Serum Resistin Levels Research Articles

Investigating the Effect of Two Types of Aerobic and Resistance Training during a Ketogenic Diet on the Serum Levels of Adipokines and Insulin Resistance in Overweight or Obese Men

Introduction: The purpose of the present study was to investigate the effect of aerobic and resistance training during a Ketogenic Diet (KD) on serum levels of adipokines and insulin resistance indices in overweight or obese men. Method: A total of 36 overweight or obese men were randomly divided into three groups, namely Aerobic Training during KD (AT-KD), Resistance Training during KD (RT-KD), and KD alone (KD). In addition to following the KD, the training groups performed aerobic or resistance training three days per week for six weeks. For statistical analysis, the mixed two-way analysis of variance test with a significance level of less than 0.05 and SPSS software was used. Results: The serum levels of adiponectin, resistin, and SFRP5 did not differ significantly between the groups. In all groups, resistin had a significant decrease, whereas adiponectin and SFRP5 had a significant increase, compared to the pre-test. The serum levels of glucose, insulin, and triglycerides, as well as insulin resistance and sensitivity indices, were not significantly different between the groups. A significant decrease in glucose and triglyceride levels was observed in the three groups compared to the pre-test. Both KD and AT-KD groups significantly decreased insulin and HOMA-IR and increased McAuley indices compared to the pre-test. Only the AT-KD group significantly increased the QUICKI index compared to the pretest. Conclusion: Both AT-KD and RT-KD improved the level of adipokines in overweight or obese men. However, there was no difference between the two types of training. Despite the lack of difference between the two types of exercise, aerobic training may be more effective than resistance training during a KD

Effect of Lifestyle Interventions during Pregnancy on Maternal Leptin, Resistin and Offspring Weight at Birth and One Year of Life.

Lifestyle during pregnancy impacts the health of the mother and child. However, the extent to which physical activity affects maternal biomarkers and factors that might influence birth weight remains unclear. We analysed data from two lifestyle interventions in which the effects of an exercise programme (2x/week, 60-90 min) on the course of pregnancy with regard to adipokines and offspring were evaluated. A total of 70 women participated in this study (45, intervention group; 25, control group). Anthropometric data and maternal fasting serum leptin and resistin levels were measured at three time points (approximately 14th (T1), 24th (T2), and 36th (T3) weeks of gestation). Neonatal/child data were retrieved from screening examinations. Independent of the intervention, we found a positive correlation between the fat mass at T1 and both leptin and resistin levels at all time points. Leptin level was significantly higher in the control group at T3; however, no differences between the groups were found for resistin. The birth weight was influenced by the birth length, fat mass at T1/T3, and resistin level at T2. The BMI-SDS at one year of age was influenced by maternal fat-free mass at T3 and resistin at T1/T2. Even if these results can only be interpreted cautiously, lifestyle interventions during pregnancy are important in promoting maternal and child health. Further randomised controlled trials and translational studies are warranted to clarify the underlying mechanisms.

Yüksek Yağlı Diyetle Beslenen Ratlarda Egzersizin Serum Resistin ve Leptin Değerlerine Etkisi

Aim: This study was performed to investigate the effect of exercise on serum resistin and leptin values in rats fed with a high-fat diet. 
 Material and Method: 24 Wistar albino male rats were used in the study. They were randomly divided into 4 groups, with 6 rats in each group. The groups were determined as the control group (C), exercise group (E), high-fat diet (HFD) group, and high-fat diet + exercise (HFDE) Group. 
 Results: When the findings obtained in this study were evaluated statistically, it was determined that the resistin values were similar in the C, E and HFDE groups, and higher in the HFD group compared to the other groups. It was observed that resistin value increased with high-fat diet and decreased with exercise. When evaluated in terms of leptin levels, the C and E groups showed similarity, while the HFD and HFDE groups showed similarity with each other. Although exercise decreased the leptin level, which was highly increased with a high-fat diet, it was not statistically significant.
 Conclusion: It has been determined that feeding with a high-fat diet causes an increase in serum resistin and leptin levels, and exercise provides a significant decrease in resistin values, but is not effective in leptin levels.

Adiponectin, Leptin, and Resistin Are Dysregulated in Patients Infected by SARS-CoV-2.

