Seasonal Freeze Thaw Research Articles

Effects of freeze–thaw on the detachment capacity of soils with different textures on the Loess Plateau, China

Soil detachment capacity (Dc) is a crucial indicator for quantifying erosion intensity. However, the combined actions of freeze–thaw and water flow complicate the erosion process, leaving the variation mechanism of Dc under this condition systematically unexplored. This study examined five loess soils from a seasonal freeze–thaw area. The mechanism driving changes in Dc was quantified through freeze–thaw simulation combined with flow scouring tests, and a Dc prediction model was established. The results revealed that the shear strength (τm), cohesion (Coh), and internal friction angle (φ) in silt loam were higher than in sandy loam. After freeze–thaw cycles (FTC), τm, Coh, and φ of the five loess soils decreased by 1.02–1.37, 1.07–9.15, and 0.92–1.05 times, respectively. As FTC increased, τm and Coh gradually stabilized, while φ showed minimal fluctuation, indicating that FTC had a cumulative effect on the deterioration of soil mechanical properties. During FTC, Dc in Wuzhong sandy loam was the largest, being 1.14–3.24 times greater than in other soils, suggesting a significant main effect of soil type on Dc variation, with a contribution rate of 19.27 %. Dc eventually stabilized with increasing, indicating a critical FTC of around 10 for its impact on Dc. Compared to unfrozen soils, Dc increased by 33.69 %–102.40 % under the combined effects of freeze–thaw and water flow, clarifying that FTC aggravated soil instability. Effective stream power was the optimum hydraulic parameter, contributing the most to Dc (45.94 %). FTC (6.41 %) and initial soil moisture content (8.59 %) were less influential, as FTC initially degraded soil properties, and then the combined action with water flow intensified soil damage, causing the role of freeze–thaw factors to be obscured by other variables. A Dc prediction model using a general flow intensity index estimated well Dc, with both R2 and NSE at 0.94. Model performance comparison emphasized the need for validation when extending the application range beyond development conditions. These findings provide new insights into the detachment mechanisms of different textured soils under compound freeze–thaw and hydrodynamic influence in freeze–thaw region.

Correction: Metal(loid)s Removal Response to the Seasonal Freeze–Thaw Cycle in Semi-passive Pilot Scale Bioreactors

Correction: Metal(loid)s Removal Response to the Seasonal Freeze–Thaw Cycle in Semi-passive Pilot Scale Bioreactors

Chestnut soil organic carbon is regulated through pore morphology and micropores during the seasonal freeze–thaw process

Soil aggregates are basic structural units in soil organic carbon (SOC) protection. In alpine ecosystems, the seasonal freeze–thaw (FT) process characterizes soil formation and nutrient cycling. However, previous studies were mostly based on simulated FT experiments, which amplified the effects of natural FT processes. And, the regulations of pore structure on SOC protection/loss of aggregates during the FT processes were still not well understood. To investigate the effect of the seasonal FT process on SOC and pore structure of aggregates, as well as the interactions among them, soil samples were selected during a whole seasonal FT cycle, which can be divided into four periods: unstable freezing (UFP), stable frozen (SFP), unstable thawing (UTP), and stable thawed periods (STP). The results demonstrated that freezing increased SOC concentration as the total organic carbon (TOC) content of all aggregate fractions peaked in the SFP (17.46 g/kg on average). The TOC content of aggregates in the UFP dropped to 7.91 g/kg on average, which revealed a dramatic SOC loss after thawing began. Thawing also decreased the proportions of particulate organic carbon (POC) compared with mineral-associated organic carbon (MAOC). The highest microbial abundance was also found in the SFP. Freezing promoted the formation of pores > 80 μm while thawing increased the regularity of pore morphology. Pore structure explained 48.77 % of the SOC variance in the thawing period, but only 19.29 % of that in the freezing period. Overall, in the freezing process, soil pore structure impacted the SOC input by mediating pore morphology. In the thawing process, soil pore structure inhibited SOC loss by enhancing the formation of pores < 15 μm. These results demonstrate new perspectives on the soil aggregate microstructure–microbe–SOC interactions.

