Seed Kernel Oil Research Articles

Evaluation of Spent Engine Oil and other Natural Materials on the Emergence of Harvester Ants (Messor galla Forel) in North Eastern Nigeria

Harvester ants (Messor galla Forel) defied various control strategies. Two field experiments were conducted to evaluate the effects of Spent Engine-Oil and other nature-based materials on their emergence in Maiduguri, Borno State of Nigeria. The treatments evaluated are spot application of spent engine-oil and some natural materials in experiment 1 and individual and equal mixture of Pure Neem Seed Kernel oil and Spent Engine-oil in experiment 2. Spot application of permethrin served as control. Experiment 1 results shows no significant difference (p > 0.05) between permethrin and spent engine-oil (which was the most effective treatment). While neem seed oil was significantly (p 0.05) in the population index of ants that emerged from nests treated with aqueous neem seed extract, salt/potash solution and extract of Eugenia aromatic fruit from those of the control. Experiment 2 result showed no significant difference (p 0.05) from the untreated control. While the result shows no significant difference between the treatments, it was observed that equal mixture of Spent Engine-oil and Neem Seed Kernel Oil was most effective, followed by Spent Engine-Oil, Permethrin and Neem Seed Kernel Oil, in that order.

Association mapping seed kernel oil content in upland cotton using genome-wide SSRs and SNPs

Cotton is a major fiber and oilseed crop; however, few studies have been focused on oil content in cotton. To dissect the genetic basis of seed oil content (SOC), genome-wide association study was performed by using SOC phenotypic data of 503 upland cotton inbred accessions in eight environments and two sets of genotypic data including 179 simple sequence repeats (SSRs) and 11,975 single-nucleotide polymorphism (SNPs) from our previous study. SOC for a given genotype was fairly stable which was reflected in a broad-sense heritability of H2 = 96.6%. Using the mixed linear model (K + Q), 16 significant SSRs (P < 0.01) and 26 significant SNPs (P < 1/11,975), were detected cross BLUP values and at least one individual environments. Based on the LD block analysis, eight SOC-associated QTL on chromosome 6, 10, 12, 13, 15, 17, and 24 covering more than one significant markers were identified, two QTL of them on chromosome 10 and 13 could be matched between SSR and SNP markers. The confidence interval of candidate genes in each QTL ranged from 0.14 to 1.50 Mb, and 12 to 70 genes were identified in these QTL. According to the gene function annotation and gene FPKM value of the 20~35 days post anther (DPA) cotton ovules, key genes involved in lipid metabolism and transport; tricarboxylic acid cycle which might affect seed oil content were found near the leading markers. These results will facilitate the molecular marker assisted breeding of cotton oil content.

Physico-chemical Characteristics of Washingtonia robusta Fruit Oil

The fruit seed kernel oil from Washingtonia (W. robusta) locally in Aswan governorate, Egypt were evaluated for their physicochemical properties, and composition of fatty acids, tocopherols and phytosterols. The contents of carbohydrates, fiber, protein and ash for W. robusta palm fruits were 47.00, 6.50, 5 and 4.2%, respectively. The oil content in the seed kernels was 32.80%. The extracted oil had as refractive index (at 20oC) 1.46, specific gravity (at 20oC) 0.92, colour (yellow, 3.5; red 0.09), average iodine value (g of I/100g oil) 87.68; acid value (% oleic acid) 0.22%; saponification value (191.66 mg of KOH/g oil; unsaponifiable matters 0.70, the oxidation stability was 30. The major fatty acids detected in oil were noted to be oleic acid (64.80%) followed by palmitic acid (14.40%), linolenic acid (5.20%), arachidic acid (5.10%), linoleic acid (4.50), stearic acid (4.30) and myrstic acid (1.70%). The observed data showed that the total saturated fatty acids 25.50%, whereas the amounts of total unsaturated fatty acids 74.50%.The content of total tocopherols in oil was 97.4 mg/100g), with α-tocophero l16.0, γ-tocophero l31.4, and δ-tocopherol (50 mg/100g). β-sitosterol (11.331 mg/100 g) the main phytosterols in oil. The results of this study revealed that the Washingtonia robusta fruit could be explained as a potential source of high-oleic oil for the local oil industry as well as the seeds is a nutritive value.

Physicochemical Properties of the Seed Kernels and the Oil of Custard Apple (&amp;lt;i&amp;gt;Annona squamosa&amp;lt;/i&amp;gt; L.)

