Culture Seedlings Research Articles

Research on the rapid tissue breeding technology of the endangered plant Cibotium barometz (L.) J. Sm.

Cibotium barometz (L.) J. Sm., a fern, holds significant value in traditional Chinese medicine. This research aimed to establish a rapid tissue breeding technology for Cibotium barometz (L.) and provide a theoretical basis for its artificial breeding and industrial planting. The optimum conditions for spores germination were selected, including both the best sterilization time and the ideal spore germination medium. Subsequently, the optimal medium for proliferation and differentiation were chosen, and the optimal concentration of plant hormones was screened to determine the optimal medium. The rooting experiment was performed in two single-factor experiments by using differentiated sporophytes as materials. These experiments showed that the optimal sterilization time was 11 min and the best medium for germination was 1/8MS + 0.02 mg/L IAA. MS medium was suitable for proliferation, while WPM medium was suitable for sporophyte differentiation. The best medium for growth was MS + 1.0 mg/L IAA + 0.5 mg/L 6-BA. In the absence of sucrose, the seedlings barely grew and failed to differentiate into sporophytes. They showed optimal growth and differentiation at a sucrose concentration of 20 g/L. The plant growth regulators plant growth regulators used, including IAA, NAA, 6-BA, and GA3, showed more effective differentiation, wherease KT had a slightly weaker effect. The rooting rate reached the maximum when the concentrations of ABT and IBA were at 1.5 mg•L− 1 and IAA was at 1.0 mg•L− 1. The IBA had a significant effect on the average and the longest root length, while the three PGRs had no obvious effect on the average or maximum diameter. Plant tissue culture seedlings still have good genetic stability after subsequent generation. In this study, the optimal cultivation conditions for rapid breeding technology in tissue culture, covering stages from germination and growth to differentiation and rooting, were obtained. Moreover, sporophytes were successfully transplanted to the external environment. This study provided a theoretical reference for the industrial planting of C. barometz.

Оздоровление растений винограда сорта Красностоп АЗОС от вируса скручивания листьев -1, -2 и -3 (grapevine leafroll-associated viruses -1, -2 and -3) методом термотерапии

Abstract. The grapevine leafroll-associated virus is one of the most harmful and economically significant. It is known from scientific literature sources that in the vineyards of the Krasnodar region this virus is spread over an area of 50 %. The problem of increasing the phytosanitary stability of grape plantations necessitates the constant improvement of protection systems, in which methods of improving planting material are of great importance. To date, there are several methods for inactivating viral particles and freeing individual plant organs from viral infection – in vitro, thermo- and chemotherapy. Thermotherapy is one of the widely used methods that involves growing and disinfecting vines at temperatures that can delete or inactivate viruses. This method focuses on inactivating viruses rather than completely removing them. Thermotherapy has been tested in the process of obtaining initial healthy grape plants from the grapevine leafroll-associated virus GLRaV-1, GLRaV-2 и GLRaV-3. The objectives of the research are the selection of thermotherapy regimens for the release of grape cuttings from the grapevine leafroll-associated virus (GLRaV-1, GLRaV2 и GLRaV-3). As a result of the research, it was found that the use of thermotherapy of 35 ℃ and 40 ℃ for 20, 30, 40, 50 minutes did not affect the elimination of the grapevine leafroll-associated virus, the yield of diseased plants after treatment was 100 %. A positive result in the healing of plants from the grapevine leafroll-associated virus is achieved at a temperature of 45 ℃ and 50 ℃ (exposure time is 20, 30, 40, 50 minutes). The number of virus-free plants ranges from 45.4 % to 59.8 %. The inclusion of thermotherapy in the process of phytosanitary breeding, as well as in the cultivation of seedlings for mother grape plantations, will significantly increase the guarantee of laying industrial grape plantations with planting material free from phytoplasmic, viral, bacterial and fungal infections. Keywords: grapes, cuttings, grapevine leafroll-associated virus GLRaV-1, GLRaV-2 and GLRaV-3, thermotherapy, thermal stability, elimination, healthy planting material Financing source: The research was carried out within the framework of the Federal project "Development of large-scale scientific and scientific-technological projects in priority research areas" of the national project "Science and Universities"

Growth stimulant influence on biometric indicators of oak seedlings in the Bukovyna Sub-Carpathian region