Metal(loid)s Removal Response to the Seasonal Freeze–Thaw Cycle in Semi-passive Pilot Scale Bioreactors

There is an increasing demand for cost-effective semi-passive water treatment that can withstand challenging climatic conditions and effectively and sustainably manage mine-impacted water in (sub)arctic regions. This study investigated the ability of four pilot-scale bioreactors inoculated with locally sourced bacteria and affected by a freeze–thaw cycle to remove selenium and antimony. The bioreactors were operated at a Canadian (sub)arctic mine for a year. Two duplicate bioreactors were installed in a heated shed that was maintained at 5 °C over the winter, while two other duplicates were installed outdoors and left to freeze. The removal rate of selenium and antimony was monitored weekly, while a genomic characterization of the microbial populations in the bioreactors was performed monthly. The overall percentage of selenium and antimony removal was similar in the outside (10–93% Se, 20–96% Sb) and inside (35–94% Se, 10–95% Sb) bioreactors, apart from the spring thawing period when removal in the outdoor bioreactors was slightly lower for Se. The dominant taxonomic groups of microbial populations in all bioreactors were Bacteroidota, Firmicutes, Desulfobacterota and Proteobacteria. The microbial population composition was consistent and re-established quickly after spring thaw in the outside bioreactors. This demonstrated that the removal capacity of bioreactors inoculated with locally sourced bacteria was mostly unaffected by a freeze–thaw cycle, highlighting the strength of using local resources to design bioreactors in extreme climatic conditions.

Effects of freeze-thaw leaching on physicochemical properties and cadmium transformation in cadmium contaminated soil

This study aims to investigate the effect of the combined method of freeze–thaw and leaching on the removal of cadmium (Cd) in soil and to provide a theoretical basis for the remediation of farmland soil polluted by heavy metals. The removal process and mechanism of Cd were deduced through oscillatory leaching experiments and freeze–thaw leaching simulation experiments, and the influence of the freeze–thaw leaching technology on the soil environment was evaluated. The results of oscillatory leaching showed that a mixture consisting of 0.80 mol/L citric acid and 0.80 mol/L ferric chloride in a 1:19 vol ratio effectively remove 47.75 % of Cd, indicating that the composite leaching agent could effectively remove Cd from the soil. The results of the freeze–thaw leaching simulation experiment showed that although the freeze–thaw leaching treatment increased the total Cd content in the 0–5 cm soil layer, the total Cd content in the 5–10 cm, 10–15 cm, and 15–20 cm soil layers decreased by 5.08 %, 2.39 %, and 5.68 %, respectively. The freeze–thaw leaching increased the content of exchangeable Cd (p<0.05), carbonate bound Cd, but decreased organic bound Cd and residual Cd (p<0.05), thereby increasing the bioavailability of Cd. Freeze–thaw leaching not only increased the competitive adsorption of Cd2+ by decreasing soil pH, cation exchange capacity, and increasing the content of exchangeable calcium and exchangeable magnesium, thus reducing the adsorption of Cd in soil. And the results of XPS and FTIR similarly showed that the freeze–thaw leaching could promote the chelation between Cd2+ and hydroxyl, carboxyl and carbonyl functional groups. Although the freeze–thaw leaching destroyed the large particle structure (0.05–2 mm) and large pores in the soil, and increased the clay content (<0.002 mm) and the proportion of small pores in the soil, the XRD results showed that freeze–thaw leaching had no significant effect on the minerals in the soil. In summary, this study shows that freeze–thaw leaching has a significant effect on the removal of soil heavy metals, suggesting that the synergistic effect of freeze-thaw and leaching should be considered in the process of removing soil pollutants in seasonal freeze–thaw zones, and that this method provides a new insight into the remediation of contaminated soils.