Obesity, through adipose tissue (AT) inflammation and dysregulation, represents a critical factor for COVID-19; here, we investigated whether serum levels of adiponectin, HMW oligomers, leptin, and resistin are modulated and/or correlated with clinical and biochemical parameters of severe COVID-19 patients. This study included 62 severe COVID-19 patients; 62 age and sex-matched healthy subjects were recruited as a control group. Anthropometric and biochemical parameters were obtained and compared. Adiponectin, HMW oligomers, leptin, and resistin were analyzed by ELISA. The adiponectin oligomerization state was visualized by Western blotting. When compared to healthy subjects, total adiponectin levels were statistically lower in severe COVID-19 while, in contrast, the levels of leptin and resistin were statistically higher. Interestingly, HMW adiponectin oligomers negatively correlated with leptin and were positively associated with LUS scores. Resistin showed a positive association with IL-6, IL-2R, and KL-6. Our data strongly support that adipose tissue might play a functional role in COVID-19. Although it needs to be confirmed in larger cohorts, adiponectin HMW oligomers might represent a laboratory resource to predict patient seriousness. Whether adipokines can be integrated as a potential additional tool in the evolving landscape of biomarkers for the COVID-19 disease is still a matter of debate. Other studies are needed to understand the molecular mechanisms behind adipokine's involvement in COVID-19.

Fat fighting liraglutide based nano-formulation to reverse obesity: Design, development and animal trials

Obesity is a metabolic disease, which is one of the major causes of morbidity and mortality, where therapeutic options are limited. Treatment of obesity is necessary as it is associated with fatal complications like diabetes mellitus, cardiovascular disease, non-alcoholic fatty liver disease, osteoarthritis, and many more. Liraglutide (Lir), a synthetic analogue of Glucagon-like Peptide-1 (GLP-1), is the FDA approved anti-obesity drug, however, its major limitation is its clinical application which needs frequent parenteral injections. To address the issue of regular injection, we have synthesized a fat fighting oral nano-formulation of liraglutide with a sustained release feature, which was evaluated against high fat diet (HFD) induced obesity in mice. Experimental obesity was induced in mice by feeding HFD for 26 weeks. Lir nanoparticles (NP) were fabricated with chitosan via ion-gelation technique and were coated with Eudragit@S100 to protect the drug in harsh gastric conditions. Physiochemical characterization of Eu-Lir-Cs-NP demonstrated a small particle size of 253.1 ± 1.21 nm with ∼ 9.74 % loading and ∼ 72.11 % encapsulation efficiency of the drug. In-vitro studies showed successful cellular uptake of NP in Caco-2 cells and were stable in various enteric fluid pH conditions. Eudragit@S100 coated chitosan NP were able to protect the drug from harsh gastric pH conditions with more than ∼ 74% of recovery. Treatment of two weeks of liraglutide Eu-Lir-Cs-NP (0.1, 0.2 and 0.4 mg/kg, orally; twice daily) moderately reduces obesity in mice as evidenced by a reduction in the body weight, blood glucose, serum total cholesterol, serum triglyceride, serum resistin and serum insulin level of mice. In addition, significant reduction of liver weight, abdominal white adipose tissue, and hepatic oxidative stress were noted. Our results suggest that chitosan-based NP of liraglutide can be an effective and convenient formulation for the management of obesity.

Assessment of Serum Resistin: A Recently Identified Adipocyte-Derived Hormone in Obese and Lean Individuals

Background Due to the alarming rate at which obesity is increasing a global epidemic has been declared. Resistin one of the rodents cysteine-rich protein classes suggests that these adipocytokines could contribute to the molecular causes of obesity. Our ability to grasp this concept could be crucial in the development of novel therapies for conditions associated with obesity.Aim To investigate the connection between obese participants serum resistin levels and body mass index BMIMethods Using an ELISA kit the serum resistin levels of 15 obese persons aged 25plusmn10 years with a BMI score of 30plusmn5 and 15 lean individuals in the same age group with a BMI score of 20plusmn2 were investigated. Evaluations were also conducted on serum glucose and lipid profile markers such as cholesterol low-density lipoproteins LDL and high-density lipoproteins HDL.Results No statistical significance in serum resistin levels was noted between obese and lean individuals. Serum resistin showed a positive correlation 0.11 with HDL but yielded a negative correlation -0.263 with LDL Very-low-density lipoprotein VLDL and BMI. Independent Samples Test for HDL LDL VLDL and BMI between obese and lean showed a very high statistical significance p lt0.0001. Though serum resistin levels remained high in obese it was not significant p-value 0.078.Conclusion Although an overt rise in obesity could not be seen the concentration of circulating resistin in obese people revealed a correlation with HDL and anthropometric factors. In summary the precise role that human resistin plays in obesity insulin resistance and the onset of diabetes is still up for debate. In the near future the precise role of the intriguing new hormone resistin in metabolism will become clear.

