The study purpose to examine the effect of using think-pair-share cooperative learning strategy on nursing students' perception of their learning. A quasi-experimental pre- and post-test design was used to examine if think- pair-share cooperative teaching strategy would improve learning perception measured by learning scale. This study took place with a sample of 90 student nurses enrolled in third year and taking the scholarly inquiry course in the second semester of academic year 2018-2019. According to the study results, although there are no significant results in the comparison with pre and posttest mean scores of learning perception scale, the findings indicate high statistically significant difference and positive moderate relationship among all categories of learning perception scale and Cooperative Learning Attitude. Moreover, the study participants have statistically significant different with high level of learning perceptions for all Learning Perceptions Categories Namely Integration of Learning, Understanding Content, and Increase in Skills. In conclusion, this study supports continued use of cooperative learning approach. Also, results pointed to the importance role of cooperative learning in nursing education and the need for further teaching and learning strategies which needs students to utilize teamwork to assist everyone for improving their learning and learning of others.