral components of the TME that can promote tumor progression, tumor invasion, and intravasation to other parts of the body, this study is focused on investigating the role of PDAC derived EVs in macrophages polarization, which in turn would impact tumor cell proliferation and invasion. Results: EVs were isolated from 6 pancreatic cancer (PaCa) and 2 normal established pancreatic epithelial cell lines. EVs were characterized using nanoparticle tracking (NTA) and immunoblot analysis. LC-MS/MS based shotgun proteomics analyses of EVs revealed that PaCa cell line derived EVs were significantly enriched in potentially immunomodulatory proteins such as S100 calcium-binding protein A4 (S100A4) and human leukocyte antigen-B (HLA-B). However, PaCa cell EVs had significantly less MHC-class-I-related chain A (MICA), a protein important for proper human leukocyte antigen (HLA) function, as compared to normal pancreatic epithelial cell derived EVs. These data suggest that PaCa derived EVs may influence immunomodulation and macrophage function within the tumor microenvironment thereby strengthening our hypothesis. Further experimental studies aimed at delineating the precise mechanism of action of EVs in macrophage polarization is ongoing. Conclusions: Our finding suggests significant difference among the molecular composition of PaCa derived cargos as compared to and normal cells derived EVs that could be useful in furthering our understanding about pancreatic cancer etiology. Furthermore, the novel insights obtained from these studies may augment the development of biomarkers that can be used for early detection of the disease and improved therapeutics to improve clinical outcomes. Citation Format: Baldev Singh, Charles Hinzman, Edina Wang, Anton Iliuk, Jose Trevino, Keith Unger, Partha Banerjee, Amrita K. Cheema. Effect of pancreatic cancer cell derived extracellularvesicles on the tumor microenvironment [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 2740.

Fiber Radio Frequency Transfer Using Bidirectional Frequency Division Multiplexing Dissemination

We report on the realization of a novel fiber-optic radio frequency (RF) transfer scheme with the bidirectional frequency division multiplexing (FDM) dissemination technique. Here, the proper bidirectional frequency map used in the forward and backward directions for suppressing the backscattering noise and ensuring the symmetry of the bidirectional transfer RF signals within one telecommunication channel. We experimentally demonstrated a 0.9 GHz signal transfer over a 120 km optical link with the relative frequency stabilities of 2.2E-14 at 1 s and 4.6E-17 at 20,000 s. The implementation of phase noise compensation at the remote site has the capability to perform RF transfer over a branching fiber network with the proposed technique as needed by large-scale scientific experiments.

E-Commerce at the Bottom of the Pyramid: Remodelling Perspectives of Rural Consumer Behaviour by Pragya Singh, Indrajit Ghosal and Bikram Prasad. New Delhi: Anu Books, 2020 (ISBN: 978-81-947224-1-0)

Marketing infrastructure includes all those facilities and amenities needed for the smooth conduct of marketing in the economy. The infrastructural facilities in development are as necessary as foundations of a building. The existence of adequate marketing infrastructure are important not only for the performance of various marketing functions and expansion of the size of the markets but also for the transfer of appropriate price signals leading to improved marketing efficiency. The availability of different infrastructures affects the choice of technology to be adopted, reduces the cost of transportation, produces powerful impetus to production and also affects income distribution in favour of small and marginal farmers by raising their access to the market. The agriculture sector needs heavy investment for creation of basic infrastructures necessary for the overall economic development. In a developing country like India, marketing infrastructures play a pivotal role in fostering and sustaining the tempo of rural and economic development. Marketing is as critical to better performance in agriculture as farming itself. Though the role of infrastructure is the key element of any development programme yet their role in distribution and marketing is the supreme. India's growth both as agriculturally and horticultural advanced country may get derailed if various marketing infrastructural constraints are not removed. Many of the regions of the country still suffer from the existence of infrastructural problems that they threaten to torpedo the region's agricultural and horticultural development efforts. In this paper an attempt has been made to examine the status of different agricultural marketing infrastructures, their geographical spread in the different states of India and also the policy measures for strengthening of these infrastructural facilities. Besides this other marketing infrastructure, which has been set up in the specialized form of organization, has also been just mentioned for information.