Relationship of resistin gene polymorphism (rs7139228) with resistin levels and metabolic syndrome risk in obese subjects.

Background: despite the relationship of resistin with metabolic syndrome (MS), the relationship of the 5'UTR intron C/T variant SNP rs7139228 of the RETN gene with the presence of MS has not been evaluated. Objective: the objective of this study is to evaluate the influence of SNP rs7139228 of the RETN gene on circulating resistin levels, as well as on MS in obese subjects. Material and Methods: a Caucasian population of 1003 obese subjects was enrolled. An anthropometric evaluation (weight, waist circumference, fat mass), evaluation of nutritional intake, biochemical study (glucose, insulin, C-reactive protein, lipid profile, insulin, HOMA-IR, resistin) and rs7139228 genotype was carried out. Results: genotype distribution was: 852 subjects with GG (84.9 %), 147 subjects with GA (14.7 %) and 4 subjects with AA (0.4 %). The allelic frequency was G (0.92) and A (0.08). Serum levels of resistin (delta: 1.7 ± 0.2 ng/ml; p = 0.01), insulin (delta: 4.2 ± 0.4 IU/L; p = 0.01) and HOMA-IR (delta: 1.9 ± 0.2 units; p = 0.03) were higher in patients carrying the A allele than in non-carriers. The overall prevalence of MS was 48.1 %. A logistic regression analysis showed a high percentage of hyperglycemia (OR = 1.60, 95 % CI = 1.08-2.96; p = 0.02) and metabolic syndrome (OR = 1.33, 95 % CI = 1.07-3.39, p = 0.02) in carriers of the A allele after adjusting for resistin levels, sex, BMI and age. Conclusions: the A allele of the genetic variant rs7139228 is associated with higher levels of resistin, basal insulin, insulin resistance, and prevalence of metabolic syndrome in obese subjects.

Rhinacanthin C Ameliorates Insulin Resistance and Lipid Accumulation in NAFLD Mice via the AMPK/SIRT1 and SREBP-1c/FAS/ACC Signaling Pathways.

Rhinacanthin C (RC) is a naphthoquinone ester with an anti-inflammatory activity extracted from Rhinacanthus nasutus (L.) Kurz (Rn). It has been proven to improve hyperglycemia and hyperlipidemia, but the prevention and mechanism of RC in nonalcoholic fatty liver disease (NAFLD) are not clear. In the current study, we first extracted RC from Rn using ethyl acetate and identified it by HPLC, MS, and NMR. At the same time, molecular docking analysis of RC with AMPK and SREBP-1c was performed using AutoDock software. In addition, the mouse model of NAFLD was induced by a high-fat diet in vivo, and low, medium, and high concentrations of RC were used for intervention. The results showed that RC significantly reduced the body mass and liver body coefficient of NAFLD mice, inhibited liver inflammation and fat accumulation, and improved insulin resistance. Further studies showed that RC significantly reduced the levels of serum leptin and resistin, upregulated the expression levels of adiponectin and adiponectin receptor in the liver, and inhibited the expression levels of MCP-1, TNF-α, and IL-6. In terms of mechanism, RC upregulates the expression of p-AMPK and SIRT1 and downregulates the expression of p-p65, SREBP-1c, Fas, Acc-α, PPAR-γ, and SCD1. These studies suggest that RC improves insulin resistance and lipid accumulation in NAFLD by activating the AMPK/SIRT1 and SREBP-1c/Fas/ACC pathways, respectively.

