Siddha Literature Research Articles

Screening of anti-diabetic potential of the siddha formulation sambirani poo kuligai by in-vitro alpha glucosidase enzyme inhibition assay

Background: Noninsulin-dependent diabetes mellitus (NIDDM) is a common disease of the endocrine system caused by the decreased secretion of insulin by the pancreatic Langerhans beta cell or by the lowering of insulin resistance due to excessive absorption of glucose. The Siddha system of medicine is a traditional medical system that uses a scientific and holistic approach to provide preventive, promotive, curative, rejuvenative and rehabilitative health care. According to Siddha literature, diabetes is known as Innippu Neer, Madhumegam and Neerizhivu. The various reasons for the cause of this disease are attributed to foodhabits and lifestyle changes and also due to hereditary causes. One of the therapeutic approaches is to decrease the postprandial hyperglycemia by retarding the absorption of glucose through the inhibition of carbohydrate-hydrolyzing enzymes, such as a-amylase and ?-glucosidase. ?-Glucosidase are enzyme that catalyze the absorption of digested glucose from dietary polysaccharides in the small intestine. Aim: The aim of the present study is to evaluate the anti-diabetic potential of the siddha formulation of Sambirani Poo Kuligai(SPK) by alpha glucosidase enzyme inhibition assay. Method: The spectrophotometric assay method was used to find alpha glucosidase inhibitory activity. Results: SPK showed presence of alpha glucosidase inhibitory potential with the maximum inhibition of about 41.74 ± 3.578 % and the corresponding IC50 is 606.5 ± 49.51?g/ml. Conclusion: This in-vitro study revealed the presence of the alpha glucosidase activity in the siddha formulation Sambirani Poo Kuligai possesses antidiabetic activity

Physicochemical and phytochemical analysis of siddha formulation thoothuvalaiyathi chooranam

Aim And Objective: This study aimed to investigate the physicochemical properties and phytochemical composition of siddha formulation thoothuvalaiyathi chooranam. Materials And Methods: Thoothuvalaiyathi Chooranam is one Of the Siddha formulation chosen from Sarabenthira vaithya muraigal kasa swasa sikichai , Page No. 153 which is indicated for Eraippu ,Kasam ,swasam, kabarogangkal. The drug prepared as per the Siddha literature. The drug is subjected to physico chemical analysis such as loss on ignition, loss on drying, water soluble ash etc and phytochemical analysis as per the PLIM guidelines. Results And Conclusion: Phytochemical analysis of this drug was done such as pH (7.10), Loss on ignition (92.10%), Acid Insoluble ash (5.10±0.100), Water soluble extractive (9.30±0.500), Alcohol soluble extractive (8.10±0.200),Loss on drying (0.90±0.500) .The results revealed the presence of various bioactive compounds, including Alkaloids, Carbohydrates, Glycosides, Saponins, Phenols, Triterpenoids, Flavanoids, Proteins and amino acids, Anthocyanin , Tannins.The results indicate the standard quality ,safety and standardization for their use.

SIDDHA HERBS AND MEDICINES FOR THE TREATMENT OF BREAST CANCER WITH EMPHASIS ON CELL LINE ACTIVITY: A REVIEW

Breast cancer is the most common cancer in women worldwide. It causes major morbidity and mortality. Current treatment options for breast carcinoma include surgery, chemotherapy, radiotherapy and hormone therapy.The main challenge lies in treating the disease with fewer side effects and patient compliance as the above-mentioned therapies cause general as well as local complications. Siddha literature has many herbal, metallic and mineral preparations to treat breast cancer which can be used as an adjunct for treating breast cancer. For this review, the literature on diagnosis and treatment for breast cancer was collected from multiple sources like Siddha classical texts, traditional books, Siddha cyclopaedic medical dictionary. The main objective of this paper is to evaluate, document and discusses the important Siddha herbal, herbo-mineral and external medicines with Botanical name, Tamil name, Pharmacological activity and cell line model used in treatment of breast carcinoma.