The use of growth stimulants for the cultivation of oak seedlings in the Bukovyna Sub-Carpathian region allows to accelerate their development and increase their sustainability in an environment where there is no natural forest regeneration. The aim of the study was to evaluate the effect of growth stimulants at different multiplicity of treatments during the growing season on the biometric parameters of Quercus robur L. Seedlings were grown in Ecotherm containers in the glasshouse of the base nursery. The composition of the substrate for filling the containers is a mix of peat, sand and black soil in equal proportions with mycorrhiza from under the forest canopy of oak stands. The results of the effect of growth stimulants on the biometric parameters of one-year-old seedlings of common oak with a closed root system are presented. Plant growth stimulants were used for spraying and irrigation seedlings during their cultivation. The results of the research indicate a positive effect of the use of growth stimulants on the seedlings of common oak. All experimental variants showed a positive effect on the biometric parameters of one-year-old oak seedlings at three, six and nine times of feeding with growth stimulants during the growing season. The difference in the variants with the use of plant growth stimulants compared to the control in height is up to 27%, in the length of the root system – up to 43%, and in the total length – up to 29%, accordingly, depending on the dose of the stimulants and the frequency of treatment during the growing season. Refined data on the biometric parameters of one-year-old seedlings of common oak with a closed root system grown in closed ground conditions were obtained. The data obtained are useful for forest restoration in regions with damaged or degraded ecosystems where natural regeneration is limited

A novel nitrogenous coumarin: The promising antiviral therapy against white spot syndrome virus in the culture of shrimp seedlings

White spot disease (WSD), attributed to the white spot syndrome virus (WSSV), represents a substantial danger to the international shrimp sector, resulting in considerable losses in both production and economic terms. Nevertheless, there are currently no approved therapeutic measures to control the outbreak. This study involved the synthesis of 15 new compounds for the assessment of their anti-WSSV efficacy in Litopenaeus vannamei post-larvae. Among these compounds, N-(4-methyl-2-oxo-2H-chromen-7-yl)acetamide (P13) exhibited a maximum antiviral response of > 90 %, with a median effective concentration (EC50) < 12 mg/L, making P13 the most promising antiviral compound. In the cases where the larvae were simultaneously incubated with WSSV and P13, as well as where the larvae were first infected with WSSV and then treated with P13, the replication of WSSV was significantly inhibited. However, pre-treatment with P13 had minimal impact on WSSV multiplication, suggesting a potential therapeutic effect. Simultaneously, the cumulative mortality rate of WSSV-infected post-larvae decreased in the presence of P13. After the shrimp larvae were infected with WSSV, continuously changing the aquacultural water containing DMSO resulted in a mortality rate of 80 % at 120 hours post-infection (hpi); whereas continuously changing the aquaculture water containing P13 resulted in a mortality rate of 45 %. Given that P13 remains stable in farmed water for up to two days, it is recommended to regularly replace the medication to eliminate the virus threat. In conclusion, P13 demonstrates a positive anti-WSSV activity and holds potential for further development as an antiviral therapy against WSSV infection.

Development of artificial lighting system for light supplementation in smart greenhouses with agrivoltaic systems

This work developed two smart greenhouses for the protected cultivation of banana and orchid seedlings. The greenhouses feature control and automation systems, bioclimatic architecture and strategies, and energy systems. A sensor network monitors indoor environmental variables, and a weather station monitors outdoor environmental variables. A computational system based on artificial intelligence is responsible for the autonomous control of the greenhouses. This article presents the development of an artificial lighting system for light supplementation in smart greenhouses. The artificial lighting system was defined from modeling and simulation in a computational tool. The proposed system was installed in two smart greenhouses in southern Brazil. The article also presents the development of methods for estimating electricity consumption based on assumptions and operational profiles of the system and the experimental measurement of the artificial lighting system performed according to the procedure described in technical standards. The article presents the energy performance of the agrivoltaic system applied to smart greenhouses. From August 2022 to July 2023, the final yield of the agrivoltaic systems of the smart greenhouses was 1418.91 kW h/kWP and 1490.68 kW h/kWP. The annual electricity consumption of the artificial lighting systems of the smart greenhouses ranges between 3290 kW h and 3825 kW h.

