Second Year Of Vegetation Research Articles

Chemical Components and Biological Activities of Essential Oils of Mentha × piperita L. from Field-Grown and Field-Acclimated after In Vitro Propagation Plants

In this work, we studied in vitro propagation of three cultivars of Mentha × piperita L. Murashige and Skoog medium (MS) supplemented with 0.5 mg·L−1 BAP was the most optimal medium for micropropagation of the cultivars studied. The ability of peppermint plants field-acclimated after in vitro micropropagation to produce essential oils (EOs) was investigated. EO was obtained by hydrodistillation from dried leaves and flowering shoots from control (field grown) plants and plants acclimated in field conditions after in vitro propagation. The samples were collected at the first and second year of vegetation, and their chemical composition was investigated using gas chromatography-mass spectrometry (GC-MS). Differences were observed in the yield, as well as in the quantitative and qualitative composition of the EOs extracted from the control plants and field-acclimated plants after in vitro propagation. Menthol was the main component of the EO in control plants, while pulegone and menthone were dominant in the EO pattern in field-acclimated in vitro regenerants in the first year of the growing season. However, in the second year of vegetation, the content of the main EO components in field-acclimated peppermint plants was approximately the same as in control plants. The antioxidant activity of EOs extracted from field-acclimated after in vitro micropropagation plants was found to be the same as in control field-grown M. × piperita plants.

Prospects and methods of polyploid selection of new high-yielding miscanthus clones in the natural climatic conditions of Ukraine

Goal. Expanding the breeding trait collection of breeding genotypes of the genus Miscanthus (Anderson) and the genetic basis for new allotriploid clones by transferring components for hybridization of natural wild species Miscanthus sinensis and Miscanthus sacchariflorus to the tetraploid level.
 Methods. Cytological, biotechnological, fluorescent cytphotometry, field, laboratory.
 Results. The efficiency of polyploidisation for induction of new tetraploid forms of miscanthus in liquid nutrient media supplemented with colchicine (0.05% mass) and for stabilization of myxoploid shoots (0.005% mass) for 6 h of cultivation is examined. The period of exposure for Miscanthus sinensis (2x=2x= 38) and Miscanthus sacchariflorus (2x = 2x = 38) varied from 2 hours to 3 days depending on the genetic origin of the material, with transfer to a hormonal environment. The best indicators of tetraploid induction for Miscanthus sinensis were observed for the exposure period of 2 days with polyploidization efficiency (Db%) of 31.25% and 21.42%, and for Miscanthus sacchariflorus 2 and 6 hours with 35.0% and 27.3%, respectively. The technology of transfering rooted shoots into the soil on the Yaltushkiv Eperimental Breeding Farm was improved and the composition of the soil mixture that provided 99% rooting of culture seedlings at a humidity of 60−70% and air temperature of 35−55°С was found.
 Conclusions. New biotechnological tetraploid lines of Miscanthus sinensis (2xn=4x=76) and Miscanthus sacchariflorus (2xn=4x=76) were created in the conditions of liquid nutrient media supplemented with colchicine (0.05 % mass). It is investigated that flowering of new tetraploid clones in the conditions of Ukraine for the second year of vegetation occurs from late September to early October with formation of fertile pollen grains.

Features of Trifolium rubens (Leguminosae) ontomorphogenesis in conditions of Ukraine

The necessary basis for studying the structure and dynamics of populations is an investigation of ontomophogenetic peculiarities and establishment of ontogenetic stages of individuals. Information about ontomorphogenesis is especially important for protected species of plants. The minimal amount of data on morphology of plants in the different periods of ontogenesis complicates plant identification and evaluation of population state conditions in nature and culture. Trifolium rubens L. is a perennial Central European plant listed in the Red Book of Ukraine as a rare species. The objective of this research is to analyze the peculiarities of ontomorphogenesis of T. rubens, to establish diagnostic signs of age stages and the sequence of their passage for identification of the multivariance of development. The results of the study on T. rubens ontomorphogeny at the M. M. Gryshko National Botanical Garden оf the National Academy of Sciences of Ukraine and in the natural population in Zakarpattya are represented in this article. The terms “age stage” and “age period” are used to describe the degree of ontogenetic development of individuals. Diagnostic features of the age periods: latent, pregenerative, generative and senile are established. Detailed morphological descriptions of plants in different stages of ontogenesis are presented. The study shows a multivariate development that reveals different sequences of passing ontogenesis stages by individuals and, accordingly, the rate of their aging and life expectancy. It was found that during the first year of vegetation the plants undergo two periods (latent and pregenerative) and 5 stages of ontogeny (latent, seedlings, juvenile, immature and virginile). The first bloom in plants appears mainly in the second year of vegetation. The research showed the change of the leaf blade shape in ontogeny from simple to compound. There is a difference in the pubescence of leaf parts, and leaf shape indicates that these features are age-old. It was concluded that the aboveground part of the immature, virginile, generative individuals during the flowering period of plants differ from subsenile and senile in shape and number of leaves. In the generative period of ontogeny in dense plantings or in the natural population, it is difficult to reliably determine the boundaries of an individual by the aboveground part. The correlation between vegetative shoots of different type and generative shoots in the individual or group of plants can be used as an indicator to estimate the ontogenetic status of a particular locus or population. Successful growth of T. rubens in culture outside the natural range indicates good prospects for its ex situ protection and the implementation of measures for repatriation and restoration of populations in nature. The data of the research may be used for evaluation of the age structure of T. rubens populations.