THERAPEUTIC EFFICACY OF ASHTABHAIRAVA MATHIRAI IN FEVER MANAGEMENT: A REVIEW

Ashtabhairava mathirai is an herbomineral formulation used in the Siddha system. Its application in treating fever has been described in the Siddha Vaidhiya Thirattu textbook, Hence, a review of the ingredients is essential. This review aims to critically assess the available literature on the indication of Ashtabhairava mathirai in treating fever, its therapeutic potential, and its mechanism of action. A comprehensive literature search was conducted across multiple databases, including Siddha literature. The ingredients of Ashtabhairava mathirai exhibit various pharmacological activities, such, as antipyretic, anti-inflammatory, antimicrobial, and antioxidant activity. The study concluded that the multiple actions of ingredients of Ashtabhairava mathirai which is used to treat the condition, fever. These findings support the traditional therapeutic value of Ashtabhairava mathirai in treating 64 types of fever.

Serological Evaluation of the Anti-coagulant Activity of the Classical Siddha Medicine -‘Veera Mezhugu’ in Wistar Albino Rats

In setting the basis for the management of arterial thrombosis, cardiac ischemic changes followed by any severe coronary atherosclerotic changes or any venous thrombosis, there is a conventional use of Acute thrombolytic agents, Anticoagulants with antiplatelet medications in general. These symptoms complex are mentioned as Thamaraga vaayu or Ruththira vaayu or Maaradaippu(Acute coronary syndrome) and Uthirakattu(thromboembolism) in siddha literatures. Veera mezhugu is one of the Siddha formulations chosen from Anuboga vaithiya navaneetham part-4, which is indicated for Uthirakattu and Thamaragavaayu. The drug prepared as per the Siddha literature. The drug has undergone safety studies and the efficacy studies in Wistar albino rats where the serum of the different groups with different doses are compared with the placebo control to evaluate the anti-coagulant potential. Coagulation profile of the three parameters of Activated partial thromboplastin time(APTT), Prothrombin time(PT) and International normalized ratio(INR) are taken for evaluation and had significant difference between the groups[p<0.05].Serological analysis of the Wistar rats shows significant anticoagulant activity in a dose dependent manner.

Identifying the molecular mechanisms of action of wound healing properties of Mathan tailam and Mahamegarajanga tailam in Wistar albino rats: Evidence through IL-10/VEGF/TNF-α signalling pathways

The Siddha system of medicine (SSM) is the oldest medical science practised in the ancient period of the southern part of India and Sri Lanka. Many formulations were described for wound healing in the SSM, with specific diagnostic differentiation in the Siddha literature. Most preparations for wound healing were available in the form of oil-based formulations, especially for external usage. Mathan tailam (MT) and Mahamegarajanga tailam (MMRT) have been used by Siddha physicians and traditional practitioners to treat wounds. Mathan tailam is a popular regimen for skin lacerations, burns, skin infections, diabetic wounds, and dermatitis. Mahamegarajanga tailam has long been used by traditional vaidyars to treat cuts and burns. Both MT and MMRT are clinically well-appreciated drugs for wound healing and need to be studied for their mechanisms of action for scientific documentation. In an in vivo study on albino rats -excisional wound model, the histopathological changes, histo-immune response, biomarker analysis, and mRNA expression were studied and analysed. Wounds treated with MT and MMRT healed faster (p < 0.05) than the untreated group (CNT). Histological investigation showed rapid re-epithelialization, dense collagen deposition, increased enzymatic antioxidant activities and decreased lipid peroxidation in the MT and MMRT groups. mRNA expression reveals MT and MMRT-treated tissues able to induce convergent cell motility in wound space. Our study for the first time provides strong in vivo experimental evidence that Mathan tailam and Mahamegarajanga tailam play a crucial role in promoting skin tissue wound healing through IL-6/VEGF/TNF-α mediated mechanisms. Traditional practices continue to teach us valuable lessons, as seen by their continuous use in their locality for years.

A Literature Review on Siddha Medicines for KAMALAI NOI (JAUNDICE) Mentioned in Siddha Literatures

Background: Yellow discolourisation of the some areas in our body such as skin, sclera, mucous membrane. The clinical features of Jaundice may be correlated with Kamalai noi.In Siddha literature Balavagadam, kamalai noi is divided in to three types namely Oodhu Kamalai,Manjal Kamalai, Varal Kamalai.  Objective: To document the collective data of medicines for KAMALAI NOI (JAUNDICE)mentioned in various Siddha literatures.  Materials and Methods: The data about the jaundice and its treatment are collected from Government Siddha Medical College library, SCRI – Library, Anna Centenary – Library, Thamarai Library, Connemara – Library etc. Siddha literature such as sikicha rathna deepam, Theraiyar vagadam, Agasthiyar vaithiya rathna surukkam, pillaipini maruthuvam, Balavagadam, Sarabendra vaithiya muraikal etc  Result: This Descriptive study document the medicines for KAMALAI NOI (JAUNDICE) mentioned in various Siddha literatures. This review can be useful for siddha physicians to choose better medicines for the treatment of jaundice and also useful for further research study which is needed for standardization and evaluvation of jaundice medications.  Conclusion: I conclude that this review will be beneficial for further research and also useful for treatment and prevention of Jaundice in siddha system of medicine

