Sensitivity Pattern Of Bacterial Isolates Research Articles

Evaluation of the Types and Drug Sensitivity Patterns of Bacterial Isolates from Patients with Eye Discharge at Abia State University Teaching Hospital, Aba, Nigeria

This study aimed to evaluate the types and drug sensitivity patterns of bacterial isolates from patients with eye discharge at the Abia State University Teaching Hospital (ABSUTH), Aba, Nigeria. A total of 100 samples were collected, with the most prevalent bacterial isolates being Staphylococcus aureus (26%) and Coagulase-negative Staphylococci (21%), while no bacterial growth was reported in 42% of samples. In terms of patient demographics, a significant proportion of the patients were females (64%) aged between 61-70 years. In relation to contact lens use, a high percentage (97%) of the bacterial isolates were detected in patients who did not use contact lenses. An occupational distribution analysis revealed that bacterial isolates were more frequent in farmers and traders compared to students and retirees. Antimicrobial sensitivity testing of the bacterial isolates showed varying degrees of resistance, with significant resistance observed against Erythromycin, Ciprofloxacin, and Perfloxacin. In contrast, higher sensitivity was noted towards Chloramphenicol and Gentamycin. The findings underscore the importance of regular microbiological evaluation and sensitivity testing in patients presenting with eye discharges to guide effective antimicrobial therapy. These findings could guide the selection of empiric antibiotics for treating ocular infections in this region and suggest the need for routine microbiological surveillance to monitor and guide antibiotic prescription to mitigate against antibiotic resistance.

Comparative study of antibiotic sensitivity pattern of bacterial isolates from ocular infections among patients with and without diabetes mellitus

Antibiotic sensitivity pattern of bacterial isolates varies from place to place and time to time. This study is aimed at determining the sensitivity patterns in our setup. In the era of emerging resistance to antibiotics, there is a need to determine the antibiotic sensitivity pattern of bacterial isolates from ocular infections and to compare the antibiotic sensitivity pattern of bacterial isolates in ocular infections among patients with and without diabetes mellitus. 1. To determine the antibiotic sensitivity pattern of bacterial isolates in ocular infections. 2. To compare the antibiotic sensitivity pattern of bacterial isolates from ocular infections among patients with and without diabetes mellitus. 3. To study the predisposing factors for development of the multidrug resistant bacteria in ocular infections.: It was a hospital based cross-sectional study carried out among the patients presenting to Outpatient department of Hassan Institute of Medical Sciences, Hassan, Karnataka from January 2022 to June 2022. The aims and objectives of the intended study were explained to the subjects and informed written consent was taken. Institutional ethical clearance was obtained. Data was collected as per the proforma sheet. 110 samples were divided into two groups- Diabetics (Group A) & Non diabetics (Group B). Majority70(63.6%) of samples sent were from ocular pathology Chronic dacryocystitis. Positivity rate of cultures was 31.8%. Most common isolate in both groups was Staphylococcus aureus. 89% of positive culture patients had history of antibiotic abuse. Highest sensitivity was seen with aminoglycosides and resistance was seen with macrolide like azithromycin and fluroquinolone like ciprofloxacin. Antibiotic sensitivity and resistance patterns of bacterial isolates in ocular infections was similar in both Diabetics (Group A) and Non diabetics (Group B). Highest sensitivity was seen for Amikacin, Gentamicin and Tobramycin in both groups. Highest resistance was seen for Azithromycin, Amoxicillin clavulanic acid, Ciprofloxacin in both groups.Multidrug resistance was due to previous history of use of antibiotics, injudicious use of antibiotics for viral, allergic and other conditions, incomplete treatment for ocular infections and extended duration of antibiotic usage.