Mechanisms of Suprapermafrost Groundwater Recharge Streamflow in Alpine Permafrost Regions: Insights From Young Water Fraction Analysis

AbstractThis study investigates the temporal processes of suprapermafrost groundwater (SPG)‐supplied streamflow in alpine permafrost regions, aiming to fill the gap in understanding this process from a water‐age perspective. Precipitation, streamflow, and SPG samples were collected from the Three‐Rivers Headwaters Region (TRHR). We defined the physical meaning of Fyw (the young water fraction) of the SPG and calculated it for the first time. The results showed that in the TRHR, the SPG mean travel time (MTT) was 159 days, and approximately 46.4% of SPG was younger than 77 days, whereas the streamflow MTT was 342 days, and approximately 12.2% of the streamflow was younger than 97 days. The correlation analysis revealed that various climatic factors played dominant roles in the recharge time variations of the SPG‐supplied streamflow within the TRHR. The SPG recharge rate did not significantly affect the streamflow Fyw; however, the thickness of the active layer ultimately controlled the SPG transit time distribution. Regression analysis further demonstrated the nonlinear impact of precipitation, average temperature, and average freezing days on SPG Fyw, which is closely related to seasonal freeze–thaw heat conduction and groundwater heat advection in the active layer. During the initial ablation period, the streamflow was primarily recharged by young SPG, resulting in a short‐tail travel time distribution. Our findings provide valuable insights into runoff generation and concentration processes in permafrost regions and have important implications for water resource management.

Seasonal freeze‒thaw processes impact microbial communities of soil aggregates associated with soil pores on the Qinghai–Tibet Plateau

BackgroundSeasonal freeze‒thaw (FT) processes alter soil formation and cause changes in soil microbial communities, which regulate the decomposition of organic matter in alpine ecosystems. Soil aggregates are basic structural units and play a critical role in microbial habitation. However, the impact of seasonal FT processes on the distribution of microbial communities associated with soil pores in different aggregate fractions under climate change has been overlooked. In this study, we sampled soil aggregates from two typical alpine ecosystems (alpine meadow and alpine shrubland) during the seasonal FT processes (UFP: unstable freezing period, SFP: stable frozen period, UTP: unstable thawing period and STP: stable thawed period). The phospholipid fatty acid (PLFA) method was used to determine the biomass of living microbes in different aggregate fractions.ResultsThe microbial biomass of 0.25–2 mm and 0.053–0.25 mm aggregates did not change significantly during the seasonal FT process while the microbial biomass of > 2 mm aggregates presented a significant difference between the STP and UTP. Bacterial communities dominated the microbes in aggregates, accounting for over 80% of the total PLFAs. The microbial communities of soil aggregates in the surface layer were more sensitive to the seasonal FT process than those in other soil layers. In the thawing period, Gram positive bacteria (GP) was more dominant. In the freezing period, the ratio of Gram-positive to Gram-negative bacterial PLFAs (GP/GN) was low because the enrichment of plant litter facilitated the formation of organic matter. In the freezing process, pores of 30–80 μm (mesopores) favored the habitation of fungal and actinobacterial communities while total PLFAs and bacterial PLFAs were negatively correlated with mesopores in the thawing process.ConclusionsThe freezing process caused a greater variability in microbial biomass of different aggregate fractions. The thawing process increased the differences in microbial biomass among soil horizons. Mesopores of aggregates supported the habitation of actinobacterial and fungal communities while they were not conducive to bacterial growth. These findings provide a further comprehension of biodiversity and accurate estimation of global carbon cycle.

Microbially-mediated reductive dissolution of Fe-bearing minerals during freeze-thaw cycles

Constraining the role of microbes in the structural iron (Fe) reduction of iron-bearing minerals improves our understanding of sediments and ice sheets as a source of dissolved Fe (dFe) to the oceans. However, bio-mediated structural Fe-reduction has yet to be studied in cryospheric environments. Here, we show that the Fe reducing psychrophile bacterium Shewanella vesiculosa, isolated from sea ice in Antarctica, reduced structural Fe in nontronite (NAu-2) and maghemite (γ-Fe2O3), common mineral phases in glacial ice, and marine sediments in Antarctica, during two freeze–thaw cycles (−10 °C to +15 °C), resulting in the release of dFe. The modification of turbostratically disordered nontronite (ferric iron dominant phase) to discrete ordered illite-like structure (ferrous iron dominant phase), and the aggregation of altered small maghemite particles with neoformation of vivianite (Fe3(PO4)2·nH2O) indicated the microbially induced reductive dissolution of nontronite and maghemite, respectively. The biotic Fe-reduction gradually decreased and ceased as the temperature approached freezing (−8 °C), however the rection reactivated in the thawing cycle (−7 to +15 °C). No discernable biotic Fe-reduction was measured for either mineral under freezing conditions, suggesting that temperature limits the activity of the microbes. Further, and regardless of temperatures during two freeze–thaw cycles, Fe-reduction was not observed in abiotic control. Overall, these results highlight the importance of microbially induced Fe reduction during seasonal freeze–thaw cycles of ice and sediments in continuous supplying bioavailable dFe to cryospheric environments and the often Fe-limited polar oceans.