Assessment of Knowledge and Practices of Chathru Unavugal (Compatible Food) and Mithru Unavugal (Incompatible Food) According to Siddha Literature Among Patients Attending Out Patients Department in GSMC Hospital, Palayamkottai

The science of correctly combining food is greatly overlooked in the world of food and nutrition. But in this contemporary world several proponents of combined food eating procedures are followed till now. Mixing of wrong food leads to stomach upset, dyspepsia, bloating abdomen and other digestive ails. In Siddha literature “Noi illa Neri”enuciate that mixing of food having different potency and taste become poisonous. yet, it also describes every food substance has its own ill effect these are nullified by adding Chathru (antagonistic items). Correspondingly intake of incompatible food is on rise in present era. In this paper, we are supposed to identify some literature evidence based new food incompatibilities for 300 participants which are used on day-to-day life, based on Siddha perspective. This is nothing but the primordial prevention. Keywords: Combining food, Nutrition, Siddha, Noi illa neri.

Exploring the Molecular Docking Interactions between the Polyherbal Formulation Ibadhychooranam and Human Aldose Reductase Enzyme as a Novel Approach for Investigating its Potential Efficacy in Management of Cataract

Background: Kannir sirandha urupillai... which means no organ is better than eye. Eye health and treatment have held significant importance in the Siddha system of medicine since ancient times, emphasizing the holistic approach to well-being encompassing physical, mental, and spiritual aspects. Eye disease is classified into 96 types and their ethology, treatment method, preventive measures all are explained in many of the Siddha literature. Among them cataract is one of the leading cause of blindness which is characterised by clouding of eye’s natural lens, leading to blurry, foggy or flimy vision. This paper will elaborates about the docking analysis of poly herbal formulation Ibadhy chooranam against Human aldose reductase enzyme for cataract.  Aim &amp; Objective: The aim of this study is to investigate the potential efficacy of the polyherbal formulation IbadhyChooranam in preventing or treating cataracts through molecular docking analysis of its interactions with the human aldose reductase enzyme.  Methodology: Docking simulations were conducted for the extracted phytoconstituents of IbadhyChooranam against the Human Aldose Reductase Enzyme. AutoDock tools were utilized to incorporate hydrogen atoms, Coleman united atom type charges, and solvation parameters. The docking process employed the Lamarckian genetic algorithm along with the Solis &amp; Wets Local Search method to simulate ligand-receptor interactions.  Result: The current study revealed that phytochemicals present in IbadhyChooranam, including Nerolidol, Ellagic acid, Phyllanthin, Costunolide, Embelin, Cyperolone, Zingiberene, Piperic acid, Piperine, and Lupeol, exhibited between 6 to 9 significant interactions with residual amino acids in the aldose reductase enzyme. In comparison, the standard drug Epalrestat demonstrated 8 viable interactions with the residual amino acids of the aldose reductase enzyme.  Conclusion: According to the computational analysis findings, it can be inferred that the bioactive compounds present in IbadhyChooranam exhibit notable binding affinity towards the target aldose reductase enzyme. Ibadhy chooranam inhibit the function of aldose reductase enzyme which delay the sorbitol accumulation and subsequent cataract development. Through this study, it was also justified that the Ibadhy chooranam possess significant anti-cataract activity.

