Abstract
Abstract. The grapevine leafroll-associated virus is one of the most harmful and economically significant. It is known from scientific literature sources that in the vineyards of the Krasnodar region this virus is spread over an area of 50 %. The problem of increasing the phytosanitary stability of grape plantations necessitates the constant improvement of protection systems, in which methods of improving planting material are of great importance. To date, there are several methods for inactivating viral particles and freeing individual plant organs from viral infection – in vitro, thermo- and chemotherapy. Thermotherapy is one of the widely used methods that involves growing and disinfecting vines at temperatures that can delete or inactivate viruses. This method focuses on inactivating viruses rather than completely removing them. Thermotherapy has been tested in the process of obtaining initial healthy grape plants from the grapevine leafroll-associated virus GLRaV-1, GLRaV-2 и GLRaV-3. The objectives of the research are the selection of thermotherapy regimens for the release of grape cuttings from the grapevine leafroll-associated virus (GLRaV-1, GLRaV2 и GLRaV-3). As a result of the research, it was found that the use of thermotherapy of 35 ℃ and 40 ℃ for 20, 30, 40, 50 minutes did not affect the elimination of the grapevine leafroll-associated virus, the yield of diseased plants after treatment was 100 %. A positive result in the healing of plants from the grapevine leafroll-associated virus is achieved at a temperature of 45 ℃ and 50 ℃ (exposure time is 20, 30, 40, 50 minutes). The number of virus-free plants ranges from 45.4 % to 59.8 %. The inclusion of thermotherapy in the process of phytosanitary breeding, as well as in the cultivation of seedlings for mother grape plantations, will significantly increase the guarantee of laying industrial grape plantations with planting material free from phytoplasmic, viral, bacterial and fungal infections. Keywords: grapes, cuttings, grapevine leafroll-associated virus GLRaV-1, GLRaV-2 and GLRaV-3, thermotherapy, thermal stability, elimination, healthy planting material Financing source: The research was carried out within the framework of the Federal project "Development of large-scale scientific and scientific-technological projects in priority research areas" of the national project "Science and Universities"
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