Divergent controlling factors of freeze–thaw-induced changes in dissolved organic carbon and microbial biomass carbon between topsoil and subsoil of cold alpine grasslands

Freeze-thaw (FT) processes in cold regions significantly influence the mineralization and sequestration of soil organic carbon (SOC), particularly in its active pools, such as dissolved organic carbon (DOC) and microbial biomass carbon (MBC). However, manipulation experiments often amplify the effects of natural FT processes and overlook soils below 20 cm depth. To investigate carbon responses to seasonal FT events across soil depths (0–80 cm) and identify controlling factors, field sampling was conducted before freezing and after thawing in alpine grassland soils on the Qinghai-Tibet Plateau. Results are as follows: 1) After soil thawing, the entire soil profile showed insignificant changes in SOC (−4.46%) and MBC (3.21%), but DOC (45.27%) and DOC/SOC (160.08%) increased significantly, and strong associations were observed in DOC changes between adjacent soil layers. 2) FT indicators (number and temperature amplitude of daily FT cycles, frozen temperature, and frozen days) influenced SOC changes differently in the topsoil (0–10 cm) and subsoil (20–30 cm). Contrary to the impact of frozen temperature, other FT indicators largely facilitated the production of DOC0–10 and DOC20–30. The effects of FT indicators on MBC were nonlinear. 3) Structural equation models indicated that average soil water governed FT-induced changes in both DOC0–10 and DOC20–30. The varying effects of porosity and pH from topsoil to subsoil, along with the weakening relationship between changes in DOC and MBC, favored a stronger increase in DOC20–30. Increases in soil water and DOC after thawing stimulated the increment of MBC0–10, while porosity (positively) and sand content (negatively) regulated the MBC20–30 change. It indicates that, influenced by soil structure and biogeochemical environment, DOC and MBC in grassland soils are more sensitive to natural FT processes than SOC. Nevertheless, changes in FT patterns due to long-term climate change may cumulatively affect SOC.

Study on the formation mechanism and preventive measure of pot cover effect for subgrade in seasonal frozen soil area under freeze–thaw cycles

AbstractThe presence of an impervious cover layer inhibits the free evaporation of moisture in the soil during seasonal freeze–thaw cycles, leading to a phenomenon known as the pot cover effect. This can result in severe frost heave issues in airport runways, highway subgrades, railway subgrades, and other similar infrastructure. In this study, a disease investigation was conducted at a gas transmission station situated in an area with seasonally frozen soil. Meanwhile, the causes of moisture accumulation and frost heave in the lower part of concrete pavement slabs were analyzed. A coupled water–vapor–heat model for unsaturated frozen soil is then established to analyze the formation mechanism of the pot cover effect in the subgrade. Finally, the preventive effect of the impervious layer on moisture migration in the pot cover effect was analyzed. The results indicate that the pot cover effect causes a significant accumulation of moisture beneath the concrete pavement at the gas transmission station, resulting in a moisture content increase of 5%–35%. During the freezing period, the vapor of shallow soil migrates upward, while the liquid water remains unchanged. During the melting period, both vapor and liquid water migrate downward. The laying of the impervious layer can prevent moisture migration of the pot cover effect. When the impervious layer is placed on the freezing front (0°C), the prevention effect of the pot cover effect is the best.