Clinical evaluation of therapeutic efficacy of a Siddha herbo mineral formulation “Kumaara Veeriya Kaantha Chenduram’’ with “Nellikkai Legiyam” (internal) in the treatment of Iron Deficiency Anemia

Abstract: Background: Kumaara veeriya kaantha chenduram (KVKC) is a siddha herbomineral formulation indicated for paandunoi in Siddha literature (1).Paandu noi is a disease characterized by changes in the body's natural color andpallor found in the skin, nails and conjunctiva. Objectives: To determine the efficacy of the KVKC, the open labed clinical study was conducted in National Institute of Siddha OPD. Materials and Methods: The 30 patients with Iron deficiency anaemiawere selected and treated with KVKC orally with the dose of 260 milli gram twice in a day with Nellikailegiyam6 gram continuously for 45 days. Laboratory investigations for hemoglobin was done on 1st day and every 15 days once of the study. Results: Clinical assessment was done, based on the before and after treatment scores which was compared statistically and recorded.Regarding Hemoglobin level, 24(80%) patients showed increase of 1 to 3 grams from base level, 6(20%) patients showed increase of 0.1 to 0.9 grams from base level.Regarding Serum Ferritin, out of 30 patients, 9 (30%) patients showed 0.1-3.5 ng/ml increase from its base level, 21 (70%) patients showed 3.6-7.0 ng/ml increase from its base level.From the Statistical studies, the Mean ± Standard deviation for Haemoglobin before treatment was 9.7 ± 0.93 and after treatment was 11.18 ± 1.08 which was statistically highly significant (p&lt;0.0001).And Serum ferritin before treatment was 9.27 ± 3.31, after treatment was 13.5 ± 3.49 which was statistically significant (p&lt;0.0001).

A LITERATURE REVIEW ON MEDICATIONS MENTIONED FOR EYE DISEASES (KAN NOIGAL) IN SELECTED SIDDHA LITERATURES

BACKGROUND: Eye care and treatment has been given much prominence in the siddha system of medicine from time immemorial. The types of eye disorders, their etiology, treatment, and preventive measures to protect the eye from the diseases are all mentioned in various siddha literatures. OBJECTIVE: To provide an updated overview of medicines used for eye diseases in siddha literatures and to list out the medications used in eye diseases. MATERIALS AND METHODS: The data about eye diseases and its treatment are collected from Government Siddha Medical College Library, Central Council for Research in Siddha Library, Anna Centenary Library, Siddha literatures such as Agathiyar Nayana vidhi, Nagamuni Nayana vidhi, Sarabendra vaithiya muraigal (Nayana Roga Chigichai), Anuboga Vaithiya Navaneedham, Kannusamy Parambarai Vaithiyam etc and also the information about eye diseases is collected from the articles published in reputed journals such as Pubmed and google scholar. RESULT: The study will be useful for standardization and evaluation of eye medications and beneficial for future generations to carry out the research process on new eye medications. CONCLUSION: There is a need to understand the importance of routine eye care because any impairment in our vision leads to dependency on others. So I conclude that this study will be beneficial for routine eye care and the research process on new eye medications.

In vitro cytotoxicity and apoptosis inducing effect of Vallarai Kirutham with Rasa Parpam in HeLa Cell Lines

Cancer is an uncontrolled proliferation of cells that can affect nearby tissues as well as distant organs. Cervical cancer is predicted to be among the four most prevalent cancers in women overall in 2020, with 342,000 mortality and 604,000 new cases. Around 6-29% of all cancers in women in India are caused by cervical cancer. Women who have had several sexual partners, recurrent abortions, or vaginal deliveries—all of which cause repetitive stress to the cervix are more likely to develop cervical cancer. Radiation, chemotherapy, and surgery are being used to treat cancer. All of these treatments come with side effects, which can range in severity and have a serious psychological impact on patients. The Siddha system has a number of accessible herbo mineral formulations for treating cervical cancer. According to Siddha literature (Agasthiyar Vaidhya Rathina Churukkam, 360), the treatment of cervical cancer (yoni puttru) is advised for the Siddha medicine Vallarai Kirutham with Rasa parpam. The goal of this research was to find out this formulation's potential anti-cancer properties using MTT assays on HeLa cell lines. These findings of the current study suggest that the MTT technique's lowest reading for cell viability was 1.26±0.009% at a concentration of 100 µl/ml. This was followed by concentrations of 75 µl/ml, 50µl/ml and 25µl/ml which showed 7.91±0.004%, 23.91 ±0.01% and 37.17± 0.01% similarly, 10 µl/ml shows 52.09±0.01%.The matching IC50 value was discovered to be 12.44% and AO/EB dual staining was used to examine apoptotic activity. The outcomes of in-vitro experiments using the HeLa cell line. According to the outcome of in vitro experiments done on the HeLa cell line, the drug had substantial anti-cancer and therapeutic value in the management of cervical tumours .With a variety of time tested medications the Siddha system has shown outcomes that were clinically important for the management of cervical carcinoma. One of those medications has to undergo additional testing in patients with cervical cancer through clinical research.

