Antibacterial activity of Siddha formulation of Vellampattai koppali kizhalam (mouthwash) against Klebsiella pneumoniae and Streptococcus pyogenes for oral hygiene

Abstract

Abstract Background: Poor oral health characterized by inadequate hygiene may result in the formation of extensive dental biofilms (plaque) promoting oral colonization of pathogens. Siddha medicine plays a major role in the oral health care. Traditional claims have to be validated through scientific research for wider acceptance. The Siddha literature Anuboga Vaithiya Bhrama Ragaseyam mentioned Vellampattai Koppali Kizhalam (VKK) for oral hygiene and prevention of periodontal disease. Objective: The study aims to evaluate the anti-bacterial activity of VKK to prevent oral infection. Materials and Methods: VKK is an efficient Siddha formulation used to prevent oral infection. One of the important ingredients is Veppam pattai (Azidarchia indica) which is found to have an antibacterial effect. In the present study, the antibacterial susceptibility test for VKK was done using the microdilution plate method using resazurin. Results: The current study observed that VKK exhibited significant antibacterial activity against Streptococcus pyogenes (MIC 1000 µg) and Klebsiella pneumoniae. The color change was observed and recorded as the minimum inhibitory concentration (MIC) value using the standard drug chloramphenicol (10 µg). Conclusion: The study revealed that the Siddha formulation have strong anti-bacterial activity and offers profound therapeutic effects against periodontal disease and improve oral hygiene.