Effect of seasonal freeze–thaw process on spatial and temporal distribution of soil water and its infiltration to recharge groundwater

AbstractClarifying the distribution and dynamics of soil moisture during the freeze–thaw process is crucial for surface ecology and is an objective requirement to investigate the mechanism of changes during the groundwater recharge process in a freeze–thaw zone. Based on the monitoring data of soil moisture and temperature in the Changbai Mountain area, the freeze–thaw process is classified into four periods. This study investigates the hydrothermal migration processes during different periods. The simultaneous heat and water model is used to simulate and analyse the infiltration of soil moisture into groundwater under five precipitation insurance rates. The results are as follows: (1) The smaller the soil depth, the stronger is the correlation between soil temperature and air temperature during the freeze–thaw process. (2) The redistribution of soil moisture before and after freeze–thaw is significantly affected by the soil texture, and soil permeability affects the recharge of soil moisture from the upper region to the lower region during the thawing period. (3) Groundwater receives vertical infiltration recharge mainly during non‐freezing and is supplied by freezing and snowmelt recharge during the stable thawing period. The percentage of soil water infiltration during the stable thawing period in the total annual infiltration increases gradually with the precipitation insurance rate.

Compression behavior of CFST columns under combined influence of gap and freeze-thaw

Owing to factors related to construction quality and environment, concrete-filled steel tube (CFST) structures that have been in use for many years in regions experiencing seasonal freeze–thaw cycles are susceptible to the combined effects of a spherical-cap gap and freeze–thaw cycling. Therefore, in this study, a combination of experiments and the finite element method is used to investigate the effects of these two factors on the axial compression behavior of CFST stub columns. The experimental results show that the main failure mode of the specimen is local buckling at the end or the middle and inward depression of the steel tube on the existing gap side. The degradation of mechanical parameters, such as the ultimate bearing capacity and ductility of the specimens, gradually increased with increasing gap ratio and number of freeze–thaw cycles. In the numerical simulation section, the change in the stress response to freeze–thaw cycling on the gap side is analysed. The impact of the constraint effect coefficient on the ultimate bearing capacity degradation of the specimens is also investigated. Finally, a formula for calculating the ultimate capacity of CFST stub columns affected by the two factors is proposed, and the formula for calculating the ultimate bearing capacity in the code is improved. The results of this study can provide a reference for durability prediction and structural safety evaluation of CFST structures with a spherical-cap gap in cold regions.

Combined Effect of Freeze–Thaw Cycles and Biochar Addition on Soil Nitrogen Leaching Characteristics in Seasonally Frozen Farmland in Northeast China

Global climate warming and increased climate variability may increase the number of annual freeze–thaw cycles (FTCs) in temperate zones. The occurrence of more frequent FTCs is predicted to influence soil carbon and nitrogen cycles and increase nitrogen leaching. Biochar has the potential to increase soil organic carbon storage and decrease nitrogen leaching. This study aims to investigate the impact of freeze–thaw cycles (FTCs) on soil nitrogen leaching in temperate zones, considering the potential exacerbation of FTCs due to global climate warming and increased climate variability. This study focuses on how biochar, a carbon-rich material produced from biomass, might mitigate nitrogen leaching by influencing soil characteristics. This study explores the interactions between different laboratory-simulated FTC frequencies (ranging from 0 to 12 cycles) and various biochar addition ratios (0%, 2%, 4%, and 6% w/w) on soil nitrogen leaching based on a total of 60 soil columns. Pearson correlations between the soil quality indicators and nitrogen leaching characteristics were detected, and partial least squares path modeling (PLS-PM) was used to assess the effects of the FTCs, biochar addition ratios, and soil quality indicators on the nitrogen leaching content. The results showed that the amount of leached soil NH4+-N and NO3−-N reached 0.129–1.726 mg and 2.90–7.90 mg, respectively. NH4+-N and NO3−-N first increased and then decreased under the FTCs, with the highest values being observed after the 6th FTC. As the biochar addition ratio increased, the NH4+-N and NO3−-N contents decreased. Correlation analysis showed that the nitrogen leaching content was significantly related to the soil pH, soil organic matter (SOM), NH4+-N content, and microbial biomass carbon content (MBC) (p &lt; 0.01). The results of the conceptual path model revealed that nitrogen leaching characteristics were significantly affected by the pH, SOM, soil nitrogen content, and biochar addition ratio. Our results suggest that biochar addition can help reduce nitrogen leaching in farmland soil in areas with black soil and seasonal freeze–thaw cycles.