Custard apple (Annona squamosal L.) seed kernel and the extracted oil were characterized for their physicochemical properties. Crude ether extract was found to be the main component where, the seed kernels had 31.22%. Moreover, protein content was 20.01%. On the other hand, the crude fiber and total ash were 15.43 and 1.89%, respectively. Total phenolic compounds, antioxidant activity and IC50 of CASKF were 42.02 mg GAE/ 100g, 87.55% and 22.84 μg/ml, respectively. The results indicated that CASKF is rich in content of K, P, Ca, Mg and Na. Nevertheless, very low levels of Cd and Pb were detected. The amino acid composition of the defatted CASKF indicated that glutamic, aspartic, alanine, leucine and arginine were the predominant amino acids. The total amount of essential amino acids in the defatted CASKF was 37.77 g /100g protein (-) which is higher than that reported in FAO/ WHO pattern. The dominant fatty acids of custard apple seed kernel oil were oleic (49.75%), Linoleic (22.50%), palmitic (15.06%) and stearic and (4.63%). The oil could be classified as a semi-dry oil. Total lipid fractions consisted mainly of nine classes in which triacylglycerols were the major class.

Screening of fatty acids, saccharides, and phytochemicals in Jatropha curcas seed kernel as their trimethylsilyl derivatives using gas chromatography/mass spectrometry

Jatropha curcas is a multipurpose plant, of which the seed kernel oil (up to 60% content) has been exploited for BDF production. In this report, we explored the various kinds of minor compounds of saccharides, phytochemicals, fatty acids (FAs), and amino acids in the seed kernel using gas chromatography/mass spectrometry (GC/MS) as their trimethylsilyl (TMS) derivatives. The homogenized seed kernels were extracted with methanol, and the extract was distributed into ethyl acetate/water phase. The components of each layer were derivatized with N, O-bis (trimethylsilyl) trifluoroacetamide (BSTFA) and their TMS derivatives were screened by GC/MS analysis. In ethyl acetate layer, the four FAs of palmitic acid, oleic acid, linoleic acid, and stearic acid were identified with total content of 12 wt% in kernel. In addition, the two tocochromanols of γ-tocopherol and γ-tocotrienol, and three phytosterols of campesterol, stigmasterol, and β-sitosterol were also identified. Meanwhile, as the main saccharide components, di-saccharide of sucrose with content of 3 wt% in kernel, tri-saccharide of raffinose, and sugar alcohol of sorbitol and myo-inositol, were identified in aqueous layer. Furthermore, metabolites of amino acid, and a series of metabolite were also identified. These results suggested that the Jatropha curcas seed kernel can be applied to cascade use for metallic soap, liquid fuel, food and medical supplement, and cosmetics in addition to biodiesel production.

Mechanical characteristics and grinding studies of mango seed kernel

Mango kernel seed oil is rich in unsaturated fatty acid, bioactive compounds and a potential source of butter production. This study explores the unit operation of size reduction which is a vital step in the production of mango seed kernel flour and hence, mango seed kernel oil. The mechanical property of mango seed kernel, its grinding characteristics using hammer mill and then particle size distribution (PSD) of its flour were evaluated. Comparison was made of the PSD using laboratory sieve shaker and laser diffraction technique. The Kick's model had the best energy efficiency followed by Bond's and Rittinger's models respectively. PSD by Laser diffraction method indicated higher mass relative span (RSm), Inclusive graphic skewness (IGS), kurtosis of PSD (Kg) and graphic means. Rosin-Rammler-Bennet (RRB) and Gaudin-Schuhmann (GS) models were effectively used to describe the statistical and model parameters of the particle size distribution data.

Evaluation of biodiesel from Xanthoceras sorbifolia Bunge seed kernel oil from 13 areas in China

Oil content from seed kernels of Xanthoceras sorbifolia from 13 areas in China was analyzed by gas chromatography–mass spectrometry to determine oil characteristics and biodiesel properties. The seeds had a high kernel percentage (53.67% ± 7.51), oil content (52.21% ± 4.01), and biodiesel yield (99.77% ± 0.21). Among the fatty acids in the oil were high percentages of linoleic acid (41.66 ± 2.26)% and oleic acid (28.44% ± 2.03). Most of the fuel properties complied well with the ASTM D6751-10, EN 14214-08, and GB/T 20828-2014 standards. The 13 sampling areas were grouped into four clusters based on different kernel percentage, oil content, biodiesel yield, and fatty acid composition. The results showed that the quality of kernel oils from seeds from Ar Horqin Banner was the best, although kernel oils from seeds in all 13 areas were suitable for biodiesel production. This study provides a basis for selecting optimal sites to harvest seeds from X. sorbifolia.