Maize insensitivity to culture filtrates from Exserohilum turcicum is related to quantitative resistance

AbstractTurcicum leaf blight (TLB) of maize is caused by Exserohilum turcicum. The TLB resistance is mainly associated with qualitative race‐specific resistance that is linked to several Ht genes namely Ht1, Ht2, Ht3, HtM, HtN, HtNB and HtP. However, quantitative TLB resistance also occurs, but its mechanisms are poorly understood. In this study, tolerance or insensitivity to chlorosis and necrosis of E. turcicum culture filtrate was associated with quantitative TLB resistance. A novel detached maize seedling assay was developed for E. turcicum culture filtrate using methanol‐treated modified Fries medium. Screening of E. turcicum isolates of races 2, 3, 23, 3 N, 23 N and 123 N against 61 maize inbred lines with diverse levels of resistance to TLB was conducted in the greenhouse by fungal inoculation and the detached seedling culture filtrate assay. Using an isolate of race 123 N, which can overcome all the qualitative resistance genes, a simple linear regression model (R2 = 0.68, p = .90) for the 61 inbred lines was established between disease severity from foliar inoculations and culture filtrate symptom rating of the detached seedling assay. Two maize lines CML474 and CML483 had relatively high culture filtrate symptom ratings but very low disease severity with foliar inoculation indicating that these sources of quantitative TLB resistance may be unrelated to tolerance to E. turcicum culture filtrates, whose effect on seedlings could be due to the toxin known as HT‐toxin or monocerin.

Morpho-physiochemical traits and metabolic analysis reveal the rapeseed responses under large clod sized soil conditions

Soil clod size is an important factor affecting seedling growth; besides, cultivation of strong seedlings is important to improve yield and benefit of direct-seeding rapeseed. To simulate the open field conditions, two rapeseed cultivars were grown in different clod sized soil to study morpho-physiochemical responses at five leaf stage, beside applying metabolite profiling. Moreover, foliar application via alpha-linolenic acid (ALA) was applied to improve the adaptability of rapeseed to large clod sized soil. Our results showed that soil water content was decreased in large clod sized soil (K2) versus small clod sized soil (K1), which decreased seedling weight, shoot length and root vitality, while increasing root length. Furthermore, osmolyte contents and peroxidase (POD) and catalase (CAT) activity were decreased with increasing malondialdehyde content in studied rapeseed cultivars under K2 conditions. Moreover, phospholipase A2 (PLA2) of phospholipid metabolism and its related enzymes were decreased, while its content and gene expression were increased. Besides, ALA metabolism was up-regulated, while lipoxygenase (LOX) activity, content and its gene expression were elevated under K2 conditions. Metabolic profiling showed that lipid metabolism was significantly changed than other identified components, which is synthetic precursors of various secondary metabolites. Compared with K1, the metabolic pathways of ALA and pyrimidine were up-regulated, while the metabolic pathways of glycerophospholipids and purines were down-regulated in both studied cultivars under K2 conditions. Moreover, ALA application enhanced seedling growth by improving root length, shoot and root dry weight, root vitality, ALA and proline contents, while reducing the malondialdehyde (MDA) content of two rapeseed cultivars under K2 conditions. Taken together, our study provided a theoretical basis and technical support for the soil tillage and cultivation of direct-seeding rapeseed.

Combined effects of cadmium and simulated acid rain on soil microbial communities in the early cultivation of Populus beijingensis seedlings

The combined cadmium (Cd) and acid rain pollution poses a significant threat to the global ecological environment. Previous studies on the combined adverse effects have predominantly focused on the aboveground plant physiological responses, with limited reports on the microbial response in the rhizosphere soil. This study employed Populus beijingensis seedlings and potting experiments to simulate the impacts of combined mild acid rain (pH=4.5, MA) or highly strong acid rain (pH=3.0, HA), and soil Cd pollution on the composition and diversity of microbial communities, as well as the physiochemical properties in the rhizosphere soil. The results showed that Cd decreased the content of inorganic nitrogen, resulting in an overall decrease of 49.10 % and 46.67 % in ammonium nitrogen and nitrate nitrogen, respectively. Conversely, acid rain was found to elevate the content of total potassium and soil organic carbon by 4.68 %-6.18 % and 8.64–19.16 %, respectively. Additionally, simulated acid rain was observed to decrease the pH level by 0.29–0.35, while Cd increased the pH level by 0.11. Moreover, Cd alone reduced the rhizosphere bacterial diversity, however, when combined with acid rain, regardless of its intensity, Cd was observed to increase the diversity. Fungal diversity was not influenced by the acid rain, but Cd increased fungal diversity to some extend under HA as observed in bacterial diversity. In addition, composition of the rhizosphere bacterial community was primarily influenced by the inorganic nitrogen components, while the fungal community was driven mainly by soil pH. Furthermore, “Metabolism” was emerged as the most significant bacterial function, which was markedly affected by the combined pollution, while Cd pollution led to a shift from symbiotroph to other trophic types for fungi. These findings suggest that simulated acid rain has a mitigating effect on the diversity of rhizosphere bacteria affected by Cd pollution, and also alters the trophic type of these microorganisms. This can be attributed to the acid rain-induced direct acidic environment, as well as the indirect changes in the availability or sources of carbon, nitrogen, or potassium.