Correlation between LRG1 and Adipokines in Psoriasis

Background and Objective. Patients with psoriasis may exhibit abnormal changes in serum adipokine levels, which are often related to disease severity of the disease. Leucine‐rich alpha‐2‐glycoprotein 1 (LRG1) is an acute‐phase inflammatory protein that may be linked to adipokines in psoriasis. In this study, we evaluated the differences in the expression of adipokines and LRG1 between patients with psoriasis and healthy individuals, analyzed the correlation between the expression of LRG1 and adipokines, and explored their relationship with psoriatic lesions. Methods. In this cross‐sectional study, patients with psoriasis (n = 54) and healthy controls (n = 26) were enrolled, and their clinical characteristics were recorded. Fasting venous blood samples were collected from each participant. The serum concentrations of leptin, resistin, adiponectin, and LRG1 in each sample were measured using the enzyme‐linked immunosorbent assay. Results. The study included 54 patients with psoriasis vulgaris and 26 healthy controls. The serum levels of LRG1, leptin, and resistin were significantly higher in patients with psoriasis than in healthy controls. Conversely, adiponectin levels were significantly lower in patients with psoriasis. The study showed that LRG1 expression was positively correlated with leptin and resistin expression but negatively correlated with adiponectin expression. Interestingly, only leptin, resistin, and LRG1 expression showed a linear correlation with the Psoriasis Area and Severity Index (PASI). When we categorized patients with psoriasis based on their LRG1 levels, we observed that the group with high LRG1 levels showed a higher PASI. Conclusions. We observed a significant correlation between LRG1 and adipokine expression in patients with psoriasis. In addition, the expression levels of LRG1, leptin, and resistin were observed to be correlated with the severity of psoriasis. We believe that the occurrence and development of psoriasis are collectively influenced by LRG1 and leptin/resistin expression.

The effects of sleep deprivation on insulin, resistin and visfatin levels in healthy humans

The Effects of Sleep Deprivation on Insulin, Resistin and Visfatin Levels in Healthy Humans ABSTRACT Background: Sleep deprivation is known to affect circulating insulin and glucose levels which in turn modulate glucose metabolism. However, the mechanism of alterations in glucose homeostasis during sleep deprivation is not known. In this study, we investigated circulating resistin and visfatin levels in response to 40 hours of sleep loss in order to shed light on the above-mentioned mechanism. Methods: This study included 12 healthy young adult subjects (aged between 18-32 years). All participants underwent polysomnographic evaluation and oral glucose tolerance test and then fasting venous blood samples were collected in morning hours. Then, subjects remained awake for 40 hours under actigraphic monitorization. At the end of sleep deprivation, blood samples were collected again. Serum insulin, resistin and visfatin levels were measured in all blood samples. Insulin was determined by chemical immune assay method, whereas resistin and visfatin levels assayed by ELISA. Results: Compared to baseline, 40-hour total sleep deprivation resulted in a significant increase in serum insulin levels (10.75±7.75 vs 35.98±27.96 IU; p=0.002) and a significant decrease in resistin levels (21.94±7.65 vs 11.71±5.31 IU; p=0.002). Visfatin levels remained unchanged (6.29±3.31 vs 5.43±5.08 IU; p>0.05). Conclusion: These results suggested that short-term total sleep deprivation may lead to insulin resistance which was evidenced by a significant increase insulin levels independent of resistin. This may contribute to pathophysiology of type 2 diabetes mellitus under conditions of chronic sleep deprivation.

The role of resistin gene polymorphism in Iraqi breast cancer patients

Introduction and Aim: Breast cancer is a complex disease and one of the most common cancers among women. Breast cancer is linked to a number of inherited gene alterations which could either initiate breast tumors or be involved in the multistage process of breast cancer development. In this study we aimed to genotype the resistin (RETN) gene linked to breast cancer and determine the effects of RETN single nucleotide polymorphisms (rs3219175, rs34788323) to breast cancer in Iraqi women.
 
 Materials and Methods: This study included 70 (35 malignant and 35 benign) patients with breast cancer and 35 breast-cancer free Iraqi women. Blood samples were collected from all women and subjected to two RETN SNPs (rs3219175, rs34788323) genotyping and determining their allelic frequencies. The serum resistin concentration was determined for each sample. Data was subjected to statistical analysis and p-value determined.
 