Synthesis of Methyl Esters from Silk Cotton Tree Seed Kernel Oil Using Dimethyl Carbonate and KOH Catalysis

Silk cotton seed kernel oil is given as a new source for methyl ester synthesis. Crude silk cotton oil was used as feedstock raw materials for methyl ester production using dimethyl carbonate as solvent and KOH catalysis. The maximum methyl esters yield produced as 97% with a kinematic viscosity (4.25 ± 0.2 5 mm2/sec) was reached at 80 °C by boiling a mixture of dimethyl carbonate (DMC) and oil mole ratio as 8:1 with 1.5 wt.% KOH catalyst (oil weight based ) for 75 min. The produced products were analyzed using gas chromatography-mass spectrometry to identify the methyl esters. The properties of the methyl esters from silk cottonseed kernel oil produced met the specifications of ASTM for methyl esters. The kinetics of the KOH-catalyzed transesterification of diglyceride (DG) and triglyceride (TG) with DMC were studied at 40 to 90 °C. We found that the activation energies for transesterification of diglycerides and triglycerides were 89.8 and 83.3 kJ/mol, respectively. The results showed that all the reaction variables studied had beneficial effects.

Effect of extraction methods on physicochemical, nutritional, antinutritional, antioxidant and antimicrobial activity of Moringa (Moringa oleifera Lam.) seed kernel oil

The effect of three different extraction methods, namely supercritical CO2, soxhlet and solvent methods on the yield, efficiency, physico-chemical properties, nutritional, anti-nutritional composition, antimicrobial and antioxidant activities of moringa (Moringa oleifera Lam.) seed kernel oil was investigated in this study. Oil extraction for SC-CO2 were 37.76 g/100g and 98.43%, observed to be higher than those of soxhlet extraction (29.12 g/100g and 76.29%), and significantly lower than solvent extraction (22.12 g/100g and 57.99%). The physico-chemical composition of the oils showed considerable variation among the extraction methods. The SC-CO2 extracted oil was found to be of superior quality, showing negligible thermal degradation and exhibited significantly (p&lt;0.01) higher nutritional and antioxidant activity and lower anti-nutritional composition than the soxhlet and solvent extracted oils. Oils produced by SC-CO2 and soxhlet extraction methods had antimicrobial activities higher than solvent extracted oil. SCCO2 extracted oil was found to have maximum number of bioactive compounds (14 compounds) followed by solvent (8 compounds) and soxhlet (4 compounds) extracted oil. The results of the study demonstrated that SC-CO2 would be a promising process for the extraction of moringa seed kernel oil of premium quality.

Association of Fruit and Seed Traits of Sapindus mukorossi Germplasm with Environmental Factors in Southern China

Sapindus mukorossi is widely distributed in tropical and subtropical areas of southern China; the seed kernel oil is potential biodiesel material, and the saponins extracted from fruit pericarp are very valuable efficient natural surfactants. Therefore, S. mukorossi is an ideal tree species for developing forestry bioenergy and multiple other products. In this study, 42 S. mukorossi fruits from mother trees were collected from 39 distinct locations in 12 Chinese provinces to infer fruit and seed trait responses to environmental factors. Canonical correspondence analysis (CCA) was conducted using 21 horticultural fruit traits and 10 environmental factors that represented different climatic and geographic conditions throughout southern China. CCA revealed well-developed patterns of natural phenotypic variation, and insight into the ecological factors that are potentially important in shaping this variation. The results presented here further elucidate the natural distribution and ecological adaptations of wild S. mukorossi resources, which will be valuable for S. mukorossi cultivation by helping identify ideal planting areas. The germplasm resources with extensive morphological variation can also contribute to S. mukorossi breeding in the future by helping develop new cultivars with high saponin yield.