Smart Automation System for Controlling Appliances using Mobile Device

Electronic devices and appliances have become very common in this recent year of technology especially with fast development in smartphones. In this paper, the design of Home automation system with good feature of automation via mobile device are presented. Bluetooth Based smart Automation System Using Android and Arduino is design and implemented. Bluetooth Based Automation System Using Android application and Arduino is implemented and design. This paper describe about smart automation system which would use to enable home lighting, fan, cooler, water pumping motor using a smart phone application with Bluetooth wireless technology. The system included three main components an Arduino Uno for connecting the appliances, a Bluetooth module for signal transfer, and a smartphone with the Android application to control home appliances. The idea of paper is to control home appliances to avoid the dangerous of electric shock and convenience of decrepit physically disable people, who can easily access and control the home appliances by staying at particular place and access them through smart phone without the help of other people.

Lack of support for surface diffusion of postsynaptic AMPARs in tuning synaptic transmission

Repetitive stimulation of excitatory synapses triggers molecular events required for signal transfer across neuronal synapses. It has been hypothesized that one of these molecular events, the diffusion of extrasynaptic α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPARs) (i.e., the diffusion hypothesis), is necessary to help synapses recover from paired-pulse depression. To examine this presumed role of AMPAR diffusion during repetitive presynaptic stimulation, a biophysical model based on published physiological results was developed to track the localization and gating of each AMPAR. The model demonstrates that AMPAR gating in short intervals of fewer than 100 ms is controlled by their position in relation to the glutamate release site and by their recovery from desensitization, but it is negligibly influenced by their diffusion. Therefore, these simulations failed to demonstrate a role for AMPAR diffusion in helping synapses recover from paired-pulse depression.

Drosophila females receive male substrate-borne signals through specific leg neurons during courtship

SummarySubstrate-borne vibratory signals are thought to be one of the most ancient and taxonomically widespread communication signals among animal species, including Drosophila flies.1, 2, 3, 4, 5, 6, 7, 8, 9 During courtship, the male Drosophila abdomen tremulates (as defined in Busnel et al.10) to generate vibrations in the courting substrate.8,9 These vibrations coincide with nearby females becoming immobile, a behavior that facilitates mounting and copulation.8,11, 12, 13 It was unknown how the Drosophila female detects these substrate-borne vibratory signals. Here, we confirm that the immobility response of the female to the tremulations is not dependent on any air-borne cue. We show that substrate-borne communication is used by wild Drosophila and that the vibrations propagate through those natural substrates (e.g., fruits) where flies feed and court. We examine transmission of the signals through a variety of substrates and describe how each of these substrates modifies the vibratory signal during propagation and affects the female response. Moreover, we identify the main sensory structures and neurons that receive the vibrations in the female legs, as well as the mechanically gated ion channels Nanchung and Piezo (but not Trpγ) that mediate sensitivity to the vibrations. Together, our results show that Drosophila flies, like many other arthropods, use substrate-borne communication as a natural means of communication, strengthening the idea that this mode of signal transfer is heavily used and reliable in the wild.3,4,7 Our findings also reveal the cellular and molecular mechanisms underlying the vibration-sensing modality necessary for this communication.

Acoustic emission source location from P-wave arrival time corrected data and virtual field optimization method

Acoustic emission (AE) event location plays an important role in structural safety assessments. However, accurately locating an AE event is usually difficult, especially for a small structure. Interestingly, a pencil-lead break (PLB) experiment shows that the P-wave arrival time of a sensor is always significantly different (~260 µs) from that of the other sensors. This time difference is much greater than the P-wave travel time in the range of the experimental sample. Therefore, it can be inferred that there is a P-wave arrival time system error (PATSE) for each sensor. The PATSE may be due to a combined result of the sensor site effect and signal transfer delay time from the sensor to the signal storage. To handle this, a Bayesian inversion framework was built to estimate PATSEs. A synthetic test demonstrated the effectiveness of the proposed Bayesian method for noisy P-wave arrival time data. Then, Bayesian inversion was applied to 15 PLB events, which confirmed the existence of PATSE in an AE experiment for the first time. The average PATSE reached 1.47 µs without considering the P-wave arrival time significantly different sensor. The average location error of 25 PLB events was 14.30 mm and 6.58 mm for PATSE unremoved and removed data, respectively. To achieve this, a high-precision virtual field optimization location method (VFOM) was used. This demonstrates the necessity of removing the PATSEs. Finally, the AE event location performance for the PATSE unremoved and removed data was compared, where the AE events were obtained from the uniaxial compression of a red sandstone sample. The results indicated that there was a higher location detection success rate for the corrected data. The AE locations based on the corrected data were in a better correlation with the rock sample failure mode than that without correction. Moreover, increase the signal sampling frequency for AE event identification, use a real-time inverted 3D velocity model and update the PATSEs in real time could be used to further improve the AE event location accuracy.