Study of Antimicrobial Resistance among Enterococcus Species in Tertiary Care Teaching Hospital

The current study was carried out to isolate and identify Enterococcus species from clinical samples and to analyse the antimicrobial susceptibility pattern of Enterococcus Species. All clinical samples which were received in Microbiology laboratory were included in the study. A total of 2958 samples were received. These 129 Enterococci isolates were obtained over a period of 1 year from various samples namely, urine, blood, pus. Samples were processed for microscopy and culture as per the standard guidelines. all samples were inoculated on MacConkey agar and Blood agar. Identification and antibiotic sensitivity pattern of bacterial isolates were carried out by automated system. i.e. VITEK 2 Compact. Out of 2958 samples, Total 129 samples showed growth of enterococci. Out of 129 isolates, 84 were Enterococcus spp, 18 were E.faecalis, 16 were E.faceium. Out of total 129 samples, 76 samples were of males and 53 were of females. Most common samples from which enterococci were isolated was urine accounting for 85 followed by Pus accounting for 15. Medicine department showed the highest isolates with 88. Out of 129 Enterococci 64 showed resistance to primary line of drug like Benzyl penicillin, 79 Enterococci showed resistance to Ampicillin. Out of 129 enterococci 70 showed resistance to secondary line of drug like tetracycline,59 isolates showed resistance to High level Gentamycin, 41 isolates showed resistance to Vancomycin,23 isolates showed resistance to Linezolid. The strains of Enterococci were multidrug resistant. Anitibiotic selection should be done judiciously based on the antibiotic sensitivity pattern of the organism.

Antibiotic Sensitivity Pattern of Bacterial Isolates from Respiratory Samples of Patients with Respiratory Tract Infection in a Tertiary Care Teaching Hospital of Pune: A Hospital Record-Based Study

Antibiotic Sensitivity Pattern of Bacterial Isolates from Respiratory Samples of Patients with Respiratory Tract Infection in a Tertiary Care Teaching Hospital of Pune: A Hospital Record-Based Study

Retrospective Study on the Antibiotic Sensitivity Pattern of Bacterial Isolates in Patients with Chronic Suppurative Otitis Media at the National Ear Care Centre Kaduna, Nigeria

Retrospective Study on the Antibiotic Sensitivity Pattern of Bacterial Isolates in Patients with Chronic Suppurative Otitis Media at the National Ear Care Centre Kaduna, Nigeria

Antibiotic Sensitivity Pattern Of Bacterial Isolates In Chronic Osteomyelitis In A Tertiary Care Teaching Hosptal Of North India.

Background: Chronic Osteomyelitis is a debilitated disease and is characterized by persistent and prolonged infection of the bone and its management remained an increasing challenge to clinicians due to growing resistances to antibiotics.
 Objective: The proposed study was conducted to ascertain the antibiotic sensitivity pattern of bacterial isolates in chronic osteomyelitis patients.
 Materials and methods: This descriptive study was conducted for a period of six months from June to November 2021. A total of 80 samples of pus and other exudates were collected from all the patients ≥ 18 years of age with either gender and diagnosed with chronic osteomyelitis˃6 weeks duration. The samples were sent for microbiological examination and culture. Various organisms were identified by standard methods. Antibiotic sensitivity pattern of detected microorganisms was also analysed. Patients with other form of arthritis, implant related osteomyelitis and who had antibiotic usage in last one week were excluded from our study.
 Results: Out of 80 patients, 73 (91%) showed growth of microorganisms whereas remaining 7 (9%) samples were sterile. The commonest bone affected in the study was tibia 53 (66.3%). Staphylococcus aureus was the most common bacteria isolated in 57 (78%) of patients. Maximum antibiotic sensitivity pattern was noted for vancomycin and clindamycin ( ˃90%) while maximum resistance was noted for penicillin in 22 (31%) of patients.
 Conclusion: Our study will thereby guide the clinicians in choosing appropriate antibiotics which not only contribute to better treatment but the judicious use of such antibiotics will also help in preventing emergence of resistance to drugs, which are still sensitive.

Microbial Contamination of Applied Instrument in Female Hair-Dressers Salon in Wukari, Taraba State, Nigeria