 Results: A statistically significant difference for the GA genotype in SNP rs3219175 and TT genotype in SNP (rs34788323) was seen among breast cancer patients, and controls. among Iraqi women. The mean resistin concentration was substantially greater in the malignant group (4,194 ng/ml) compared to the benign group (1,651 ng/ml) and the control group (1,476 ng/ml) (P 0.01). The rs34788323 polymorphism's CC genotype could be associated with higher resistin levels. The blood resistin level was substantially greater in breast cancer patients with the AA genotype (7.710 0.57ng/ml) than in benign (2.340 0.24ng/ml) or control group (2.231 0.15ng/ml), although the difference was not statistically significant, according to rs3219175 SNP results. The incidence of breast cancer was 42.85% higher in the middle age group (40-49 years). The study did show a detrimental relationship between BMI and tumour size, stage, and grade.
 
 Conclusion: Results indicate a correlation between retn gene polymorphism, resistin serum levels and breast cancer susceptibility.

Assessment of Serum Omentin-1, Adipsin and Resistin Levels in Beta Thalassemia Male Patients in Baghdad City of Iraq

Adipocytokines has a significant impact on the development of many illnesses. (e.g. beta thalassemia), This research aims to determine the levels of Omentin-1, adipsin and resistin of (35) male patients their age (20–30) years that effected with Beta-Thalassemia city and (15) of healthy people as control group in Baghdad city by using enzyme linked immuonsorbant assay (ELISA). The findings of this research revealed a significant increase (p ≤ 0.01) in levels omentin-1, which are (78.33 ± 6.14) pg/mL in patients group and (35.88 ± 5.8) ) pg/mL in control group. The levels of adipsin show a significant increase (p ≤ 0.01), which are (603.13 ± 11.46) ng/mL in patients group and (171.76 ± 17.45) ng/mL in patients group in control group, also a significant increase (p ≤ 0.01) in the levels of resistin which are (2.1 ± 0.5) ng/mL and (0.35 ± 0.06) ng/mL in control group.

Anti-Obesity Effect of Chitoglucan in High-Fat-Induced Obesity Mice.

Chitoglucan (CG) is a bioactive component obtained from Flammulina velutipes Sing, an edible mushroom, which is known to have an anti-obesity effect. However, its biological and hormonal activities in alleviating obesity through regulation of adipocyte-derived proteins have not been examined yet. The present study aimed to investigate the anti-obesity effects of chitoglucan and its hormonal mechanisms in high-fat diet (HFD)-induced mice. The mice were fed either a normal diet (Normal group) or a high fat diet (HFD group) over 6 weeks. The HFD fed mice were administered with saline (HFD group), adipex (HFD + adipex group), chitoglucan 50, 150, or 300 mg/kg/day for 3 weeks (HFD + CG groups). The food consumption, body weight, fat contents, and the levels of serum leptin and resistin were assessed after treatment of chitoglucan. the HFD produced a marked increase in body and fat weights after 6 weeks of feeding compared with the Normal group. Administration of chitoglucan for 3 weeks tended to reduce body weight and significantly decreased parametrical adipose tissues in HFD groups. The level of serum leptin in the HFD group was markedly higher than that in the Normal group, whereas the level of leptin in the chitoglucan treated groups was significantly decreased in comparison with the HFD group. In addition, the level of serum resistin in high-fat diet group tended to be more increased than Normal group. However, the serum resistin level was significantly reduced in HF diet groups after treatment with chitoglucan (50 mg/kg or 150 mg/kg). Collectively, these data suggest that chitoglucan from the Flammulina velutipes may be useful in the treatment of high diet-induced obesity and metabolic syndrome.

Evaluation of Reactive Oxygen Metabolites, Resistin, and Red Complex Bacteria in Obese Subjects with or without Periodontitis

The study's aim was to assess and compare the clinical parameters, plasma reactive oxygen metabolites (ROM) levels, gingival crevicular fluid (GCF) resistin, serum resistin values, and red complex bacteria in obese or overweight subjects with and without periodontitis and also to determine the effect of non-surgical periodontal therapy (NSPT) on plasma ROM, serum, and GCF resistin values in obese or overweight subjects with chronic periodontitis. A total of 160 subjects were recruited and designated into four groups with 40 subjects each as group I - obese with chronic periodontitis; group II - normal weight subjects with chronic periodontitis; group III - obese subjects with healthy periodontium; and group IV - normal weight subjects with healthy periodontium. The periodontal parameters, plasma ROM, GCF resistin and serum resistin, and red complex bacteria levels were estimated at baseline. After baseline assessment, scaling and root planing (SRP) were done in the patients of groups I and II. Two months after the completion of SRP, clinical parameters such as plaque index (PI), probing pocket depth (PPD), gingival index (GI), and clinical attachment loss (CAL), plasma ROM levels, serum resistin, and GCF resistin levels were analyzed. An increase in plasma ROM, GCF resistin, and red complex bacteria levels was observed in obese subjects with periodontal disease and the increase was noted in obese subjects with healthy periodontium. Comparing plasma ROM, GCF resistin values between groups I and II, 2 months after SRP, a decrease in these levels were observed in group II. Our study results depict that obesity can be considered as a risk indicator for periodontal disease. Obesity has a negative impact on both general health and oral health. Promoting appropriate physical activity, healthy eating behavior, and oral hygiene practice are fundamental elements of the prevention of both obesity and periodontal disease.