Optimization of mango seed kernel oil extraction using response surface methodology

Response surface methodology (RSM) was applied to study the optimum condition of mango seed kernel oil (MSKO) extraction with n-hexane. In the present paper, for the first time, we provide an optimal condition of MSKO extraction to obtain the maximum of yield, total phenolic content (TPC) and vitamin E. The experiment was conducted using central composite design (CCD) consisted of twenty experimental points including six replicates of center points to study the effect of three independent variables: temperature, time and amount of n-hexane on dependent variables. Data were analyzed using Design-Expert 10 software. The result showed that the optimum condition of MSKO extraction were 54.19 °C, 5.24 h and 224.23 mL of n-hexane. In this condition MSKO have yield of 7.03%, TPC of 67.77 mg GAE/g oil and vitamin E of 141.22 mg/L. These results suggest that MSKO has potential as raw materials of food products oil-based and as a natural antioxidant of functional food and for use in food processing.

Mutagenesis and characterization of a Bacillus amyloliquefaciens strain for Cinnamomum camphora seed kernel oil extraction by aqueous enzymatic method

The purpose of the present study was to increase the proteinase activity of the strain NCU116 by combining ultraviolet irradiation and N-methyl-N′-nitro-N-nitroso guanidine treatment, in order to enhance the efficiency of Cinnamomum camphora seed kernel oil (CCSKO) extraction by aqueous enzymatic method (AEM). The mutated strain, designated as NCU116-1, was screened out by the ratio of hydrolytic zone diameter to colony diameter on skim milk plate. The proteinase activity (9116.1 U/ml) of NCU116-1 was increased by 31.9% compared with the parental strain. The extracellular enzymes produced by NCU116-1 included proteinase, pectase, glucoamylase, cellulase and amylase. The proteinase had the maximum activity at 50 °C. Its optimum temperature and pH value were approximately 45 °C and 8.0 respectively. Mn2+ was an activator of neutral proteinase. The glucoamylase had the maximum activity at 35 °C, and was activated by Cu2+, Fe3+ and Mn2+. Its optimum temperatures and pH value were 35 °C and 8.0 respectively. The pectinase had the maximum activity at 40 °C, and was activated by Ca2+ and Mn2+. Its optimum temperatures and pH value were 35–40 °C and 6.0 respectively. The optimum conditions of CCSKO extraction by AEM were also investigated. The results suggested that the best amount of enzyme solution and enzymolysis time were 20% (v/v) and 4 h, respectively. The oil extraction rate was 95.2% under these conditions. Thus, a suitable mutated strain was selected for CCSKO extraction by AEM and the optimum extraction conditions were determined.

Characterization of seed kernel oil of Bangladeshi mango and it’s evaluation as cosmetic ingredient

The physicochemical properties, fatty acid composition, aflatoxin contamination and heavy metals of extracted mango kernel oils of four Bangladeshi varieties (Langra, Gopalbhog, Khirshapat &amp; Amrupaly) were studied. The results were compared with published values of ‘CIR expert panel’, ‘Indian Standard of Mango Kernel Fat’, ‘Scientific Commission of the European Community’ and ‘US Food and Drug Administration (FDA)’. The estimated values of aflatoxin B1and total aflatoxins were 1.45809 ppb and 5.14761ppb respectively which were lower than the limit levels of ‘European Community (2 ppb for aflatoxin B1)’ and ‘US FDA (20ppb for total aflatoxins value)’. Heavy metals like arsenic &amp; lead concentration were found 0.034 &amp; 0.45 ppm respectively which were lower than the value of ‘Indian Standard of Mango Kernel Fat; 9231 (1979)’ (0.5 &amp; 5.0 ppm respectively). In fatty acid composition; palmitic acid, stearic acid, arachidic acid, oleic acid and linoleic acid were within the range of ‘CIR expert panel’ reported values.Bangladesh J. Sci. Ind. Res. 52(1), 43-48, 2017

Natural variation in fatty acid composition of Sapindus spp. seed oils

Sapindus mukorossi and Sapindus delavayi seeds were collected from 60 populations distributed over a wide geographic area (a range of 12.0° latitude, 19.0° longitude, and 1800m elevation) across southern China. The variation in fatty acid composition of seed kernel oil between the two species and among individuals was determined, and relationships with geographical and environmental factors were analyzed. The oil characterization allowed the identification and the quantification of 12 fatty acids. The C16–C20 fatty acids accounted for more than 98% of the total content, of which oleic acid was the most abundant (more than 50%). The fatty acid composition was generally stable between the two species and among individuals despite the wide geographic range from which the samples were collected, and the oil showed good potential for biodiesel production. Oleic acid showed insensitivity to environmental factors. Correlation analysis showed that environmental factors had a strong influence on the composition of other fatty acids, such as C20:1 (20.95%), C18:2 (7.56%), C20:0 (6.01%), and C16:0 (4.55%), and explained 36.68% of the total variance. Among the environmental factors, elevation, maximum temperature, and precipitation significantly contributed to variation in seed kernel oil fatty acid composition among populations. Furthermore, the cooperativity of elevation and maximum explained 30.13% of the variance (P<0.01). Thus, environmental factors, especially elevation, maximum temperature, and precipitation, of the original provenance should be considered during genotype selection.