Effects of freeze-thaw on soil detachment capacity in the black soil region of Northeastern China

Soil detachment capacity (Dc) is an important parameter used to determine erosion intensity in physical-process-based erosion models. Freeze-thaw affects soil detachment processes by altering the mechanical properties of soil; however, due to the compound action of freeze-thaw and runoff on Dc, quantifying the impact of seasonal freeze-thaw on Dc remains challenging. A series of experiments with six freeze-thaw cycles (FTC), six initial soil moisture contents (SMC), three slope gradients, and five flow discharges were conducted to investigate the effect of freeze-thaw and hydrodynamic characteristics on Dc. The results showed that soil shear strength (τm), cohesion (Coh), and internal friction angle (φ) gradually tended to become stable with increasing FTC, indicating that repeated FTC had a cumulative impact on soil mechanical properties, and there was a critical FTC between 5 and 7. When FTC rose from 1 to 15, the reduction in τm, Coh, and φ was 0.03∼23.96%, 2.63∼75.21%, and − 5.70∼19.24%, respectively, which increased with an increasing SMC, suggesting that the deterioration effect of FTC on soil mechanical properties was promoted by increasing SMC. During alternating FTC, the relative range and variation coefficient of Dc were 2.21–2.43 and 67.87–75.72%, respectively, indicating that Dc was highly sensitive to FTC. Furthermore, Dc increased by 2.37–71.22% after 15 FTC. Alternating freeze-thaw weakened the soil resistance to detachment. Moreover, the promoting effect of FTC on Dc intensified with an increasing SMC, indicating that the variation in Dc was strongly affected by SMC during FTC. A prediction model (R2=0.955, RRMSE=14.99%) was established to quantify the influence of freeze-thaw and hydrodynamic characteristics on Dc. The explanation rate of variables in the Dc prediction equation was quantitated: the explanation rate of stream power (64.3%) was higher than that of FTC (10.02%) and SMC (3.92%), suggesting that the impact of freeze-thaw on Dc was covered by hydrodynamic characteristics. Further validation is required for the prediction equations when applied beyond the range of construction conditions.

Soil bacterial communities in alpine wetlands in arid Central Asia remain stable during the seasonal freeze–thaw period

Under the background of climate change, freeze–thaw patterns tend to be turbulent: ecosystem function processes and their mutual feedback mechanisms with microorganisms in sensitive areas around the world are currently a hot topic of research. We studied changes of soil properties in alpine wetlands located in arid areas of Central Asia during the seasonal freeze–thaw period (which included an initial freezing period, a deep freezing period, and a thawing period), and analyzed changes in soil bacterial community diversity, structure, network in different stages with the help of high-throughput sequencing technology. The results showed that the α diversity of the soil bacterial community showed a continuous decreasing trend during the seasonal freeze–thaw period. The relative abundance of dominant bacterial groups (Proteobacteria (39.04%–41.28%) and Bacteroidota (14.61%–20.12%)) did not change significantly during the freeze–thaw period. At the genus level, different genera belonging to the same phylum dominated in different stages, or there were clusters of genera belonging to different phylum. For example, g_Ellin6067, g_unclassified_f_Geobacteraceae, g_unclassified_f_Gemmatimonadaceae coexisted in the same cluster, belonging to Proteobacteria, Desulfobacterota and Gemmatimonadota respectively, and their abundance increased significantly during the freezing period. This “adaptive freeze–thaw” phylogenetic model suggests a heterogeneous stress resistance of bacteria during the freeze–thaw period. In addition, network analysis showed that, although the bacterial network was affected to some extent by environmental changes during the initial freezing period and its recovery in the thawing period lagged behind, the network complexity and stability did not change much as a whole. Our results prove that soil bacterial communities in alpine wetlands are highly resistant and adaptive to seasonal freeze–thaw conditions. As far as we know, compared with short-term freeze–thaw cycles research, this is the first study examining the influence of seasonal freeze–thaw on soil bacterial communities in alpine wetlands. Overall, our findings provide a solid base for further investigations of biogeochemical cycle processes under future climate change.