Productivity of common Saint-John's wort (Hypericum perforatum L.) by using transplant reproduction method in the conditions of drip irrigation

The influence of plant nutrition area and mineral fertilizer rates on the productivity of St. John's wort (Hypericum perforatum L.) by using transplant reproduction method in the conditions of drip irrigation was studied. It was proved that the transplant method of cultivation of St. John's wort under drip irrigation is a very effective method of reproduction of this crop. Four variants of planting density per unit area were studied: 42 thousand plants / ha (cultivation scheme 60x40 cm), 56 thousand plants / ha (60x30 cm), 83 thousand plants / ha (60x20 cm) and 167 thousand plants / ha (60x10 cm). Yield recording of raw materials (air-dry tops) was carried out in the phase of mass flowering. In the first year of vegetation this period was in the first decade of August, in the second year – in the second decade of June. It was found that the increase in the number of planted plants of St. John's wort per 1 ha contributed to the increased plantation productivity. When having a cultivation plant density of 42,000 plants / ha, the yield of dry grass in the first year of vegetation was 3,02 t / ha. Increasing the number of plants to 56 thousand plants / ha provided 3,26 t / ha of raw materials. The highest yield of dried St. John's wort – 3,76 t / ha in the first year of vegetation was obtained in the variant with the largest number of planted plants per unit area - 167 thousand plants / ha. In the second year of vegetation of St. John's wort in the variant with the lowest plant density of 42 thousand plants / ha, the yield was 3,65 t / ha. The most productive plantation of the second year of vegetation was in the variant with a plant density of 83 thousand plants / ha, where the yield of dry raw materials was 3,96 t / ha. A further increase in the number of plants per unit area led to a decrease in crop yields. The influence of four variants of the main application of mineral fertilizers on the productivity of St. John's wort was also studied: N0P0K0 (reference), N60P60K60, N120P120K120 and N180P180K180. It was found that with increasing fertilizer application rate, the yield of dry raw materials increased. The most favorable conditions for growth and development of plants of St. John's wort developed in the variant with the maximum rate of fertilizer application - N180P180K180, where the yield of dry raw materials in the first year was 3,31 t / ha, and in the second year – 4,15 t / ha, which exceeded the reference result (without fertilizers) by 0,61 t / ha and 0,84 t / ha, respectively.

PECULIARITIES OF CREATION OF MISCANTHUS SINENSIS AND MISCANTHUS SACCHARIFLORUS TETRAPLOID LINES

The work aimed at creating tetraploid lines of Miscanthus sinensis and Miscanthus sacchariflorus species. To achieve this goal, we used methods of microclonal propagation, fluorescence cytophotometry, and genomic status differentiation using computer software of AP ‘Partec’ (Germany). It was found that the percentage of cultivated shoots of Miscanthus sacchariflorus for an exposure period of 1 day was 18.86 ± 5.37%. To compare, it was51.78 ± 6.51% in Miscanthus sinensis. The best indicators of tetraploid induction in Miscanthus sinensis were observed for the exposure to colchicine for 2 days with polyploidization efficiency of 31.25% and 21.42%, and in Miscanthus sacchariflorusf or 2 hours and 6 hours with rates of 35.0% and 27.3%, respectively. To stabilize the tetraploid level of genome ploidy, we used Murashige and Skoog liquid media (1962) supplemented with 0.005% colchicine and an exposure period of myxoploids for 6 hours. The flowering of new tetraploid clones in the conditions of Ukraine was observed on the second year of vegetation in late September and the beginning of October with the formation of fertile pollen grains. However, development of a microgametophyte depends on temperature conditions, both for Miscanthus sinensis (4x) and Miscanthus sacchariflorus (4x). Breeding schemes for the formation of anisoploid populations have also been developed: M sinensis (4x) x M sinensis (2x); M. sa?chariflorus (4x) x M sinensis (2x); M sinensis (4x) x M. sachariflorus (2x)

Efficiency of mineral fertilizers for growing Althaea officinalis L. under conditions of irrigation

In the Forest-Steppe Zone of Ukraine, a study was carried out to establish the effect of mineral fertilizers and drip irrigation on the yield of dry raw material. It was found that irrigation significantly increases productivity of althaea, by eliminating the deficit of soil moisture in critical periods of growth and development. The yield increase caused by the use of drip irrigation in the first year of vegetation of the crop was 1.27–1.98 t/ha for dry grass, for leaves — 0.69–1.17 t/ha, for roots 0.22–0.39 t/ha. For the second growing season, the yield increase was: 1.52–4.7 t/ha for grass, 0.46–1.35 t/ha for leaves and 1.5–2.76 t/ha for roots. It was also found that increase of the mineral fertilizers main application dose caused increase of the yield. The highest yield of dry raw material of the culture was obtained in both the first and second years of the growing season with the introduction of N180P180K180. With the application of mineral fertilizer at a dose of N180P180K180 under conditions of drip irrigation, the yield of dried althaea grass was 8.09 t/ha, leaves — 3.88 t/ha, roots — 3.08 t/ha, which exceeded the option without fertilizers and without irrigation by 4.08 t/ha for grass, 2.04 t/ha for leaves, 1.44 t/ha for roots. The highest yield was obtained in the second year of vegetation using the variant with the application of the highest dose of mineral fertilizers under drip irrigation, where the yield of dry grass was 15.78 t/ha, leaves — 4.42 t/ha, roots — 6.24 t/ha. On the plantation of the second year of vegetation in the variant without irrigation and without fertilizers, the yield of raw materials was the lowest. It was 6.85 t/ha for grass, 1.88 t/ha for leaves and 2.12 t/ha for roots.