Hairdresser’s salons are public places that can contribute to the spread of viral, fungi and bacterial pathogens. However, little is known about the contamination of hairdressing tools by bacterial and fungal pathogens. Hence, this study was conducted to determine bacterial and fungal contaminants of tools used in hairdressing salons within Wukari metropolis, Taraba State. Eighty (80) different samples were collected from combs, brushes, rollers, and hairdryers used in hairdressing salons using sterile swab stick moistened with normal saline. Samples were cultured aerobically on nutrient agar, MacConkey agar, and sheep blood agar for bacterial isolation and potato dextrose agar for fungal isolation. Bacterial isolates were identified using conventional biochemical tests while fungal isolates were identified on the basis of their cell wall structure using the lactophenol cotton blue stain. Antibiotic sensitivity pattern of bacterial isolates was tested using the modified Kirby-Bauer disc diffusion method. Sixty-seven (83.75%) of the collected sample were positive for bacterial and/or fungal contamination, yielding twenty-two (22) and eighteen (18) isolates each of bacteria and fungi. The bacterial isolates were Staphylococcus aureus (81.82%), Staphylococcus epidermidis (13.64%), and Escherichia coli (4.64%) while the fungal isolates were Aspergillus fumigatus (31.25%), Aspergillus flavus (50%), Aspergillus niger (6.25%), Madurella grisea (6.25%), and Rhizopus stolonifera (6.25%). Bacterial isolates were generally sensitive to ciprofloxacin, gentamicin, rifampicin, ofloxacin, and streptomycin. The highest resistances were against cefuroxime, trimethoprim-sulfamethoxazole, ampicillin, and augmentin. The presence of these microorganisms on hairdressing tools is an indication of poor hygienic practices among hairstylists in Wukari and these tools can serve as vehicles for the transmission of bacterial pathogens. Hence, appropriate measures should be taken to reduce the microbial load from hairdressing salons instruments.

Antibiotic Sensitivity Pattern of Bacterial Isolates from Clinical Specimens of Patients Admitted in Intensive Care Unit

Introduction: Antibiotics resistance is increasing nowadays. Factor to antibiotic resistance is its misuse. There is no national guideline for use of antibiotics.
 Objective: Study was conducted to determine bacteriological profile, and the bacterial antibiotic sensitivity pattern of the pathogens isolated from patients in intensive care unit.
 Methodology: Study was conducted between 1st October 2020 and 30th March 2021. The samples collected from Intensive care units patients were processed in Microbiology Laboratory following Clinical and Laboratory Standard Institute guideline. Organisms were identified morphologically and biochemically and antibiotic sensitivity pattern was determined by disc diffusion methods.
 Result: Out of total 397 samples, only 46 were found to be culture positive, of which 32 (69.56%) were gram negative organisms and 14 (30.43%) were gram positive organisms. Out of gram negative organisms Klebsiella pecies 10 (21.73%) were predominant which is more sensitive to colistin, polymyxin B and tigecycline. Among gram positive organisms Staphycoccus aureus 12 (85.71%) was found more sensitive to Vancomycin than others.
 Conclusion: It is concluded that Klebsiella species and Staphylococcus aureus were predominant pathogens isolated from the patients. Regular surveillance of antibiotic sensitivity pattern and stewardship program will be useful in treating patients in Intensive care unit.

Prevalence and Antibiotic Sensitivity pattern of Bacterial Isolates from Catheterized UTI Patients: A Local Study

Prevalence and multidrug resistance among bacteria in catheter-associated urinary tract infections (CAUTIs) has been on the rise in recent times. Hence, the prevalence rate and antibacterial susceptibility of bacteria in CAUTIs in ICU patients was evaluated. A total of 120 patients admitted to the ICU of Nishtar Hospital, Multan, were recruited for this study. Both gram-positive and gram-negative bacterial isolates were characterized based on biochemical tests including catalase test, oxidase test, indole test, TSI test, citrate test, coagulase test and growth on 6.5% NaCl agar. The prevalence of bacterial species was Escherichia coli (32%), Staphylococcus aureus (26%), Pseudomonas spp. (18%), Proteus spp. (14%) and Enterococcus spp. (2%). A considerable degree of resistance against commonly prescribed antibiotics was observed. Gram negative bacteria showed resistance to ciprofloxacin, piperacillin-tazobactam and amikacin as well as susceptibility to imipenem, tigecycline and polymixin. Gram positive bacteria showed resistance to antibiotics such as piperacillin-tazobactam, ampicillin, gentamicin, oxacillin and ceftazidime suggesting the ineffectiveness of these antibiotics for treating bacterial infections among CAUTI patients and demonstrating the latest trends in antimicrobial drug resistance profile in local population.