Effects of freeze-thaw cycles on the size distribution and stability of soil aggregate in the permafrost regions of the Qinghai-Tibetan Plateau

As the basic units of soil structure, soil aggregate is essential for maintaining soil stability. Intensified freeze–thaw cycles have deeply affected the size distribution and stability of aggregate under global warming. To date, it is still lacking about the effects of freeze–thaw cycles on aggregate in the permafrost regions of the Qinghai-Tibetan Plateau (QTP). Therefore, we investigated the effects of diurnal and seasonal freeze–thaw processes on soil aggregate. Our results showed that the durations of thawing and freezing periods in the 0–10 cm layer were longer than in the 10–20 cm layer, while the opposite results were observed during completely thawed and frozen periods. Freeze–thaw strength was greater in the 0–10 cm layer than that in the 10–20 cm layer. The diurnal freeze–thaw cycles have no significant effect on the size distribution and stability of aggregate. However, < 0.25 mm fraction dominated wet sieving aggregate with the highest proportion during thawing period, while the < 1 mm fraction reached the highest during completely frozen period in the 10–20 cm layer (P < 0.05). Likewise, the mean weight diameter and water-stable aggregate were decreased during thawing period compared with the other periods, which were influenced by soil microbial biomass carbon and belowground biomass. Hence, the seasonal freeze–thaw processes destroyed macro-aggregate (> 0.25 mm) and reduced aggregate stability. Our study has scientific guidance for evaluating the effects of freeze–thaw cycles on soil steucture and provides a theoretical basis for further exploration on soil and water conservation in the permafrost regions of the QTP.

Seasonal freeze–thaw processes regulate and buffer the distribution of microbial communities in soil horizons

The freeze–thaw process alters microbial communities in alpine soils. However, soil microbial responses to seasonal freeze–thaw processes have been overlooked under climate change, especially in deep soil. In this study, microbial samples were collected from three alpine meadows on the northeastern Qinghai-Tibet Plateau during four seasonal freeze–thaw periods (TP: thawed period; UFP: unstable freezing period; FP: frozen period; and UTP: unstable thawing period), and the taxa and biomass of living microbes were determined by the phospholipid fatty acid (PLFA) method. The results showed that the microbial biomass in the TP was significantly lower than that in other seasonal freeze–thaw periods. The biomass of fungi and gram-positive bacteria (GP) was high in the UFP and FP, and the biomass of bacteria and gram-negative bacteria (GN) was high in the UTP. The soil microbial biomass decreased with soil depth, and the difference in soil microbial biomass between soil horizons in the FP was smaller than those in the UTP, FP and UFP. The values of GN/GP and fungi/bacteria (F/B) decreased with soil depth during all seasonal freeze–thaw periods and were lower in the UFP and FP than in the TP and UTP. The main controlling factors of the total microbial biomass of the alpine meadow were soil temperature, soil organic carbon (SOC) and total nitrogen (TN) during the seasonal freeze–thaw periods. TN was the critical soil variable affecting the total microbial biomass in the TP. SOC was the critical soil variable affecting the total microbial biomass in the UFP and UTP. With global warming (especially in winter), soil microbial biomass can be expected to decrease in alpine regions. These results could provide a deep understanding of biodiversity and the prediction of carbon cycle in alpine regions.