Cost analysis of biodiesel from kernel oil of tea seed

ABSTRACTCurrently, biodiesel is mainly produced from conventionally grown edible oils such as soybean, rapeseed, sunflower, and palm. To obtain biodiesel from edible oils is currently not economically feasible to substitute petroleum diesel. Biodiesel was obtained from kernel oil of tea seed (Camellia sinensis L. Kuntze). The predominant fatty acid was oleic acid (62.5% wt) in the tea kernel oil, followed by linoleic acid (18.1% wt). The seed kernel oil was transesterified to methyl esters using KOH catalytic methanol. The tea kernel oil has lower pour point and lower viscosity compared with common vegetable oils. Lower-cost raw materials are needed since biodiesel from food-grade oils is not economically competitive with petroleum-based diesel fuel. Biodiesel production costs can vary widely by feedstock, conversion process, scale of production, and geographic region. The cost of feedstock is a major economic factor in the viability of biodiesel production. Feedstock costs typically account for 60–80% of the total costs of biodiesel production. The crude tea kernel oil is one of the cheapest raw materials for biodiesel production.

Screening and identification of a Bacillus amyloliquefaciens strain for aqueous enzymatic extraction of medium-chain triglycerides

A strain with a high yield of neutral proteinase and low yield of lipase, resistance to medium chain fatty acid triglycerides is the key to increasing yield and quality of Cinnamomum camphora seed kernel oil (CCSKO) with aqueous enzymatic extraction technology. A bacterial strain, NCU116 isolated from the waste residue produced in CCSKO production through primary screening with plate and secondary screening with shake-flask fermentation. It was found to be suitable for the extraction of CCSKO or other medium-chain triglycerides by using the extraction technology. Its activity of neutral proteinase was 4536.5 U/mL, and only 0.088 U/mL for lipase production. The strain was identified as Bacillus amyloliquefaciens by morphological, physiological, biochemical and 16S rDNA molecular identification. The extracellular enzymes produced by NCU116 included neutral proteinase, pectinase, glucoamylase, cellulase, amylase and lipase. The neutral proteinase had the maximum activity at 50 °C, but was unstable. Its optimum temperature and pH value were approximately 40 °C and 7.0 respectively. Mn2+ was an activator of neutral proteinase. The glucoamylase had the maximum activity at 45 °C, and was activated by Ca2+, Zn2+, Fe3+ and Mn2+. Its optimum temperatures and pH value were 45–50 °C and 6.0 respectively. The pectinase had the maximum activity at 40 °C, and was activated by Cu2+, Fe2+, Mn2+ and Ca2+. Its optimum temperatures and pH value were 35–40 °C and 7.0 respectively. The cellulase had the maximum activity at 35 °C, and was activated by Ca2+ and Mn2+. Its optimum temperatures and pH value were 30–40 °C and 7.0 respectively.

Physicochemical and Phytochemical Characterization of Seed Kernel oil From Desert Date (Balanites Aegyptica)

Physicochemical and Phytochemical Characterization of Seed Kernel oil From Desert Date (Balanites Aegyptica)

Evaluation of apricot (Prunus armeniaca L.) seed kernel as a potential feedstock for the production of liquid bio-fuels and activated carbons

