In this study, to improve the vibration isolation performance of a cab seat and the ride comfort of the driver, we propose a mathematical model for a seat suspension system of a construction machinery cab based on a negative stiffness structure (NSS). First, a static analysis of a seat suspension system is conducted and the different parameters and their influences on the dynamic stiffness are discussed. Thereby, the ideal configuration parameter range of the suspension system is obtained. Moreover, the nonlinear dynamic model of the designed seat suspension system is established. The frequency response and the stability are analyzed by using the HBM method and numerical simulation. The vibration transmissibility characteristics and vibration suppression effects of the seat suspension system are presented in detail. The results show that, as compared with a quasi-zero-stiffness system, the QZS-IE system has higher vibration suppression advantages under large excitation and small damping, as well as lower transmissibility and a wider vibration isolation frequency range. In addition, an inerter element with a larger mass ratio and relatively shorter distance ratio is better for vibration isolation performance of the QZS-IE system in a practical engineering application. The results of this study provide a scientific basis for the design and improvement of a seat suspension system.