METHICILLIN RESISTANCE STAPHYLOCOCCUS AUREUS (MRSA) FROM CSOM IN A TERTIARY CARE HOSPITAL

Introduction: Chronic Suppurative Otitis Media (CSOM) is one of the major cause of preventable hearing loss if treated promptly. Emergence of resistant strains in the world is of great concern. The aim of the present study was to determine the aetiology and antibiotic sensitivity pattern of bacterial isolates from CSOM cases with special emphasis on Methicillin Resistant Staphylococcus aureus. Materials and methods: Ear swabs were collected from 212 patients attending otorhinolaryngology department, GKMC, Chennai was processed in Microbiology lab. Direct gram staining was done and then inoculated into Blood, MacConkey and Nutrient agar. Bacterial identification was done using standard microbiological techniques. Antibiotic susceptibility was done by Kirby Bauer disc diffusion technique. Methicillin Resistant Staphylococcus aureus (MRSA) was detected by Cefoxitin disc diffusion method as per CLSI guidelines. Results: 60 Gram positive bacteria were isolated from collected ear swabs. Staphylococcus aureus 46(26.59%) was the most common bacteria among Gram positive organism followed by CONS 9(5.20%) and Enterococci 5(2.89%). Staphylococcus aureus showed 41(89.13%) was sensitive to amikacin and 40(86.95%) to ofloxacin. All the Gram Positive cocci were 100% sensitive to Vancomycin and Linezolid. 19.53% were confirmed as MRSA by cefoxitin disc diffusion method. Conclusion: Microbial pattern can vary in different location in different periods of time. Hence knowledge of appropriate aetiology and antimicrobial resistance pattern of CSOM helps in rational use of antibiotics and control of drug resistance.MRSA is emerging as an important pathogen in CSOM. Empirical antibiotics should be directed to gram positives, and especially, MRSA should be taken into consideration.

Urinary tract infections in Al-Kufa City Iraq and phenotypic detection of antimicrobial sensitivity pattern of bacterial isolates.

Urinary tract infections in Al-Kufa City Iraq and phenotypic detection of antimicrobial sensitivity pattern of bacterial isolates.

Evaluation of microbial flora of the external surface of housefly (Muscadomestica) in Umuahia Metropolis, Abia State, Southeast Nigeria

Objective:Houseflies are vectors responsible for the mechanical transmission of pathogens acquired from feeding in feces and decayed organic debris. Human consumption of such food without warming could lead to gastroenteritis, a major public health problem. The aim of this research was to evaluate the range of microbial pathogens associated with the external surfaces of fly vectors and to determine the antibiotic susceptibility pattern of the bacterial pathogens.Materials and Methods:A total of 150 houseflies were collected with a sterile net from different parts of Umuahia, Abia State. Their external surfaces were screened for bacteria, fungi, and protozoan parasites in the Microbiology Laboratory of Michael Okpara University of Agriculture, Umudike by standard microbiological procedures. Antibiotic sensitivity pattern of bacterial isolates was carried out by disc diffusion method.Results:The most frequently observed microorganisms wereEscherichiacoli(22.9%),Klebsiellaspp. (16.6%),Staphylococcusaureus(14.6%),Aspergillusspp. (28.3%), Mucor spp. (21.7%),Entamoebahistolytica(32.7%), andEndolimaxnana(30.9%). Houseflies from broken sewage had the highest total viable counts and frequency of bacteria, fungi, and parasites. Bacterial isolates from houseflies gotten from health-care facilities showed higher levels of multiple drug resistance to ampicillin and cotrimoxazole.Conclusion:In this study, pathogenic microorganisms were recovered from the external surface of houseflies, the vectoral agents of mechanical transfer of microbial contaminants to exposed food. Most of the microorganisms observed in this study are known pathogens that can cause gastroenteritis which is a public health concern.

Antibiotic Sensitivity Pattern of Bacterial Isolates in Neonatal Septicemia in Bangladesh

Antibiotic Sensitivity Pattern of Bacterial Isolates in Neonatal Septicemia in Bangladesh

Antibiotic sensitivity pattern of bacterial isolates from sputum samples of admitted patients with acute lower respiratory tract infections in a tertiary care teaching hospital of Tripura: a hospital record-based study