IMPROVEMENT OF ELEMENTS OF TECHNOLOGY OF GROWING MILLET IN THE CONDITIONS OF THE RIGHT BANK FOREST BANK

The height of millet rods depended more on the year of cultivation, the plants of the second year of vegetation, regardless of the studied factors provided higher linear measurements of plant height from 108.5 to 152.2 cm in comparison to plants of the first year of cultivation from 64.1 to 70.2 cm. higher linear measurements of plant height were obtained at a row spacing of 30 cm, both in plants of the first and second year of vegetation. In plants of the first year of vegetation from 65.2 to 70.2 cm, and in plants of the second year of vegetation from 123.5 to 152.2 cm. Lower linear measurements of plant height were observed for row spacing 45 cm from 64.1 to 69.4 cm in plants of the first year of vegetation and from 108.5 to 138.3 cm in plants of the second year of vegetation. In plants of millet twigs there are some differences in forming a number of stems depending on the year of vegetation, in plants of the first year of vegetation the highest number of stems was 30 cm wide between rows and varied from 405.1 to 443.3 pieces / m2. However, in the plants of the second year of the growing season, the highest number of stems was obtained with a row spacing of 45 cm and varied from 457.5 to 488.1 units / m2. Moreover, the highest number of stems was obtained in plants of millet twigs for sowing in the first decade of May - 429.8 and 442.6 pieces / m2 in the varieties Cave-in-Rock and Foresburg. The highest level of dry biomass yield was obtained for row spacing of 45 cm from 7.0 to 8.2 t / ha, and for row spacing of 30 cm from 6.7 to 7.5 t / ha. Plants of the second year of vegetation provided a higher level of dry biomass yield, which varied from 6.9 to 8.2 t / ha. Dry biomass yields were also affected by sowing dates, the highest mass of dry biomass was obtained during sowing in the first decade of May in Cave-in-Rock varieties - 5.7, and in Foresburg variety - 6.0 t / ha.

WATER CONSUMPTION BY AGROECOSYSTEMS OF LEGUMINOUS PERENNIAL GRASSES DEPENDING ON VEGETATION YEARS

At the end of the growing season of the first year of life of perennial legumes, the highest soil moisture - 27.45% was observed in the agroecosystem of alfalfa, and the lowest - 24.14% - in the agroecosystem of the horned lily. The coefficient of water use by agroecosystems of legumes of perennial grasses in the first year of their growing season was 455.1-1738.0. The smallest coefficient of water use was in the agroecosystem of the white melilot, and the highest in the eastern goat's rue. At the end of the growing season of perennial legumes of the second year of life, soil moisture in agroecosystems was 11.67-17.00%. It was the largest in the agroecosystem of white melilot, which forms only one cut in the middle of summer in the second year of its life and then dies off, and the smallest in the agroecosystem of sandy sainfoin. The coefficient of water use by agroecosystems of perennial legumes in the second year of vegetation was 429.2-696.7. It was the smallest in the agroecosystem of white melilot, and in perennial legumes that grow by autumn - in the agroecosystem of sandy sainfoin, and the highest was in horned beetle. By the end of the third year of the growing season of perennial legumes, soil moisture had been 18.00-23.11%. It was the largest in the agroecosystem of sandy sainfoin, and the smallest in the horned sainfoin. The coefficient of water use by agroecosystems of leguminous perennial grasses for the third year of growing season was 484.0-619.6. The smallest was in the agroecosystem of the sandy sainfoin, and the largest was the horned sainfoin. At the end of the fourth year of the growing season of perennial legumes, soil moisture was valued in 22.60-25.07%. It was the largest in the agroecosystem of sandy sainfoin, and the smallest in alfalfa. The coefficient of water use by agroecosystems of perennial legumes in the fourth year of their growing season was 489.3-1130.7. Less moisture is used for the formation of a unit of dry matter by the agroecosystems of sainfoin, the coefficient of water use of the agroecosystem of alfalfa is higher by 13.4%. The agroecosystem of Lyadvenets horned had the highest coefficient of water use - 2.3 times more than in the agroecosystem of sainfoin. In conditions of the greenhouse effect, in particular, with an increase in air temperature, an increase in moisture evaporation from the soil and a decrease in the amount of atmospheric precipitation, it is necessary to grow herbs that consume moisture most economically and have the lowest water consumption coefficient: the first year of growing season - white melilot - 455.1; second year - white sweet clover - 429.2 and sandy sainfoin - 472.1; the third year - sandy sainfoin - 484.0 and alfalfa - 510.3; the fourth year - sandy sainfoin - 489.3. When using perennial legumes as precursors of winter crops, it is necessary to use those species that leave behind the largest reserve of moisture in the soil: in the second year of growing season - white sweet clover - 57.6 mm in a 30-cm soil layer; the third year - sandy sainfoin - 81.1 mm; the fourth year - sandy sainfoin - 88.7 mm in a 30-cm soil layer and eastern goat's rue - 84.8 mm.