A drug review on potency of Azhingil Vithai Thailam, Siddha Polyherbo formulation in the management of Ceganavatham

In Siddha system of medicine the diseases are categorized and concluded as 4448 diseases. Among these classification there are Vatha disease, Pitha diseases and Kabha diseases. The common Vatha disease are Thandaga Vatham(Lumbar spondylosis), Vathasthambam (Sciatica) and Cegana Vatham (Cervical Spondylosis. Cervical spondylosis is a disorder characterised by increasing degeneration of the intervertebral disc, with subsequent changes in the bones and soft tissues Symptoms are usually a manifestation of encroachment on local neural elements. The symptoms and signs appear to be related to the cause and time course of compression as well as the structures being compressed. Many formulations been indicated for the Cervical spondylosis, and one among these is Azhingil vithai Thailam. Every drug in the Azhingil vithai thailam possess the Anti- Vatha Property, hence this article provides the efficacy of Azhingil vithai Thailam on Cervical Spondylosis based on the Siddha literature and Modern science.

In vitro Evaluation of Anticancer Activity of Karanthai Legium (Kl) – A Siddha Medicine for Cervical Carcinoma against Hela Cell Line By MTT Assay

Cervical cancer become a major global burden and of course considered one of the leading causes of cancer-related deaths in women, particularly in developing countries. Viral induction of human papilloma viruses (HPV) can disturb the basic cellular mechanism of growth control. Currently medicinal plants have become the source of drug discovery in research for treating cancer. Siddha medicine is an ancient medical system of India which classifies diseases into 4448 types. In Siddha literature, Cancer is mentioned as Vippuruthinoipadalam (Carcinoma like illness), Kiranthi noi, Mega Katti, Pilavai and Putru noi (tumour). Literary evidences have a classic collection of herbal, mineral, herbo- mineral medicines for Cancer. Many anti-cancerous drugs are now in practice in Siddha medicine. Among them, Karanthai Legium (KL) a classical herbo mineral drug having anticancer potential is mentioned in Siddha Literature. The main objective of this study is to evaluate anti- cancer activity against HeLa cell using MTT assay. Present study revealed that, Karanthailegium (KL) at high as 200µg/ml showed high cytotoxicity activity as high as 72.44% against HeLa cell lines. IC50 of the tested KL against HeLa cells was calculated as104.90µg/ml. It was concluded from the result of the present study that the formulation KL possesses promising anti-cancer activity.

Different medicinal herbs for madhumegam (diabetes mellitus) prescribed in selected classical siddha literature: A review

Siddha system of medicine, one of the ancient medical systems has the great potential of medicinal resources as repository since ages. Diabetes is a major lifestyle disorder, the prevalence of which is increasing globally. Diabetes mellitus is compared with Madhumegam in Siddha literature. Most of the contemporary drugs work on insulin metabolism and several metabolic pathways to reduce hyperglycaemic conditions. The conventional Siddha drugs works on the same platform through its basic principles. Siddha medicinal plants which are having hypoglycaemic activity by involving various metabolic pathways are taken in to account and considered for a review. This process will create some new ideas in the avenue of research.

Standardization and antimicrobial studies of Venkara neer

Standardization of mineral formulation is essential in order to assess the quality of drugs. The present paper reports preparation and standardization of a mineral formulation which contains borax. This Siddha formulation is used for washing external wounds mentioned in the Siddha literature. The aim of this study to estimate quality of Venkaraneer by conducting physicochemical analysis, HPTLC ,heavy metal analysis, test for specific pathogens and also antimicrobial activity.from this result the siddha drug Venkaraneer has standard quality and have antimicrobial potency against bacterial pathogens.