Transcriptome analysis unravels differential genes involved in essential oil content in callus and tissue culture seedlings of Lavandula angustifolia

Lavender (Lavandula angustifolia Mill.) essential oil is of high medicinal and economic importance. The preliminary results of this study found that lavender tissue culture seedlings contained 2.1% essential oil, which was 21 times that of callus tissue. However, the mechanisms involved in its synthesis are unclear. This study used transcriptome sequencing of L. angustifolia callus and tissue culture seedlings to compare changes in differentially expressed genes (DEGs) and metabolic pathways. Transcriptome analysis revealed 2394 DEGs in callus and tissue culture seedlings. Several DEGs were enriched in plant hormone signal transduction, as well as the biosynthesis of terpenoids and other secondary metabolites. In the terpenoid biosynthesis pathway, 93 genes were differentially expressed, and four receptors protein genes (BRI1) of brassinosteroid (BR) hormone were significantly differentially expressed. This study provides deeper insights into regulatory genes involved in terpenoid biosynthesis and BR signal transduction in L. angustifolia, contributing to the genetic improvement of L. angustifolia.

Effects of Humic Acid-Copper Interactions on Growth, Nutrient Absorption, and Photosynthetic Performance of Citrus sinensis Seedlings in Sand Culture

Effects of Humic Acid-Copper Interactions on Growth, Nutrient Absorption, and Photosynthetic Performance of Citrus sinensis Seedlings in Sand Culture

Influence of Entrepreneurial Activities on Adoption of Tissue Culture Banana Seedlings in Tanzania.

Banana is the world’s most popular fruit, with an estimated global export value of 7 billion dollars per year; they are also one of the most domestic foods and are traded by Tanzania farmers. However, approximately 15% of the global banana production is involved in trade. The inadequate adoption of Tissue Banana Seedlings has led to the assessment of the Influence of Entrepreneurial Activities on the Adoption of Tissue Culture Banana Seedlings in Tanzania-Kilimanjaro particularly. The study adopted a positivism philosophy, a cross-sectional survey design, and purposive sampling techniques targeting 350 farmers. Questionnaires were used for data collection, Binary Logistic Regression was applied for data analysis, the results show Entrepreneurial significantly influenced the adoption of Tissue culture seedlings, and Tissue culture seedlings offer more business opportunities than local suckers. Therefore, the study recommends promoting Entrepreneurial activities toward the adoption of tissue culture banana seedlings in Tanzania and developing countries

Chili Growth-Yield Improvement under Different Experience-Creativity Farmer Levels, Agronomical Components, and Their Partial Economic Analysis

Chili (Capsicum annuum L.), though it is one of the strategic and important vegetable commodities in Indonesia; still faces a main problem, i.e. low productivity with 7.78 tons ha-1 nationally and 6.45 tons ha-1 specifically in Jawa Tengah. Consequently, the improvement of chili growth yield under different experience and creativity farmer levels (ECFLs), components of agronomy (ACs), and their economic analysis partially was addressed as the main objective of the research. C. annuum ‘Akar’, healthy and farmer seedlings were used in the field research. The factorial experiment was arranged in a randomized complete block design with three replications. ECFLs from high (ECFL-1), moderate (ECFL-2), less (ECFL-3), and initially developed (ECFL-4) were used as the first treatment, and five different ACs with gradual component reduction of ACs-1, ACs-2, ACs-3, ACs-4 and ACs-5 as second treatment. The research clearly revealed that different seedlings, ECFLs, and ACs significantly affected the ‘Akar’ growth yield. Cultivation of the healthy seedlings by ECFL-1, who had ± thirty years’ experience in chili cultivation and fast response to all and potential problems during chili cultivation, under ACs-1, as a complete and optimal ACs, increased vegetative growth and yielded 417.8 flowers and 289.7 fruits plant-1, 1,040.6 g chili plant-1, and 1,044.8 kg chili plot-1; 110-337% improvement; IDR. 16,750,081 farmer income from 250 m2 plot size; and 4.77 R/C ratio, respectively. However, for positive income, R/C ratio, and low production cost for all farmers, further applications of ACs-2 were promisingly chosen. Entirely the optimal growth yield of chili was established by using healthy seedlings, choosing suitable Acs, and paying more attention to the ECFL. The results can be applied to other chili types and varieties.