Influence of the orientation of uterine bulbs during planting on the seed yield of winter onion variety Ellan in a two-yield direct crop

The article presents the results on the use of paracuneal bulbs for re-harvesting seeds in a direct crop. The influence of the orientation of uterine bulbs during planting on the formation of paracuneal bulbs and seed productivity in the second year of vegetation was determined. The paracuneal bulbs were developing at the base of the flower shoots maintaining the same orientation as in the uterine bulbs when planted. Winter hardiness of paracuneal bulbs depended on orientation, just as with standard technology. Losses during the winter period ranged from 48% to 82%, increasing with an increase in the angle of deviation from the vertical position. The most vulnerable bulbs were the upside down. Paracuneal bulbs can be used to re-harvest seeds in a two-yield crop.

VARIETY RESOURCES OF ALFALFA IN THE INTENSIFICATION OF FIELD FEED PRODUCTION

In the soil and climatic conditions of the Right Bank Forest-Steppe, regardless of the action of abiotic and biotic factors during the growing season, high adaptability of alfalfa was established for different geographical origins, namely the natural zone of the Forest-Steppe, Polissya, Steppe Ukraine and Serbia. It was found that under the weather conditions of 2016 year alfalfa varieties reacted differently to changes in temperature and moisture during the growing season. It is important to note the effectiveness of phosphorus-potassium fertilizers applied directly to alfalfa coverless method of sowing. In the year of sowing, alfalfa formed a powerful herbage and provided two slopes with a yield of green mass at the level of 20,13-29,80 t/ha with a dry matter yield of 4,80-6,69 t/ha. For the second year of vegetation, alfalfa was carried out according to the regimes, which considered the phases of growth and development (budding, beginning of flowering and flowering). In the first mode of cutting management (all slopes in the budding phase) it is advisable to identify three varieties of Unitro, Nasoloda and Rosana, which provided a gross dry matter of 10,41-10,48 and 2,29-2,31 t/ha of crude protein. The productivity of alfalfa increased with late term cutting - at the beginning of flowering and a combination of slopes (3rd mode). The dry matter yield was 11,40-12,62 and 2,35-2,51 t/ha of crude protein (second regime) and the indicators increased under the third regime, especially dry matter 13,58-15,80 t/ha and decreased to 2,37-2,41 t/ha of crude protein. The increase in dry matter of the second mode increased by 9,5-23,1% and crude protein by 2,6-20,5%, and in the third mode of use of alfalfa it was 2,3-11,2%, compared to the mode of mowing the grass in the budding phase. During the third year of vegetation under favorable agroecological conditions for growth and development of alfalfa plants, the studied varieties provided four slopes regardless of the mode of use of grass with a dry matter yield of 15,43-20,58 and 3,21-4,02 t/ha of crude protein. During the second and third years of vegetation from the studied modes of use of alfalfa grass for fodder purposes, the highest yield of crude protein 5,27-6,53 t/ha varieties provided for the alienation of crops at the beginning of the flowering phase, which was on average 6,0-7,1% higher than when mowing all slopes in the budding phase and a combination of slopes in the phases of growth and development (3rd mode). Thus, in the conditions of the Forest-Steppe of the right bank on gray forest soils alfalfa, regardless of geographical origin, adapted to growing conditions and provided stable productivity indicators regardless of the regime of grass use with observance of elements of cultivation technology.

Features of the growth and development of <i>Lycopus americanus</i> Muhlenberg. in the Moscow region

