A new dammarane-type glycoside and a new long chain sesquiterpene glycoside, along with nine known compounds 20(S)-ginsenoside Rh1 ( 3 ), 20(R)-ginsenoside Rh1 ( 4 ), ginsenoside F1 ( 5 ), amarantholidoside IV ( 6 ), ginsenoside Rc ( 7 ), 20(S)-ginsenoside Rg2 ( 8 ), 20(R)-ginsenoside Rg2 ( 9 ), ginsenoside Rd ( 10 ) and gypenoside XLVI ( 11 ) were isolated from Gynostemma yixingense. The structures of the new compounds were determined on the basis of spectroscopic analysis, including 1D-, 2D-NMR and ESI-MS techniques as well as by comparison of the spectral data with those of related compounds as 2 α,3 β,20(S)-trihydroxydammar-24-ene-3-O-[ β- d-glucopyranosyl((1 → 2)- β- d-glucopyranosyl]-20-O-[ β- d-xylopyranosyl((1 → 6)- β- d-glucopyranoside] ( 1 ) (2E,6E)-10- β- d-glucopyranosyl-1,10,11-trihydroxy-3,7,11-trimethyldodeca-2,6-diene ( 2 ).