Simulation and Analysis of the Dynamic Characteristics of Groundwater in Taliks in the Eruu Area, Central Yakutia

The perennially unfrozen zones (taliks) in the Eruu area of central Yakutia have a complex stratigraphic structure, and the dynamic characteristics of groundwater in this region have been insufficiently studied. This study analyzed the results of the explorations and geophysical studies conducted by the Melnikov Permafrost Institute of the Siberian Branch of the Russian Academy of Science. In addition, we simulated and analyzed the dynamic characteristics of groundwater in the area based on hydro-meteorological data, snow data, and remote sensing data. During the process, the dynamic changes in the attributes of aquifers due to the seasonal freeze–thaw processes of soils, including the active layer, were also taken into account. The results showed the following: (1) According to the analysis of the measured data on water levels in hydrogeological observation well 14E/2014, the difference between the simulated and measured values of groundwater levels in monitoring wells for over 99% of the measurements was less than 0.1 m. The average difference between the measured (excluding missing values) and simulated values of groundwater level in the monitoring wells was 0.028 m/d. (2) The annual average water level in the study area declined. The simulated value dropped at a rate of 0.10 m/a, with only a gap of 0.01 m/a with the measured value. Meanwhile, the simulated water head was greatly influenced by the terrain, especially in the central area, where the head decreased rapidly from the perimeter toward the lakes (8.9 m/km on average). (3) From 1 September 2014 to 31 August 2015, the mean value of the simulated discharge in the study area was 3888.39 L/d, which was in line with the results of previous monitoring (the average flow was 4147.20 L/d and 3715.20 L/d in 2014 and 2015, respectively). This study can provide a reference for the reasonable exploitation and utilization of groundwater under the influence of the distribution of perennially unfrozen zones, or taliks, and provides an effective three-dimensional modeling method for quantifying the analysis of groundwater dynamics in permafrost regions.

Mechanical properties and microscopic mechanism of active MgO-fly ash solidified saline soil in seasonal freezing areas

This paper presents an innovative method for improving the strength of salt-bearing clay under freeze–thaw cycles. The active magnesium oxide (MgO) and industrial waste fly ash were employed as primary solidifying materials in the seasonal freezing soil reinforcement process, and the reinforcement effect is achieved through carbonate crystals generated through carbonization tests. The solidification effect was investigated with varying ratios of solidifying materials, salt content, and freeze–thaw cycles. The experiment results revealed that the combination of active MgO and fly ash effectively enhances the strength of salt-bearing clay. Specifically, compared to unsolidified soil, the soil solidified with 8 % active MgO (8M0F) and a combination of 4 % active MgO and 4 % fly ash (4M4F) demonstrated a significant increase in ultimate strength by 14 and 12 times, respectively. Moreover, the number of freeze–thaw cycles exhibited a negative correlation with ultimate strength, whereas the salt content demonstrated a similar negative correlation. Microscopic analysis revealed that the 8M0F sample produced mainly magnesium carbonates and hydrogen and oxygen compounds, whereas the 4M4F sample generated primarily magnesium and calcium carbonates. The resulting compound crystals efficiently filled the internal pores and cemented the soil particles, leading to a substantial increase in strength. Overall, these results indicate that the active MgO combined with fly ash solidifying method can offer a viable and sustainable solution for improving the strength of salt-bearing clay in areas with seasonal freeze–thaw cycles.

CO2 and CH4 fluxes from forest soil in the northern Da Xing’anling Mountains in Northeast China during the freezing and thawing periods of near-surface soil in 2018–2019

ABSTRACT Under a warming climate, the studies on effects of seasonal freeze–thaw cycles on soil respiration are key to understanding the soil carbon cycles and ecosystem responses and resilience at mid- and high latitudes. In this study, the effluxes of soil surface CO2 and absorption rates of CH4 were measured in the Xing'an larch (Larix gmelinii) forest in the northern Da Xing’anling Mountains, Northeast China using an automatic multichannel soil greenhouse gas measurement system (dynamic gas chamber method) during the periods of October–November 2018 and April–May 2019. The results showed that during the freezing period of near-surface soil, CO2 and CH4 fluxes significantly declined, approaching zero, at the end of November. During the thawing period of near-surface soil, CO2 and CH4 fluxes from surface soil fluctuated markedly at first and then rose rapidly. The respective Q10 values of 3.86 and 4.89 during the freezing and thawing periods of near-surface soil, respectively, indicate the important role of ground freeze–thaw cycles in the modifications of CO2 and CH4 fluxes. The results of this study could help assess the stability of the soil carbon pool and carbon flux strength in taiga-forested permafrost regions in the northern Da Xing’anling Mountains, Northeast China.