One of the main tasks of botanical gardens is to create and maintain biological collections. There are 5 types of Lycopus in the biological collection of «All-Russian research Institute of medicinal and aromatic plants». One of them is the Lycopus americanus Muhlenberg ex W. P. C. Bart., was the object of this work, as the most difficult in cultivation. In nursery 1-4 years of vegetation researches of growth and development of a Lycopus americanus were carried out. Seeds of Lycopus americanus were received by the exchange Fund from Canada. Sowing was carried out in May 2016 inthe conditions of protected soil in pallets 7×7 cm. Seedlings were grown for two months at a temperature of +18+20 °С and illumination of 1000-1200 lux. In the first decade of July, the nursery was laid with 60-day seedlings according to the scheme 20×60 cm. The area of the plot is 0.8 m2, the repetition is fourfold. Accounting of indicators and processing of results were carried out according to standard methods. Experience in vegetative propagation of Lycopus americanus (2018-2019) got them out from the mother plant and according to the scheme experience, after dividing and planting these divided roots once watered with a solution of homeopathic preparation "Arnica C200" in a concentration of one granule/5 l of water, a flow rate of 5 l/ m2(experiment 1). Of experiment 2, plants after transplantation were once watered with a solution of lactose (milk sugar), the granules of which are filler for homeopathic preparations. The concentration of the solution in option 2 is 1 granule /5 lof water. Control plants were watered with water in the same volume. In the course of the studies it was revealed that the most significant increase in height have plants of the 2nd year of vegetation. The growth decreases with age and in 4-year-old plants reaches minimum. During the season, the greatest increase in height occurs in July and August (in the stalk stage). Watering with a solution of the homeopathic drug "Arnica C200" (experiment 1) during vegetative reproduction of Lycopus americanus causes an increase in height in the first year of vegetation (from 60 days from transplantation) by 30% - 36%. The number of axillary shoots in treated plants by the end of the growing season exceeded the control by 1.6-1.8 times. The dry land mass of treated plants (experiment 1) increased by 34% relative to control group. The treated plants showed an increase in the size of tubers with buds of renewal relative to the control group. The dry mass of the underground part of the treated plants of Lycopus americanus (experiment 1) us by the end of the growing season exceeded that of the control plants by 21%. The average dry mass of 1 tuber at the end of the growing season was 0.63±0.061 gin control plants and 1.17±0.112 gin experimental samples. It is possible to grow Lycopus americanus as a long-term culture in theMoscowregion. The largest increase in biomass of the grain falls on the second year of vegetation. Starting from the third year of vegetation, it is advisable to propagate the plant by dividing the rhizomes in the spring. The homeopathic preparation "Arnica C200" as growth regulator well proved at vegetative reproduction of a Lycopus americanus . Its use causes excessive buildup of Lycopus americanus - storing tubers with the buds of renewal

Насіннєва продуктивність люцерни посівної залежно від елементів технології вирощування

Alfalfa is one of the most productive legumes that provides high protein production. However, it is not only an important source of protein and essential amino acids, but also enriches the soil with biological nitrogen, which increases the fertility of arable land. Lucerne occupies a leading position among legumes in terms of symbiotic activity and fixed atmospheric nitrogen volume. The need to expand areas under perennial grasses in Ukraine is urgent so that, ultimately, the soil is not depleted of the mineral nutrients. Therefore, one of the topical and priority areas in the development of technologies for growing perennial legumes is to improve productivity by studying the processes of growth and development of varieties in the face of climate change and the action of organized factors, which will significantly affect the formation of seed yield and leafy mass. According to the results of our own researches and the analyzed literature sources, it is established that the use of pre-sowing treatment of seeds and crops of alfalfa with sowing stimulants and microfertilizers improves the conditions of growth and development of culture and improves individual productivity. The research was conducted at the research field of Vinnytsia National Agrarian University, Agronomichne, Vinnytsia region during 2016-2018. Sinyuha variety (2010) was sown. Fields (conducting phenological observations and records), laboratory (accounting of seed productivity and its structure), mathematical and statistical (objective evaluation of the results of research). The purpose of the study was to investigate the effect of pre-sowing seed treatment, time and combination of the introduction of growth promoters and microfertilizers on the formation of seed productivity of alfalfa sowing. Agrotechnological techniques for growing alfalfa crops under sowing have been improved through an optimized system of seed treatment with growth stimulants for crop fertilizers with a complete set of macro and microelements. The full-fledged crop of alfalfa sowing seeds begin to form from the second year of vegetation. Observing the level of seed yield of alfalfa seeds, depending on the sowing treatment with growth promoters and microfertilizers, showed that 0.41 t / ha of seed was harvested without treatment. The treatment of sowing with the growth stimulant Saprohum in the branching phase caused the seed yield to increase by 0.02 t / ha and yield 0.43 t / ha. Variants with sowing treatment of growth promoter Saprogum in the branching phase, in the budding phase, dual use of the growth stimulator in the branching and budding phase and in the processing of microfertilizer sowing Vuxal in the budding phase provided a yield increase of 0.03 t / ha to 0.44 t / ha Ha. The highest seed yield was ensured by the Saprohum growth and saplings treatment + branching + sowing fertilizer Vuxal - 0.46 t / ha, which is 0.05 t / ha more than the variant without the introduction of growth and microfertilizers. . Observation of seed weight of alfalfa from one plant when using a combination of a growth promoter Lucis and microfertilizers «Urozhay bobovi» showed that the treatment of sowing with a stimulator Lucis in the branching phase contributes to the increase of seed weight by 5.3% - up to 0.94 g Vegetable vegetation four years. The highest seed yield from one plant was recorded on the variant of sowing treatment with the growth stimulator Saprogum in the branching and budding phase + fertilizing of the budding phase by the microfertilizer Vuxal - 1.0 g, which is 11.0% more than the variant without sowing with stimulants and microfertilizers. Comparison of the magnitude of the seed output from one alfalfa plant under different combinations of growth stimulants and microfertilizers showed that the combination of sowing with a growth stimulants Lucis in the branching and budding phase + fertilizing the crop in the budding phase with microfertilizers of sowing treatment with a growth stimulant Saprohum in the branching and budding phase + fertilizing of the budding phase by the microfertilizer phase Vuxal. The highest yields of alfalfa seeds were obtained by sowing variants with the Lucis growth stimulants and microfertilizers «Urozhay bobovi» was 0.48 t / ha. A close correlation-regression relationship was established between the yield of alfalfa seeds and: plant density, plant height and individual seed productivity of one plant. The expected results of the research are aimed at increasing the level of realization of the crop varietal potential of perennial grasses, namely, alfalfa, increasing the profitability of its production in combination with environmental and social effects.