Background: Antibiotics are frequently used for various infectious diseases e.g., acute lower respiratory tract infection (ALRTI). But, injudicious use of antibiotics often leads to antibiotic resistance which is an emerging problem. The objective of this study was taken up to analyse the antimicrobial sensitivity pattern of pathogens isolated from the sputum samples of admitted patients suffering from ALRTI in a tertiary care teaching hospital.Methods: It is a hospital record-based study with a sample size of 393.Results: Klebsiella (52.16%) was the most common organism followed by Acinetobacter (13.49%) and Pseudomonas (13.23%) isolated from the sputum sample. Imipenem, piperacillin/tazobactam combination and gentamicin was sensitive against Klebsiella and Pseudomonas and the association were statistically significant. Acinetobacter was resistant to ceftriaxone.Conclusions: The commonest pathogens isolated from the sputum samples were Klebsiella followed by Acinetobacter and Pseudomonas. Imipenem, piperacillin/tazobactam combination and gentamicin was sensitive against Klebsiella and Pseudomonas.

Antibiotic Sensitivity Pattern of Bacterial Isolates among Diabetic Outpatients with Urinary Tract Infection in Pontianak

Diabetic patients are associated with a higher risk of infection. The research purposed to identify antibiotic susceptibility patterns among diabetic outpatients with urinary tract infection in Pontianak. An experimental study was performed for 13 bacterial isolates of diabetic outpatients with urinary tract infection in the Clinic of Diabetes Mellitus, Sultan Syarif Mohamad Alkadrie Hospital, Pontianak. The disc diffusion method was used to perform the susceptibility of antibiotics to the bacterial isolates. Among 13 isolates, the most common causative agent of urinary tract infection was Escherichia coli (53.85%), followed by Pseudomonas aeruginosa (30.77%). Klebsiella spp and Enterobacter aerogenes were 7.69%. Most isolates of bacteria of the study had a high sensitivity to Cefepime (92.31%), then followed by Levofloxacin, Amikacin, and Meropenem for 84.62%. The study revealed low sensitivity of bacteria to Amoxicillin/Clavulanate, Co-Trimoxazole, Cefazoline and Ceftriaxone (30.77%, 23.08 %, 23.08%, 23.08%, respectively). All bacterial isolates had high resistance to Ampicillin. Moreover, multidrug resistance observed among bacterial isolates.
 Keywords: antibiotic susceptibility, diabetes, urinary tract infections

Meropenem Sensitivity Pattern of Bacterial Isolates Causing Neonatal Sepsis in Special Care Neonatal Unit of Chattogram Medical College Hospital

Background: Neonatal sepsis is recognized as an important public health problem with a high morbidity and mortality, mostly occurring in developing countries. Treatment of neonatal sepsis has become a challenge with the emergence of carbapenemase producing bacteria. The study was done to evaluate the sensitivity pattern of bacterial isolates against meropenem causing neonatal sepsis. Materials and methods: A total of 100 clinically suspected neonatal sepsis cases was enrolled in the study. Bacteriological profile and meropenem sensitivity pattern was done. Results: Among the 100 suspected neonatal sepsis cases, 28% were culture positive and 72% were culture negative. Klebsiella species was the predominant isolated bacteria. Searching meropenem sensitivity pattern, 42.31% bacterial isolates were found to be sensitive and 57.69% were resistant to meropenem. Klebsiella spp. showed 40.00% sensitive to meropenem, whereas Acinetobacter spp. 25.00%, E. coli 66.67%, Staph. aureus 50.00% & Pseudomonas spp. 50.00% sensitive. Conclusion: Meropenem is the drug of choice for serious infections but bacterial resistance against it is increasing day by day.
 JCMCTA 2019 ; 30 (1) : 43-46

Epidemiology and antibiotic sensitivity pattern of asymptomatic bacteriuria during pregnancy: A cross-sectional study