Development of indirect somatic embryogenesis and plant regeneration system with immature embryos of the cultivated traditional Chinese medicinal herb Polygonatum cyrtonema

The dried rhizome of Polygonatum cyrtonema represents a highly valued traditional Chinese medicine. With a growing demand, the imperative arises for the establishment of an efficient tissue culture system for large-scale propagation. Here, we successfully developed a tissue culture system capable of yielding P. cyrtonema seedlings through somatic embryogenesis using immature embryos as explants within a 120-day timeframe. An optimum callus induction rate was achieved through a combination of 1.0 mg/L 6-BA and 0.5 mg/L 2,4-D in MS medium, resulting in the formation of numerous highly active callus. Subsequent transfer of primary callus to a medium containing 1.0 mg/L 6-BA and 1.0 mg/L 2,4-D in MS medium for 30 days yielded induction rates of 97.78%, 89.62%, 73.33%, and 66.67% for globular, heart, torpedo, and cotyledonary embryos, respectively. The highest rate of somatic embryo normal germination (73.33%) was observed in the MS+6-BA 2.0 mg/L+NAA 1.0 mg/L medium. Subsequent transfer of germinated somatic embryos to MS+IAA 0.5 mg/L+IBA 1.0 mg/L medium for 20 days resulted in robust tissue culture seedlings, with a survival rate of 93.33% when transplanted into a seedling substrate consisting of soil: perlite (3:1, v/v). This study not only marks the inaugural instance of somatic embryogenesis in Polygonatum species but also paves a foundation for the advent of breeding traditional Chinese medicinal herb P. cyrtonema via recent advanced biotechnological strategies.

The Influence of Observability on The Adoption of Tissue Culture Banana Seedlings in Tanzania

In seedling technology, Banana seedlings are undergoing a profound transformation with the integration of advanced innovation, referred to as tissue culture banana seedling. The study delves into the science of seedling technology and innovation adoption within the banana farming sector. We explore the dynamic interactions between tissue culture innovation and farmer adoption. The study adopted a positivism philosophy, cross-sectional survey design, and purposive sampling techniques. A sample size of 350 banana farmers in Kilimanjaro was drawn from the population of the study using the Yamen formula for sample size determination. Since the outcome variable was dichotomous the binary logistic regression model was employed. The result indicates that the highest mean was for the statement banana tissue culture seedling grow faster compared to local sucker (M=3.75), indicating the speed of growth is seen as a clear observable benefit. Standard deviations were relatively low, ranging from 0.826 to 0.872, indicating less variation in responses. These results suggest smallholder farmers perceive tissue culture banana seedlings as having moderately high observability, especially related to the visible benefits of faster planting growth and income generation compared to using local banana seedlings. We shed light on the adoption of tissue culture banana seedlings. Adoption is reshaping traditional seedling suckers practices, enhancing productivity and business opportunity. Farmers' adoption will lead to economic development and job creation. Farmers markets provide opportunities for small farmers and businesses to sell their products, and support to meet the growing demand for locally produced food.

The Impact of Light Intensities on the Phenotypic Parameters of Cucumber Seedlings at Three Developmental Stages