Standardization of Anda Neer prepared as per Siddha Literature

Siddha drugs are natural products obtained from herbal, mineral and animal kingdom. Most of the Siddha medicines are effective but they have not been standardized yet. So, there be in a need to progress a standardization technique. Anda Neer is a classical Siddha preparation for curing redness of eye mentioned in the textbook of Siddhar Aruvai Maruthuvam written by Dr. K. S. Uthamarayan.. The aim of this study to estimate quality of Anda Neer by conducting physico-chemical analysis, and other analytical techniques. This study was done based on Pharmacopoeial laboratory for Indian Medicine guidelines. Other analytical technique like High performance thin layer chromatography was done through CAMAG software. The physical parameters like state, odour, touch, flow Property and appearance/colour revealed that it was liquid, characteristic odour, non-greasy to touch, free flowing, colourless and transparent. So the drug AN is good in nature and safe to use. The pH value of AN was 7 which indicates that the drug is neutral in nature. A more acidic or alkaline pH value induces lacrimation, ocular pain, and discomfort. In the clarity test observation it was found as clear solution with no major visible particles. HPTLC finger print analysis of the extracts showed the presence of possible number of components. The test drug is devoid of microbial contamination, pesticide residues and aflatoxin assay were below quantification limit. From the result we infer that the drug is of standard quality and shall be used as reference in pharmacopoeia standards.

In-Silico Anti-Diarrhoeal Evaluation of Lagu Gangathara Chooranam

Background: In siddha system of medicine diarrhoea can be compared with kazhichal. Diarrhoea results from several factors like intestinal hyper-motility, malabsorbtion of water, inflammation of GIT or use of laxatives. In siddha literature, the preparation named lagu gangathara chooranam is indicated for kazhichal. Aim: The study aims to perform the in-silico computational studies of phytoconstituents of the siddha formulation lagu gangathara chooranam targeted against m3 muscarinic acetyl choline receptor for anti-diarrhoeal activity. Methods: The retrieved phytocomponents were subjected for docking calculations against target enzyme m3 muscarinic acetylcholine receptor. Results: The computational analysis retrived 11 bio-active compounds from herbal formulation. It includes limonene, α-humulene, cyperolone, lupeol, beta amyrin, betulinic acid, mangiferin, quercetin, caryophyllene and luteolin. These compounds significantly bind against the target m3 muscarinic acetylcholine receptor. Hence these phytocompounds may produce significant anti-diarrheal activity by hindering the activity of m3 muscarinic acetylcholine receptor in the intestinal region which mediates the diarrhoea. Conclusion: Further clinical trials or experimental studies could be conducted to confirm the effectiveness of this siddha formulation lagu gangathara chooranam against diarrhoea.

Phytochemical characterization of Siddha herbomineral formulation: Kalingathi Kadugu in the management of Karpa Vippuruthi (cervical cancer)

Abstract Kalingathi Kadugu (KK) is a herbomineral formulation mentioned in Siddha literature “Agathiyar Vaithya Vallathi 600” (AVV 600) used for the management of ascites, abdominal disorders, and cancer. Phytochemical and thin-layer chromatography (TLC) analyses are important methods to identify the fingerprints of the chemical composition of traditional medicines. KK has been prepared as per AVV 600 after following proper purification methods for the ingredients. The phytochemical and TLC analyses were performed as per standard guidelines. Phytochemical analyses revealed the presence of tannins and triterpenoids. The triterpenoids in the formulation have therapeutic potential against the diseases. The results from this analysis will serve as a preliminary standard for KK, which is an important Siddha formulation.

Antibacterial activity of Siddha formulation of Vellampattai koppali kizhalam (mouthwash) against Klebsiella pneumoniae and Streptococcus pyogenes for oral hygiene

Abstract Background: Poor oral health characterized by inadequate hygiene may result in the formation of extensive dental biofilms (plaque) promoting oral colonization of pathogens. Siddha medicine plays a major role in the oral health care. Traditional claims have to be validated through scientific research for wider acceptance. The Siddha literature Anuboga Vaithiya Bhrama Ragaseyam mentioned Vellampattai Koppali Kizhalam (VKK) for oral hygiene and prevention of periodontal disease. Objective: The study aims to evaluate the anti-bacterial activity of VKK to prevent oral infection. Materials and Methods: VKK is an efficient Siddha formulation used to prevent oral infection. One of the important ingredients is Veppam pattai (Azidarchia indica) which is found to have an antibacterial effect. In the present study, the antibacterial susceptibility test for VKK was done using the microdilution plate method using resazurin. Results: The current study observed that VKK exhibited significant antibacterial activity against Streptococcus pyogenes (MIC 1000 µg) and Klebsiella pneumoniae. The color change was observed and recorded as the minimum inhibitory concentration (MIC) value using the standard drug chloramphenicol (10 µg). Conclusion: The study revealed that the Siddha formulation have strong anti-bacterial activity and offers profound therapeutic effects against periodontal disease and improve oral hygiene.