Background: Asymptomatic bacteriuria (ASB) during pregnancy is associated with significant maternal and fetal complications. Continuous monitoring of antibiotic sensitivity pattern of bacterial isolates is required for appropriate management of ASB. Aims: The study aims to assess the prevalence, risk factors, and antibiotic sensitivity profile of ASB during pregnancy. Materials and Methods: A cross-sectional, hospital-based study was conducted in the pregnant women attending Obstetrics and Gynecology Department of a tertiary care hospital. A suitably designed data collection form was used to collect sociodemographic profile, obstetric history, and illnesses suffering from the participants. Urine specimens of the participants were processed for isolation and identification of bacterial species. Kirby–Bauer disc diffusion test was used to screen antibiotic sensitivity of the isolated bacteria. Statistical Analysis: Descriptive statistics were used to represent the sociodemographic data. Chi-square test was used to associate risk factors for the development of ASB during pregnancy. Results: A total of 1332 women were participated in the study. The prevalence of ASB among pregnant women was 86 (6.4%) and Escherichia coli (58; 67.4%) was the most predominant bacteria. Meropenem, cephalosporin's, and gentamycin were shown higher sensitivity in ASB. Advanced age, illiteracy, rural residency, multigravida, multiparity, history of abortion, and medical illnesses were strongly associated to develop ASB during pregnancy. Conclusion: E. coli were the most predominant isolated uropathogen in ASB. The study observed that there is a wide variation in the antibiotic sensitivity pattern of isolated organisms. Hence, there is a need of routine urine culture sensitivity screening in pregnant women for rational use of antibiotics and to reduce the antibiotic resistance.

Bacterial Contamination of Stethoscope in a Tertiary Care Hospital

Bacterial contamination of stethoscope and its role in transmission of nosocomial infection is little known among the healthcare workers of Bangladesh. The objectives of our study is to evaluate stethoscope handling and cleaning practices, to find out the bacterial agents contaminating the stethoscopes, to determine the antibiotic sensitivity patterns of bacterial isolates from stethoscopes, and to evaluate the relationship between stethoscope cleaning practices with contamination of stethoscope in Rajshahi Medical College Hospital. This observational cross sectional study was carried out in Rajshahi Medical College Hospital during March, 2017. One hundred doctors working in different departments of RMCH were chosen randomly and were asked to fill up a self-explanatory simple questionnaire. Samples from100 stethoscopes were obtained after swabbing the diaphragm and the bell of the stethoscope with a sterile swab moistened with saline. These swabs were immediately streaked onto blood agar, Mcconkey agar and chocholate agar following the standard protocol. Out of 100 stethoscopes examined for bacterial contamination, 19 stethoscopes (19%) were found to be contaminated. The organisms isolated were MRSA, pseudomonas aeruginosa, and E. coli. Among the 19 participants with bacterial contamination of stethoscope, 6 doctors (31.6%) said they used to clean their stethoscope at least occasionally and 13 doctors (68.4%) had never cleaned their stethoscope. This difference was found to be statistically significant (p value <0.005).TAJ 2017; 30(2): 1-6

Antibiotic Sensitivity Pattern of Bacterial Isolates in Patients with Chronic Rhinosinusitis in Kaduna, Nigeria.

Introduction A common practice in the management of patients with chronic rhinosinusitis (CRS), the empirical use of antibiotics may contribute to treatment failure and to the development of antimicrobial resistance. Objective To determine the antibiotic sensitivity pattern of aerobic and anaerobic bacteria associated with CRS. Methods This was a prospective cross-sectional study in which endoscopically guided middle meatal swabs (IBM Spss, version 16.0, Chicago, IL, USA) were aseptically taken from patients diagnosed with CRS after obtaining informed consent and ethical clearance. The samples were sent to the laboratory for qualitative and semiquantitative analysis via gram stain, aerobic, anaerobic cultures and antibacterial sensitivity tests. The collected data was analyzed using SPSS for Windows, version 16 (SPSS Inc, Chicago, IL, USA). Simple statistical parameters and paired sample t -test were used, as appropriate. Results There were 74 (56.92%) bacterial growths, out of which 55 (74.32%) were aerobic and 19 (25.68%) were anaerobic isolates, from a total of 130 patients. About 13 (17.5%–18%) of these bacterial growths yielded a mixed growth of aerobic and anaerobic isolates. The most common bacterial isolates were 26 (35.14%) Staphylococcus aureus , Haemophilus influenzae 9 (12.16%), Streptococcus viridians 8 (10.81%), and Streptococcus pneumoniae 5 (6.76%). Augmentin, ciprofloxacin, and Peflacine were found to be most effective, followed by levofloxacin, Rocephin, erythromycin and Zinat in that order. Conclusion Augmentin, ciprofloxacin and Peflacine have a sensitivity of 100%, while most of the organisms show resistance to Ampiclox, amoxicillin, and Septrin.

Prevalence and Antimicrobial Susceptibility of Bacterial Uropathogens Isolated from Pediatric Patients at Yekatit 12 Hospital Medical College, Addis Ababa, Ethiopia.