Industrialized seeding production is one of the most important methods used to raise seedlings in a standardized and efficient manner. However, its consumption of light energy limits its development. In this paper, we studied the effects of LED light intensities (30 (L1), 60 (L2), 90 (L3), 120 (L4), 150 (L5), 180 (L6), and 210 (L7) µmol·m−2·s−1) on the phenotypic parameters and health index of seedlings during different growth stages (cotyledon flattening (CF), one bud and one leaf (OBOL), and one bud and two leaves (OBTL)). Ten out of sixteen plants were selected to measure the parameters in every treatment. The control group was treated with 150 µmol·m−2·s−1 all the time. The results show that the height of the L1-treated plants was greatest during the cotyledon flattening stage. The L7 treatment produced the largest stem diameter, the greatest biomass, the largest leaf area, and the largest leaf circumference, the values of which were 2.61 mm, 3.3 g, 60.67 cm2, and 39.71 cm, respectively. During the one bud and one leaf stage, the L1 treatment produced the tallest plants. The L7 treatment resulted in the largest stem diameter, the greatest biomass, the largest leaf area, and the largest leaf circumference. During the one bud and two leaves stage, the plant height, stem diameter, leaf circumference, and biomass produced of the L6 treatment were the greatest, with values of 68.37 mm, 3.09 mm, 51.54 cm, and 4.47 g. L5 resulted in the largest leaf area (63.91 cm2). The seedling index of the three stages showed an inflection point at the L4 level. The experimental results showed that the light intensity required by the cucumber seedlings was 120 µmol·m−2·s−1, 150 µmol·m−2·s−1, and 120 µmol·m−2·s−1 during the three stages, from the cotyledon flattening stage to the one bud and two leaves stage. The results provide references regarding the light required during the cultivation of cucumber seedlings.

Growth, Physiological, and Transcriptome Analyses Reveal Mongolian Oak Seedling Responses to Shading

Mongolian oak (Quercus mongolica) is a common building material and landscaping tree species in northern China, with significant economic and ecological value. Its seedling growth is inhibited by high light intensity, but the mechanism by which light stress affects the growth and development of its seedlings remains unclear. In this study, we investigated the phenotypes, physiological processes, and molecular responses of 3-year-old Mongolian oak seedlings under different light treatments: full light (Sck), light shading (S1; 40% light), moderate shading (S2; 20% light), and severe shading (S3; 3% light). Compared to Sck, the S1 and S2 treatments resulted in higher leaf area, photosynthetic pigment content, photosynthesis rates, soluble sugar contents, and soluble protein contents in Mongolian oak seedlings. The S1 and S2 treatments also promoted seedling height and diameter growth and resulted in lower degrees of membrane lipid peroxidation, cell membrane permeability, and antioxidant enzyme activity. In contrast, severe shading (S3) significantly inhibited seedling height and diameter growth due to the lower net photosynthetic rate, and exhibiting higher degrees of membrane lipid peroxidation and cell membrane permeability. Shading treatments (S1 and S2) alleviated the negative effects of strong light on the growth and development of Mongolian oak seedlings, with the S2 treatment having the greatest effect. However, severe shading (S3) inhibited growth and development. A total of 3726 differentially expressed genes (DEGs) were detected in leaves under different shading treatments in RNA sequencing analysis. Among these, 1691, 3150, and 824 DEGs were detected in the Sck-S1, Sck-S2, and S1-S2 comparison groups, respectively. The different shading treatments determined common expression regulation pathways, including carotenoid biosynthesis, photosynthetic antenna proteins, and mitogen activated protein kinase (MAPK) signal transduction. Shading induced increases in gene expression levels in light harvesting complexes, which are related to changes in gene expression in the photosynthetic system, leading to changes in photosynthetic physiology. The expression levels of genes related to reactive oxygen species signal perception and activation enzymes were upregulated in Sck. Together, these findings revealed the response mechanisms of Mongolian oak seedlings to different shading levels at the physiological and molecular levels, providing a scientific basis and technical support for the cultivation and large-scale production of Mongolian oak seedlings.

Border cell population size and oxidative stress in the root apex of Triticum aestivum seedlings exposed to fungicides.

Fungicides reduce the risk of mycopathologies and reduce the content of mycotoxins in commercial grain. The effect of fungicides on the structural and functional status of the root system of grain crops has not been studied enough. In this regard, we studied the phytocytotoxic effects tebuconazole (TEB) and epoxiconazole (EPO) and azoxystrobin (AZO) in the roots of Triticum aestivum seedlings in hydroponic culture. In the presence of EPO and AZO (but not TEB) inhibition of the root growth was accompanied by a dose-dependent increase in the content of malondialdehyde, carbonylated proteins, and proline in roots. TEB was characterized by a dose-dependent decrease in the total amount of border cells (BCs) and the protein content in root extracellular trap (RET). For EPO and AZO, the dose curves of changes in the total number of BCs were bell-shaped. AZO did not affect the protein content in RET. The protein content in RET significantly decreased by 3 times for an EPO concentration of 1µg/mL. The obtained results reveal that the BC-RET system is one of the functional targets of fungicides in the root system of wheat seedlings. Studied fungicides induce oxidative stress and structural and functional alterations in the BC-RET system that can affect their toxicity to the root system of crops.