Effect of plant’s alimentation area on productivity of Oregano plants (Origanum vulgare L.) under conditions of a drip irrigation

Oregano (Origanum vulgare L.) – is a perennial medicinal plant, its medicinal raw material is the aerial part collected in the flowering phase. When laying industrial plantations of oregano, the seedling method of propagation is mainly used, since after germination the plants slowly develop and compete weakly with weeds. The condition for survival of seedlings is high soil moisture, which can be achieved only with its artificial wetting - irrigation. An analysis of the literature indicates that today the issue of the cultivation pattern and nutrition area of oregano in the conditions of drip irrigation is not well understood. The aim of the conducted experimental studies was to establish the influence of the method of growing seedlings and plant’s alimentationon area on the growth and development of oregano under drip irrigation.
 The main research method is a field experiment, supplemented by analytical and static data processing methods. According to research’s results, it has been established that an increase in the area of plant nutrition contributes to an increase in the mass of the aerial parts and the area of leaves, and also reduces the height of plants during both the first and second years of vegetation. It was proved that the most favorable conditions for plant’s growth and development under the conditions of drip irrigation were in the variant with the largest nutrition area with planting density of 41,7 thousand plants·ha-1 (60x40 cm pattern).
 The mass of the aerial parts of plants with this method of growing in the first year of vegetation was 110,5-133,0 g ·plant-1 with the largest leaf area – 0,287-0,346 m2·plant-1 and 218,1-328,7 g ·plant-1, 0,568-0,855 m2·plant-1, respectively - in the second year of vegetation.
 The maximum height of plants – 37,0-37,7 cm in the first year of plant vegetation and 68,5-72,6 cm in the second year of vegetation was established in the variants with the smallest plant nutrition area: with a growing scheme of 60x10 cm (166,7 thousand ·ha-1). The smallest plant’s height in the first year of vegetation was 31,1-33,5 cm, in the second year – 37,5-48,4 cm in the variant of growing scheme 60x40 cm (41,7 thousand plants ·ha-1). When studying the influence of the method of growing seedlings on the mass of the aerial part and the area of leaves, their high parameters were found in variants with the laying of seedlings from spring sowing cassettes, where the mass of the aerial part was 103,8 g / plant with a leaf area of 0,236 m2 ·plant-1. The minimum weight of the aerial part is 92,3 g·plant-1 with a leaf area of 0,210 m2 ·plant-1 was found in variants with seedlings on cassettes from the autumn sowing period.

ECONOMIC EFFICIENCY OF TECHNOLOGICAL METHODS OF GROWING OF SWITCHGRASS

The article presents economic assessment of technological methods of cultivation of switchgrass. The most effective technologic activities to ensure maximum growth yield of dry biomass of switchgrass and the highest econonomic efficiency. The highest efficiency was obtained at the variant where there have been two pre-cultivation before and after the rolling ladder. The varieties Сave-in-rock and Сarthage the third and fourth years the farming cost of production from the realization of biomass in this variant amounted to 11,875 and 15,390; 9,595 and 13,870 UAH/ha, and profit from sales of products – 4,157 and 7,381; 1,974 and 5,950 UAH/ha and profitability of Cave-in-rock – 92,2 and Сarthage – 75.1 per cent in the fourth year of vegetation. A high level of profitability obtained on the variant, where conducted, only two pre-cultivation the varieties of millet lozovenko the fourth year of vegetation: Cave-in-rock – of 87.0 and Сarthage was 69.2%. The cultivation of plants during second year of vegetation was not effective, the level of profitability at grades Сarthage from 25,6 to -43,3% and cave-in-rock from -12,6 to -36,7%. The highest indicators of economic efficiency were obtained in the variant with row-spacing width of 45 cm from plant switchgrass third and fourth year of cultivation. The varieties Cave-in-rock and Сarthage the cost from the sale of biomass amounted to 12635 and 15675; 7885 and 13870 UAH/ha, and profit from sales of products – 4844 and 7553; 493 and 5950 UAH/ha profitability of millet varieties depended primarily on year of vegetation, width of row spacing and varietal characteristics. The highest level of profitability obtained from varieties of millet the fourth year of vegetation with a width between rows 45 cm – 75.1% in the variety Cartage and 93.0% of the varieties cave-in-rock. A lower level of profitability obtained for the variant in terms of 2017, where inter-row spacing was 30 cm from millet varieties cave-in-rock – 73,6% and Cartage – 50.5 per cent. Key words: switchgrass, row spacing, pre-sowing soil.