Study of the level of leptin and resistin and some biochemical tests for people using ketogenic diet in Kirkuk city

Recently, the keto diet has been relied upon to lose weight, but there are some advantages and disadvantages that could be diagnosed through the current study. The study included 50 individual (male and female) and 25 Control, collected Blood samples for determination of leptin and resistin and Lipid profile. The results showed that the serum level of leptin and resistin were significantly lower in Diet group (2.137 g/l, 4.34 g/l) respectively as compared to healthy control group (leptin; 3.52 g/l, resistin; 4.739g/l) respectively. Lipids profile levels was significantly higher in Ketogenic Diet group, were the results of each (cholestrol -TC, low density lipoprotein-LDL-c, triglycerides -TG) (298 g/dl, 169.9 g/dl, 292.3 g/dl) respectively as compared to healthy control group (159.4 g/dl, 85.4 g/dl, 191 g/dl), while the result of High density lipoprotein (HDL-c) showed lower significantly in Diet group (31.9 g/dl) compared with control group (52.4 g/dl). In conclusion, the results of this study showed following the ketogenic diet increases the level of cholesterol and triglycerides (TG) and causes a reduction in albumin, globulin and total protein levels. The results have also showed an increase in adipokin level and a decrease in leptin level. Whereas, no-significant changes were noticed in both resisten and interlukin-6 levels.

Moringa oleifera Improves MAFLD by Inducing Epigenetic Modifications.

Background and aims. Metabolic Associated Fatty Liver Disease (MAFLD) encompasses a spectrum of diseases from simple steatosis to nonalcoholic steatohepatitis (NASH). Here, we investigated the hepatoprotective role of Moringa oleifera aqueous extract on hepatic miRNAs, genes and protein expression, as well as histological and biochemical parameters in an experimental model of NASH. Methods. Male C57BL/6J mice were fed with a high fat diet (HFD, 60% lipids, 42 gr/L sugar in water) for 16 weeks. Moringa extract was administered via gavage during the final 8 weeks. Insulin Tolerance Test (ITT) and HOMA-IR were calculated. Serum levels of insulin, resistin, leptin and PAI-1 and hepatic expression of miR-21a-5p, miR-103-3p, miR-122-5p, miR-34a-5p and SIRT1, AMPKα and SREBP1c protein were evaluated. Alpha-SMA immunohistochemistry and hematoxylin-eosin, Masson’s trichrome and sirius red staining were made. Hepatic transcriptome was analyzed using microarrays. Results. Animals treated with Moringa extract improved ITT and decreased SREBP1c hepatic protein, while SIRT1 increased. Hepatic expression of miR-21a-5p, miR-103-3p and miR-122-5p, miR34a-5p was downregulated. Hepatic histologic analysis showed in Moringa group (HF + MO) a significant decrease in inflammatory nodules, macro steatosis, fibrosis, collagen and αSMA reactivity. Analysis of hepatic transcriptome showed down expression of mRNAs implicated in DNA response to damage, endoplasmic reticulum stress, lipid biosynthesis and insulin resistance. Moringa reduced insulin resistance, de novo lipogenesis, hepatic inflammation and ER stress. Conclusions. Moringa prevented progression of liver damage in a model of NASH and improved biochemical, histological and hepatic expression of genes and miRNAs implicated in MAFLD/NASH development.

Serum Resistin Level as a Novel Marker of Disease Activity in Patients with Ulcerative Colitis

Serum Resistin Level as a Novel Marker of Disease Activity in Patients with Ulcerative Colitis

Can soy isoflavones in combination with soy protein change serum concentration of adiponectin and resistin? A systematic review and meta-analysis on randomized clinical trials.

