Prospects and methods of polyploid selection of new high-yielding miscanthus clones in the natural climatic conditions of Ukraine

Abstract

Goal. Expanding the breeding trait collection of breeding genotypes of the genus Miscanthus (Anderson) and the genetic basis for new allotriploid clones by transferring components for hybridization of natural wild species Miscanthus sinensis and Miscanthus sacchariflorus to the tetraploid level.
 Methods. Cytological, biotechnological, fluorescent cytphotometry, field, laboratory.
 Results. The efficiency of polyploidisation for induction of new tetraploid forms of miscanthus in liquid nutrient media supplemented with colchicine (0.05% mass) and for stabilization of myxoploid shoots (0.005% mass) for 6 h of cultivation is examined. The period of exposure for Miscanthus sinensis (2x=2x= 38) and Miscanthus sacchariflorus (2x = 2x = 38) varied from 2 hours to 3 days depending on the genetic origin of the material, with transfer to a hormonal environment. The best indicators of tetraploid induction for Miscanthus sinensis were observed for the exposure period of 2 days with polyploidization efficiency (Db%) of 31.25% and 21.42%, and for Miscanthus sacchariflorus 2 and 6 hours with 35.0% and 27.3%, respectively. The technology of transfering rooted shoots into the soil on the Yaltushkiv Eperimental Breeding Farm was improved and the composition of the soil mixture that provided 99% rooting of culture seedlings at a humidity of 60−70% and air temperature of 35−55°С was found.
 Conclusions. New biotechnological tetraploid lines of Miscanthus sinensis (2xn=4x=76) and Miscanthus sacchariflorus (2xn=4x=76) were created in the conditions of liquid nutrient media supplemented with colchicine (0.05 % mass). It is investigated that flowering of new tetraploid clones in the conditions of Ukraine for the second year of vegetation occurs from late September to early October with formation of fertile pollen grains.