Background Urinary tract infection (UTI) is considered as the most common bacterial infection seen among the pediatric patients. Objective This study was carried out in order to determine the prevalence of urinary tract infection in pediatric patients, identify bacterial uropathogens responsible for the infection, and study the antibiotic sensitivity patterns of bacterial isolates. Materials and Methods A cross-sectional study designed and conducted from January to April 2014. Clean-voided midstream urine specimens were obtained from 384 pediatric patients less than or equal to 15 years in sterile universal bottles. Urine collected from each patient was inoculated onto CLED and blood agar plates using a calibrated inoculating loop with a capacity of 0.001 ml. Inoculated plates were incubated for 24–48 hours at 37°C at inverted position aerobically. Bacterial isolates were indentified and characterized by Gram stain and by using an array of standard routine biochemical tests. The antimicrobial susceptibility test was carried out by using the Kirby–Bauer disc diffusion method. Frequency distribution tables were used to describe the findings. Logistical regression was also used to estimate crude odds ratio (COR) with 95% confidence interval (CI) of positive responses to the different variables, and P values less than 0.05 were taken as statistically significant. Result In this study, a total of 384 patients (199 males and 185 females) aged less than or equal to 15 years from whom urine samples were collected were enrolled. Of these patients, 61 (15.9%) had significant bacteriuria. Of the 185 females, 36 (19.5%) came up with positive cultures, while 25 (12.6%) of the 199 males had significant bacteriuria, and the largest number of study subjects were below the age of 3 years, and the largest positive culture was obtained from this age group, accounting for 35 (57.4%.) out of 61 positive cultures. Bacterial species belonging to six genera were isolated and identified from 61 positive cultures, and the genera were Escherichia, Klebsiella, Staphylococcus, Proteus, Acinetobacter, and Enterococcus. E. coli was isolated in 28 cases (49.5 %), followed by Klebsiella spp. in 17 cases (27.9%), Staphylococcus spp. in 5 patients (8.2%.) (S. aureus in one and coagulase-negative staphylococci in 4 cases), Enterococcus in 7 cases (11.5%), Proteus spp. in 3 cases (4.9%), and Acinetobacter in one case (1.6%). Of the bacterial isolates, E. coli was found out to be the most common pathogen followed by Klebsiella spp. Furthermore, E. coli and Klebsiella spp. were the most common pathogens in female patients accounting for 71.4% and 64.7%, respectively. Regarding susceptibility tests, E. coli and Klebsiella spp. were not 100% susceptible to any of the 11 antibiotics tested. Acinetobacter spp. had 100% resistance to three antibiotics: gentamicin (GN), trimethoprim-sulfamethoxazole (SXM), and augmentin (AMP). But they were 100% susceptible to ciprofloxacin (CIP), cefuroxime (CXM), norfloxacin (NOR), and ceftazidime (CAZ). On the contrary, Proteus spp. was 100% sensitive to all drugs except to nitrofurantoin. Species of Enterococcus had resistance of 71.4% to chloramphenicol (C) and 85.7% to both SXM and erythromycin. S. aureus was 100% susceptible to almost all drugs, while coagulase-negative staphylococci were not as susceptible as S. aureus. Multidrug resistance to two or more drugs was observed in 73.7% of the bacterial isolates. Conclusion This study determined the prevalence of urinary tract infection in pediatric patients and highlighted the major bacterial uropathogens involved in UTI for the first time in the country. Furthermore, bacterial pathogen species and their frequency was consistent with the usually reported pattern, with E. coli being the most common organism isolated in cases of urinary tract infections followed by Klebsiella spp. Most of the bacterial isolates were multidrug resistant, and it is therefore suggested that appropriate antimicrobials should be administered to reduce the risk of multidrug resistant organisms developing and avert ineffectiveness of antibiotics. This condition indicates that antibiotic selection should be based on knowledge of the local prevalence of bacterial organisms and antibiotic sensitivities rather than empirical treatment. The present study indicated that ciprofloxacin (CIP), ceftazidime (CAZ), cefotaxime (CTX), cefuroxime (CXM), clindamycin (DA), and ceftriaxone (CRO) were the best antibiotics for the treatment of Gram-negative and Gram-positive bacterial uropathogens, respectively, in the study area relatively.