Influence of methods for growing seedling and food area on the formation of vegetative mass of medicinal melissa (Мelissa officinalis L.) under the drop irrigation

Studies have been carried out to identify the influence of the nutritional area and methods of growing seedlings on the formation of the vegetative mass of the medicinal product under drip irrigation. A direct dependence of the weight of the formed phytomass of the aerial part and the area of the leaf apparatus from the plant nutrition area is revealed — an increase in the nutrition area contributes to an increase in the weight of the aerial part and the leaf area. For the sake of law, it is possible to stand up, like on the first one, so on the other side of the vegetative melissa.It has been established that more favorable conditions for the growth and development of medicinal potassium are created with a growing scheme of 60×40 cm with a planting density of 41.7 thousand seedlings. plant./ha, where the weight of the aerial parts of plants of the second year of vegetation varied between 332.5–582.4 g/plant., and the leaf area — 0.825–1.446 m2 /plant. respectively.When determining the influence of the method of growing medicinal lemon balm seedlings on the processes of forming the height and vegetative mass, it was revealed that the indicators of the options laid by the seedlings grown in winter sowing cassettes were higher for the weight of the aerial part and the assimilating leaf area. The variants have planted a ridge from the ridge, the sprawls behind the biometric indicators of the boules are the most middle-level varieties

Features of energy willow biomass accumulation on floodplain soils of Polissia (Ukraine)

Purpose. To determine the parameters of the main factors affecting the productivity of energy plantations of willow on the floodplain soils of Polissya (Ukraine).Methods. The experimet with energy willow plantations were created using annual cuttings of the varieties ‘Zbruch’, ‘Ternopilska’ and a Salix triandra willow clone, planted in double rows with a distance of 0.75 m between them and with a row spacing of 1.5 m. Planting cuttings density: 10, 15 and 20 thousand pcs/ha.Results. After the first growing season in the 'Ternopilska' variety, the survival rate of cuttings on peat-bog soil ranged from 48.6 to 54.8 %, in the 'Zbruch' variety from 72.8 to 86.6 %, and in Salix triandra from 71.0 to 82.3 %. During the second growing season there was a significant decline in plants, especially in denser stands. On sandy soil, after the second year of vegetation, from 74.1 to 87.5 % of plants remained. Biomass productivity in the first 3 years on peat-bog soil was 0.58–1.10 t/ha/year in ‘Ternopilska’ variety, 0.80 to 3.28 t/ha/year in ‘Zbruch’ variety, and from 0.62 to 0.96 t/ha/year. At the same time, the density of plantations and edaphic conditions significantly affected the productivity of the dry mass of plants. In particular, on sandy loam soil, 'Ternopilska' variety in the first 2 years had productivity from 0.77 to 3.21 t/ha/year, which is significantly higher than on rich of organic substainces moistened peat-bog soils. The largest leaf area was in 'Ternopilska' variety on sandy loam soil, from 22.0 to 31.3 thousand m2/ha, and in 'Zbruch' variety (from 17.2 to 27.5 thousand m2/ha). The average photosynthetic potential according to the experimental options ranged from 1.67 to 5.06 million m2·day/ha, increasing with increasing planting density and leaf area. The highest net productivity of photosynthesis indicators are observed in 'Ternopilska' variety on sandy soil, from 0.98 to 1.62 g/m2 per day and in ‘Zbruch’ variety (0.70–1.16 g/m2 per day). At the same time, these indicators were maximum with a density of 15 thousand plants./ha.Conclusions. In floodplains of the Polissia, sandy loamy soils are more suitable for growing the willow energy biomass compared to peat-bog soils. Willow energy plantations have a large leaf surface area, high photosynthetic potential and net photosynthesis productivity, which provides relatively high biomass productivity indicators. On peat-bog soils from the studied varieties, it is advisable to use ‘Zbruch’ variety, which is characterized by high biomass productivity (3.28 t/ha/year), to create willow energy plantations.

VARIABILITY OF THE MAIN AGRONOMIC VALUABLE TRAITS OF MOUNTAIN SAVORY (<i>SATUREJA MONTANA</i> L.) IN «BOBRIK» VARIETIES

In recent years, interest in mountain savory is increasing and this is due to the rich biochemical composition, useful properties and decorative qualities of this plant. The materials were sortopopulyatsiya savory mountain. The study was conducted in the open ground in Moscow region (2012-2018) on experimental plots of the laboratory green, spicy taste and floral crops of FSBSI FSVC. The work on improving the source material by applying individual selection, the most aligned with economically valuable traits form ЧГ32-12 with high potential productivity of inflorescences and green mass, decorative, flavour and hardiness. Conducted testing and pre-multiplication of promising accessions, which was transferred into the "GOSSORTKOMISSIA" in 2018 called “Bobrik”. The yield of green increased in vegetation years in the first year of vegetation was 0,650 kg/m2, in the second – 3.0 kg/m2, and in the third year reached 5.4 kg/m2. Indicator sign "plant height" increased over the years of vegetation (11.4 cm, 25.5 cm and 40.2 cm, respectively). The values of the sign "number of shoots" in the first year of vegetation was 11.6 PCs., in the second year – sharply increased (255.1 PCs.), in the third year – reached its maximum (787.3). The accumulation of dry matter in the years of vegetation increased: the first year contained 20.08%; the second year – 26.95%; and the third year of vegetation – 29.71%. The content of ascorbic acid in the flowering phase in the second year of vegetation increased compared to the first year (8.21 mg/% and 5.87 mg (%). The amount of water-soluble antioxidants in the second year of the growing season has increased almost two times in comparison with the first year (to 22.7 mg/g and AAE 42.39 mg/g of AAE), in the third year there is a decrease in rate – 28.6 mg/g of AAE. Thus, the green of the mountain variety "Bobrik" is a source of biologically active substances, such as ascorbic acid and carotenoids, so the culture can be recommended for the use of young shoots and leaves in fresh and dry form, as a seasoning for salads, sauces, marinades, meat and fish dishes, as well as spicy and aromatic additives for the preparation of drinks.