Some studies proposed the anti-inflammatory effect of soy protein and soy isoflavones by changing the serum adiponectin and resistin levels. The purpose of this research was to determine the impact of soy isoflavones and soy protein on blood adiponectin and resistin levels in adults. Scopus, PubMed, Cochrane Library, ISI Web of Science, and ClinicalTrials.gov databases were searched until April 2022. The effect size was computed by the mean changes from the beginning for intervention and comparison groups and their standard deviation. In the case of significant heterogeneity, DerSimonian and Laird random-effects model was used. Six and five clinical trials were selected for the systematic review and meta-analysis, respectively. The overall estimate indicated that soy isoflavones in combination with soy protein did not significantly change serum adiponectin level (weighted mean differences (WMD)=0.36 μg/ml; 95% confidence interval (CI): -0.26, 0.99; p= .25), but significantly increased serum resistin level (WMD=0.64 ng/ml, 95% CI: 0.25, 1.04; p= .001). In combination with soy protein, soy isoflavones nonsignificantly increased serum adiponectin levels, but significantly increased resistin levels. New intervention studies with a high dose of soy isoflavones and soy protein in different parts of the world and an updated meta-analysis are needed to confirm the results of our study.

Effect of short-term high fat diet on resistin levels and expression of autophagy-related genes in the cartilage of male rats

Obesity is a significant risk factor for the development of knee osteoarthritis (KOA). However, the precise molecular mechanisms linking obesity to OA remain unclear. In the present study, we investigated the effect of short-term high-fat diet (HFD) on the development of OA and the possible role of the adipokine resistin and autophagy-related genes in mediating this effect. Thirty adult male Wistar rats were equally divided into 2 groups: control and obese groups. Body mass index (BMI), levels of lipid profile, glucose, insulin and HOMA-IR index were significantly higher in the obese group compared with control. Our results revealed significantly higher serum and cartilage resistin levels with a significant increase in the mRNA expressions of toll-like receptor 4 (TLR4), matrix metalloproteinase-9 (MMP-9) and interleukin-1β (IL-1β) as well as protein levels of IL-1β, matrix metalloproteinase-13 (MMP-13), ADAMTS 5 (aggrecanase-2) and caspase-3 in the cartilage of obese rats. The HFD induced a significant upregulation of autophagy related 5 (ATG5), beclin-1 and light chain 3 (LC3) mRNA expressions and a significant downregulation of mammalian target of rapamycin (mTOR) expression in cartilage. The protein levels of cartilage ATG5 were also significantly elevated in HFD-fed group. In conclusion, we suggested that increased levels of resistin and expression of autophagy-related genes may contribute in part, to OA development in HFD-fed rats. This provides a novel insight into the early molecular changes in the cartilage associated with obesity.

Assessment of Serum Resistin and Plasma Calprotectin Levels as Biomarkers of Inflammation in Patients with Familial Mediterranean Fever Disease.

While several inflammatory markers are known to increase in familial Mediterranean fever (FMF) disease cases, the need remains for diagnostic tests specific for FMF that monitor inflammatory activity. We aimed to investigate resistin and calprotectin levels during both attack and attack-free periods of FMF disease and evaluate their use as novel biomarkers of inflammation in patients with FMF. This cross-sectional study included 68 male patients diagnosed with FMF and 20 healthy individuals as controls. Blood samples were obtained from the patients in attack-free periods (at least 15 days after the last attack) and attack periods (in the first 24 hours). Serum resistin and plasma calprotectin levels was measured by ELISA method. Resistin and calprotectin levels were significantly higher in patients during both attack (p =0.001, p <0.001) and attack-free periods (p =0.017, p =0.01) compared to the control group. Logistic regression analysis indicated that resistin levels were predictive for the diagnosis of FMF disease (OR: 1.21; 95% CI: 1.04-1.42; p =0.016). Resistin and calprotectin levels significantly correlated with C-reactive protein, erythrocyte sedimentation rate, fibrinogen, and white blood cells (0.301≤ r ≤ 0.505, p <0.05). Resistin and calprotectin levels were significantly higher in patients than controls, and resistin was predictive for monitoring inflammatory activity in patients with FMF.