Production of liquid bio-fuels (biodiesel and bio-oil) as well as activated carbon from one non-edible feedstock, apricot (Prunus armeniaca L.) seed kernel was the main objective of the present research work. The oil was extracted from apricot seed kernel with a yield of 49.44% w/w of kernels. Potassium hydroxide-catalyzed transesterification of apricot (Prunus armeniaca L.) seed kernel oil with methanol and ethanol was then applied to produce methylic and ethylic, respectively. Properties of the obtained biodiesels were evaluated and found conformed to ASTM D 6751 limits. The apricot de-oiled seed kernel was pyrolyzed in a semi-batch reactor for bio-oil production. The effect of the pyrolysis temperatures (350, 400, 450, 500, 550 and 600°C), pyrolysis time (30, 60, 90, 120 and 150min) and feed particles size (0.25, 0.40, 0.59 and 0.84mm) on the bio-oil yield was investigated. The maximum production of bio-oil (43.66% w/w) was achieved at a pyrolysis temperature of 450°C, 60min pyrolysis time and a feed particles size of 0.25mm. The bio-oil obtained under the optimal conditions was characterized by the elemental analysis, FTIR spectroscopy and column chromatography. The FTIR analysis of the produced bio-fuel indicated that it composes mainly of alkanes, alkenes, ketones, carboxylic acids and amines. Properties of the resulting bio-oil were analyzed in terms of calorific value, density, flash point, pH, acid value, pour point and refractive index. The properties were close to those of petroleum fractions and comparable to those of other bio-oils published in literature. Referring to the experimental results, the obtained bio-oil can be utilized as an important source of alternative fuel and chemicals. The chemical activation method by using sodium hydroxide as the activating agent was utilized to convert the bio-char into activated carbon. The effect of the process parameters including the activation temperatures (400, 500, 600, 700 and 800°C), activation time (30, 60, 90, 120 and 150 min) and feed particles size (20, 30, 40 and 60 mesh size) on the yield, iodine adsorption number and surface area of the obtained activated carbons were investigated. The optimal activated carbon sample was obtained at 600°C activation temperature, 90min activation time and a feed size of 60 mesh. The resulting activated carbon showed good characteristics to be used as adsorbent in water purification as indicated by scanning electron microscope and FTIR spectroscopy.

Cinnamomum camphora Seed Kernel Oil Improves Lipid Metabolism and Enhances β3-Adrenergic Receptor Expression in Diet-Induced Obese Rats.

The effects of dietary Cinnamomum camphora seed kernel oil (CCSKO) containing medium-chain triacylglycerols on lipid metabolism and mRNA and protein expression of β-3 adrenergic receptor in adipose tissue were studied in diet-induced obese rats. High fat food-induced obese rats were randomly divided into CCSKO group, Lard group, Soybean oil (SOY) group and naturally restoring group (n=10). Rats fed with low fat food were used as a normal control group. Significant decreases in body mass and abdominal fat mass/body mass after 12weeks were found in CCSKO group as compared with Lard and SOY groups (p<0.05). Levels of blood total cholesterol (TC), triglyceride, free fatty acid, fasting insulin and insulin resistance in the CCSKO group were decreased significantly, and noradrenaline level and insulin sensitivity index in the CCSKO group were significantly higher than other groups. Meanwhile liver TC and triglyceride levels in the CCSKO group were also decreased markedly. Expression levels of β3-adrenergic receptor mRNA and protein were higher in CCSKO group than in Lard and SOY groups. These results suggest that CCSKO may contribute to reduction of the body fat mass, promote lipid metabolism and up-regulate β3-adrenergic receptor expression in high fat diet-induced obese rats.

Cinnamomum camphora Seed Kernel Oil Ameliorates Oxidative Stress and Inflammation in Diet-Induced Obese Rats.

Cinnamomum camphora seed kernel oil (CCSKO) was found to reduce body fat deposition and improve blood lipid in both healthy and obese rats. The study was aimed to investigate the antioxidative stress and anti-inflammatory effects of CCSKO in high-fat-diet-induced obese rats. The obese rats were treated with CCSKO, lard, and soybean oil, respectively, for 12 wk. The level of total antioxidant capacity (T-AOC), activities of superoxide dismutase (SOD), glutathione peroxidase, and catalase, and levels of malondialdehyde (MDA), tumor necrosis factor (TNF)-α, peroxisome proliferator-activated receptor (PPAR)-γ, interleukin (IL)-6, and P65 were compared among CCSKO, lard, and soybean oil groups. Our results showed that the level of T-AOC and activities of SOD and catalase were significantly increased and the level of MDA was significantly decreased in CCSKO group. In addition, CCSKO treatment reduced the activities of serum glutamic oxaloacetic transaminase and glutamate-pyruvate transaminase, and levels of serum TNF-α, IL-6, and P65 through raising the level of PPAR-γ. In conclusion, CCSKO has, for the first time, been found to ameliorate oxidative stress and inflammation in high-fat-diet-induced obese rats.