Effects of Different Biostimulants on Growth and Development of Grapevine Seedlings under High-Temperature Stress

High temperatures significantly affect the growth and development of grapevines, cause irreversible damage to plants, and severely impact grape production and quality. Biostimulants can promote the growth of plants and enhance their resistance to adverse stress. However, the effects of biostimulants on grapevines under high temperatures have not been studied in detail. To analyze the effects of various biostimulants on the growth and development of grape seedlings under high temperatures, we measured chlorophyll fluorescence parameters with observed seedling phenotypes under high temperatures in open field conditions in Turpan. We conducted a comprehensive analysis of the effects of different biostimulants on the growth, development, and photosynthesis of grapevine seedlings. Our study aimed to provide scientific evidence to improve cultivation methods for grapevines under high-temperature stress. The results revealed that biostimulants have a positive effect on promoting the growth of grapevine seedlings under high-temperature stress conditions. They also positively affect the accumulation of chlorophyll components in grapevine leaves, inhibiting chlorophyll degradation and maintaining photosynthesis. However, the effects of different biostimulants were inconsistent. A comprehensive analysis revealed the following effectiveness order: T2 &gt; T1 &gt; T3 &gt; Control. These findings suggest that T2 is the most effective in alleviating high-temperature stress and promoting grapevine growth. We recommend the use of T2 to improve the cultivation of grapevine seedlings during high-temperature periods. This has implications for grape production in hot and arid climatic areas.

VARIABILITY OF ANATOMICAL AND MORPHOLOGICAL TRAITS OF PINUS NIGRA AND PINUS SYLVESTRIS SEEDLINGS AFFECTED BY DIFFERENT CONTAINER TYPE

In the paper was analysed the influence of three different container types, used for cultivation of Pinus nigra and Pinus sylvestris seedlings, on dimensions of their anatomical (resin ducts width, resin ducts number, tracheid number, tracheid width, wood rays height) and morphological (height, root collar diameter, sturdiness coefficient) elements, and on proportion of wood, bark and pith, as well. Two-factorial ANOVA showed that container type affects a lot all investigated anatomical traits by both species, but on the other side, these species varied between each other just in terms of tracheid width and wood rays height. Based on descriptive statistics, significantly lower values of all studied anatomical elements were recorded by biodegradable compared to plastic containers. As for P. nigra seedlings, they showed the best anatomical performance in Plantagrah I, while Hiko V-120 SS was the most suitable for P. sylvestris. The highest proportion of pith and bark was recorded in biodegradable container. As for morphological parameters, such as height and root collar diameter, higher values were recorded by plastic containers.

The Influence of Observability on The Adoption of Tissue Culture Banana Seedlings in Tanzania

In the seedling’s technology, Banana farming is undergoing a profound transformation with the integration of advanced technologies, referred to as tissue culture banana seedlings. The study delves into the empires of seedling technology and innovations adoption within the banana farming sector. We explore the dynamic interactions between Tissue culture innovation, and the farmer's adoption. The study adopted positivism philosophy, a cross-sectional survey design, and purposive sampling techniques. A sample size of 350 banana farmers in Kilimanjaro was drawn from the population of the study using Yamen formula for sample size determination. Since the outcome variable was dichotomous the binary logistic regression model was employed. The results indicate that, the highest mean was for the statement “Banana tissue culture seedlings grow faster compared to local suckers” (M=3.75), indicating the speed of growth is seen as a clear observable benefit. Standard deviations were relatively low, ranging from 0.826 to 0.872, indicating less variation in responses. These results suggest smallholder farmers perceive tissue culture banana seedlings as having moderately high observability, especially related to the visible benefits of faster planting growth and income generation compared to using local banana seedlings. We shed light on the adoption of tissue culture seedlings. Adoption is reshaping traditional suckers’ practices, enhancing productivity and business opportunity. Farmers adoption will leads to the economic development and job creation. Farmers markets provide opportunities for small farmers and businesses to sell their products, and support to meet the growing demand for locally produced food.