Potential phytomanagement of military polluted sites and biomass production using biofuel crop miscanthus x giganteus

This study aims to summarize results on potential phytomanagement of two metal(loid)-polluted military soils using Miscanthus x giganteus. Such an option was tested during 2-year pot experiments with soils taken from former military sites in Sliač, Slovakia and Kamenetz-Podilsky, Ukraine. The following elements were considered: As, Cu, Fe, Mn, Pb, Sr, Ti, Zn and Zr. M. x giganteus showed good growth at both military soils with slightly higher maximum shoot lengths in the second year of vegetation. Based on Principal Component Analysis similarities of metal(loid) uptake by roots, stems and leaves were summarized. Major part of the elements remained in M. x giganteus roots and rather limited amounts moved to the aerial parts. Levels taken up decreased in the second vegetation year. Dynamics of foliar metal(loid) concentrations divided the elements in two groups: essential elements required for metabolism (Fe, Mn, Cu, and Zn) and non-essential elements without any known metabolic need (As, Sr, Ti, and Zr). Fe, Mn, Ti and Sr showed similar S-shaped uptake curve in terms of foliar concentrations (likely due to dilution in growing biomass), while Cu exhibited a clear peak mid-season. Behavior of Zn was in between. Foliar Zr and As concentrations were below detection limit. The results illustrated a good potential of M. x giganteus for safely growing on metal-polluted soils taken from both military localities.

ЕКОЛОГО-БІОЛОГІЧНІ ОСОБЛИВОСТІ ЛОПУХА ВЕЛИКОГО ARCTIUM LAPPA L. ТА КОТЯЧОЇ М'ЯТИ СПРАВЖНЬОЇ NEPETA CATARIA L. В УМОВАХ ПОЛІССЯ УКРАЇНИ

Наведено результати досліджень із вивчення еколого-біологічних особливостей лопуха великого Arctium lappa L. та котячої м'яти справжньої Nepeta cataria L. в умовах ботанічного розсадника Житомирського національного агроекологічного університету. Мета роботи полягала у вивченні інтродукції, шляхів підвищення біологічної продуктивності досліджуваних рослин в умовах Полісся України. На основі багаторічних досліджень вперше вивчено можливість і способи одночасного вирощування лопуха великого на листок і корінь у підзимніх і ранньовесняних поточних і перехідних посівах другого року вегетації. Під час вирощування Arctium lappa L. на корені запропоновано однорічний термін вирощування культури із збиранням сировини у другій половині вересня. Підрізування головного кореня на глибині 15–20 см у фазі росту другого-третього справжнього листка знижувало біологічну продуктивність кореневої системи на 10–15 %. В умовах Полісся України доцільно вирощувати котячу м'яту справжню як дворічну культуру. Максимальна врожайність повітряно сухої сировини формується у котячої м'яти справжньої у фазі масового цвітіння і залежно від погодних умов у середньому за роками досліджень становить у рослин першого року життя 185,0 г/м2, у рослин другого року життя – 367,0 г/м2. Вміст ефірної олії у повітряно сухій сировині характеризується невисокими показниками. У середньому за роки досліджень у рослин котячої м'яти справжньої першого року життя 0,07 %, другого року життя – 0,14 %.

Creation of Fodder Agrophytocenoses for the Restoration of Degraded Native Pastures and Improvement of their Performance

This article presents the results of two years of development of technological methods to create fodder agrophytocenoses in natural environments under arid climatic conditions in order to restore the ecology and improve the productivity of degraded pasturable ecosystems. The dynamics of the phytopotential performance of agrophytocenoses have been studied in different variants according to plant species, sowing times, and sowing methods. The experiments have been conducted with methods developed by the All-Russia Research Institute of Fodder (2015) and Dospekhov (1985). Therefore, the experiments conducted with the different pasturable plants investigated that the winterfat (Eutoria ceratoides) was reputed to be the most productive of all of the sown species. Its annual average crop yield was three times higher than that for the native pasture in the first year and 3.6 times in the second year. The experiments conducted with wheatgrass (Agropyron) sown at different times have proved that the pasture was generally (71–76%) occupied by wheatgrass (Agropyron) sown in autumn in the first year of vegetation, while the spring-sown wheatgrass crop had a projective cover of only 49–54% up to the second year of vegetation. The experiments conducted with wheatgrass sown by different methods proved that the spring- and autumn-sown projective cover crops of agrophytocenoses with the use of the broadcasting method represented increases of 7% and 17%, respectively, as compared to the drilling method.